Protective effect of Thymus munbyanus aqueous extract against 2,4-dichlorophenoxyacetic acid-induced nephrotoxicity in Wistar rats

Abstract Background Hepatorenal injuries and dyslipidemia are common global health challenges but medicinal plant extracts may have potential to prevent them. Thus, this study evaluated the protective effect of aqueous extract of Lophira lanceolata leaf (LLE) against cisplatin-induced hepatorenal injuries and dyslipidemia in albino Wistar rats. Methods Thirty rats were randomly divided into 6 groups of 5 rats each. Group I rats received distilled water and served as control, group II rats were given 5 mg/kg cisplatin (CIS) intraperitoneally, groups III and IV rats were treated with 200 and 400 mg/kg LLE respectively for 26 days by oral gavages while groups V and VI rats were treated with 200 and 400 mg/kg LLE respectively, followed by CIS on the 21st day as in group II. About 24 h after treatment, blood was collected from the rats; then serum was separated and used for estimations of biochemical parameters. The kidney and liver of rats were removed, rinsed in normal saline, stored in 10% formalin and used for histological analyses. Results The biomarkers of hepatic (Aminotransferases, Alkaline phosphatase and Bilirubin) and renal (urea and creatinine) injuries, and dyslipidemia (Total cholesterol, triglycerides and LDL-cholesterol) significantly (p < 0.05) increased in the rats exclusively exposed to cisplatin when compared with normal control. However, treatment of cisplatin-exposed rats with 200 and 400 mg/kg LLE significantly (p < 0.05) reduced the levels of these biomarkers of hepatorenal injuries and dyslipidemia when compared with cisplatin control. Photomicrographs showed pathological signs in the liver and kidney of rats exclusively exposed to cisplatin, but there was moderate protection of these tissues in the rats treated with LLE and cisplatin. Conclusion The current findings have shown that Lophira lanceolata leaf extract may provide moderate protection against cisplatin-induced hepatorenal injuries and dyslipidemia in albino Wistar rats.

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The antioxidant study proprieties of Thymus munbyanus aqueous extract and its beneficial effect on 2, 4-Dichlorophenoxyacetic acid -induced hepatic oxidative stress in albino Wistar rats

Toxicology Mechanisms and Methods ◽

10.1080/15376516.2020.1870183 ◽

2020 ◽

pp. 1-29

Author(s):

Lazhari Tichati ◽

Fouzia Trea ◽

Kheireddine Ouali

Keyword(s):

Oxidative Stress ◽

Beneficial Effect ◽

Aqueous Extract ◽

Wistar Rats ◽

Dichlorophenoxyacetic Acid ◽

Hepatic Oxidative Stress

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The Effect of Aqueous Extract of Ocimum gratissium (Linn) on 1, 2 - Dimethyl Hydrazine induced Colon Cancer in Male Wistar Rats

International Journal of Human Anatomy ◽

10.14302/issn.2577-2279.ijha-20-3180 ◽

2020 ◽

Vol 2 (1) ◽

pp. 22-35

Author(s):

Ugochukwu Umeaku ◽

David A. Ofusori ◽

Obioma P. Umeaku ◽

Tolulope A. Edward

Keyword(s):

