Chromosome VIII disomy influences the nonsense suppression efficiency and transition metal tolerance of the yeastSaccharomyces cerevisiae

Yeast ◽  
2015 ◽  
Vol 32 (6) ◽  
pp. 479-497 ◽  
Author(s):  
S. P. Zadorsky ◽  
Y. V. Sopova ◽  
D. Y. Andreichuk ◽  
V. A. Startsev ◽  
V. P. Medvedeva ◽  
...  
Keyword(s):  
Transition Metal ◽  
Metal Tolerance ◽  
Nonsense Suppression ◽  
Suppression Efficiency ◽  
Chromosome Viii

scholarly journals The HAL3-PPZ1 dependent regulation of nonsense suppression efficiency in yeast and its influence on manifestation of the yeast prion-like determinant [ISP+]

2007 ◽  
Vol 12 (4) ◽  
pp. 435-445 ◽  
Author(s):  
Anna Aksenova ◽  
Iván Muñoz ◽  
Kirill Volkov ◽  
Joaquín Ariño ◽  
Ludmila Mironova
Keyword(s):  
Nonsense Suppression ◽  
Yeast Prion ◽  
Suppression Efficiency

scholarly journals A Transporter in the Endoplasmic Reticulum ofSchizosaccharomyces pombeCells Mediates Zinc Storage and Differentially Affects Transition Metal Tolerance

2002 ◽  
Vol 277 (20) ◽  
pp. 18215-18221 ◽  
Author(s):  
Stephan Clemens ◽  
Tanja Bloss ◽  
Christoph Vess ◽  
Dieter Neumann ◽  
Dietrich H. Nies ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Transition Metal ◽  
Metal Tolerance ◽  
Zinc Storage

scholarly journals Glutathione and Transition-Metal Homeostasis in Escherichia coli

2008 ◽  
Vol 190 (15) ◽  
pp. 5431-5438 ◽  
Author(s):  
Kerstin Helbig ◽  
Corinna Bleuel ◽  
Gerd J. Krauss ◽  
Dietrich H. Nies
Keyword(s):  
Escherichia Coli ◽  
Transition Metal ◽  
Metal Tolerance ◽  
Metal Homeostasis ◽  
Metal Resistance ◽  
Wild Type ◽  
Strong Decrease ◽  
Trace Element Metabolism ◽  
P Type ◽  
Mutant Cells

ABSTRACT Glutathione (GSH) and its derivative phytochelatin are important binding factors in transition-metal homeostasis in many eukaryotes. Here, we demonstrate that GSH is also involved in chromate, Zn(II), Cd(II), and Cu(II) homeostasis and resistance in Escherichia coli. While the loss of the ability to synthesize GSH influenced metal tolerance in wild-type cells only slightly, GSH was important for residual metal resistance in cells without metal efflux systems. In mutant cells without the P-type ATPase ZntA, the additional deletion of the GSH biosynthesis system led to a strong decrease in resistance to Cd(II) and Zn(II). Likewise, in mutant cells without the P-type ATPase CopA, the removal of GSH led to a strong decrease of Cu(II) resistance. The precursor of GSH, γ-glutamylcysteine (γEC), was not able to compensate for a lack of GSH. On the contrary, γEC-containing cells were less copper and cadmium tolerant than cells that contained neither γEC nor GSH. Thus, GSH may play an important role in trace-element metabolism not only in higher organisms but also in bacteria.

Vesicular localization of the rat ATP-binding cassette half-transporter rAbcb6

2008 ◽  
Vol 294 (2) ◽  
pp. C579-C590 ◽  
Author(s):  
Youssef Abdul Jalil ◽  
Vera Ritz ◽  
Ana Jakimenko ◽  
Christoph Schmitz-Salue ◽  
Heike Siebert ◽  
...  
Keyword(s):  
Transition Metal ◽  
Fluorescent Protein ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Subcellular Fractionation ◽  
Atp Binding ◽  
Lovo Cells ◽  
Atp Binding Cassette

