Multiwell Plate Tools for Controlling Cellular Alignment with Grooved Topography

2014 ◽  
pp. 37-55
Author(s):  
Camila Londono ◽  
John Soleas ◽  
Petra B. Lücker ◽  
Suthamathy Sathananthan ◽  
J. Stewart Aitchison ◽  
...  
Keyword(s):  
Cellular Alignment

scholarly journals A Novel Bioreactor for the Mechanical Stimulation of Clinically Relevant Scaffolds for Muscle Tissue Engineering Purposes

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 474
Author(s):  
Silvia Todros ◽  
Silvia Spadoni ◽  
Edoardo Maghin ◽  
Martina Piccoli ◽  
Piero G. Pavan
Keyword(s):  
Mechanical Stimulation ◽  
Strain Range ◽  
Tensile Tests ◽  
Muscular Tissue ◽  
Fe Model ◽  
Mechanical Stimuli ◽  
Physiological Strain ◽  
Cellular Alignment ◽  
Stimulation Of

Muscular tissue regeneration may be enhanced in vitro by means of mechanical stimulation, inducing cellular alignment and the growth of functional fibers. In this work, a novel bioreactor is designed for the radial stimulation of porcine-derived diaphragmatic scaffolds aiming at the development of clinically relevant tissue patches. A Finite Element (FE) model of the bioreactor membrane is developed, considering two different methods for gripping muscular tissue patch during the stimulation, i.e., suturing and clamping with pliers. Tensile tests are carried out on fresh and decellularized samples of porcine diaphragmatic tissue, and a fiber-reinforced hyperelastic constitutive model is assumed to describe the mechanical behavior of tissue patches. Numerical analyses are carried out by applying pressure to the bioreactor membrane and evaluating tissue strain during the stimulation phase. The bioreactor designed in this work allows one to mechanically stimulate tissue patches in a radial direction by uniformly applying up to 30% strain. This can be achieved by adopting pliers for tissue clamping. Contrarily, the use of sutures is not advisable, since high strain levels are reached in suturing points, exceeding the physiological strain range and possibly leading to tissue laceration. FE analysis allows the optimization of the bioreactor configuration in order to ensure an efficient transduction of mechanical stimuli while preventing tissue damage.

scholarly journals Quantifying cellular alignment on anisotropic biomaterial platforms

2013 ◽  
Vol 102 (2) ◽  
pp. 420-428 ◽  
Author(s):  
Alexander R. Nectow ◽  
Misha E. Kilmer ◽  
David L. Kaplan
Keyword(s):  
Cellular Alignment

scholarly journals In Vitro Bioactivity and Biocompatibility of Bio-Inspired Ti-6Al-4V Alloy Surfaces Modified by Combined Laser Micro/Nano Structuring

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1494
Author(s):  
Chen Li ◽  
Yong Yang ◽  
Lijun Yang ◽  
Zhen Shi ◽  
Pengfei Yang ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Osteoblastic Cell ◽  
Tree Frog ◽  
Direct Writing ◽  
Multi Scale ◽  
Nano Structures ◽  
Surface Microstructures ◽  
Topographical Cues ◽  
Cellular Alignment

The bioactivity and biocompatibility play key roles in the success of dental and orthopaedic implants. Although most commercial implant systems use various surface microstructures, the ideal multi-scale topographies capable of controlling osteointegration have not yielded conclusive results. Inspired by both the isotropic adhesion of the skin structures in tree frog toe pads and the anisotropic adhesion of the corrugated ridges on the scales of Morpho butterfly wings, composite micro/nano-structures, including the array of micro-hexagons and oriented nano-ripples on titanium alloy implants, were respectively fabricated by microsecond laser direct writing and femtosecond laser-induced periodic surface structures, to improve cell adherence, alignment and proliferation on implants. The main differences in both the bioactivity in simulated body fluid and the biocompatibility in osteoblastic cell MC3T3 proliferation were measured and analyzed among Ti-6Al-4V samples with smooth surface, micro-hexagons and composite micro/nano-structures, respectively. Of note, bioinspired micro/nano-structures displayed the best bioactivity and biocompatibility after in vitro experiments, and meanwhile, the nano-ripples were able to induce cellular alignment within the micro-hexagons. The reasons for these differences were found in the topographical cues. An innovative functionalization strategy of controlling the osteointegration on titanium alloy implants is proposed using the composite micro/nano-structures, which is meaningful in various regenerative medicine applications and implant fields.

Femtosecond laser induced densification within cell-laden hydrogels results in cellular alignment

2019 ◽  
Vol 11 (3) ◽  
pp. 035005 ◽  
Author(s):  
Zheng Xiong ◽  
Haiyan Li ◽  
Puskal Kunwar ◽  
Yin Zhu ◽  
Rafael Ramos ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Cellular Alignment

Cellular alignment of differentiated osteocytes on collagen gels and three-dimensional cell migration into gels.

