Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography

2017 ◽  
pp. 191-202
Author(s):  
S. Van Wychen ◽  
L. M. L. Laurens
Keyword(s):  
Liquid Chromatography ◽  
Acid Hydrolysis ◽  
Carbohydrate Content ◽  
Total Carbohydrate ◽  
Microalgal Biomass ◽  
Content Determination ◽  
Total Carbohydrate Content

scholarly journals Vitamin B2 content determination in liver paste by using acid and acid-enzyme hydrolysis

2007 ◽  
Vol 64 (11) ◽  
pp. 744-748
Author(s):  
Zorica Basic ◽  
Vesna Kilibarda ◽  
Ivanka Miletic
Keyword(s):  
Liquid Chromatography ◽  
Enzymatic Hydrolysis ◽  
Acid Hydrolysis ◽  
Meat Product ◽  
Enzyme Hydrolysis ◽  
Free Form ◽  
Vitamin B2 ◽  
Fluorescence Detector ◽  
Content Determination

Background/Aim. Vitamin B2 is available in foodstuff in the form of coenzyme and in free form. For its content determination a few procedures should be performed (deliberation from a complex, extraction of free and deliberated form) and detection, identification and quantification. There is a particular problem in determination of vitamin B2 in the meat products. For a determination of total vitamin B2 content in liver paste two preparation procedures are compared: acid and acid-enzymatic hydrolysis. The aim of this study thus, was to compare the effectiveness of these two different procedures for vitamin B2 content determination in liver paste. Methods. High pressure liquid chromatography (HPLC) method with fluorescence detector, as specific and adequately sensitive for the foodstuff of a complex composition with a natural vitamin content, was used for determination of vitamin B2 in liver paste. Acid hydrolysis was performed with the application 0.1 M hydrochloric acid in a pressure cooker, and enzymatic hydrolysis was performed with the 10% takadiastase on 45 ?C within four hours. Ten samples of liver paste from the supply of the Serbian Army were examined. Separation was performed on the analytical column Nucleosil 50?5 C18 with mobile phase 450 ml CH3OH + 20 ml 5 mM CH3COONH4, and detection on the fluorescent detector with the variable wave length. Both methods were validated: examining a detection limit, quantification limit, specificity (because of a possible B2 vitamin interference with reagents), linearity of a peak area and standard concentration of B2 vitamin ratio in the range from 0.05 ?g/ml to 2 ?g/ml, precision for the 0.05 ?g/ml concentration and recovery. Results. All the previously examined parameters validated both methods as specific, precise and reproductive, with a high recovery (98.5% for acid and 98.2% for acid - enzymatic hydrolysis), as well as linearity in a range that significantly superseded the expected content in the samples (r = 0.9994, and r = 0.99987). Hydrolysis procedures make a sample suitable for vitamin B2 determination. In the liver paste samples a high content of vitamin B2 was determined: 0.83 mg/100 g after acid hydrolysis, and 0.909 mg/100 g after acid-enzyme hydrolysis. There were statistically significantly higher values determined after the acid-enzyme hydrolysis (p < 0.05). Conclusion. Using acid-enzyme hydrolysis and separation instrument technique (liquid chromatography) with a fluorescent detector as detection system, statistically significantly greater vitamin B2 quantities were determined than after using acid hydrolysis procedure. Vitamin B2 content determined in ten liver paste samples was high (0.881 ? 0.936 mg/100g) indicating that this meat product is a good vitamin B2 source.

Determination of Total Carbohydrate Content in Beer Using Its Pre-column Enzymatic Cleavage and HPLC-RI

2014 ◽  
Vol 7 (8) ◽  
pp. 1677-1686 ◽  
Author(s):  
Marie Jurková ◽  
Pavel Čejka ◽  
Karel Štěrba ◽  
Jana Olšovská
Keyword(s):  
Carbohydrate Content ◽  
Enzymatic Cleavage ◽  
Total Carbohydrate ◽  
Total Carbohydrate Content

scholarly journals Seasonal Patterns of Carbohydrates in Mandarin cvs. ‘Fremont’, ‘Nova’ and ‘Robinson’ on Different Rootstocks

2013 ◽  
Vol 41 (1) ◽  
pp. 255 ◽  
Author(s):  
Ercan YILDIZ ◽  
Mustafa KAPLANKIRAN ◽  
Turan Hakan DEMIRKESER ◽  
Celil TOPLU
Keyword(s):  
Seasonal Variation ◽  
Starch Content ◽  
Late Summer ◽  
Carbohydrate Content ◽  
Poncirus Trifoliata ◽  
Soluble Carbohydrate ◽  
Total Carbohydrate ◽  
Fertilization Program ◽  
Total Carbohydrate Content ◽  
Continuous Decrease

