Cytotoxic activity of carotenoid rich fractions from Haematococcus pluvialis and Dunaliella salina microalgae and the identification of the phytoconstituents using LC-DAD/ESI-MS

2017 ◽  
Vol 32 (2) ◽  
pp. 298-304 ◽  
Author(s):  
Farouk K. El-Baz ◽  
Rehab A. Hussein ◽  
Khaled Mahmoud ◽  
Sayeda M. Abdo
Keyword(s):  
Cytotoxic Activity ◽  
Dunaliella Salina ◽  
Haematococcus Pluvialis ◽  
Esi Ms

scholarly journals Variability in Macro- and Micronutrients of 15 Commercially Available Microalgae Powders

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 310
Author(s):  
Fabian Sandgruber ◽  
Annekathrin Gielsdorf ◽  
Anja C. Baur ◽  
Benjamin Schenz ◽  
Sandra Marie Müller ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dunaliella Salina ◽  
Haematococcus Pluvialis ◽  
Saturated Fatty Acids ◽  
Dry Weight ◽  
Chlorella Pyrenoidosa ◽  
Essential Amino Acids ◽  
Arthrospira Platensis ◽  
Omega 3 ◽  
Tetraselmis Chuii

The nutrient composition of 15 commercially available microalgae powders of Arthrospira platensis, Chlorella pyrenoidosa and vulgaris, Dunaliella salina, Haematococcus pluvialis, Tetraselmis chuii, and Aphanizomenon flos-aquae was analyzed. The Dunaliella salina powders were characterized by a high content of carbohydrates, saturated fatty acids (SFAs), omega-6-polyunsaturated fatty acids (n6-PUFAs), heavy metals, and α-tocopherol, whereas the protein amounts, essential amino acids (EAAs), omega-3-PUFAs (n3-PUFAs), vitamins, and minerals were low. In the powder of Haematococcus pluvialis, ten times higher amounts of carotenoids compared to all other analyzed powders were determined, yet it was low in vitamins D and E, protein, and EAAs, and the n6/n3-PUFAs ratio was comparably high. Vitamin B12, quantified as cobalamin, was below 0.02 mg/100 g dry weight (d.w.) in all studied powders. Based on our analysis, microalgae such as Aphanizomenon and Chlorella may contribute to an adequate intake of critical nutrients such as protein with a high content of EAAs, dietary fibers, n3-PUFAs, Ca, Fe, Mg, and Zn, as well as vitamin D and E. Yet, the nutritional value of Aphanizomenon flos-aquae was slightly decreased by high contents of SFAs. The present data show that microalgae are rich in valuable nutrients, but the macro- and micronutrient profiles differ strongly between and within species.

scholarly journals Phenolic profiling of Rorippa palustris (L.) Besser (Brassicaceae) by LC-ESI-MS: Chemosystematic significance and cytotoxic activity

2016 ◽  
Vol 6 (8) ◽  
pp. 633-637 ◽  
Author(s):  
Mona Mohamed Marzouk ◽  
Sameh Reda Hussein ◽  
Ahmed Elkhateeb ◽  
Mai Mohamed Farid ◽  
Lamyaa Fawzy Ibrahim ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Esi Ms

scholarly journals Chemical Constituents from an Endophytic Fungus Chaetomium Globosum Z1

2013 ◽  
Vol 8 (9) ◽  
pp. 1934578X1300800
Author(s):  
Chun-Yan Zhang ◽  
Xiao Ji ◽  
Xuan Gui ◽  
Bao-Kang Huang
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Endophytic Fungus ◽  
Chemical Constituents ◽  
2D Nmr ◽  
Chaetomium Globosum ◽  
Spectroscopic Methods ◽  
Esi Ms

A new ergosterol, 15β-hydroxyl-(22 E,24 R)-ergosta-3,5,8,22-tetraen-one (1), along with three known ergosterols, two known cytochalasins, and two known azapholines were isolated from Chaetomium globosum Z1. The structures of these compounds were elucidated on the basis of spectroscopic methods (HR-ESI-MS, 1D NMR, and 2D NMR). Compound 6 showed significant cytotoxic activity against A-549 and MG-63 cell lines with IC50 values of 6.96 and 1.73 μg/mL, respectively.

scholarly journals UPLC-ESI-MS/MS Profile and Antioxidant, Cytotoxic, Antidiabetic, and Antiobesity Activities of the Aqueous Extracts of Three Different Hibiscus Species

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Hanan M. Al-Yousef ◽  
Wafaa H. B. Hassan ◽  
Sahar Abdelaziz ◽  
Musarat Amina ◽  
Rasha Adel ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Aqueous Extract ◽  
Biological Activities ◽  
Biological Effects ◽  
Aqueous Extracts ◽  
Potent Effect ◽  
Esi Ms ◽  
Biological Studies ◽  
Hct 116

