scholarly journals Polymeric Nanoparticle-Based Vaccine Adjuvants and Delivery Vehicles

2020 ◽  
Author(s):  
Elizabeth A. Grego ◽  
Alaric C. Siddoway ◽  
Metin Uz ◽  
Luman Liu ◽  
John C. Christiansen ◽  
...  
Keyword(s):  
Vaccine Adjuvants ◽  
Polymeric Nanoparticle ◽  
Delivery Vehicles

scholarly journals Nanoparticle engineered TRAIL-overexpressing adipose-derived stem cells target and eradicate glioblastoma via intracranial delivery

2016 ◽  
Vol 113 (48) ◽  
pp. 13857-13862 ◽  
Author(s):  
Xinyi Jiang ◽  
Sergio Fitch ◽  
Christine Wang ◽  
Christy Wilson ◽  
Jianfeng Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Xenograft Model ◽  
Adipose Derived Stem Cells ◽  
Drug Delivery Vehicles ◽  
Effective Strategies ◽  
Polymeric Nanoparticle ◽  
Promising Therapeutic Approach ◽  
Delivery Vehicles ◽  
Necrosis Factor

Glioblastoma multiforme (GBM) is one of the most intractable of human cancers, principally because of the highly infiltrative nature of these neoplasms. Tracking and eradicating infiltrating GBM cells and tumor microsatellites is of utmost importance for the treatment of this devastating disease, yet effective strategies remain elusive. Here we report polymeric nanoparticle-engineered human adipose-derived stem cells (hADSCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) as drug-delivery vehicles for targeting and eradicating GBM cells in vivo. Our results showed that polymeric nanoparticle-mediated transfection led to robust up-regulation of TRAIL in hADSCs, and that TRAIL-expressing hADSCs induced tumor-specific apoptosis. When transplanted in a mouse intracranial xenograft model of patient-derived glioblastoma cells, hADSCs exhibited long-range directional migration and infiltration toward GBM tumor. Importantly, TRAIL-overexpressing hADSCs inhibited GBM growth, extended survival, and reduced the occurrence of microsatellites. Repetitive injection of TRAIL-overexpressing hADSCs significantly prolonged animal survival compared with single injection of these cells. Taken together, our data suggest that nanoparticle-engineered TRAIL-expressing hADSCs exhibit the therapeutically relevant behavior of “seek-and-destroy” tumortropic migration and could be a promising therapeutic approach to improve the treatment outcomes of patients with malignant brain tumors.

scholarly journals Applications of Chitosan-Alginate-Based Nanoparticles—An Up-to-Date Review

Nanomaterials ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 186
Author(s):  
Adelina-Gabriela Niculescu ◽  
Alexandru Mihai Grumezescu
Keyword(s):  
Food Industry ◽  
Antimicrobial Agents ◽  
Vaccine Adjuvants ◽  
Natural Polymers ◽  
Natural Origin ◽  
Environmental Friendly ◽  
Delivery Vehicles ◽  
Multiple Uses ◽  
Biomedical Field ◽  
Inception Point

Chitosan and alginate are two of the most studied natural polymers that have attracted interest for multiple uses in their nano form. The biomedical field is one of the domains benefiting the most from the development of nanotechnology, as increasing research interest has been oriented to developing chitosan-alginate biocompatible delivery vehicles, antimicrobial agents, and vaccine adjuvants. Moreover, these nanomaterials of natural origin have also become appealing for environmental protection (e.g., water treatment, environmental-friendly fertilizers, herbicides, and pesticides) and the food industry. In this respect, the present paper aims to discuss some of the newest applications of chitosan-alginate-based nanomaterials and serve as an inception point for further research in the field.

scholarly journals Combination of Innate Immune Modulators as Vaccine Adjuvants in Mice

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 569
Author(s):  
Azita Haddadi ◽  
Alyssa Chaffey ◽  
Siew Hon Ng ◽  
Damayanthi Yalamati ◽  
Heather L. Wilson
Keyword(s):  
Lipid A ◽  
Muramyl Dipeptide ◽  
Humoral Response ◽  
Innate Immune ◽  
Poly I:C ◽  
Vaccine Adjuvants ◽  
Immune Modulators ◽  
Polyinosinic:Polycytidylic Acid ◽  
Polymeric Nanoparticle ◽  
Tlr2 Ligand

The development of new, effective, and safe vaccines necessarily requires the identification of new adjuvant(s) to enhance the potency and longevity of antigen-specific immune responses. In the present study, we compare the antibody-mediated and cell-mediated immune (CMI) responses within groups of mice vaccinated subcutaneously with ovalbumin (OVA; as an experimental antigen) plus polyphosphazene (an innate immune modulator), Polyinosinic:polycytidylic acid (poly-I:C; (an RNA mimetic) and glycopeptide ARC5 (which is a Toll-like receptor (TLR), TLR2 ligand and PAM3CSK4 analogue) formulated together in a soluble vaccine. We also investigated the effect of a polymeric nanoparticle of ARC4 and ARC7 (which are a novel muramyl dipeptide analogue and a monophosophoryl lipid A (MPLA) analogue, respectively) plus OVA +/− ARC5 as a subcutaneous vaccine in mice. OVA+ARC4/ARC7 nanoparticle +/− ARC5 triggered a robust and balanced Th1/Th2-type humoral response with significant anti-OVA IgA in serum, and significant interferon (IFN)-γ and interleukin (IL)-17 production in splenocytes after 35 days relative to the controls. Formulation of OVA with ARC4/ARC7 nanoparticles should be investigated for inducing protective immunity against infectious pathogens in mice and other species.

