Effects of 2,4‐dichlorophenoxyacetic acid on the expression of NLRP3 inflammasome and autophagy‐related proteins as well as the protective effect of Lycium barbarum polysaccharide in neonatal rats

2021 ◽  
Author(s):  
Jian Zhou ◽  
Honghui Li ◽  
Faxuan Wang ◽  
Hengquan Wang ◽  
Ru Chai ◽  
...  
Keyword(s):  
Protective Effect ◽  
Nlrp3 Inflammasome ◽  
Dichlorophenoxyacetic Acid ◽  
Neonatal Rats ◽  
Lycium Barbarum ◽  
Lycium Barbarum Polysaccharide ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Related Proteins

On the Protective Effect of Lycium Barbarum Polysaccharide (LBP) on Optic Nerve Tissue and Retinal Ganglion Cells

2020 ◽  
Vol 10 (4) ◽  
pp. 443-448
Author(s):  
Shuzhen Wang ◽  
Zelin Xu ◽  
Yuanyuan Zhao ◽  
Chao Liu
Keyword(s):  
Optic Nerve ◽  
Optic Neuritis ◽  
Protective Effect ◽  
Intrathecal Injection ◽  
Nerve Damage ◽  
Nerve Tissue ◽  
Blue Staining ◽  
Lycium Barbarum ◽  
Optic Nerve Damage ◽  
Lycium Barbarum Polysaccharide

Background: The present paper established a model of optic neuritis injury by intrathecal injection of lipopolysaccharide (LPS) into the optic nerve, then explored the protective effect of Lycium Barbarum Polysaccharide (LBP) on optic nerve tissue and RGCs after injury. Material and methods: LPS was microinjected into the optic nerve of SD rats to induce optic neuritis. The histopathological variations of HE staining were observed in each group. The expressions of ED1/CD68 and GFAP were determined by immunofluorescence. The optic nerve repairment was evaluated by lux solid blue staining. TUNEL detects retinal cell apoptosis. Western blot was used to detect differences in expressions of related proteins. Results: LBP can alleviate the degree of optic nerve damage in rats with optic neuritis injury, inhibit the increase of LPS-induced macrophages and astrocytes in the optic nerve and retina, mitigate LPS-induced retinal RGC damage, inhibit LPS-induced expressions of proinflammatory cytokines TNF-α and IL-6, COX2 and iNOS in the optic nerve injury model of optic neuritis in rats, as well as inhibit the activation of STAT1, STAT3 and NF-κB pathways. Conclusion: LBP can ameliorate optic nerve damage, and the mechanism may be realized through inhibiting NF- B and STAT signaling pathways.

scholarly journals Lycium barbarum polysaccharide attenuates alcoholic cellular injury through TXNIP-NLRP3 inflammasome pathway

2014 ◽  
Vol 69 ◽  
pp. 73-78 ◽  
Author(s):  
Jia Xiao ◽  
Yinghui Zhu ◽  
Yingxia Liu ◽  
George L. Tipoe ◽  
Feiyue Xing ◽  
...  
Keyword(s):  
Nlrp3 Inflammasome ◽  
Cellular Injury ◽  
Lycium Barbarum ◽  
Lycium Barbarum Polysaccharide

scholarly journals Protective effect of Lycium barbarum polysaccharide on ethanol‐induced injury in human hepatocyte and its mechanism

2020 ◽  
Vol 44 (10) ◽  
Author(s):  
Jianteng Wei ◽  
Linghao Zhang ◽  
Jianfei Liu ◽  
Dong Pei ◽  
Ningli Wang ◽  
...  
Keyword(s):  
Protective Effect ◽  
Human Hepatocyte ◽  
Lycium Barbarum ◽  
Lycium Barbarum Polysaccharide

Protective effect of Lycium barbarum polysaccharide 4 on kidneys in streptozotocin-induced diabetic rats

