The use of mechano growth factor to prevent cartilage degeneration in knee osteoarthritis

2017 ◽  
Vol 12 (3) ◽  
pp. 738-749 ◽  
Author(s):  
Yang Song ◽  
Kang Xu ◽  
Can Yu ◽  
Lili Dong ◽  
Peixing Chen ◽  
...  
Keyword(s):  
Growth Factor ◽  
Knee Osteoarthritis ◽  
Cartilage Degeneration ◽  
Mechano Growth Factor

Mechano growth factor-E regulates apoptosis and inflammatory responses in fibroblast-like synoviocytes of knee osteoarthritis

2015 ◽  
Vol 39 (12) ◽  
pp. 2503-2509 ◽  
Author(s):  
Haibin Li ◽  
Mingxing Lei ◽  
Can Yu ◽  
Yonggang Lv ◽  
Yang Song ◽  
...  
Keyword(s):  
Growth Factor ◽  
Knee Osteoarthritis ◽  
Inflammatory Responses ◽  
Mechano Growth Factor ◽  
Fibroblast Like Synoviocytes

MOLECULAR CONTROL OF ARTICULAR CARTILAGE DEGENERATION BY TRANSFORMING GROWTH FACTOR ALPHA

2008 ◽  
Vol 31 (4) ◽  
pp. 2
Author(s):  
Tom Appleton ◽  
Shirine Usmani ◽  
John Mort ◽  
Frank Beier
Keyword(s):  
Gene Expression ◽  
Articular Cartilage ◽  
Growth Factor ◽  
P38 Mapk ◽  
Transforming Growth Factor ◽  
Cartilage Degeneration ◽  
Signalling Pathways ◽  
Transforming Growth Factor Alpha ◽  
Factor Alpha ◽  
Articular Cartilage Degeneration

Background: Articular cartilage degeneration is a hallmark of osteoarthritis (OA). We previously identified increased expression of transforming growth factor alpha (TGF?) and chemokine (C-C motif) ligand 2 (CCL2) in articular cartilage from a rat modelof OA (1,2). We subsequently reported that TGF? signalling modified chondrocyte cytoskeletal organization, increased catabolic and decreased anabolic gene expression and suppressed Sox9. Due to other roles in chondrocytes, we hypothesized that the effects ofTGF? on chondrocytes are mediated by Rho/ROCK and MEK/ERK signaling pathways. Methods: Primary cultures of chondrocytes and articularosteochondral explants were treated with pharmacological inhibitors of MEK1/2(U0126), ROCK (Y27632), Rho (C3), p38 MAPK (SB202190) and PI3K (LY294002) to elucidate pathway involvement. Results: Using G-LISA we determined that stimulation of primary chondrocytes with TGF? activates RhoA. Reciprocally, inhibition of RhoA/ROCK but not other signalling pathways prevents modification of the actin cytoskeleton in responseto TGF?. Inhibition of MEK/ERKsignaling rescued suppression of anabolic gene expression by TGF? including SOX9 mRNA and protein levels. Inhibition of MEK/ERK, Rho/ROCK, p38 MAPK and PI3K signalling pathways differentially controlled the induction of MMP13 and TNF? gene expression. TGF? also induced expression of CCL2 specifically through MEK/ERK activation. In turn, CCL2 treatment induced the expression of MMP3 and TNF?. Finally, we assessed cartilage degradation by immunohistochemical detection of type II collagen cleavage fragments generated by MMPs. Blockade of RhoA/ROCK and MEK/ERK signalling pathways reduced the generation of type IIcollagen cleavage fragments in response to TGF? stimulation. Conclusions: Rho/ROCK signalling mediates TGF?-induced changes inchondrocyte morphology, while MEK/ERK signalling mediates the suppression ofSox9 and its target genes, and CCL2 expression. CCL2, in turn, induces the expression of MMP3 and TNF?, two potent catabolic factors known to be involved in OA. These pathways may represent strategic targets for interventional approaches to treating cartilage degeneration in osteoarthritis. References: 1. Appleton CTG et al. Arthritis Rheum 2007;56:1854-68. 2. Appleton CTG et al. Arthritis Rheum 2007; 56:3693-705.

Mechano growth factor-C24E, a potential promoting biochemical factor for ligament tissue engineering

2016 ◽  
Vol 105 ◽  
pp. 249-263 ◽  
Author(s):  
Yang Song ◽  
Can Yu ◽  
Chunli Wang ◽  
Xingshuang Ma ◽  
Kang Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Growth Factor ◽  
Mechano Growth Factor ◽  
Biochemical Factor ◽  
Ligament Tissue Engineering

scholarly journals 193 MOLECULAR CONTROL OF ARCTICULAR CARTILAGE DEGENERATION BY TRANSFORMING GROWTH FACTOR ALPHA

2008 ◽  
Vol 16 ◽  
pp. S94
Author(s):  
T. Appleton ◽  
S. Usmani ◽  
J. Mort ◽  
F. Beier
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Cartilage Degeneration ◽  
Transforming Growth Factor Alpha ◽  
Molecular Control ◽  
Factor Alpha

scholarly journals A Novel High Sensitivity Type II Collagen Blood-Based Biomarker, PRO-C2, for Assessment of Cartilage Formation

