Determination of Redox Status in Serum

2020 ◽  
pp. 115-128
Author(s):  
Kelly Casós ◽  
Cristina Costa ◽  
Manuel Galiñanes
Keyword(s):  
Redox Status

Effect of thiol oxidation and thiol export from erythrocytes on determination of redox status of homocysteine and other thiols in plasma from healthy subjects and patients with cerebral infarction

1995 ◽  
Vol 41 (3) ◽  
pp. 361-366 ◽  
Author(s):  
A Andersson ◽  
A Lindgren ◽  
B Hultberg
Keyword(s):  
Cerebral Infarction ◽  
Healthy Subjects ◽  
Redox Status ◽  
Reduced Form ◽  
Temperature Dependent ◽  
First Order ◽  
First Order Kinetics ◽  
Before And After ◽  
Reduced Forms

Abstract Changes in concentration of reduced and oxidized low-M(r) thiols were measured in blood and plasma before and after the separation of blood cells. If centrifugation of blood was postponed, the reduced form of homocysteine in plasma increased with time at 22 degrees C; in contrast, the concentrations of other reduced thiols (cysteine, glutathione, and cysteinylglycine) decreased. In plasma the reduced forms of all thiols disappeared at a rate that followed first-order kinetics. The rates of disappearance ("half-lives") were temperature-dependent; they were about the same for glutathione and homocysteine (11.7 and 14.3 min, respectively, at 22 degrees C) and somewhat higher for cysteinylglycine and cysteine. After establishing proper sampling conditions for reduced thiols, we measured this thiol fraction as well as free (non-protein-bound) and total thiols in 10 reference subjects and 19 patients with cerebral infarction. Mild but significant hyperhomocysteinemia involving total and free homocysteine (but not reduced homocysteine) was found in the patients.

Determination of the in vivo redox status of cysteine, cysteinylglycine, homocysteine, and glutathione in human plasma

1992 ◽  
Vol 200 (2) ◽  
pp. 218-229 ◽  
Author(s):  
Mohammad A. Mansoor ◽  
Asbjørn M. Svardal ◽  
Per M. Ueland
Keyword(s):  
Human Plasma ◽  
Redox Status

scholarly journals Determination of Prooxidant–Antioxidant Balance during Uncomplicated Pregnancy Using a Rapid Assay

2013 ◽  
Vol 32 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Hassan Boskabadi ◽  
Mahdieh Moeini ◽  
Fatemeh Tara ◽  
Shima Tavallaie ◽  
Hamidreza Saber ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pregnant Women ◽  
Demographic Data ◽  
First Trimester ◽  
Normal Pregnancy ◽  
Redox Status ◽  
Uncomplicated Pregnancy ◽  
Biochemical Indices ◽  
Post Hoc

Summary Background: Oxidative stress is thought to be a major contributor to complications during pregnancy, for example preeclampsia. However, reports regarding prooxidant-antioxidant balance in uncomplicated pregnancy are inconsistent. In this study, we aimed to compare the levels of oxidative stress in non-pregnant women with apparently normal pregnant women during the first trimester and at delivery. Methods: An assay for the determination of prooxidant-antioxidant balance (PAB) was used in this study, in which the prooxidant burden and the antioxidant capacity were measured simultaneously in a single assay. The levels of oxidative stress were determined in 85 non-pregnant and 64 primigravid pregnant women. Results: Demographic data and biochemical indices did not differ significantly between the groups. Differences between PAB values were significant based on one-way ANOVA analysis (P<0.001). Using a post hoc test, we observed a statistically significant increase in PAB values during the first trimester and last trimester (P<0.001). Conclusions: Normal pregnancy is associated with a change in the measure of redox status, as assessed by the PAB assay.

scholarly journals Glucose-6- Phosphate Dehydrogenase Activity in Newborn in Jos: A Necessary Evaluation for Icteric Neonates

2019 ◽  
Vol 2 (4) ◽  
pp. 191-196
Author(s):  
ED Jatau ◽  
OB Toma ◽  
OJ Egesie ◽  
DO Damulak ◽  
Z Ayuba ◽  
...  
Keyword(s):  
Teaching Hospital ◽  
Clinical Information ◽  
Redox Status ◽  
G6pd Activity ◽  
University Teaching ◽  
Activity Levels ◽  
Evaluation Tool ◽  
Plateau State ◽  
Glucose 6 Phosphate Dehydrogenase

