Abstract 187: Down-regulation of microRNA34 induces cell proliferation and invasion of human mesothelial cells

2012 ◽  
Author(s):  
Norimitsu Tanaka ◽  
Shinichi Toyooka ◽  
junichi soh ◽  
Kazunori Tsukuda ◽  
Kazuhiko Shien ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mesothelial Cells ◽  
Down Regulation ◽  
Proliferation And Invasion

scholarly journals Down-regulation LncRNA-SNHG15 contributes to proliferation and invasion of bladder cancer cells

BMC Urology ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Aldhabi Mokhtar ◽  
Chuize Kong ◽  
Zhe Zhang ◽  
Yan Du
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Tumor Stage ◽  
Rt Pcr ◽  
Down Regulation ◽  
Bladder Cancer Cells ◽  
Cancer Tissues ◽  
Proliferation And Invasion

Abstract Objectives The aim of this study was to investigate the effect of lncRNA-SNHG15 in bladder carcinoma using cell lines experiments and the relationship between clinical characteristics and lncRNA-SNHG15 expression was analyzed. Methods Bladder cancer tissues and near-cancer tissues were collected. The real-time PCR (RT-PCR) was used to detect the expression of lncRNA-SNHG15 in tissues and cell lines. The expression of lncRNA-SNHG15 was downregulated by interference (siRNA), as detected by RT-PCR, that was used to determine the efficiency of the interference. CCK-8 and Transwell assays were used to evaluate the effect of lncRNA-SNHG15 on the proliferation and invasion capability of bladder cancer cells. The t-test was used for Statistical analyses, which were carried out using the Statistical Graph pad 8.0.1.224 software. Result The expression of lncRNA-SNHG15 was up regulated in 5637, UMUC3 and T24 cell lines compared with corresponding normal controls (P < 0.05). Up regulation was positively related to tumor stage (P = 0.015). And tumor size (P = 0.0465). The down-regulation of lncRNA-SNHG15 with siRNA significantly inhibited UMUC3 and T24 cell proliferation and invasion. Conclusion This study showed that lncRNA-SNHG15 is overexpressed in bladder cancer tissues and (5637, UMUC3 T24) cell lines. Up regulation was positively related to tumor stage (P = 0.015), and tumor size (P = 0.0465). Down-regulation of lncRNA-SNHG15 by siRNA significantly inhibited UMUC3 and T24 cell proliferation and invasion, indicating a potential molecular target for future tumor targeted therapy.

scholarly journals Hsa_circ_0026134 expression promoted TRIM25- and IGF2BP3-mediated hepatocellular carcinoma cell proliferation and invasion via sponging miR-127-5p

2020 ◽  
Vol 40 (7) ◽  
Author(s):  
Wei Zhang ◽  
Liang Zhu ◽  
Guowei Yang ◽  
Bo Zhou ◽  
Jianhua Wang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Cell Lines ◽  
Luciferase Reporter ◽  
Circular Rnas ◽  
Down Regulation ◽  
Mrna Binding Protein ◽  
New Treatment ◽  
Mrna Binding ◽  
Proliferation And Invasion

Abstract Increasing evidence shows that circular RNAs (circRNAs) play a regulatory role in cancer. In the present study, we aimed to investigate the characteristics and effects of hsa_circ_0026134 in hepatocellular carcinoma (HCC). We investigated hsa_circ_0026134 expression in 20 pairs of clinical tissues from HCC patients; expression of hsa_circ_0026134 in different cell lines; effect of hsa_circ_0026134 on proliferation and invasion of HCC cell lines; and the regulatory mechanisms and interactions among hsa_circ_0026134, miR-127-5p, tripartite motif-containing protein 25 (TRIM25) and insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3). hsa_circ_0026134 expression was increased in HCC samples and cell lines. Down-regulation of hsa_circ_0026134 attenuated HCC cell proliferation and metastatic properties. Micro (mi)RNA (miR)-127-5p was sponged by hsa_circ_0026134. Rescue experiments indicated that inhibition of miR-127-5p expression promoted cell proliferation and invasion even after hsa_circ_0026134 silencing. TRIM25 and IGF2BP3 were targets of miR-127-5p. Overexpression of TRIM25 or IGF2BP3 promoted cell proliferation and invasion in cells overexpressing miR-127-5p. Down-regulation of hsa_circ_0026134 suppressed TRIM25- and IGF2BP3-mediated HCC cell proliferation and invasion via promotion of miR-127-5p expression, which have been confirmed by luciferase reporter assay. The present study provides a new treatment target for HCC.

