Long Synthetic Peptides as Biologically Active Proteins: The Example of the Chemokines

Biologicals ◽  
2001 ◽  
Vol 29 (3-4) ◽  
pp. 259-263 ◽  
Author(s):  
Anne-Christine Thierry ◽  
Stéphane Pinaud ◽  
Nicolas Bigler ◽  
Geneviève Perrenoud ◽  
Bérangère Denis ◽  
...  
Keyword(s):  
Synthetic Peptides ◽  
Biologically Active

Biologically active leptin-related synthetic peptides activate STAT3 via phosphorylation of ERK1/2 and PI-3K

Peptides ◽  
2014 ◽  
Vol 57 ◽  
pp. 95-100 ◽  
Author(s):  
Hung-Yun Lin ◽  
Sheng-Huei Yang ◽  
Heng-Yuan Tang ◽  
Guei-Yun Cheng ◽  
Paul J. Davis ◽  
...  
Keyword(s):  
Synthetic Peptides ◽  
Biologically Active

Biologically active casein peptides implicated in immunomodulation

1989 ◽  
Vol 56 (3) ◽  
pp. 357-362 ◽  
Author(s):  
D. Migliore-Samour ◽  
F. Floc'h ◽  
P. Jollès
Keyword(s):  
Synthetic Peptides ◽  
Viral Infections ◽  
Milk Proteins ◽  
Biologically Active ◽  
Passive Immunity ◽  
Maternal Milk ◽  
Enzymic Digestion ◽  
Immunomodulating Peptides

SummaryMaternal milk should not only be considered as a nutrient, but also as a protecting agent against aggressions from the neonate's new environment. Breastfeeding facilitates transmission of a passive immunity by multifunctional factors which have a direct effect on the neonate's resistance to bacterial and viral infections. Among these factors are the main milk proteins, the caseins: during enzymic digestion of human and bovine caseins, immunomodulating peptides are released. Corresponding synthetic peptides stimulated in vitro phagocytic activity of murine and of human macrophages and exerted in vivo a protective effect against Klebsiella pneumoniae infection of mice. These data suggest that casein peptides may exert a stimulating function on the immune system of the newborn.

scholarly journals Biologically active synthetic peptides as probes of embryonic development: a competitive peptide inhibitor of fibronectin function inhibits gastrulation in amphibian embryos and neural crest cell migration in avian embryos.

1984 ◽  
Vol 99 (5) ◽  
pp. 1822-1830 ◽  
Author(s):  
J C Boucaut ◽  
T Darribère ◽  
T J Poole ◽  
H Aoyama ◽  
K M Yamada ◽  
...  
Keyword(s):  
Cell Migration ◽  
Neural Crest ◽  
Synthetic Peptides ◽  
Neural Crest Cell ◽  
Extracellular Proteins ◽  
Biologically Active ◽  
Recognition Sequence ◽  
Neural Crest Cell Migration ◽  
Crest Cell

We describe a new method for analyzing embryonic events dependent on a specific peptide recognition signal. A short, specific amino acid sequence in fibronectin has been implicated as a recognition site in fibronectin-mediated interactions. Fibroblast adhesion to fibronectin is competitively inhibited by certain synthetic peptides, including the decapeptide Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro, which appears to contain the cell recognition sequence. We found that this peptide inhibited both amphibian gastrulation and avian neural crest cell migration in vivo, as well as the attachment and migration of neural crest cells in vitro. These processes are major cell migratory events previously suggested to involve fibronectin. Negative controls included another conserved fibronectin peptide from the collagen-binding region containing the sequence Cys-Gln-Asp-Ser-Glu-Thr-Arg-Thr-Phe-Tyr and another peptide. Our results demonstrate the feasibility of using synthetic peptides directed at recognition sites in extracellular proteins as probes of morphogenetic processes, and they provide further support for the hypothesis that fibronectin is involved in gastrulation and neural crest cell migration.

