Chronic toxicity of clothianidin, imidacloprid, chlorpyrifos, and dimethoate to Apis mellifera L. larvae reared in vitro

2018 ◽  
Vol 75 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Pingli Dai ◽  
Cameron J Jack ◽  
Ashley N Mortensen ◽  
Tomas A Bustamante ◽  
Jeffrey R Bloomquist ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Chronic Toxicity

scholarly journals Chronic toxicity of amitraz, coumaphos and fluvalinate to Apis mellifera L. larvae reared in vitro

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Pingli Dai ◽  
Cameron J. Jack ◽  
Ashley N. Mortensen ◽  
Tomas A. Bustamante ◽  
James D. Ellis
Keyword(s):  
Apis Mellifera ◽  
Chronic Toxicity

Acute and chronic toxicity of acetamiprid, carbaryl, cypermethrin and deltamethrin to Apis mellifera larvae reared in vitro

2019 ◽  
Vol 76 (3) ◽  
pp. 978-985 ◽  
Author(s):  
Yang Yang ◽  
Shilong Ma ◽  
Feng Liu ◽  
Qiang Wang ◽  
Xing Wang ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Chronic Toxicity ◽  
Acute And Chronic Toxicity

scholarly journals Synthesis, Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2543
Author(s):  
Ruidong Ni ◽  
Suzeeta Bhandari ◽  
Perry R. Mitchell ◽  
Gabriela Suarez ◽  
Neel B. Patel ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Fatty Acid ◽  
Apis Mellifera ◽  
Chemical Synthesis ◽  
Acid Amide ◽  
Fatty Acid Amides ◽  
Fatty Acid Amide

Fatty acid amides are a diverse family of underappreciated, biologically occurring lipids. Herein, the methods for the chemical synthesis and subsequent characterization of specific members of the fatty acid amide family are described. The synthetically prepared fatty acid amides and those obtained commercially are used as standards for the characterization and quantification of the fatty acid amides produced by biological systems, a fatty acid amidome. The fatty acid amidomes from mouse N18TG2 cells, sheep choroid plexus cells, Drosophila melanogaster, Bombyx mori, Apis mellifera, and Tribolium castaneum are presented.

scholarly journals Effects of a commercially formulated glyphosate solutions at recommended concentrations on honeybee (Apis mellifera L.) behaviours

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Qi-Hua Luo ◽  
Jing Gao ◽  
Yi Guo ◽  
Chang Liu ◽  
Yu-Zhen Ma ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Theoretical Basis ◽  
Chronic Toxicity ◽  
Sublethal Effects ◽  
Significant Decline ◽  
Learning Ability ◽  
Notification System ◽  
Climbing Ability ◽  
Behavioural Experiments ◽  
Sucrose Responsiveness

AbstractGlyphosate, the active ingredient of the most widely used commercial herbicide formulation, is extensively used and produced in China. Previous studies have reported sublethal effects of glyphosate on honeybees. However, the effects of commercially formulated glyphosate (CFG) at the recommended concentration (RC) on the chronic toxicity of honeybees, especially on their behaviours, remain unknown. In this study, a series of behavioural experiments were conducted to investigate the effects of CFG on honeybees. The results showed that there was a significant decline in water responsiveness at 1/2 × , 1 × and 2 × the RC after 3 h of exposure to CFG for 11 days. The CFG significantly reduced sucrose responsiveness at 1/2 × and 1 × the RC. In addition, CFG significantly affected olfactory learning ability at 1/2 × , 1 × , and 2 × the RC and negatively affected memory ability at 1/2 × and 1 × the RC. The climbing ability of honeybees also significantly decreased at 1/2 × , 1 × and 2 × the RC. Our findings indicated that, after they were chronically exposed to CFG at the RC, honeybees exhibited behavioural changes. These results provide a theoretical basis for regulating field applications of CFG, which is necessary for establishing an early warning and notification system and for protecting honeybees.

Optimisation of an in vitro larvae test to test the effects of plant protection products on honeybee brood (Apis mellifera L.)

2014 ◽  
Vol 9 (2) ◽  
pp. 157-166 ◽  
Author(s):  
Dorothee J. Lüken ◽  
Martina Janke ◽  
Friedrich-Wilhelm Lienau ◽  
Katharina Gerdel ◽  
Werner von der Ohe ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Plant Protection ◽  
Plant Protection Products

Acute toxicity of five pesticides to Apis mellifera larvae reared in vitro

2017 ◽  
Vol 73 (11) ◽  
pp. 2282-2286 ◽  
Author(s):  
Pingli Dai ◽  
Cameron J Jack ◽  
Ashley N Mortensen ◽  
James D Ellis
Keyword(s):  
Apis Mellifera ◽  
Acute Toxicity

scholarly journals Evaluation of mutagenicity, genotoxicity and chronic toxicity of antiviral drug imidazolyl ethanamide pentandioic acid in in vitro and in vivo test systems