scholarly journals Downregulation of microRNA-34 induces cell proliferation and invasion of human mesothelial cells

2013 ◽  
Vol 29 (6) ◽  
pp. 2169-2174 ◽  
Author(s):  
NORIMITSU TANAKA ◽  
SHINICHI TOYOOKA ◽  
JUNICHI SOH ◽  
KAZUNORI TSUKUDA ◽  
KAZUHIKO SHIEN ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mesothelial Cells ◽  
Proliferation And Invasion

Down-regulation of dishevelled-2 inhibits cell proliferation and invasion in hepatoblastoma

2018 ◽  
Vol 65 (7) ◽  
pp. e27032 ◽  
Author(s):  
Siqi Huang ◽  
Jing Chen ◽  
Ruicheng Tian ◽  
Jing Wang ◽  
Chenjie Xie ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Down Regulation ◽  
Proliferation And Invasion

scholarly journals Down-regulation of USP8 Inhibits Cholangiocarcinoma Cell Proliferation and Invasion

2020 ◽  
Vol Volume 12 ◽  
pp. 2185-2194
Author(s):  
Xu Jing ◽  
Yingjie Chen ◽  
Ye Chen ◽  
Guangyan Shi ◽  
Shuanghao Lv ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Down Regulation ◽  
Cholangiocarcinoma Cell ◽  
Proliferation And Invasion

scholarly journals Amelioration of human peritoneal mesothelial cell co-culture-evoked malignant potential of ovarian cancer cells by acacetin involves LPA release-activated RAGE-PI3K/AKT signaling

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Meng Tian ◽  
Yingjie Tang ◽  
Ting Huang ◽  
Yang Liu ◽  
Yingzheng Pan
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Inflammatory Cytokine ◽  
Mesothelial Cell ◽  
Akt Signaling ◽  
Mesothelial Cells ◽  
Ovarian Cancer Cells ◽  
Peritoneal Mesothelial Cells ◽  
Proliferation And Invasion

Abstract Background Ovarian cancer is a devastating gynecological malignancy and frequently presents as an advanced carcinoma with disseminated peritoneum metastasis. Acacetin exerts anti-cancerous effects in several carcinomas. Here, we sought to investigate acacetin function in ovarian cancer malignancy triggered by peritoneal mesothelial cells. Methods Peritoneal mesothelial cells were treated with acacetin, and then the conditioned medium was collected to treat ovarian cancer cells. Then, cell proliferation was analyzed by MTT assay. Transwell analysis was conducted to evaluate cell invasion. Protein expression was determined by western blotting. ELISA and qRT-PCR were applied to analyze inflammatory cytokine levels. The underlying mechanism was also explored. Results Acacetin suppressed cell proliferation and invasion, but enhanced cell apoptosis. Furthermore, mesothelial cell-evoked malignant characteristics were inhibited when mesothelial cells were pre-treated with acacetin via restraining cell proliferation and invasion, concomitant with decreases in proliferation-related PCNA, MMP-2 and MMP-9 levels. Simultaneously, acacetin reduced mesothelial cell-induced transcripts and production of pro-inflammatory cytokine IL-6 and IL-8 in ovarian cancer cells. Mechanically, acacetin decreased lysophosphatidic acid (LPA) release from mesothelial cells, and subsequent activation of receptor for advanced glycation end-products (RAGE)-PI3K/AKT signaling in ovarian cancer cells. Notably, exogenous LPA restored the above pathway, and offset the efficacy of acacetin against mesothelial cell-evoked malignancy in ovarian cancer cells, including cell proliferation, invasion and inflammatory cytokine production. Conclusions Acacetin may not only engender direct inhibition of ovarian cancer cell malignancy, but also antagonize mesothelial cell-evoked malignancy by blocking LPA release-activated RAGE-PI3K/AKT signaling. Thus, these findings provide supporting evidence for a promising therapeutic agent against ovarian cancer. Graphical Abstract

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