The clarification of subcellular localization represents an important basis toward characterization of ATP-binding cassette (ABC) transporters and resolution of their roles in cellular physiology. Rat Abcb6 (rAbcb6) is a membrane-situated half-transporter belonging to the ABC protein superfamily. To investigate rAbcb6 subcellular distribution, the human colon adenocarcinoma line LoVo, which we found to be devoid of endogenous human ABCB6 mRNA, was employed for heterologous expression of rAbcb6 bearing a COOH-terminal epitope tag (rAbcb6-V5). Following subcellular fractionation, rAbcb6-V5 was observed as an N-glycosylated protein in fractions enriched with lysosomal/endosomal membrane proteins. Indirect immunofluorescence analyses of rAbcb6-V5 using antibodies against a rAbcb6-specific peptide or against the V5-tag revealed a punctate pattern that was colocalized with lysosome-associated membrane protein 1 (LAMP1), a marker of lysosomes/late endosomes. Substantial colocalization of tagged rAbcb6 with lysosomal/late endosomal marker was confirmed with living, unfixed LoVo cells coexpressing rAbcb6 fused to enhanced green fluorescent protein. Vesicular distribution in LoVo cells was consistent with localization of endogenous rAbcb6 expressed in rat primary hepatocyte cultures or in liver sections, as revealed by overlap of rat Lamp1 with rAbcb6 in double immunofluorescence analyses. Since several Abcb6-related half-transporters confer heavy metal tolerance, we investigated whether rAbcb6 expression in LoVo cells might affect sensitivity toward transition metal toxicity. Applying MTT viability assays, we found that expression of either rAbcb6-V5 or untagged rAbcb6 conferred tolerance toward copper, but not to cobalt or zinc. In summary, these results demonstrate that rAbcb6 is a glycosylated protein targeted to intracellular vesicular membranes and suggest involvement of rAbcb6 in transition metal homeostasis.

scholarly journals Modification of the suppressor phenotype of thymine requiring strains ofEscherichia coli

1991 ◽  
Vol 58 (3) ◽  
pp. 185-192 ◽  
Author(s):  
Muriel B. Herrington ◽  
Johnny Basso ◽  
Maria Faraci ◽  
Chantal Autexier
Keyword(s):  
Escherichia Coli ◽  
Strain Difference ◽  
Ofescherichia Coli ◽  
Nonsense Suppression ◽  
Frameshift Mutations ◽  
Novel Gene ◽  
Suppression Efficiency

SummaryThymine requiring strains ofEscherichia colisuppress nonsense and frameshift mutations during translation. Strains with different genetic backgrounds exhibited different nonsense suppression spectra and showed differences in the apparent suppression efficiency. Part of this strain difference is due to a presumably novel gene (tsmA) mapping near 39 min. This gene affects the spectrum and apparent efficiency of suppression, and appears to affect the utilization of thymidine.

High density array screening to identify the genetic requirements for transition metal tolerance in Saccharomyces cerevisiae

Metallomics ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 195 ◽  
Author(s):  
Mark R. Bleackley ◽  
Barry P. Young ◽  
Christopher J. R. Loewen ◽  
Ross T. A. MacGillivray
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transition Metal ◽  
Metal Tolerance ◽  
High Density

HREM Study of electron-induced surface radiation damage in ReO3

1991 ◽  
Vol 49 ◽  
pp. 636-637
Author(s):  
R. Ai ◽  
H.-J. Fan ◽  
L. D. Marks
Keyword(s):  
Transition Metal ◽  
Metal Ions ◽  
Metal Oxides ◽  
Electron Irradiation ◽  
Transition Metal Oxides ◽  
Carbon Film ◽  
Transition Metal Ions ◽  
Surface Radiation ◽  
Valence Transition ◽  
Electron Microscopes

It has been known for a long time that electron irradiation induces damage in maximal valence transition metal oxides such as TiO2, V2O5, and WO3, of which transition metal ions have an empty d-shell. This type of damage is excited by electronic transition and can be explained by the Knoteck-Feibelman mechanism (K-F mechanism). Although the K-F mechanism predicts that no damage should occur in transition metal oxides of which the transition metal ions have a partially filled d-shell, namely submaximal valence transition metal oxides, our recent study on ReO3 shows that submaximal valence transition metal oxides undergo damage during electron irradiation.ReO3 has a nearly cubic structure and contains a single unit in its cell: a = 3.73 Å, and α = 89°34'. TEM specimens were prepared by depositing dry powders onto a holey carbon film supported on a copper grid. Specimens were examined in Hitachi H-9000 and UHV H-9000 electron microscopes both operated at 300 keV accelerating voltage. The electron beam flux was maintained at about 10 A/cm2 during the observation.