The study was conducted at the experimental farm of Mustafa Kemal University, Dörtyol, Turkey during the 2010 and 2011 growing seasons. The aim of this study was to investigate the effect of ‘Troyer’ and ‘Carrizo’ citranges (Poncirus trifoliata Raf. × Citrus sinensis Osb. var. ‘Troyer’ and ‘Carrizo’), and common sour orange (Citrus aurantium L. var. common), rootstocks on the seasonal variation of carbohydrate content in the leaves of cvs. ‘Fremont’, ‘Nova’, and ‘Robinson’ mandarin. The seasonal variation of carbohydrate content of the three cultivars budded on different rootstocks was nearly same. Soluble carbohydrate concentration showed a continuous decrease from January to mid or late-summer, and then slowly began to increase after early autumn till winter. The sucrose was the dominant soluble carbohydrate in leaves. The seasonal evolution of starch content in leaves increased initially during January to March, and then decreased in April. The starch concentration showed a continuous decrease slowly until the mid-autumn, and then accumulation began during late-autumn and winter. The total carbohydrate content differences among the rootstocks were significant, but the content was changed among the cultivars and according to the season. The change in the total carbohydrate content of leaf tissues showed a strong similarity in cultivars budded on different rootstocks throughout the year. The total carbohydrate content reached their lowest levels in July for cv. ‘Robinson’, in August for cv. ‘Fremont’ and cv. ‘Nova’. The total carbohydrate content in leaves increased from the mid- or late-summer to winter. It is suggested that the seasonal variation of carbohydrate content in plant tissues can be considered during fertilization program in mandarin trees.

scholarly journals Seasonal and needle age-related variations in the biochemical characteristics of Pinus nigra subsp. pallasiana (Lamb.) Holmboe

2018 ◽  
Vol 64 (No. 9) ◽  
pp. 379-386 ◽  
Author(s):  
Deligöz Ayşe ◽  
Bayar Esra ◽  
Genç Musa ◽  
Karatepe Yasin ◽  
Kirdar Erol ◽  
...  
Keyword(s):  
Pinus Nigra ◽  
Carbohydrate Content ◽  
Seasonal Patterns ◽  
Age Classes ◽  
Total Carbohydrate ◽  
Needle Age ◽  
Total Carbohydrate Content ◽  
Age Related ◽  
Pigment Contents ◽  
Carbohydrate Contents

Variations in the photosynthetic pigments and total carbohydrate contents of needles of different age classes (current-year, 1-year-old, 2-year-old and 3-year-old) of Pinus nigra subsp. pallasiana (Lambert) Holmboe trees in a young natural stand were investigated during the growing season. In current-year needles, total carbohydrate content was lower during June and July when the needle growth continued than in older age classes, but it was similar to other age classes in the months of August to October. Seasonal patterns of variations in total carbohydrate content were almost similar in 1-, 2-, and 3-year-old needles. Chlorophyll and carotenoid contents increased from May to June, remained relatively constant or declined slightly during summer and autumn in 1-, 2-, and 3-year-old needles. In October, the pigment content was highest in 1-year-old needles, and lowest in 3-year-old needles. Our study indicated that total carbohydrate and pigment contents were affected by needle age classes and seasons.

Development of a sulphurous acid thiolysis-high performance liquid chromatography method for the analysis of procyanidins in pine bark products

2020 ◽  
Vol 10 (9) ◽  
pp. 1581-1587
Author(s):  
Lei Shi ◽  
Chunqi Liang ◽  
Yongzhi Qi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Method ◽  
Pine Bark ◽  
Epicatechin Gallate ◽  
Chromatography Method ◽  
Content Determination ◽  
Liquid Chromatography Method

A sulphurous acid thiolysis-HPLC method for the determination of procyanidins in pine bark products was established. The concentration of sulphurous acid is 1.2%, the concentration of benzyl mercaptan is 2%, the reaction temperature is 90 °C, and the reaction time is 60 minutes. Under these conditions, the preservative rates of catechin, epicatechin, epicatechin gallate and their benzyl sulfide derivatives were 89.7%, 86.2%, 95.4%, 63.1%, 64.6% and 73.5%, respectively. According to this study, the calculation method for the procyanidin content determination by the thiolysis-HPLC method was corrected.

scholarly journals Physiological Effects of Uniconazole, Gibberellin, and Calcium on Salt Injuries of Hibiscus syriacus and H. hamabo Seedlings

