Endophytic Bacteria in in planta Organopollutant Detoxification in Crops

2019 ◽  
pp. 1-50
Author(s):  
Michael Gatheru Waigi ◽  
Jian Wang ◽  
Bing Yang ◽  
Fredrick Owino Gudda ◽  
Wanting Ling ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
In Planta

scholarly journals In-planta Resistance to Panama Wilt of Banana is Modulated by Endophytic Bacteria: A Preliminary Study Supporting the Hypothesis

2019 ◽  
Vol 8 (07) ◽  
pp. 2262-2269
Author(s):  
Asish Kumar Panda ◽  
S. Nakkeeran ◽  
K. Soorianathasundaram
Keyword(s):  
Endophytic Bacteria ◽  
In Planta ◽  
Preliminary Study

scholarly journals EKSPLORASI DAN UJI VIRULENSI BAKTERI Bacillus sp. ENDOFIT JAGUNG TERHADAP PENYAKIT BUSUK PELEPAH JAGUNG

2020 ◽  
Vol 22 (2) ◽  
pp. 70-78
Author(s):  
Arum Saputri ◽  
Loekas Soesanto ◽  
Abu Umayah ◽  
Agus Sarjito
Keyword(s):  
Plant Height ◽  
Endophytic Bacteria ◽  
Block Design ◽  
Pathogenic Fungi ◽  
In Vitro Test ◽  
Bacillus Sp ◽  
In Planta ◽  
Fresh Weight ◽  
Randomized Block Design

[EXPLORATION AND VIRULENT TEST OF MAIZE ENDOPHYTE Bacillus sp. AGAINST MAIZE SHEATH BLIGHT]. Capability of endophytic bacterial, effectivity, and its effect on R. solani. and on maize seedlings growth were investigated from April 2018 to January 2019. Exploration of endophytes bacteria in maize was taken from Banyumas Regency (Sumbang, Kembaran, Baturraden) and Purbalingga Regency (Padamara, Bojongsari, Pratin). Taking plant samples using Purposive Random Sampling and Diagonal Sampling methods. Completely randomized design was used in in vitro test with 16 treatments repeated twice. Completely randomized block design was used in in planta experiment with 5 treatments repeated 5 times. The treatment consisted of control, fungiside (mankozeb), and 2 isolates of endophytes bacteria performing the best in vitro result. Variables observed included characteristics of endophytic bacteria and pathogenic fungi, inhibition diameter, incubation period, disease intensity, incidence of disease, AUDPC, plant height, leaf number, root length, plant fresh weight, canopy fresh weight, and root fresh weight. Resultshowed that the exploration obtained 15 endophytic Bacillus sp. isolates. The PD A.4 and BK A.1 isolates werw able to inhibit the growth of pathogenic fungi in-vitro by 56.93 and 51.5%, respectively. The soaking treatment using BK A1 was able to reduce disease intensity by 59.377%, and AUDPC value 34.19%. Endophytic bacteria influence plant height, plant fresh weight, canopy fresh weight, and fresh weight of roots respectively as 89.17 cm, 126.06 g, 106.67 g and 19.4 g.

scholarly journals Phyllosphere-inhabiting endophytic bacteria feature a stationary phase-like lifestyle

2021 ◽  
Author(s):  
André C Velásquez ◽  
José C Huguet-Tapia ◽  
Sheng Yang He
Keyword(s):  
Stationary Phase ◽  
Pseudomonas Syringae ◽  
Stress Responses ◽  
Endophytic Bacteria ◽  
Physiological State ◽  
Protein Translation ◽  
Transcriptomic Analysis ◽  
In Planta ◽  
Population Densities