scholarly journals Anti-Infective Antibody-Derived Peptides Active against Endogenous and Exogenous Fungi

2021 ◽  
Vol 9 (1) ◽  
pp. 143
Author(s):  
Tecla Ciociola ◽  
Laura Giovati ◽  
Stefania Conti ◽  
Walter Magliani
Keyword(s):  
Synthetic Peptides ◽  
Fungal Pathogens ◽  
Antifungal Agents ◽  
Opportunistic Infections ◽  
Mortality Rates ◽  
Mechanisms Of Action ◽  
Biologically Active ◽  
Malassezia Species ◽  
Etiologic Agents ◽  
Encoding Genes

Mycoses still represent relevant opportunistic infections worldwide, although overshadowed in recent years by other severe and more widespread infections. Moreover, deep-seated mycoses are often accompanied by unacceptably high mortality rates. Etiologic agents include endogenous components of the mycobiota, Candida and Malassezia species above all, and exogenous species, both yeasts and filamentous fungi. Old and new fungal pathogens are increasingly characterized by resistance to the existing antifungal agents, making imperative the search for effective and safe new therapeutics. Among the candidate molecules proposed in recent decades, synthetic peptides derived from the complementarity determining and constant regions of diverse antibodies (Abs), as well as the translated products of Ab-encoding genes, have proved of considerable interest. Their anti-infective activities, regardless of the specificity and isotype of the originating Ab, will be briefly presented and discussed in the light of their different mechanisms of action. Intriguing suggestions on the possible function of Abs after their half-life will be presented, following the recent detection, in human serum, of an antimicrobial Ab-derived peptide. Overall, Abs could represent a source of biologically active, highly flexible peptides, devoid of detectable toxicity, which can be easily synthesized and manipulated to be used, alone or in association with already available drugs, for new anti-infective strategies.

scholarly journals Modification of Alginates to Modulate Their Physic-Chemical Properties and Obtain Biomaterials with Different Functional Properties

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7264
Author(s):  
Piotr Rosiak ◽  
Ilona Latanska ◽  
Paulina Paul ◽  
Witold Sujka ◽  
Beata Kolesinska
Keyword(s):  
Drug Delivery ◽  
Regenerative Medicine ◽  
Literature Review ◽  
Functional Properties ◽  
Synthetic Peptides ◽  
Chemical Properties ◽  
Biological Properties ◽  
Biologically Active ◽  
Reaction Conditions ◽  
Wide Range

Modified alginates have a wide range of applications, including in the manufacture of dressings and scaffolds used for regenerative medicine, in systems for selective drug delivery, and as hydrogel materials. This literature review discusses the methods used to modify alginates and obtain materials with new or improved functional properties. It discusses the diverse biological and functional activity of alginates. It presents methods of modification that utilize both natural and synthetic peptides, and describes their influence on the biological properties of the alginates. The success of functionalization depends on the reaction conditions being sufficient to guarantee the desired transformations and provide modified alginates with new desirable properties, but mild enough to prevent degradation of the alginates. This review is a literature description of efficient methods of alginate functionalization using biologically active ligands. Particular attention was paid to methods of alginate functionalization with peptides, because the combination of the properties of alginates and peptides leads to the obtaining of conjugates with properties resulting from both components as well as a completely new, different functionality.

Synthetic peptides containing YIGSR and SIKVAV from laminin are biologically active in vitro and in vivo

Peptides 1992 ◽  
1993 ◽  
pp. 159-160
Author(s):  
Hynda K. Kleinman ◽  
George R. Martin ◽  
Yoshihiko Yamada
Keyword(s):  
Synthetic Peptides ◽  
Biologically Active

Plant natriuretic peptide active site determination and effects on cGMP and cell volume regulation