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 899F-900
Author(s):  
Moo R. Huh ◽  
Beyoung H. Kwack ◽  
Leonard P. Perry
Keyword(s):  
Atpase Activity ◽  
Chlorophyll Content ◽  
Carbohydrate Content ◽  
Physiological Effects ◽  
Total Chlorophyll ◽  
Total Carbohydrate ◽  
Total Chlorophyll Content ◽  
Total Carbohydrate Content ◽  
Carbohydrate Contents ◽  
Hibiscus Syriacus

Salt injury was induced by 5% (w/v) NaCl drenching on Hibiscus hamabo Sieb. & Zucc. and H. syriacus L. seedlings. Total chlorophyll content of H. hamabo was higher than that of H. syriacus. Uniconazole (0.0, 0.5, 1.0, and 5.0 mg·liter–1) treatment increased and 25- or 50-mg·liter–1 GA3 treatment decreased chlorophyll content of H. hamabo. Total chlorophyll content of H. syriacus was not affected by uniconazole or GA3. Total carbohydrate content of H. syriacus was more accumulated than that of H. homabo. Total carbohydrate content of H. hamabo was more decreased than that of H. syriacus by Ca (13.35 or 133.5 mM), uniconazole, or GA3 in relation to total carbohydrate contents. Protein contents of H. hamabo were higher than those of H. syriacus. Uniconazole or GA3 increased those of H. hamabo and decreased those of H. syriacus. Peroxidase activity of H. hamabo was higher than that of H. syriacus. Uniconazole decreased that of H. hamabo and increased that of H. syriacus. GA3 or Ca (13.35 mM) treatment increased that of both species. ATPase activity of H. hamabo was higher than that of H. syriacus. Uniconazole (5 mg·liter–1), GA3, or Ca decreased that of H. hamabo increased that of H. syriacus.

scholarly journals Composition analysis of selected Sri Lankan seaweeds

2019 ◽  
Vol 9 (2) ◽  
Author(s):  
M.M Jayakody ◽  
M.P.G Vanniarachchy ◽  
I. Wijesekara
Keyword(s):  
Sri Lanka ◽  
Ash Content ◽  
Carbohydrate Content ◽  
Composition Analysis ◽  
Total Carbohydrate ◽  
Ulva Fasciata ◽  
Total Carbohydrate Content ◽  
Significant Difference ◽  
Total Fat ◽  
Carbohydrate Contents

Seaweeds are a rich source of health beneficial bioactive nutraceuticals and currently they are under-utilized in Sri Lanka. In the present study, proximate analysis of seaweed varieties Chnoospora minima and Porphyra sp. obtained from Mirissa, Matara, Sri Lanka and Ulva fasciata was taken from Point Dondra Matara, Sri Lanka on June, 2018 were investigated. The moisture content, total fat content, protein content and ash content were determined according to the Official methods of Analysis by Association of Official Analytical Chemists after drying for 8h at 600 C. The results revealed that the moisture contents (%) of Chnoospora minima, Porphyra sp. and Ulva fasciata were 13.24 ± 0.20, 14.30 ± 0.14 and 18.11 ± 0.01 respectively. Total fat contents (%) of Chnoospora minima, Porphyra sp. and Ulva fasciata were 0.21 ± 0.11, 0.19 ± 0.03 and 0.28 ± 0.05 respectively. Protein contents (%) of Chnoospora minima, Porphyra sp. and Ulva fasciata were 13.70 ± 0.2, 21.14 ± 0.04 and 11.84 ± 0.1. Total ash contents (%) of Chnoospora minima, Porphyra sp. and Ulva fasciata were 17.20 ± 0.24, 5.40 ± 0.7 and 18.05 ± 0.21 respectively. Total carbohydrate content (%) was analyzed according to the Dubois method. Chnoospora minima, Porphyra sp. and Ulva fasciata showed total carbohydrate content (%) as 3.87 + 0.66, 20.59 ± 0.24 and 7.68 ± 1.16 respectively. Moreover, the sulphate content was analyzed according to the precipitate method. Chnoospora minima, Porphyra sp. and Ulva fasciata showed 1.45 ± 0.35, 2.75 ± 0.07 and 4.54 ± 0.06, sulfate contents (%) respectively. In conclusion, highest ash content which indicates a good mineral content was observed in Ulva fasciata and Chnoospora minima. Fibre, protein and carbohydrate contents are significantly different among the 3 samples. Highest fibre content was observed in Chnoospora minima. Highest protein and carbohydrate contents were observed in Porphyra sp. But there is no significant difference in fat contents among the three samples.

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