Synthesis and photophysical properties of a novel green fluorescent polymer for Fe3+sensing

2008 ◽  
Vol 57 (12) ◽  
pp. 1343-1350 ◽  
Author(s):  
Yulin Hu ◽  
Baoyan Wang ◽  
Zhixing Su
Keyword(s):  
Photophysical Properties ◽  
Fluorescent Polymer ◽  
Green Fluorescent

A Unified Model for Photophysical and Electro-Optical Properties of Green Fluorescent Proteins

2019 ◽  
Author(s):  
Chi-Yun Lin ◽  
Matthew Romei ◽  
Luke Oltrogge ◽  
Irimpan Mathews ◽  
Steven Boxer
Keyword(s):  
Driving Force ◽  
Fluorescent Protein ◽  
Charge Transfer Band ◽  
Photophysical Properties ◽  
Polymethine Dyes ◽  
Emission Rates ◽  
Unified Framework ◽  
Underlying Factor ◽  
Green Fluorescent ◽  
Protein Environment

Green fluorescent protein (GFPs) have become indispensable imaging and optogenetic tools. Their absorption and emission properties can be optimized for specific applications. Currently, no unified framework exists to comprehensively describe these photophysical properties, namely the absorption maxima, emission maxima, Stokes shifts, vibronic progressions, extinction coefficients, Stark tuning rates, and spontaneous emission rates, especially one that includes the effects of the protein environment. In this work, we study the correlations among these properties from systematically tuned GFP environmental mutants and chromophore variants. Correlation plots reveal monotonic trends, suggesting all these properties are governed by one underlying factor dependent on the chromophore's environment. By treating the anionic GFP chromophore as a mixed-valence compound existing as a superposition of two resonance forms, we argue that this underlying factor is defined as the difference in energy between the two forms, or the driving force, which is tuned by the environment. We then introduce a Marcus-Hush model with the bond length alternation vibrational mode, treating the GFP absorption band as an intervalence charge transfer band. This model explains all the observed strong correlations among photophysical properties; related subtopics are extensively discussed in Supporting Information. Finally, we demonstrate the model's predictive power by utilizing the additivity of the driving force. The model described here elucidates the role of the protein environment in modulating photophysical properties of the chromophore, providing insights and limitations for designing new GFPs with desired phenotypes. We argue this model should also be generally applicable to both biological and non-biological polymethine dyes.<br>

scholarly journals A Sustainable Biomineralization Approach for the Synthesis of Highly Fluorescent Ultra-Small Pt Nanoclusters

Biosensors ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 128 ◽  
Author(s):  
Rajkamal Balu ◽  
Robert Knott ◽  
Christopher M. Elvin ◽  
Anita J. Hill ◽  
Namita R. Choudhury ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Photoelectron Spectroscopy ◽  
Photophysical Properties ◽  
Conformational Dynamics ◽  
Aqueous Media ◽  
Intrinsically Disordered Protein ◽  
X Ray ◽  
Pt Nanoclusters ◽  
Intrinsically Disordered ◽  
Green Fluorescent

Herein we report the first example of a facile biomineralization process to produce ultra-small-sized highly fluorescent aqueous dispersions of platinum noble metal quantum clusters (Pt-NMQCs) using a multi-stimulus responsive, biomimetic intrinsically disordered protein (IDP), Rec1-resilin. We demonstrate that Rec1-resilin acts concurrently as the host, reducing agent, and stabilizer of the blue-green fluorescent Pt-NMQCs once they are being formed. The photophysical properties, quantum yield, and fluorescence lifetime measurements of the synthesized Pt-NMQCs were examined using UV-Vis and fluorescence spectroscopy. The oxidation state of the Pt-NMQCs was quantitatively analyzed using X-ray photoelectron spectroscopy. Both a small angle X-ray scattering technique and a modeling approach have been attempted to present a detailed understanding of the structure and conformational dynamics of Rec1-resilin as an IDP during the formation of the Pt-NMQCs. It has been demonstrated that the green fluorescent Pt-NMQCs exhibit a high quantum yield of ~7.0% and a lifetime of ~9.5 ns in aqueous media. The change in photoluminescence properties due to the inter-dot interactions between proximal dots and aggregation of the Pt-NMQCs by evaporation was also measured spectroscopically and discussed.

