Single Cell Densitometry and Weightlessness Culture of Mesenchymal Stem Cells Using Magnetic Levitation

2019 ◽  
pp. 15-25 ◽  
Author(s):  
Muge Anil-Inevi ◽  
Esra Yilmaz ◽  
Oyku Sarigil ◽  
H. Cumhur Tekin ◽  
Engin Ozcivici
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Single Cell ◽  
Magnetic Levitation

scholarly journals Single-Cell RNA-Seq of Bone Marrow-Derived Mesenchymal Stem Cells Reveals Unique Profiles of Lineage Priming

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0136199 ◽  
Author(s):  
Brian T. Freeman ◽  
Jangwook P. Jung ◽  
Brenda M. Ogle
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Single Cell ◽  
Rna Seq ◽  
Lineage Priming

scholarly journals Dependence of Spreading and Differentiation of Mesenchymal Stem Cells on Micropatterned Surface Area

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Wei Song ◽  
Naoki Kawazoe ◽  
Guoping Chen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Single Cell ◽  
Adipogenic Differentiation ◽  
Cell Spreading ◽  
Poly Vinyl Alcohol ◽  
Cell Array ◽  
Cell Functions

Micropatterning technology is a highly advantageous approach for directly assessing and comparing the effects of different factors on stem cell functions. In this study, poly(vinyl alcohol)- (PVA-) micropatterned polystyrene surfaces were prepared using photoreactive PVA and ultraviolet photolithography with a photomask. The micropatterned surface was suitable for single-cell array formation and long-term cell culture due to the nanometer thickness of nonadhesive PVA layer. Different degrees of cell spreading with the same cell shape were established by adjusting the sizes of circular, cell-adhesive polystyrene micropatterns. Cell spreading and differentiation of mesenchymal stem cells (MSCs) on the micropatterns were investigated at the single-cell level. The assembly and organization of the cytoskeleton were regulated by the degree of cell spreading. Individual MSCs on large circular micropatterns exhibited a more highly ordered arrangement of actin filaments than did those on the small circular micropatterns. Furthermore, the differentiation of MSCs was dependent on the degree of cell spreading. Increased cell spreading facilitated the osteogenic differentiation but suppressed the adipogenic differentiation of MSCs. This micropatterning method is valuable for stem cell research in tissue engineering and regenerative medicine.

scholarly journals PB1988 CANCER-ASSOCIATED MECHANISMS OF MESENCHYMAL STEM CELLS REVEALED BY SINGLE-CELL TRANSCRIPTOME ANALYSIS

HemaSphere ◽  
2019 ◽  
Vol 3 (S1) ◽  
pp. 901
Author(s):  
A. Stucky ◽  
X. Chen ◽  
C. Li ◽  
J. cai ◽  
X. Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Single Cell ◽  
Transcriptome Analysis ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals Single cell migration profiling on a microenvironmentally tunable hydrogel microstructure device that enables stem cell potency evaluation

Lab on a Chip ◽  
2020 ◽  
Vol 20 (5) ◽  
pp. 958-972
Author(s):  
Enrique Ros ◽  
Matías Encina ◽  
Fabián González ◽  
Rafael Contreras ◽  
Patricia Luz-Crawford ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Migration ◽  
Single Cell

Detailed cell migration profiling allows for accurate correlations with therapeutic functions of mesenchymal stem cells.

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