Red blood cell glucose-6-phosphate dehydrogenase (G6PD) is a key regulatory enzyme with the major role of meeting the cellular need for reductive biosynthesis and maintenance of redox status. G6PD deficiency is a common inherited enzyme defect associated with severe neonatal hyperbilirubinaemia that can result in permanent neurologic damage or death. This study was aimed at estimating the level of G6PD activity among icteric neonates to assess its usefulness in the evaluation of icteric neonates in Jos. One hundred and fifty icteric neonates (92 males and 58 females) whose parents consented were consecutively enrolled as they presented at the Special Care Baby Units (SCBU) of the Jos University Teaching Hospital (JUTH), Bingham University Teaching Hospital (BhUTH), and the Plateau State Specialist Hospital (PSSH), Jos. These subjects had their G6PD activity levels assayed using the Pointe Quantitative Diagnostic Kit (USA) while other relevant clinical information was obtained using a questionnaire. G6PD activity of the icteric neonates ranged between 0.54 and 24.18 IU/gHb with a mean of 8.02 ± 4.87 IU/gHb. Sixty-one (40.7 %), comprising 45 males and 16 female neonates were G6PD deficient with mean G6PD activity of 3.79 ± 1.37 IU/gHb while eighty-nine (59.3%) were G6PD normal with a mean G6PD activity of 10.92 ± 4.24 IU/gHb. G6PD activity in icteric neonates in Jos varies widely with a relatively high proportion of these neonates being G6PD deficient. Determination of G6PD activity in icteric neonates should therefore form an important evaluation tool for identification and intervention in those with the deficiency.

NAD(P)-Driven Redox Status Contributes to Desiccation Tolerance in Acer seeds

2020 ◽  
Vol 61 (6) ◽  
pp. 1158-1167 ◽  
Author(s):  
Shirin Alipour ◽  
Natalia Wojciechowska ◽  
Ewelina Stolarska ◽  
Karolina Bilska ◽  
Ewa Marzena Kalemba
Keyword(s):  
Enzyme Activity ◽  
Desiccation Tolerance ◽  
Reducing Power ◽  
High Sensitivity ◽  
Redox Status ◽  
Redox Signaling ◽  
Embryonic Axes ◽  
Antioxidant Responses ◽  
Norway Maple

Abstract Desiccation tolerance is a developmental program enabling seed survival in a dry state and is common in seeds categorized as orthodox. We focused on NAD and its phosphorylated form (NADP) because their continual switching between reduced (NAD(P)H) and oxidized (NAD(P)+) forms is involved in the modulation of redox signaling and the determination of the reducing power and further antioxidant responses. Norway maple and sycamore seeds representing the orthodox and recalcitrant categories, respectively, were used as models in a comparison of responses to water loss. The process of desiccation up to 10% water content (WC) was monitored in Norway maple seeds, while dehydration up to 30% WC was monitored in desiccation-sensitive sycamore seeds. Norway maple and sycamore seeds, particularly their embryonic axes, exhibited a distinct redox status during dehydration and desiccation. High NADPH levels, NAD+ accumulation, low and stable NAD(P)H/NAD(P)+ ratios expressed as reducing power and high NADPH-dependent enzyme activity were reported in Norway maple seeds and were considered attributes of orthodox-type seeds. The contrasting results of sycamore seeds contributed to their low antioxidant capacity and high sensitivity to desiccation. NADPH deficiency, low NADPH-dependent enzyme activity and lack of NAD+ accumulation were primary features of sycamore seeds, with implications for their NAD(P)H/NAD(P)+ ratios and reducing power and with effects on many seed traits. Thus, we propose that the distinct levels of pyridine nucleotides and their redox status contribute to orthodox and recalcitrant phenotype differentiation in seeds by affecting cellular redox signaling, metabolism and the antioxidant system.

scholarly journals Glucose-6- Phosphate Dehydrogenase Activity in Newborn in Jos: A Necessary Evaluation for Icteric Neonates

2019 ◽  
Vol 2 (4) ◽  
pp. 191-196
Author(s):  
ED Jatau ◽  
OB Toma ◽  
OJ Egesie ◽  
DO Damulak ◽  
Z Ayuba ◽  
...  
Keyword(s):  
Teaching Hospital ◽  
Clinical Information ◽  
Redox Status ◽  
G6pd Activity ◽  
University Teaching ◽  
Activity Levels ◽  
Evaluation Tool ◽  
Plateau State ◽  
Glucose 6 Phosphate Dehydrogenase

