Protective effect of Lycium barbarum polysaccharide on di-(2-ethylhexyl) phthalate-induced toxicity in rat liver

Background: The present paper established a model of optic neuritis injury by intrathecal injection of lipopolysaccharide (LPS) into the optic nerve, then explored the protective effect of Lycium Barbarum Polysaccharide (LBP) on optic nerve tissue and RGCs after injury. Material and methods: LPS was microinjected into the optic nerve of SD rats to induce optic neuritis. The histopathological variations of HE staining were observed in each group. The expressions of ED1/CD68 and GFAP were determined by immunofluorescence. The optic nerve repairment was evaluated by lux solid blue staining. TUNEL detects retinal cell apoptosis. Western blot was used to detect differences in expressions of related proteins. Results: LBP can alleviate the degree of optic nerve damage in rats with optic neuritis injury, inhibit the increase of LPS-induced macrophages and astrocytes in the optic nerve and retina, mitigate LPS-induced retinal RGC damage, inhibit LPS-induced expressions of proinflammatory cytokines TNF-α and IL-6, COX2 and iNOS in the optic nerve injury model of optic neuritis in rats, as well as inhibit the activation of STAT1, STAT3 and NF-κB pathways. Conclusion: LBP can ameliorate optic nerve damage, and the mechanism may be realized through inhibiting NF- B and STAT signaling pathways.

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Protective effect of Lycium barbarum polysaccharide on ethanol‐induced injury in human hepatocyte and its mechanism

Journal of Food Biochemistry ◽

10.1111/jfbc.13412 ◽

2020 ◽

Vol 44 (10) ◽

Author(s):

Jianteng Wei ◽

Linghao Zhang ◽

Jianfei Liu ◽

Dong Pei ◽

Ningli Wang ◽

...

Keyword(s):

Protective Effect ◽

Human Hepatocyte ◽

Lycium Barbarum ◽

Lycium Barbarum Polysaccharide

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Protective effect of Lycium barbarum polysaccharide 4 on kidneys in streptozotocin-induced diabetic rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y09-068 ◽

2009 ◽

Vol 87 (9) ◽

pp. 711-719 ◽

Cited By ~ 30

Author(s):

Rui Zhao ◽

Qing-wang Li ◽

Jian Li ◽

Tao Zhang

Keyword(s):

Protective Effect ◽

Mesangial Cells ◽

Diabetic Rats ◽

Diabetic Rat ◽

Protective Mechanism ◽

Lycium Barbarum ◽

Physiological Level ◽

Extracellular Signal ◽

Lycium Barbarum Polysaccharide

Lycium barbarum polysaccharide (LBP) has been shown to have hypoglycemic and antioxidative properties, although its mode of action is yet unknown. Because oxidative stress is implicated in the pathogenesis of diabetic nephropathy, we evaluated the protective effect of LBP-4, the major active component of Lycium barbarum, on the defensive antioxidative mechanism in kidneys in a streptozotocin-induced diabetic rat model. Moreover, we investigated the effects of LBP-4 on the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) in isolated mesangial cells. The role of protein kinase C (PKC)-dependent and -independent pathways in LBP-4-reduced ERK1/2 was studied by bisindolylmaleimide (BIM) IV, an inhibitor of PKC. Diabetic rats treated with LBP-4 (10 mg/kg) for 8 weeks showed increased activity of antioxidant enzymes and increased scavenging of oxygen radicals, while the activity of PKC in the renal cortex was maintained at a physiological level. The decreased activation of ERK1/2 in mesangial cells, through the involvement of PKC, could explain the protective mechanism in kidneys of diabetic rats treated with LBP-4.

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