2019 ◽  
Vol 2019.31 (0) ◽  
pp. 1F34
Author(s):  
Keiichi ISHIKAWA ◽  
Junko SUNAGA ◽  
Yoshitaka KAMEO ◽  
Taiji ADACHI
Keyword(s):  
Cell Migration ◽  
Three Dimensional ◽  
Collagen Gels ◽  
Cellular Alignment ◽  
Dimensional Cell

Inkjet printing of laminin gradient to investigate endothelial cellular alignment

2009 ◽  
Vol 72 (2) ◽  
pp. 230-235 ◽  
Author(s):  
Kaiyong Cai ◽  
Haide Dong ◽  
Chong Chen ◽  
Li Yang ◽  
Klaus D. Jandt ◽  
...  
Keyword(s):  
Inkjet Printing ◽  
Cellular Alignment

Probing Nonlinear Cellular Responses to Integrated Mechanical Signals Through Examining Cell Alignment

2010 ◽  
Author(s):  
Robert L. Steward ◽  
Chao-Min Cheng ◽  
Philip R. LeDuc
Keyword(s):  
Fluid Flow ◽  
Mechanical Stimulation ◽  
Cellular Response ◽  
Cell Structure ◽  
Cell Alignment ◽  
Cellular Functions ◽  
Uniaxial Stretching ◽  
Cellular Alignment ◽  
Nih 3T3 ◽  
Physical And Chemical

Cells are complex systems that continuously receive signals in a variety of forms including both physical and chemical. The ability of cells to integrate these signals and already be hard wired to have coupled responses indicates the complexity at which cells function in terms of signal integration. One of the important areas in signal response is in mechanical stimulation, which has been shown to influence many cellular functions through the cytoskeleton and most often induces various cellular alignment. Most studies generally probe the affects of mechanical stimulation on cell behaviour by one mode of mechanical stimulation, though cells in fact experience multiple modes of mechanical stimulation simultaneously. From this comes the question of how does the cell process these multiple mechanical inputs? In this study we probed the effects of uniaxial stretch and/or shear fluid flow on NIH 3T3 fibroblast behaviour, specifically cell alignment. We used fluorescence microscopy to examine the orientation of the actin cytoskeleton and observed alignment along the direction of force for both uniaxial stretching and shear fluid flow in comparison to cells exposed to both mechanical modes. The cellular response surprisingly revealed an alignment that was neither parallel nor perpendicular to the direction of force. Furthermore, the integration of these 2 modes revealed a nonlinear response to combinations of shear stress and uniaxial stretching. These intriguing results have potential implications in a variety of fields including bioengineering, mechanotransduction, and cell structure.

TAMI-21. TUMOR-ASSOCIATED MICROGLIA GUIDE GBM INFILTRATION VIA PLEXIN-B2

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi202-vi202
Author(s):  
Sangjo Kang ◽  
Anirudh Sattiraju ◽  
Yuhuan Li ◽  
Shalaka Wahane ◽  
Theo Hanna ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Close Contact ◽  
Primary Brain Tumor ◽  
Physical Interaction ◽  
Wide Field ◽  
Cell Alignment ◽  
Ecm Remodeling ◽  
Underlying Mechanisms ◽  
Cellular Alignment ◽  
The Brain

Abstract Glioblastoma (GBM) is the most common malignant primary brain tumor. The nature of invasiveness of GBM makes complete surgical resection difficult. However, how GBM cells achieve wide infiltration in the brain is poorly understood. Microglia, the resident immune cells in the brain can support GBM growth and invasion, but the underlying mechanisms remain elusive. Here, we show that microglia are activated in a wide field away from tumor boundaries, ahead of tumor cell infiltration. Invading GBM cells are in close contact with microglia, progressively aligned with one another in the direction of tumor invasion. Moreover, ECM is also aligned with the infiltrating tumor and microglia, which may serve as invasion tracks in the brain. Mechanistically, we demonstrate that microglia direct cellular alignment and ECM remodeling in the invasion tracks through an axon guidance receptor Plexin-B2. Myeloid-specific ablation of Plexin-B2 perturbs microglia and tumor cell alignment, microglia migration, ECM organization, and GBM invasiveness. Together, our data reveal a hitherto under-appreciated role of microglia in providing directional cues for GBM invasion through physical interaction and alignment of ECM and tumor cells, thus providing new insights and novel molecular targets in curbing GBM invasion.

scholarly journals Nuclear and cellular alignment of primary corneal epithelial cells on topography

2012 ◽  
Vol 101A (4) ◽  
pp. 1069-1079 ◽  
Author(s):  
Vijay Krishna Raghunathan ◽  
Clayton T. McKee ◽  
Elizabeth J. Tocce ◽  
Paul F. Nealey ◽  
Paul Russell ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Cellular Alignment

Effect of Microgrooved Surfaces on Fibroblast Cells Orientation

2006 ◽  
Author(s):  
L. Altomare ◽  
S. Fare` ◽  
L. Draghi ◽  
M. C. Tanzi
Keyword(s):  
Soft Lithography ◽  
Accurate Method ◽  
In Vitro Tests ◽  
Patterned Surfaces ◽  
Periodic Patterns ◽  
Silicon Mold ◽  
Replica Molding ◽  
Laser Profilometry ◽  
Cellular Alignment

It is well known that cells can interact with patterned surfaces; although the mechanisms are still not clear, substrata containing periodic patterns of parallel grooves can impose directional constraints on cells and affect the rate and direction of cell migration. The aim of our work is to evaluate the possibility of replicating microgrooved surfaces by soft lithography and to assess, in vitro, the influence of three different microgrooved surfaces on fibroblasts alignment. Microgrooved substrata were obtained by Replica Molding out of two different polymers: PLLATMC and ChronoFlex AL from three different masters. From each master, a silicon mold was prepared and the polymers were casted in the mold using chloroform as solvent. By SEM and laser profilometry analysis we observed a good reproducibility of the different surfaces. The obtained results indicate that Replica Molding technique is an accurate method to reproduce a wide number of micropatterned surfaces with different polymers. In vitro tests with L929 murine fibroblasts showed different degrees of cellular alignment and elongation depending on grooves width.

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