Design of Luminescent Polymers for Leds

2001 ◽  
Vol 708 ◽  
Author(s):  
A. B. Holmes ◽  
A. D. Bond ◽  
J. E. Davies ◽  
C. Fischmeister ◽  
J. Frey ◽  
...  
Keyword(s):  
Luminescent Polymers ◽  
Fluorescent Polymers ◽  
Fluorescent Polymer ◽  
Green Fluorescent

ABSTRACTA 2,3-dibutoxy-1,4-phenylenevinylene comonomer was incorporated into a distyrylbenzene derivative 11. Novel 1,2-disubstituted-3,6-dibromobenzene comonomers 15 and 18 were prepared by directed metallation. Copolymerization of 11with a 9,9-dioctylfluorene-2,7-diboronate ester 1 yielded a green fluorescent polymer while copolymerization of 15 and 18 with the 9,9-dihexylfluorene-2,7-diboronate 22 afforded promising blue fluorescent polymers 23 and 24 respectively.

Synthesis and photophysical properties of pyrene-based green fluorescent dyes: butterfly-shaped architectures

2014 ◽  
Vol 12 (40) ◽  
pp. 7914-7918 ◽  
Author(s):  
Devendar Goud Vanga ◽  
Mithun Santra ◽  
Ashok Keerthi ◽  
Suresh Valiyaveettil
Keyword(s):  
Fluorescent Dyes ◽  
Photophysical Properties ◽  
Coupling Reaction ◽  
Cross Coupling ◽  
Cross Coupling Reaction ◽  
Green Fluorescent ◽  
Fluorescent Compounds

A few pyrene-based fluorescent compounds were synthesized using Pd/Cu-catalyzed cross-coupling reaction.

scholarly journals Genetically Encoded Photosensitizers as Light-Triggered Antimicrobial Agents

2019 ◽  
Vol 20 (18) ◽  
pp. 4608 ◽  
Author(s):  
Fabienne Hilgers ◽  
Nora Lisa Bitzenhofer ◽  
Yannic Ackermann ◽  
Alina Burmeister ◽  
Alexander Grünberger ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Fluorescent Protein ◽  
Photophysical Properties ◽  
Type I ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Intracellular Expression ◽  
Global Concern ◽  
Green Fluorescent

Diseases caused by multi-drug resistant pathogens have become a global concern. Therefore, new approaches suitable for treating these bacteria are urgently needed. In this study, we analyzed genetically encoded photosensitizers (PS) related to the green fluorescent protein (GFP) or light-oxygen-voltage (LOV) photoreceptors for their exogenous applicability as light-triggered antimicrobial agents. Depending on their specific photophysical properties and photochemistry, these PSs can produce different toxic ROS (reactive oxygen species) such as O2•− and H2O2 via type-I, as well as 1O2 via type-II reaction in response to light. By using cell viability assays and microfluidics, we could demonstrate differences in the intracellular and extracellular phototoxicity of the applied PS. While intracellular expression and exogenous supply of GFP-related PSs resulted in a slow inactivation of E. coli and pathogenic Gram-negative and Gram-positive bacteria, illumination of LOV-based PSs such as the singlet oxygen photosensitizing protein SOPP3 resulted in a fast and homogeneous killing of these microbes. Furthermore, our data indicate that the ROS type and yield as well as the localization of the applied PS protein can strongly influence the antibacterial spectrum and efficacy. These findings open up new opportunities for photodynamic inactivation of pathogenic bacteria.

scholarly journals Photophysical and Photochemical Properties of Green Fluorescent Liquid Crystalline Systems

2003 ◽  
Vol 58 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Ivo Grabchev ◽  
Jean-Marc Chovelon
Keyword(s):  
Liquid Crystal ◽  
Nematic Liquid Crystal ◽  
Liquid Crystalline ◽  
Photophysical Properties ◽  
Orientational Order ◽  
Polymer Dispersed Liquid Crystal ◽  
Polymer Dispersed ◽  
Photochemical Properties ◽  
Green Fluorescent ◽  
Naphthalimide Dye

The photophysical properties of polymer-dispersed liquid crystal systems, containing naphthalimide dye as fluorescent units are reported. Investigations have been carried out on some 1,8- naphtalimide derivatives both in isotropic and anisotropic media, and their photophysal properties have been described. The orientational order parameters of the dyes in nematic liquid crystal ZLI 1840 have been determined. The photostability of the dye/liquid crystal systems has been investigated and the effect that the substituents in the naphthalimide structure have upon the orientation of the dye was discussed.

scholarly journals Utilizing Tyrosine Analogs to Alter Photophysical Properties of Green Fluorescent Protein