2021 ◽  
Vol 98 (5) ◽  
pp. 548-557
Author(s):  
E. A. Jain ◽  
D. Pleimes ◽  
A. A. Globenko
Keyword(s):  
Oral Administration ◽  
Chromosomal Aberrations ◽  
Ames Test ◽  
Chronic Toxicity ◽  
Micronucleus Test ◽  
Human Lymphocytes ◽  
Antiviral Drug ◽  
Systemic Exposure

Introduction. The antiviral properties of imidazolyl ethanamide pentandioic acid (IPA), the active compound of the drug product, has been proven in various experimental models. However, the literature data on the toxicological properties of IPA are limited.Purpose. To evaluate mutagenic and genotoxic properties in in vitro and in vivo models, as well as to study the toxicity of IPA following chronic oral administration to rats and dogs.Materials and methods. Mutagenic and genotoxic properties of IPA were assessed using the Ames test, the test of chromosomal aberrations in human lymphocytes, and the micronucleus test in rats. The chronic toxicity of IPA was studied in Sprague Dawley rats and beagle dogs of both sexes, to which IPA was administered orally at doses of 30-300 mg/kg/day for 26 and 39 weeks, respectively.Results and discussion. In the Ames test, the addition of IPA up to the maximum dose (5000 mcg/plate) did not result in the increase in the number of revertant colonies. At a concentration of up to 5000 mcg/ml, IPA did not cause chromosomal aberrations in human leukocytes. At doses doses ≤ 2000 mg/kg, IPA did not increase the amount of micronuclei in the bone marrow of rats. In chronic experiments, animals tolerated the administration of IPA well: the dose without an observed effect (NOEL) for rats and dogs was 300 mg/kg/day.Conclusion. IPA did not show mutagenic and genotoxic properties in standard in vitro and in vivo tests. With chronic oral administration to rats and dogs, NOEL IPA equal to 300 mg/kg/day provided a systemic exposure that was 8-10 and 41-65 times higher than that in humans, respectively. The results obtained allow us to consider the safety profile of the prolonged use in humans as favorable.

scholarly journals Toxicity Evaluation of Selected Plant Water Extracts on a Honey Bee (Apis mellifera L.) Larvae Model

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 178
Author(s):  
Roksana Kruszakin ◽  
Paweł Migdal
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Larval Rearing ◽  
Distilled Water ◽  
Chelidonium Majus ◽  
Freeze Dried ◽  
Study Laboratory ◽  
The Impact

So far, larval rearing in vitro has been an important method in the assessment of bee toxicology, particularly in pesticide risk assessment. However, natural products are increasingly used to control honey bee pathogens or to enhance bee immunity, but their effects on honey bee larvae are mostly unknown. In this study, laboratory studies were conducted to determine the effects of including selected aqueous plant infusions in the diet of honey bee (Apis mellifera L.) larvae in vitro. The toxicity of infusions from three different plant species considered to be medicinal plants was evaluated: tansy (Tanacetum vulgare L.), greater celandine (Chelidonium majus L.), and coriander (Coriandrum sativum L.). The impact of each on the survival of the larvae of honey bees was also evaluated. One-day-old larvae were fed a basal diet consisting of distilled water, sugars (glucose and fructose), yeast extract, and freeze-dried royal jelly or test diets in which distilled water was replaced by plant infusions. The proportion of the diet components was adjusted to the age of the larvae. The larvae were fed twice a day. The experiment lasted seven days. Significant statistical differences in survival rates were found between groups of larvae (exposed or not to the infusions of tansy, greater celandine, and coriander). A significant decrease (p < 0.05) in the survival rate was observed in the group with the addition of a coriander herb infusion compared to the control. These results indicate that plant extracts intended to be used in beekeeping should be tested on all development stages of honey bees.

scholarly journals Actividad antifúngica de propóleos obtenidos en tres provincias de Cuba sobre hongos contaminantes en cultivo de tejidos vegetales

2014 ◽  
Vol 23 (6) ◽  
pp. 3-9
Author(s):  
René Dionisio Cupull-Santana ◽  
Remigio Cortés-Rodríguez ◽  
Ervelio Eliseo Olazábal-Manso ◽  
Carlos Alberto Hernández Medina
Keyword(s):  
Apis Mellifera ◽  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp

La investigación se realizó con el objetivo de evaluar las características fisicoquímicas y la actividad antifúngica de propóleos de Apis mellifera, provenientes de las provincias de Villa Clara, Cienfuegos y Sancti Spíritus, Cuba. Se utilizó el método de recolección por raspado y se estableció el contenido de cera, ceniza, material insoluble y resina de los propóleos crudos. Además, se evaluó la actividad antimicrobiana in vitro frente a los hongos Aspergillus sp., Penicillium sp., Fusarium sp., Trichoderma sp., Colletotrichum sp. y Monilia sp.; los extractos presentaron alta actividad antifúngica en un amplio rango de concentraciones; además, los parámetros fisicoquímicos presentaron valores semejantes y dentro de los establecidos por regulaciones internacionales.

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