Plants are in constant association with a variety of microbes, and although much is known about how symbiotic and pathogenic microbes interact with plants, less is known about the population dynamics, adaptive traits, and transcriptional features of endophytic commensal microbes that live inside leaves. In this study, we evaluated the long-term population and transcriptional dynamics of two bacterial microbiota endophytes and compared them to those of a commensal-simulating non-pathogenic mutant of the bacterial pathogen Pseudomonas syringae. We found that population densities of all three endophytic phyllosphere bacteria remained static over a long period of time, which was caused by a continual equilibrium between bacterial multiplication and death, as evidenced by treatment of plants with antibiotics that only targeted dividing bacteria or by in planta visualization of bacteria carrying a fluorescent division reporter. Population stasis could not be explained by a lack of resources, as Arabidopsis leaves could support population densities up to 100 times higher than the normal microbiota populations, nor was population stasis reversed by significantly quenching PAMP-triggered immunity. Long-term temporal in planta transcriptomic analysis of these three bacterial endophytes revealed up-regulation of protein translation, the generation of energy, and the response to stress, and interestingly, for the microbiota strains, the longer the bacteria remained inside plants, the greater the up-regulation of some of these processes. Further transcriptomic analysis of in planta populations of commensal-simulating Pseudomonas syringae revealed a remarkable resemblance to those of in vitro bacteria in stationary phase, a metabolically active physiological state in which the production of secondary metabolites and stress responses are induced. This study provides novel insight into how endophytic bacteria survive and thrive inside plant leaves, and reshapes our current understanding of what it means to be part of the endophytic microbiota in the phyllosphere.

scholarly journals SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH

2013 ◽  
Vol 13 (2) ◽  
pp. 167-178
Author(s):  
Zurai Resti ◽  
Trimurti Habazar ◽  
Deddi Prima Putra ◽  
Nasrun Nasrun
Keyword(s):  
Bacillus Cereus ◽  
Endophytic Bacteria ◽  
Disease Incidence ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Bacillus Sp ◽  
In Planta ◽  
16S Rrna Sequence ◽  
Randomized Design ◽  
Blight Disease

Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012.   Laboratory experiment consisted of three steps:  (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates.  Treatments of  in planta test were arranged in Completely Randomized Design.  Collected isolates were tested for their capability in controlling bacterial leaf blight disease on shallot.  The variables observed were disease incidence, disease severity, and shallot yield. The results showed that out of 82 isolates successfully isolated, 56 isolates (68.29%) were Gram positive, and 26 isolate (31.71%) were Gram negative. All isolates were HR negative and pathogenicity negative. Six endophytic isolates showed  better performance in inducing resistance and increasing onion yield. Based on 16S rRNA sequence the six isolates were  Bacillus cereus strain P14 , Bacillus cereus strain Se07, Bacillus sp H1, Bacillus sp SJ1 and Serratia marcescens strain PPM4.

scholarly journals CHARACTERIZATION OF ENDOPHYTIC BACILLUS ISOLATED FROM SHALLOT ROOT AS BIOCONTROL OF BACTERIAL LEAF BLIGHT DISEASE

2018 ◽  
Vol 18 (1) ◽  
pp. 31
Author(s):  
Zurai Resti ◽  
Trimurti Habazar ◽  
Deddi Prima Putra ◽  
Nasrun .
Keyword(s):  
Salicylic Acid ◽  
Endophytic Bacteria ◽  
Root Colonization ◽  
Linear Regression Analysis ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Bacillus Sp ◽  
In Planta ◽  
Blight Disease

Characterization of Endophytic Bacillus Isolated from Shallot Root as Biocontrol of Bacterial Leaf Blight Disease. Endophytic Bacillus isolated from the roots of healthy shallots has potential as biological control agent of bacterial leaf blight (BLB) disease. Based on the in planta screening, four endophytic bacteria that capable of controlling BLB diseases from the group of Bacillus were obtained, such as Bacillus cereus P14, Bacillus cereus Se07, Bacillus sp. HI, and Bacillus sp. SJI. The aims of this study were to investigate the characteristics of endophytic Bacillus that capable of controlling BLB disease and to determine the characteristic that most responsible in the disease control. This study method was descriptive. The characteristics observed were production of antibiotics by paper disc method, production of salicylic acid by capillary electrophoresis, and root colonization by in planta method. Linear regression analysis was used to determine the characteristic most responsible in the disease control. The results showed that four endophytic Bacillus were capable of producing salicylic acid and colonizing the root, and three of them were also capable of producing antibiotic. Salicylic acid production varied ranging from 13.96 to 14.72 ppm mL-1. Three endophytic Bacillus were capable of producing antibiotic with inhibition zone of 16.25 to 20.25 mm. Endophytic Bacillus was able to colonize the shallot roots with a population of bacteria ranged from 3.20 × 105-6, 20 × 105 CFU g-1 root. Based on the correlation coefficient of linear regression analysis, the root colonization of endophytic bacteria played the role in decreasing BLB disease.