2020 ◽  
Vol 118 (3) ◽  
pp. 471a
Author(s):  
Darcy R. Harris ◽  
Scott H. Brewer ◽  
Christine M. Phillips-Piro
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Photophysical Properties ◽  
Green Fluorescent

Shaping the Light: The Key Factors Affecting the Photophysical Properties of Fluorescent Polymer Nanostructures

2016 ◽  
Vol 37 (24) ◽  
pp. 2037-2044 ◽  
Author(s):  
Mu-Huan Chi ◽  
Chun-Hsien Su ◽  
Ming-Hsiang Cheng ◽  
Pei-Yun Chung ◽  
Chi-How Peng ◽  
...  
Keyword(s):  
Photophysical Properties ◽  
Key Factors ◽  
Factors Affecting ◽  
Polymer Nanostructures ◽  
Fluorescent Polymer

scholarly journals A photostable monomeric superfolder GFP

2019 ◽  
Author(s):  
Fernando Valbuena ◽  
Ivy Fizgerald ◽  
Rita L. Strack ◽  
Neal Andruska ◽  
Luke Smith ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Photophysical Properties ◽  
General Purpose ◽  
Adverse Conditions ◽  
Aequorea Victoria ◽  
The Past ◽  
Nonspecific Interactions ◽  
Green Fluorescent ◽  
Partner Proteins ◽  
Protein Tagging

ABSTRACTThe green fluorescent protein GFP from Aequorea victoria has been engineered extensively in the past to generate variants suitable for protein tagging. Early efforts produced the enhanced variant EGFP and its monomeric derivative mEGFP, which have useful photophysical properties, as well as superfolder GFP, which folds efficiently under adverse conditions. We previously generated msGFP, a monomeric superfolder derivative of EGFP. Unfortunately, compared to EGFP, msGFP and other superfolder GFP variants show faster photobleaching. We now describe msGFP2, which retains monomeric superfolder properties while being as photostable as EGFP. msGFP2 contains modified N- and C-terminal peptides that are expected to reduce nonspecific interactions. Compared to EGFP and mEGFP, msGFP2 is less prone to disturbing the functions of certain partner proteins. For general-purpose protein tagging, msGFP2 may be the best available derivative of A. victoria GFP.

Structural and spectrophotometric investigation of two unnatural amino-acid altered chromophores in the superfolder green fluorescent protein

2021 ◽  
Vol 77 (8) ◽  
Author(s):  
Gregory M. Olenginski ◽  
Juliana Piacentini ◽  
Darcy R. Harris ◽  
Nicolette A. Runko ◽  
Brianna M. Papoutsis ◽  
...  
Keyword(s):  
Amino Acids ◽  
Green Fluorescent Protein ◽  
Amino Acid ◽  
Unnatural Amino Acids ◽  
Fluorescent Protein ◽  
Photophysical Properties ◽  
Unnatural Amino Acid ◽  
Green Fluorescent ◽  
Absorbance Band ◽  
Protein Constructs

The spectrophotometric properties of the green fluorescent protein (GFP) result from the post-translationally cyclized chromophore composed of three amino acids including a tyrosine at the center of the β-barrel protein. Altering the amino acids in the chromophore or the nearby region has resulted in numerous GFP variants with differing photophysical properties. To further examine the effect of small atomic changes in the chromophore on the structure and photophysical properties of GFP, the hydroxyl group of the chromophore tyrosine was replaced with a nitro or a cyano group. The structures and spectrophotometric properties of these superfolder GFP (sfGFP) variants with the unnatural amino acids (UAAs) 4-nitro-L-phenylalanine or 4-cyano-L-phenylalanine were explored. Notably, the characteristic 487 nm absorbance band of wild-type (wt) sfGFP is absent in both unnatural amino-acid-containing protein constructs (Tyr66pNO2Phe-sfGFP and Tyr66pCNPhe-sfGFP). Consequently, neither Tyr66pNO2Phe-sfGFP nor Tyr66pCNPhe-sfGFP exhibited the characteristic emission of wt sfGFP centered at 511 nm when excited at 487 nm. Tyr66pNO2Phe-sfGFP appeared orange due to an absorbance band centered at 406 nm that was not present in wt sfGFP, while Tyr66pCNPhe-sfGFP appeared colorless with an absorbance band centered at 365 nm. Mass spectrometry and X-ray crystallography confirmed the presence of a fully formed chromophore and no significant structural changes in either of these UAA-containing protein constructs, signaling that the change in the observed photophysical properties of the proteins is the result of the presence of the UAA in the chromophore.

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