2009 ◽  
Vol 87 (9) ◽  
pp. 711-719 ◽  
Author(s):  
Rui Zhao ◽  
Qing-wang Li ◽  
Jian Li ◽  
Tao Zhang
Keyword(s):  
Protective Effect ◽  
Mesangial Cells ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Protective Mechanism ◽  
Lycium Barbarum ◽  
Physiological Level ◽  
Extracellular Signal ◽  
Lycium Barbarum Polysaccharide

Lycium barbarum polysaccharide (LBP) has been shown to have hypoglycemic and antioxidative properties, although its mode of action is yet unknown. Because oxidative stress is implicated in the pathogenesis of diabetic nephropathy, we evaluated the protective effect of LBP-4, the major active component of Lycium barbarum, on the defensive antioxidative mechanism in kidneys in a streptozotocin-induced diabetic rat model. Moreover, we investigated the effects of LBP-4 on the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) in isolated mesangial cells. The role of protein kinase C (PKC)-dependent and -independent pathways in LBP-4-reduced ERK1/2 was studied by bisindolylmaleimide (BIM) IV, an inhibitor of PKC. Diabetic rats treated with LBP-4 (10 mg/kg) for 8 weeks showed increased activity of antioxidant enzymes and increased scavenging of oxygen radicals, while the activity of PKC in the renal cortex was maintained at a physiological level. The decreased activation of ERK1/2 in mesangial cells, through the involvement of PKC, could explain the protective mechanism in kidneys of diabetic rats treated with LBP-4.

In Vitro Shoot Regeneration from Callus Derived from Marubakaido Apple Rootstock under Different Aluminium Concentrations

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460e-460 ◽  
Author(s):  
Marisa F. de Oliveira ◽  
Gerson R. de L. Fortes ◽  
João B. da Silva
Keyword(s):  
Shoot Regeneration ◽  
Dichlorophenoxyacetic Acid ◽  
Apple Rootstock ◽  
Under Five ◽  
Significant Difference ◽  
Previously Treated ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.

Characterization of the Reproductive Mode in Guayule In Vitro

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 483a-483
Author(s):  
Roy N. Keys ◽  
Dennis T. Ray ◽  
David A. Dierig
Keyword(s):  
Field Experiments ◽  
Reproductive Mode ◽  
Dichlorophenoxyacetic Acid ◽  
Initial Field ◽  
Breeding Lines ◽  
Reproductive Modes ◽  
Desert Southwest ◽  
2,4 Dichlorophenoxyacetic Acid

Guayule (Parthenium argentatum Gray, Asteraceae) is a latex-producing perennial desert shrub that is potentially of economic importance as an industrial crop for the desert Southwest. It is known to possess complex reproductive modes. Diploids are predominantly sexual and self-incompatible, while polyploids show a range of apomictic potential and self-compatibility. This paper describes the development of a relatively rapid and simple technique for characterizing reproductive modes of breeding lines of P. argentatum. Initial field experiments were based on an auxin test used successfully to characterize reproductive mode in the Poaceae. The application of 2,4-dichlorophenoxyacetic acid inhibited embryo formation in P. argentatum, but this was not the case with other auxins tested. Results of field experiments were ambiguous because: 1) the floral structure of P. argentatum is such that auxins might not have penetrated to the ovules, and 2) there was potential self-fertilization by pollen released within isolation bags. Therefore, in vitro culture of flower heads was tested because it provided much better control of environmental conditions, growth regulator application, and pollen release. Auxin alone, or in combination with gibberellic acid or kinetin, inhibited parthenogenesis in vitro. Embryo production did not vary using two substantially different nutrient media. In vitro flower head culture using a (Nitsch and Nitsch) liquid nutrient medium without growth regulators, enabled characterization of the reproductive mode of seven breeding lines, ranging from predominantly sexual to predominantly apomictic. The results of this technique were substantiated using RAPD analyzes of progeny arrays from controlled crosses.

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