scholarly journals Enzyme Immobilization: Enhanced Enzyme Activity through Scaffolding on Customizable Self‐Assembling Protein Filaments (Small 20/2019)

Small ◽  
2019 ◽  
Vol 15 (20) ◽  
pp. 1970104
Author(s):  
Samuel Lim ◽  
Gi Ahn Jung ◽  
Dominic J. Glover ◽  
Douglas S. Clark
Keyword(s):  
Enzyme Activity ◽  
Enzyme Immobilization ◽  
Self Assembling

Enzyme Immobilization on Nanomaterials for Biosensor and Biocatalyst in Food and Biomedical Industry

2019 ◽  
Vol 25 (24) ◽  
pp. 2661-2676 ◽  
Author(s):  
Sundaresan Bhavaniramya ◽  
Ramar Vanajothi ◽  
Selvaraju Vishnupriya ◽  
Kumpati Premkumar ◽  
Mohammad S. Al-Aboody ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Enzyme Immobilization ◽  
Food Industry ◽  
Environmental Changes ◽  
Immobilized Enzymes ◽  
Food Industries ◽  
Physiological Processes ◽  
Different Types ◽  
Simple Processing ◽  
Biomedical Industry

Enzymes exhibit a great catalytic activity for several physiological processes. Utilization of immobilized enzymes has a great potential in several food industries due to their excellent functional properties, simple processing and cost effectiveness during the past decades. Though they have several applications, they still exhibit some challenges. To overcome the challenges, nanoparticles with their unique physicochemical properties act as very attractive carriers for enzyme immobilization. The enzyme immobilization method is not only widely used in the food industry but is also a component methodology in the pharmaceutical industry. Compared to the free enzymes, immobilized forms are more robust and resistant to environmental changes. In this method, the mobility of enzymes is artificially restricted to changing their structure and properties. Due to their sensitive nature, the classical immobilization methods are still limited as a result of the reduction of enzyme activity. In order to improve the enzyme activity and their properties, nanomaterials are used as a carrier for enzyme immobilization. Recently, much attention has been directed towards the research on the potentiality of the immobilized enzymes in the food industry. Hence, the present review emphasizes the different types of immobilization methods that is presently used in the food industry and other applications. Various types of nanomaterials such as nanofibers, nanoflowers and magnetic nanoparticles are significantly used as a support material in the immobilization methods. However, several numbers of immobilized enzymes are used in the food industries to improve the processing methods which not only reduce the production cost but also the effluents from the industry.

Moving into Nanotechnology Roles to Mimic and Boost Enzyme Activity

2018 ◽  
pp. 421-440
Author(s):  
Maria Laura Soriano
Keyword(s):  
Enzyme Activity ◽  
Catalytic Activity ◽  
Enzyme Immobilization ◽  
Mesoporous Materials ◽  
Specific Activity ◽  
Artificial Enzymes ◽  
Catalytic Nanoparticles ◽  
Solid Liquid ◽  
By Products ◽  
Insight Into

A new tendency toward the design of artificial enzymes based on nanostructures (nanodots, nanofibers, mesoporous materials) has emerged. On one hand, nanotechnology bestows self-catalytic nanoparticles with a specific activity to achieve efficient reactions with low number of by-products. On other hand, the nanoparticles may behave as nanometric scaffolds for hosting enzymes, promoting their catalytic activity and stability. In this case, enzyme immobilization requires the preservation of the catalytic activity by preventing enzyme unfolding and avoiding its aggregation. These approaches render many other advantages like hosting/storing enzymes in nanotechnological solid, liquid, and gel-like media. This chapter focuses on the most up-to-date approaches to manipulate or mimic enzyme activity based on nanotechnology, and offers examples of their applications in the most promising fields. It also gives new insight into the creation of reusable nanotechnological tools for enzyme storage.

Self-assembled nano-aggregates of fluorinases demonstrate enhanced enzymatic activity, thermostability and reusability

2020 ◽  
Vol 8 (2) ◽  
pp. 648-656
Author(s):  
Chunhao Tu ◽  
Jin Zhou ◽  
Lei Peng ◽  
Shuli Man ◽  
Long Ma
Keyword(s):  
Aqueous Solution ◽  
Enzyme Activity ◽  
Enzymatic Activity ◽  
Self Assembling ◽  
Self Assembled

Three SAP (self-assembling peptide)-tagged fluorinases (FLAs) are successfully prepared. All three SAP-tagged FLAs bear enzymatic activity and they form nano-sized particles in aqueous solution. One of them displays improved enzyme activity, thermostability and reusability.

scholarly journals Enhanced Enzyme Activity through Scaffolding on Customizable Self‐Assembling Protein Filaments