The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50 = 50 ± 5.1 μg/mL, and Hibiscus micranthus showed a close effect with IC50 = 60.4 ± 1.7 μg/mL. Hibiscus micranthus showed the most potent effect on HCT-116 (human colon carcinoma) with IC50 = 56 ± 1.9 μg/mL compared with cisplatin (IC50 = 7.53 ± 3.8 μg/mL). HcA and HdA extracts showed weak cytotoxic activity against A-549 and HCT-116 cell lines compared to the other extracts. Eventually, Hibiscus deflersii showed astonishing antidiabetic (IC50 = 56 ± 1.9 μg/mL) and antiobesity (IC50 = 95.45 ± 1.9 μg/mL) activities using in vitro α-amylase inhibitory assay (compared with acarbose (IC50 = 34.71 ± 0.7 μg/mL)) and pancreatic lipase inhibitory assay (compared with orlistat (IC50 = 23.8 ± 0.7 μg/mL)), respectively. In conclusion, these findings are regarded as the first vision of the phytochemical constituents and biological activities of different Hibiscus aqueous extracts. Hibiscus deflersii aqueous extract might be a hopeful origin of functional constituents with anticancer (on A-549 cell line), antidiabetic, and antiobesity activities. It might be a natural alternative remedy and nutritional policy for diabetes and obesity treatment without negative side effects. Isolation of the bioactive phytochemicals from the aqueous extracts of aerial parts of Hibiscus calyphyllus, Hibiscus micranthus, and Hibiscus deflersii and estimation of their biological effects are recommended in further studies.

scholarly journals Microalgae-Derived Pigments: A 10-Year Bibliometric Review and Industry and Market Trend Analysis

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3406 ◽  
Author(s):  
Samara C. Silva ◽  
Isabel C. F. R. Ferreira ◽  
Madalena M. Dias ◽  
M. Filomena Barreiro
Keyword(s):  
Spirulina Platensis ◽  
Dunaliella Salina ◽  
Haematococcus Pluvialis ◽  
Market Analysis ◽  
Microalgae Cultivation ◽  
Natural Pigments ◽  
Pigment Extraction ◽  
Bibliometric Review ◽  
Synthetic Pigments ◽  
Β Carotene

Microalgae productive chains are gaining importance as sustainable alternatives to obtain natural pigments. This work presents a review on the most promising pigments and microalgal sources by gathering trends from a 10-year bibliometric survey, a patents search, and an industrial and market analysis built from available market reports, projects and companies’ webpages. The performed analysis pointed out chlorophylls, phycocyanin, astaxanthin, and β-carotene as the most relevant pigments, and Chlorella vulgaris, Spirulina platensis, Haematococcus pluvialis, and Dunaliella salina, respectively, as the most studied sources. Haematococcus is referred in the highest number of patents, corroborating a high technological interest in this microalga. The biorefinery concept, investment in projects and companies related to microalgae cultivation and/or pigment extraction is increasingly growing, particularly, for phycocyanin from Spirulina platensis. These pieces of evidence are a step forward to consolidate the microalgal pigments market, which is expected to grow in the coming years, increasing the prospects of replacing synthetic pigments by natural counterparts.

scholarly journals Cytotoxic activity of Moroccan Melissa officinalis leaf extracts and HPLC-ESI-MS analysis of its phytoconstituents

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Farid Khallouki ◽  
Andrea Breuer ◽  
Mourad Akdad ◽  
Fatima Ezzahra Laassri ◽  
Mohammed Attaleb ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Melissa Officinalis ◽  
Leaf Extracts ◽  
Esi Ms ◽  
Ms Analysis

scholarly journals Analgesic, Anti-Inflammatory, Cytotoxic Activity Screening and UPLC-PDA-ESI-MS Metabolites Determination of Bioactive Fractions of Kleinia pendula

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 418 ◽  
Author(s):  
Mohammad Alfaifi ◽  
Abdulrhman Alsayari ◽  
Narasimman Gurusamy ◽  
Justin Louis ◽  
Serag Eldin Elbehairi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Plant Metabolites ◽  
Esi Ms ◽  
Anti Inflammatory

Kleinia pendula (Forssk.) DC. is a prostrate or pendent dark green succulent herb found in the southwestern mountain regions of Saudi Arabia. The literature survey of the plant reveals a lack of phytochemical and pharmacological studies, although traditional uses have been noted. The objective of the present work was to assess the in vivo analgesic and anti-inflammatory activities, as well as, the in vitro cytotoxic potential of the fractions of Kleinia pendula, and correlate these activities to the plant metabolites. The methanolic extract of Kleinia pendula was subjected to fractionation with n-hexane, ethyl acetate, chloroform, n-butanol, and water. The fractions were screened for their analgesic and anti-inflammatory activities, as well as cytotoxic activity against breast, liver, and colon cancer cell lines. The n-hexane and chloroform fractions of Kleinia pendula showed significant cytotoxic activity against all three cancer cell lines tested. The ethyl acetate and chloroform fractions showed significant analgesic and anti-inflammatory activities. The metabolites in these three active fractions were determined using UPLC-PDA-ESI-MS. Thus, the analgesic and anti-inflammatory activities of the plant were attributed to its phenolic acids (caffeoylquinic acid derivatives, protocatechuic, and chlorogenic acids). While fatty acids and triterpenoids such as (tormentic acid) in the hexane fraction are responsible for the cytotoxic activity; thus, these fractions of Kleinia pendula may be a novel source for the development of new plant-based analgesic, anti-inflammatory, and anticancer drugs.

Identifying novel salt-tolerant genes from Dunaliella salina using a Haematococcus pluvialis expression system

2014 ◽  
Vol 117 (1) ◽  
pp. 113-124 ◽  
Author(s):  
Wen-fang Gong ◽  
Li-na Zhao ◽  
Bin Hu ◽  
Xi-wen Chen ◽  
Feng Zhang ◽  
...  
Keyword(s):  
Dunaliella Salina ◽  
Haematococcus Pluvialis ◽  
Expression System ◽  
Salt Tolerant
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