2018 ◽  
Vol 19 (11) ◽  
pp. 3485 ◽  
Author(s):  
Yunyun Luo ◽  
Yi He ◽  
Ditte Reker ◽  
Natasja Gudmann ◽  
Kim Henriksen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Growth Factor ◽  
Knee Osteoarthritis ◽  
High Sensitivity ◽  
Type Ii Collagen ◽  
Cartilage Explants ◽  
Type Ii ◽  
Serum Samples ◽  
Human Cartilage ◽  
Cartilage Formation

N-terminal propeptide of type II collagen (PIINP) is a biomarker reflecting cartilage formation. PIINP exists in two main splice variants termed as type IIA and type IIB collagen NH2-propeptide (PIIANP, PIIBNP). PIIANP has been widely recognized as a cartilage formation biomarker. However, the utility of PIIBNP as a marker in preclinical and clinical settings has not been fully investigated yet. In this study, we aimed to characterize an antibody targeting human PIIBNP and to develop an immunoassay assessing type II collagen synthesis in human blood samples. A high sensitivity electrochemiluminescence immunoassay, hsPRO-C2, was developed using a well-characterized antibody against human PIIBNP. Human cartilage explants from replaced osteoarthritis knees were cultured for ten weeks in the presence of growth factors, insulin-like growth factor 1 (IGF-1) or recombinant human fibroblast growth factor 18 (rhFGF-18). The culture medium was changed every seven days, and levels of PIIBNP, PIIANP, and matrix metalloproteinase 9-mediated degradation of type II collagen (C2M) were analyzed herein. Serum samples from a cross-sectional knee osteoarthritis cohort, as well as pediatric and rheumatoid arthritis samples, were assayed for PIIBNP and PIIANP. Western blot showed that the antibody recognized PIIBNP either as a free fragment or attached to the main molecule. Immunohistochemistry demonstrated that PIIBNP was predominately located in the extracellular matrix of the superficial and deep zones and chondrocytes in both normal and osteoarthritic articular cartilage. In addition, the hsPRO-C2 immunoassay exhibits acceptable technical performances. In the human cartilage explants model, levels of PIIBNP, but not PIIANP and C2M, were increased (2 to 7-fold) time-dependently in response to IGF-1. Moreover, there was no significant correlation between PIIBNP and PIIANP levels when measured in knee osteoarthritis, rheumatoid arthritis, and pediatric serum samples. Serum PIIBNP was significantly higher in controls (KL0/1) compared to OA groups (KL2/3/4, p = 0.012). The hsPRO-C2 assay shows completely different biological and clinical patterns than PIIANP ELISA, suggesting that it may be a promising biomarker of cartilage formation.

Mechano-growth factor induces migration of rat mesenchymal stem cells by altering its mechanical properties and activating ERK pathway

2013 ◽  
Vol 441 (1) ◽  
pp. 202-207 ◽  
Author(s):  
Jiamin Wu ◽  
Kewen Wu ◽  
Feng Lin ◽  
Qing Luo ◽  
Li Yang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Erk Pathway ◽  
Mechano Growth Factor

scholarly journals Strength Training Induced Expressiom Of Mechano-growth Factor In Human Skeletal Muscle

2004 ◽  
Vol 36 (Supplement) ◽  
pp. S52
Author(s):  
Yuefei Liu ◽  
Marcus Heinichen ◽  
Larissa Gampert ◽  
Andreas Schlumberger ◽  
Dietmar Schmidtbleicher ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Strength Training ◽  
Human Skeletal Muscle ◽  
Mechano Growth Factor

Mechano growth factor (MGF) promotes proliferation and inhibits differentiation of porcine satellite cells (PSCs) by down-regulation of key myogenic transcriptional factors

2012 ◽  
Vol 370 (1-2) ◽  
pp. 221-230 ◽  
Author(s):  
Li-Li Qin ◽  
Xiao-Kui Li ◽  
Jian Xu ◽  
De-Lin Mo ◽  
Xiong Tong ◽  
...  
Keyword(s):  
Growth Factor ◽  
Satellite Cells ◽  
Transcriptional Factors ◽  
Down Regulation ◽  
Mechano Growth Factor

scholarly journals Muscle mechano growth factor is preferentially induced by growth hormone in growth hormone-deficientlit/litmice

2004 ◽  
Vol 560 (2) ◽  
pp. 341-349 ◽  
Author(s):  
Keiji Iida ◽  
Emina Itoh ◽  
Dong-Sun Kim ◽  
Juan P. Del Rincon ◽  
Karen T. Coschigano ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Mechano Growth Factor
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