Colon Cancer ◽

Protective Effect ◽

Aqueous Extract ◽

Wistar Rats ◽

Control Group ◽

Steady Increase ◽

Colonic Wall ◽

Cancer Induction ◽

Male Wistar Rats ◽

Group B

Background Colon cancer is one of the leading causes of cancer death world-wide. There is a steady increase in incidence over the past four decades in developing countries. This has been partly attributed to increasingly low intake of vegetables among other causes. Aims Therefore this study aims to evaluate the protective effect of aqueous extract of Ocimumgratissimum (OG) leaves (a staple vegetable) on experimental model of colon carcinogenesis induced with 1, 2 Dimethylhydrazine (DMH). This is compared with celecoxib (a cyclooxygenase-2 inhibitor) which is used in the chemoprevention of colon cancer. Methods Sixty adult male Wistar rats were randomly divided into six groups: A to F, n=10. Group A was the normal control, Group B was given only DMH weekly for 16 weeks,Groups C, D and E were given graded doses of OG for two weeks prior to cancer induction by DMH. After which both OG and DMH were given for 16weeks. Group F received celecoxib daily for two weeks prior to cancer induction. Colonic wall was analysed grossly, histologically and biochemically. The induced lesions were staged investigated and staged using Duke’s Staging method. Results The result showed tumour incidence in groups B and C while no evidence of primary colonic tumour was observed in groups A, D, E and F. There was a dose dependent increase in the goblet cell count in the groups treated with OG with group E being statistically higher than group F. There was a significant reduction in collagen staining intensity (F = 129.74, p < 0.0001) for the colonic wall in group B when compared to other groups. There was a decreased nucleo-cytoplasmic ratio in groups C, D, E and F when compared to group B. There was a significant increase in the concentration of nitric oxide and prostaglandin E2in group B when compared to other groups D, E and F. Conclusion In conclusion, this research showed a protective effect of Ocimumgratissimum leaves on 1, 2-dimethylhydrazine-induced colon cancer which further corroborated its ethno-medicinal use.

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Effects of oral administration of 2,4-dichlorophenoxyacetic acid (2,4-D) on reproductive parameters in male Wistar rats

Environmental Science and Pollution Research ◽

10.1007/s11356-016-7656-3 ◽

2016 ◽

Vol 24 (1) ◽

pp. 519-526 ◽

Cited By ~ 10

Author(s):

Neila Marouani ◽

Olfa Tebourbi ◽

Donia Cherif ◽

Dorsaf Hallegue ◽

Mohamed Tahar Yacoubi ◽

...

Keyword(s):

Oral Administration ◽

Wistar Rats ◽

Dichlorophenoxyacetic Acid ◽

Reproductive Parameters ◽

Male Wistar Rats ◽

2,4 Dichlorophenoxyacetic Acid

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Allium sativum aqueous extract does not have chemo-protective effect on etoposide induced therapy-related DNA damage leading to Acute Myeloid Leukemia in albino-wistar rats

African Health Sciences ◽

10.4314/ahs.v21i2.24 ◽

2021 ◽

Vol 21 (2) ◽

pp. 673-682

Author(s):

Ugochi F Ndiokwelu ◽

Liasu A Ogunkanmi ◽

Joseph B Minari ◽

Ijeoma C Uzoma

Keyword(s):

Bone Marrow ◽

Acute Myeloid Leukemia ◽

Protective Effect ◽

Aqueous Extract ◽

Myeloid Leukemia ◽

Wistar Rats ◽

Allium Sativum ◽

Control Groups ◽

And Control ◽

Acute Myeloid

Background: Therapy-related acute myeloid leukemia (t-AML) is a well-recognized clinical syndrome occurring in a signif- icant fraction of patients who have undergone previous chemotherapy for a solid tumour. Objectives: We aim to evaluate the effect of aqueous extract of fresh Allium sativum cloves on haematological parameters, bone marrow and DNA of etoposide treated albino wistar rats. Decoction method was used to prepare plant extracts and the rats were weighed and divided into experimental and control groups. Blood and bone marrow sample were analysed and DNA fragment analysis was carried out. Results: There was progressive increase in the weight of animals that received distilled water only for the duration of the experiment while those that received etoposide only showed a sharp decrease in weight by the end of week 3. There was no significant difference in the mean of the haematological parameters in the test and control groups except for platelet count. The bone marrow smears showed no prevention of erythroblast fragmentation by the extract, in the same vein, DNA dam- age was not abated. Conclusion: Aqueous extract of fresh Allium sativum cloves may not be the option for the prevention of etoposide induced acute myeloid leukemia. Keywords: Allium sativum aqueous; chemo-protective effect; acute myeloid leukemia; albino-wistar rats.

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