Problems with oxygen analysis in the system MgO-Al2O3-SiO2 with the electron microprobe

1992 ◽  
Vol 50 (2) ◽  
pp. 1624-1625
Author(s):  
Michel Fialin ◽  
Guy Rémond
Keyword(s):  
Transition Metal ◽  
Metal Oxides ◽  
Transition Metal Oxides ◽  
Electrostatic Charge ◽  
Carbon Layer ◽  
Sample Holder ◽  
Rock Matrix ◽  
Electrical Discharges ◽  
Thin Carbon ◽  
Beam Instabilities

Oxygen-bearing minerals are generally strong insulators (e.g. silicates), or if not (e.g. transition metal oxides), they are included within a rock matrix which electrically isolates them from the sample holder contacts. In this respect, a thin carbon layer (150 Å in our laboratory) is evaporated on the sections in order to restore the conductivity. For silicates, overestimated oxygen concentrations are usually noted when transition metal oxides are used as standards. These trends corroborate the results of Bastin and Heijligers on MgO, Al2O3 and SiO2. According to our experiments, these errors are independent of the accelerating voltage used (fig.l).Owing to the low density of preexisting defects within the Al2O3 single-crystal, no significant charge buildup occurs under irradiation at low accelerating voltage (< 10keV). As a consequence, neither beam instabilities, due to electrical discharges within the excited volume, nor losses of energy for beam electrons before striking the sample, due to the presence of the electrostatic charge-induced potential, are noted : measurements from both coated and uncoated samples give comparable results which demonstrates that the carbon coating is not the cause of the observed errors.

Electron energy loss near edge structures of intermetallic alloys and grain boundaries in NiAl

1996 ◽  
Vol 54 ◽  
pp. 522-523
Author(s):  
G.A. Botton ◽  
C.J. Humphreys
Keyword(s):  
High Temperature ◽  
Transition Metal ◽  
Energy Loss ◽  
Grain Boundaries ◽  
Industrial Applications ◽  
Edge Structure ◽  
Structural Applications ◽  
Yield Behaviour ◽  
Late Transition ◽  
Metal Aluminides

Transition metal aluminides are of great potential interest for high temperature structural applications. Although these materials exhibit good mechanical properties at high temperature, their use in industrial applications is often limited by their intrinsic room temperature brittleness. Whilst this particular yield behaviour is directly related to the defect structure, the properties of the defects (in particular the mobility of dislocations and the slip system on which these dislocations move) are ultimately determined by the electronic structure and bonding in these materials. The lack of ductility has been attributed, at least in part, to the mixed bonding character (metallic and covalent) as inferred from ab-initio calculations. In this work, we analyse energy loss spectra and discuss the features of the near edge structure in terms of the relevant electronic states in order to compare the predictions on bonding directly with spectroscopic experiments. In this process, we compare spectra of late transition metal (TM) to early TM aluminides (FeAl and TiAl) to assess whether differences in bonding can also be detected. This information is then discussed in terms of bonding changes at grain boundaries in NiAl.

A new lithium diffusion model in layered oxides based on asymmetric but reversible transition metal migration

2020 ◽  
Vol 13 (4) ◽  
pp. 1269-1278 ◽  
Author(s):  
Kyojin Ku ◽  
Byunghoon Kim ◽  
Sung-Kyun Jung ◽  
Yue Gong ◽  
Donggun Eum ◽  
...  
Keyword(s):  
Transition Metal ◽  
Diffusion Model ◽  
Layered Oxides ◽  
Layered Oxide ◽  
Reversible Transition ◽  
Lithium Diffusion ◽  
Metal Migration

We propose a new lithium diffusion model involving coupled lithium and transition metal migration, peculiarly occurring in a lithium-rich layered oxide.

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