scholarly journals ​Selecting the Best Combination of Adjuvant and the Delivery Vehicle for Optimizing the Th1 and Th2 Biased Responses of NDV Live Vaccine in Indigenous Aseel Chicken

2021 ◽  
Author(s):  
Manmeet Kaur ◽  
C.S. Mukhopadhyay ◽  
Ramneek . ◽  
R.S. Sethi ◽  
Dipak Deka ◽  
...  
Keyword(s):  
Saline Solution ◽  
Poly I:C ◽  
Cpg Odn ◽  
Vaccine Adjuvants ◽  
Delivery Vehicle ◽  
Water Emulsion ◽  
Oil In Water ◽  
Delivery Vehicles ◽  
Oil In Water Emulsion ◽  
Th1 And Th2

Background: This study aimed at the evaluation of the efficacy of TLR ligands (viz. Poly I:C, Lipopolysaccharide and CpG:ODN) as vaccine adjuvants in combination with a suitable carrier (oil-in-water emulsion, normal saline solution, or alum) by studying the expression profile of cytokines belonging to Th1 and Th2 biased immune systems. Methods: Whole blood was collected from 108 birds that were divided into 9 different experimental groups: 3 TLR ligands each with 3 delivery vehicles. Th1 (IFN-gamma) and Th2 (IL4) biased cytokines were detected by qPCR followed by ELISA. Both the results showed the highest level of IL4 (Th1 biased) with LPS and the highest level of IFNg (Th1 biased) with CpG-ODN. Uneven TLR expression was detected from day 3 onwards till day 21 but ligands and delivery vehicles administered along with NDV vaccine did not show significant influence on the immune response. Result: It is evident that the TLR-3, 4 and 9 expressions increase following exposure to the respective ligands. Hence, TLR ligands can be used as reliable adjuvants in NDV vaccine. The expression of the Th2 biased cytokine IL4 mRNA and Th1 biased cytokine Interferon-gamma was found to be the highest on day 3 in the LPS+NSS group and on day 21 in CpG-ODN+Alum group, respectively. The findings of the real-time PCR were supported to some by the ELISA.

Iono-molecular Separation with Composite Membranes II. Preparation and characterization of polysulphone and composite nanoparticles

2017 ◽  
Vol 68 (2) ◽  
pp. 203-209
Author(s):  
Hussam Nadum Abdalraheem Al Ani ◽  
Anca Maria Cimbru ◽  
Corneliu Trisca-Rusu ◽  
Szidonia Katalin Tanczos ◽  
Adriana Cuciureanu ◽  
...  
Keyword(s):  
Chemical Species ◽  
Composite Membranes ◽  
Ammonium Persulfate ◽  
Operating Pressure ◽  
Polysulfone Membrane ◽  
Polymeric Nanoparticle ◽  
Molecular Separation ◽  
Copper Complexation ◽  
Molecular Separations ◽  
Methyl Pyrrolidone

This paper illustrates the possibility of producing iono-molecular separations using ionic colloidal ultrafiltration membrane of polysulfone synthetic solutions of cupric ions and nitro phenols through ultrafiltration assisted by polymeric nanoparticle composites based on polysulfone. In the present work, in order to reduce the operating pressure and increase the flow of water we are using the process of ultrafiltration through a polysulfone membrane in N-methylpyrrolidone 10% prepared by coagulation in isopropanol. The nanoparticles needed in colloidal ultrafiltration had been obtained through the immersion technique of precipitation of a solution of 5% PSf in N-methyl pyrrolidone containing 3% aniline in lower alcohols: methanol, ethanol, and isopropanol, followed by the oxidation of the remaining aniline in a solution of 10% hydrochloric acid and ammonium persulfate. The Nanoparticles of polysulfone (NP-PSf) and The three obtained variants of nanoparticles composites (NP-PSf-PANI) were morphologically (SEM) and (AFM), structurally and compositionally (FTIR) characterized and the results show that nanoparticles polysulfone have a much lower range than the composites. The Possibility of copper complexation by both nitrophenols, and by nanoparticle surface probably lead to the formation of more stable aggregates in the supply, which can sufficiently justify the increased retention. The Retentions of the chemical species in question use in all the tests made the same series:R NP-PSf-PANI-M] R NP-PSf-PANI-E] R NP-PSf-PANI-P] R NP-PSf

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