Small ◽  
2019 ◽  
Vol 15 (20) ◽  
pp. 1805558 ◽  
Author(s):  
Samuel Lim ◽  
Gi Ahn Jung ◽  
Dominic J. Glover ◽  
Douglas S. Clark
Keyword(s):  
Enzyme Activity ◽  
Self Assembling

scholarly journals Optimization of enzyme immobilization on magnetic microparticles using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) as a crosslinking agent

2015 ◽  
Vol 7 (24) ◽  
pp. 10291-10298 ◽  
Author(s):  
F. Kazenwadel ◽  
H. Wagner ◽  
B. E. Rapp ◽  
M. Franzreb
Keyword(s):  
Enzyme Activity ◽  
Enzyme Immobilization ◽  
Crosslinking Agent ◽  
Magnetic Microparticles ◽  
Versatile Tool

Enzyme immobilization is a versatile tool in biotransformation processes to enhance enzyme activity and to secure an easy separation of catalysts and products and the reusability of enzymes.

scholarly journals Self-assembling enzyme immobilization onto E. coli curli nanofibers for catalytic biofilms

2016 ◽  
Vol 4 ◽  
Author(s):  
Nussbaumer Martin ◽  
Botyanszki Zsofia ◽  
Tay Pei Kun ◽  
Nguyen Peter ◽  
Joshi Neel
Keyword(s):  
Enzyme Immobilization ◽  
E Coli ◽  
Self Assembling

Effect of linear charge density of polysaccharides on interactions with α-amylase: Self-Assembling behavior and application in enzyme immobilization

2020 ◽  
Vol 331 ◽  
pp. 127320 ◽  
Author(s):  
Weiping Jin ◽  
Zhifeng Wang ◽  
Dengfeng Peng ◽  
Wangyang Shen ◽  
Zhenzhou Zhu ◽  
...  
Keyword(s):  
Charge Density ◽  
Enzyme Immobilization ◽  
Linear Charge ◽  
Linear Charge Density ◽  
Self Assembling

scholarly journals Rapid protein immobilization for thin film continuous flow biocatalysis

2016 ◽  
Vol 52 (66) ◽  
pp. 10159-10162 ◽  
Author(s):  
Joshua Britton ◽  
Colin L. Raston ◽  
Gregory A. Weiss
Keyword(s):  
Thin Film ◽  
Enzyme Activity ◽  
Enzyme Immobilization ◽  
Continuous Flow ◽  
Immobilized Enzyme ◽  
Protein Immobilization

Continuous flow biocatalysis gets a new spin. An efficient and general enzyme immobilization technique for vortex fluidic processing has been developed. The immobilized enzyme demonstrated no decrease in enzyme activity over 10 h in continuous flow with a >95% reduction in quantities of required reagents and enzymes.

scholarly journals Recent Advances of Macromolecular Hydrogels for Enzyme Immobilization in the Food Products

2021 ◽  
Author(s):  
Leila Yavari Maroufi ◽  
Mohsen Rashidi ◽  
Mahnaz Tabibiazar ◽  
Maryam Mohammadi ◽  
Akram Pezeshki ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Glucose Oxidase ◽  
Enzyme Immobilization ◽  
Food Industry ◽  
High Water ◽  
Special Importance ◽  
Bioactive Substances ◽  
Arabic Gum ◽  
Macromolecular Network ◽  
Kappa Carrageenan

Enzymes are one of the main biocatalysts with various applications in the food industry. Stabilization of enzymes on insoluble carriers is important due to the low reuse, low operational stability, and high cost in applications. The immobility and the type of carrier affect the activity of the immobile enzyme. Hydrogels are three-dimensionally cross-linked macromolecular network structures designed from various polymers. Hydrogels can provide a matrix for an immobile enzyme due to their extraordinary properties such as high water absorbing capacity, carrier of bioactive substances and enzymes, biocompatibility, safety, and biodegradability. Therefore, this study mainly focuses on some enzymes (Lactase, Lipases, Amylases, Pectinase, Protease, Glucose oxidase) that are of special importance in the food industry. These enzymes could be immobilized in the hydrogels constructed of macromolecules such as kappa-carrageenan, chitosan, arabic gum, pectin, alginate, and cellulose. At last, in the preparation of these hydrogels, different enzyme immobilization methods in macromolecular hydrogels, and effect of hydrogels on enzyme activity were discussed.

Polydopamine-mediated synthesis of core–shell gold@calcium phosphate nanoparticles for enzyme immobilization

2019 ◽  
Vol 7 (7) ◽  
pp. 2841-2849 ◽  
Author(s):  
Di Li ◽  
Zheng Fang ◽  
Hongwei Duan ◽  
Li Liang
Keyword(s):  
Enzyme Activity ◽  
Calcium Phosphate ◽  
Enzyme Immobilization ◽  
Core Shell ◽  
Calcium Phosphate Nanoparticles

Constructing calcium phosphate (CaP)–gold (Au) nanocomposites for enzyme immobilization to overcome the bottleneck of loss of enzyme activity upon immobilization.

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