In Planta Screening of Maize Seed Endophytic Bacteria for Potential Applications under Dryland Conditions

2019 ◽  
Vol 34 (1) ◽  
pp. 53 ◽  
Author(s):  
Shrey Bodhankar ◽  
Minakshi Grover ◽  
Gopal Reddy
Keyword(s):  
Endophytic Bacteria ◽  
In Planta ◽  
Maize Seed ◽  
Potential Applications

scholarly journals Karakterisasi biokimia bakteri endofit akar terung sebagai pemacu pertumbuhan tanaman dan pengendali penyakit layu bakteri in planta

2020 ◽  
Vol 1 (1) ◽  
pp. 1 ◽  
Author(s):  
Larasati Puspita Saridewi ◽  
Nur Prihatiningsih ◽  
Heru Adi Djatmiko
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Plant Protection ◽  
Block Design ◽  
Endophytic Bacterium ◽  
In Planta ◽  
Bacterial Wilt Disease ◽  
Randomized Block Design ◽  
Promote Plant Growth ◽  
Important Disease

An important disease in eggplant is bacterial wilting caused by Ralstonia solanacearum. The aim of this research is to characterize the biochemical endophytic bacteria isolated from eggplant root (BEAT) and as an agent for promoting plant growth and controlling bacterial wilt disease in planta. This research was conducted at the Plant Protection Laboratory and screen house of the Faculty of Agriculture, Jenderal Soedirman University, Purwokerto. This research was conducted descriptively to test the biochemical character of BEAT and used a Complete Randomized Block Design (RCBD) in the in planta test with 4 treatments, 6 replications and 3 plants each treatment, so that 72 plants were tested. The treatment consisted of control (without endophytic bacteria) and 3 BEAT isolates. The results showed that the endophytic bacterium AKc isolate had the ability as a bacterium to promote plant growth by producing IAA phytohormones, phosphate solvents, enzymes producing proteases, cellulase, amylase, chitinase, and HCN, as well as increasing the root volume and fresh weight of plants respectively by 40, 42% and 31%, suppressing the disease intensity by 33.33% and able to suppress bacterial wilting the best on the AUDPC value that is 47.32% -day.

scholarly journals Endophytic bacteria isolated from Leptospermum scoparium produce compounds that inhibit Ilyonectria and Neofusicoccum species in vitro

2015 ◽  
Vol 68 ◽  
pp. 445-445
Author(s):  
W.A. Wicaksono ◽  
E.E. Jones ◽  
J. Monk ◽  
H.J. Ridgway
Keyword(s):  
New Zealand ◽  
Essential Oil ◽  
International Research ◽  
Endophytic Bacteria ◽  
Antimicrobial Properties ◽  
Dual Culture ◽  
Antimicrobial Compounds ◽  
In Planta ◽  
Leptospermum Scoparium

Leptospermum scoparium JRForst et GForst var scoparium or m257;nuka is a New Zealand indigenous shrub This shrub is valued for the essential oil and medicinal honey it produces that have antimicrobial properties International research has demonstrated that endophytic bacteria can either directly produce or modify metabolites in planta Therefore bacteria within the m257;nuka endomicrobiome may also produce antimicrobial compounds A total of 192 endophytic bacteria were recovered from surface sterilised leaf stem and root tissue from three different sites These bacteria were assessed in dual culture assays against the grapevine pathogens Ilyonectria spp and Neofusicoccum spp Eleven and three endophytic bacteria showed ability to inhibit I liriodendri and N luteum respectively These endophytic bacteria produced both diffusible and volatile compounds that inhibited the pathogens Isolate W4R11 inhibited the growth of Ilyonectria spp by 4164 after 7 days incubation Isolate W1R33 could inhibit the faster growing Neofusicoccum spp by 2053 after 3 days incubation Microscopy showed that diffusible compound produced by isolate I1R21 caused hyphae and spore abnormality of Ilyonectria spp This study indicated that m257;nuka can potentially provide a new source of microorganisms for use in sustainable agriculture

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