2007 ◽  
Vol 34 (7) ◽  
pp. 645 ◽  
Author(s):  
Yu Hua Wang ◽  
Chris Gehring ◽  
David M. Cahill ◽  
Helen R. Irving
Keyword(s):  
Cell Volume ◽  
Natriuretic Peptides ◽  
Volume Regulation ◽  
Synthetic Peptides ◽  
Stomatal Closure ◽  
Cell Volume Regulation ◽  
Mesophyll Protoplast ◽  
Biologically Active ◽  
Homologous Region ◽  
Intracellular Changes

Natriuretic peptides (NP) were first identified in animals where they play a role in the regulation of salt and water balance. This regulation is partly mediated by intracellular changes in cyclic GMP (cGMP). NP immunoanalogues occur in many plants and have been isolated, with two NP encoding genes characterised in Arabidopsis thaliana L. (AtPNP-A and AtPNP-B). Part of AtPNP-A contains the region with homology to human atrial (A)NP. We report here on the effects of recombinant AtPNP-A and smaller synthetic peptides within the ANP-homologous region with a view to identifying the biologically active domain of the molecule. Furthermore, we investigated interactions between AtPNP-A and the hormone, abscisic acid (ABA). ABA does not significantly affect Arabidopsis mesophyll protoplast volume regulation, whereas AtPNP-A and synthetic peptides promote water uptake into the protoplasts causing swelling. This effect is promoted by the membrane permeable cGMP analogue, 8-Br-cGMP, and inhibited by guanylate cyclase inhibitors indicating that increases in cGMP are an essential component of the plant natriuretic peptides (PNP) signalling cascade. ABA does not induce cGMP transients and does not affect AtPNP-A dependent cGMP increases, hence the two regulators differ in their second messenger signatures. Interestingly, AtPNP-A significantly delays and reduces the extent of ABA stimulated stomatal closure that is also based on cell volume regulation. We conclude that a complex interplay between observed PNP effects (stomatal opening and protoplast swelling) and ABA is likely to be cell type specific.

scholarly journals Synthetic Peptide Studies on the Severe Acute Respiratory Syndrome (SARS) Coronavirus Spike Glycoprotein: Perspective for SARS Vaccine Development

2004 ◽  
Vol 50 (6) ◽  
pp. 1036-1042 ◽  
Author(s):  
Wai-Yan Choy ◽  
Shu-Guang Lin ◽  
Paul Kay-Sheung Chan ◽  
John Siu-Lun Tam ◽  
Y M Dennis Lo ◽  
...  
Keyword(s):  
Immune Response ◽  
Severe Acute Respiratory Syndrome ◽  
Synthetic Peptide ◽  
Synthetic Peptides ◽  
Vaccine Development ◽  
Biologically Active ◽  
Spike Protein ◽  
Antibody Specificity ◽  
S Protein ◽  
Specific Antibodies

Abstract Background: The S (spike) protein of the etiologic coronavirus (CoV) agent of severe acute respiratory syndrome (SARS) plays a central role in mediating viral infection via receptor binding and membrane fusion between the virion and the host cell. We focused on using synthetic peptides for developing antibodies against SARS-CoV, which aimed to block viral invasion by eliciting an immune response specific to the native SARS-CoV S protein. Methods: Six peptide sequences corresponding to the surface regions of SARS-CoV S protein were designed and investigated by use of combined bioinformatics and structural analysis. These synthetic peptides were used to immunize both rabbits and monkeys. Antisera collected 1 week after the second immunization were analyzed by ELISA and tested for antibody specificity against SARS-CoV by immunofluorescent confocal microscopy. Results: Four of our six synthetic peptides (S2, S3, S5, and S6) elicited SARS-CoV-specific antibodies, of which S5 (residues 788–820) and S6 (residues 1002–1030) exhibited immunogenic responses similar to those found in a parallel investigation using truncated recombinant protein analogs of the SARS-CoV S protein. This suggested that our S5 and S6 peptides may represent two minimum biologically active sequences of the immunogenic regions of the SARS-CoV S protein. Conclusions: Synthetic peptides can elicit specific antibodies to SARS-CoV. The study provides insights for the future development of SARS vaccine via the synthetic-peptide-based approach.

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