Red blood cell glucose-6-phosphate dehydrogenase (G6PD) is a key regulatory enzyme with the major role of meeting the cellular need for reductive biosynthesis and maintenance of redox status. G6PD deficiency is a common inherited enzyme defect associated with severe neonatal hyperbilirubinaemia that can result in permanent neurologic damage or death. This study was aimed at estimating the level of G6PD activity among icteric neonates to assess its usefulness in the evaluation of icteric neonates in Jos. One hundred and fifty icteric neonates (92 males and 58 females) whose parents consented were consecutively enrolled as they presented at the Special Care Baby Units (SCBU) of the Jos University Teaching Hospital (JUTH), Bingham University Teaching Hospital (BhUTH), and the Plateau State Specialist Hospital (PSSH), Jos. These subjects had their G6PD activity levels assayed using the Pointe Quantitative Diagnostic Kit (USA) while other relevant clinical information was obtained using a questionnaire. G6PD activity of the icteric neonates ranged between 0.54 and 24.18 IU/gHb with a mean of 8.02 ± 4.87 IU/gHb. Sixty-one (40.7 %), comprising 45 males and 16 female neonates were G6PD deficient with mean G6PD activity of 3.79 ± 1.37 IU/gHb while eighty-nine (59.3%) were G6PD normal with a mean G6PD activity of 10.92 ± 4.24 IU/gHb. G6PD activity in icteric neonates in Jos varies widely with a relatively high proportion of these neonates being G6PD deficient. Determination of G6PD activity in icteric neonates should therefore form an important evaluation tool for identification and intervention in those with the deficiency.

scholarly journals Novel Approach for the Determination of the Redox Status of Homocysteine and Other Aminothiols in Plasma from Healthy Subjects and Patients with Ischemic Stroke

2001 ◽  
Vol 47 (6) ◽  
pp. 1031-1039 ◽  
Author(s):  
Robert H Williams ◽  
Jack A Maggiore ◽  
Robert D Reynolds ◽  
Cathy M Helgason
Keyword(s):  
Ischemic Stroke ◽  
Whole Blood ◽  
Sodium Citrate ◽  
Redox Status ◽  
Reversed Phase ◽  
Sample Collection ◽  
Stroke Patients ◽  
Novel Approach ◽  
Plasma Thcy

Abstract Background: Plasma “redox” status can be assessed by measurements of reduced (r)-, free (f)-, oxidized (ox)-, and protein-bound (b)-homocysteine (Hcy) plus the related aminothiols cysteine, cysteinylglycine (CysGly), and glutathione (GSH), but sample collection has been complex. The redox status has not been determined in ischemic stroke patients and may provide increased understanding of its role in pathogenesis. We wished to examine the feasibility of this measurement in samples collected in readily available acidic sodium citrate. Methods: We measured aminothiols and their stability in stabilized protein-free filtrate using acidic sodium citrate (BioPool® StabilyteTM, pH 4.3) vs EDTA whole blood. Before analysis, plasma samples were also ultrafiltered to obtain a protein-free filtrate. The concentrations of total Hcy (tHcy), fHcy, and rHcy and their related aminothiols, cysteine, cysteinylglycine, and glutathione were simultaneously determined on acidic sodium-citrated blood using reversed-phase HPLC with fluorescence detection. Bound and oxidized aminothiols were calculated by difference using the concentrations of the total, free, and reduced fractions. Using this approach, we compared the redox status in newly diagnosed ischemic stroke patients (n = 20) and healthy age- and sex-matched subjects (n = 20). Results: tHcy, tCys, tCysGly, and tGSH concentrations in whole blood with Stabilyte were stable for 8 h; the reduced fraction of each aminothiol was stable for 4 h. Recovery in the protein-free filtrate was 90–100% for all reduced thiols in acidified sodium-citrated blood. Patients with ischemic stroke had higher plasma tHcy, fHcy, bHcy, rHcy, and oxHcy (P &lt;0.0005) and higher plasma t-, f-, r-, and oxCys (P &lt;0.05). t-, b-, and rCysGly concentrations were lower in the stroke patients (P &lt;0.05), as were t-, b-, and oxGSH (P &lt;0.005). Conclusions: Collection of blood in acidic sodium citrate (BioPool Stabilyte) permits the determination of the redox status of Hcy and its related aminothiols, which may add to the understanding of their relationship to the etiology of cerebrovascular disease.

Comparison of three thiol probes for determination of apoptosis-related changes in cellular redox status

2013 ◽  
Vol 85 (2) ◽  
pp. 179-187 ◽  
Author(s):  
Mette E. Skindersoe ◽  
Soeren Kjaerulff
Keyword(s):  
Redox Status ◽  
Cellular Redox
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