Screening for Anticancer Activity: DNA Fragmentation Assay

2022 ◽  
pp. 439-442
Author(s):  
InamulHasan Madar ◽  
Ghazala Sultan ◽  
Ramachandran Chelliah ◽  
Deog-Hwan Oh
Keyword(s):  
Anticancer Activity ◽  
Dna Fragmentation

LEAVES OF ANDROGRAPHIS PANICULATA IS AN ANTIOXIDANT AND ANTICANCER AGENT

2020 ◽  
pp. 213-217
Author(s):  
S. ANNAI THERASA ◽  
G. SOBIYA ◽  
S. MABEL PARIMALA
Keyword(s):  
Anticancer Activity ◽  
Dna Fragmentation ◽  
Ethanol Extract ◽  
Andrographis Paniculata ◽  
Phytochemical Analysis ◽  
Leaf Sample ◽  
Antioxidant Power ◽  
Traditional System ◽  
Promising Source

Objective: Andrographis paniculata (Family: Acanthaceae) is a well-known medicinal plant used in the Indian traditional system of medicine for the treatment of many chronic diseases. The present study was aimed to quantify secondary metabolites, determine antioxidant, and anticancer activity of ethanol extract of A. paniculata leaves. Methods: Leaf sample was macerated with ethanol solvent. Alkaloids, terpenoids, saponins, phenols, and flavonoids were quantified with standard calibrations. The antioxidant potential was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. In vitro anticancer activity was evaluated using human epithelial type 2 (HEp-2) cell line. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to estimate the cytotoxicity of the extracts. Apoptotic and necrotic effects were characterized by DNA fragmentation assay and fluorescence microscopy using the dual acridine orange/ethidium bromide (AO/EB) staining method. Results: The phytochemical analysis reveals the presence of alkaloids, saponins, phenols, flavonoids, terpenoids, and steroids. Alkaloids, terpenoids, saponins, phenol, and flavonoid content were recorded as follows: 9.84%, 8.42%, 13.94%, 44.37 mg gallic acid equivalent/100 g, and 904 mg quercetin equivalent/100 g, respectively. The antioxidant activity from DPPH, ABTS, and FRAP assays showed dose-dependent inhibition of free radicals. In cell viability tests, cell death with increasing extract concentration was observed. DNA fragmentation and AO/EB stain confirmed apoptosis and necrosis in extract-treated cells. Conclusion: The results indicate that A. paniculata is a promising source for the development of antioxidant and anticancer drugs.

scholarly journals Apoptosis Induced by (−)-Epicatechin in Human Breast Cancer Cells is Mediated by Reactive Oxygen Species

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1020 ◽  
Author(s):  
Fernando Pereyra-Vergara ◽  
Ivonne María Olivares-Corichi ◽  
Adriana Guadalupe Perez-Ruiz ◽  
Juan Pedro Luna-Arias ◽  
José Rubén García-Sánchez
Keyword(s):  
Breast Cancer ◽  
Reactive Oxygen Species ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Dna Fragmentation ◽  
Mechanism Of Action ◽  
Breast Cancer Cells ◽  
Death Receptor ◽  
Oxygen Species ◽  
Mcf 7

(−)-Epicatechin is a phenolic compound with antioxidant activity that is present in natural food and drinks, such as cocoa and red wine. Evidence suggests that (−)-epicatechin exhibits anticancer activity; however, its mechanism of action is poorly understood. Here, we investigated the anticancer effects of (−)-epicatechin and its mechanism of action in breast cancer cells. We assessed the anticancer activity by cell proliferation assays, apoptosis by DNA fragmentation and flow cytometry. The expression of proteins associated with apoptosis was analyzed by the human apoptosis array. MitoSOXTM Red and biomarkers of oxidative damage were used to measure the effect of (−)-epicatechin on mitochondrial reactive oxygen species (ROS) and cellular damage, respectively. (−)-Epicatechin treatment caused a decreasing in the viability of MDA-MB-231 and MCF-7 cells. This cell death was associated with DNA fragmentation and an apoptotic proteomic profile. Further, (−)-epicatechin in MDA-MB-231 cells upregulated death receptor (DR4/DR5), increased the ROS production, and modulated pro-apoptotic proteins. In MCF-7 cells, (−)-epicatechin did not involve death receptor; however, an increase in ROS and the upregulation of pro-apoptotic proteins (Bad and Bax) were observed. These changes were associated with the apoptosis activation through the intrinsic pathway. In conclusion, this study shows that (−)-epicatechin has anticancer activity in breast cancer cells and provides novel insight into the molecular mechanism of (−)-epicatechin to induce apoptosis.

DNA-fragmentation and expression of apoptosis-related proteins in experimentally denervated and reinnervated rat facial muscle

1997 ◽  
Vol 23 (3) ◽  
pp. 141-149 ◽  
Author(s):  
D. S. Tews ◽  
H. H. Goebel ◽  
I. Schneider ◽  
A. Gunkel ◽  
E. Stennert ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Facial Muscle ◽  
Related Proteins

883: The Anticancer Activity of Mycobacterial Cell Wall-DNA Complex (MCC) Against Human Bladder Cancer Cell Lines is Different to BCG

2007 ◽  
Vol 177 (4S) ◽  
pp. 294-294
Author(s):  
Nigel C. Phillips ◽  
Sonia Ménard ◽  
David S. Surprenant ◽  
Danbing Ke ◽  
Mario C. Filion
Keyword(s):  
Bladder Cancer ◽  
Cell Wall ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Human Bladder Cancer Cell ◽  
Mycobacterial Cell Wall ◽  
Dna Complex

Novel LC-MS/MS approaches for metabolite identification of natural compounds, such as curcumin and analogues, with anticancer activity

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
C Tamvakopoulos ◽  
ZD Sofianos ◽  
AL Skaltsounis ◽  
M Kritsanida
Keyword(s):  
Anticancer Activity ◽  
Metabolite Identification ◽  
Natural Compounds

Anticancer activity of resveratrol analogs from Eugenia rigida DC

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
MA Zaki ◽  
V Samoylenko ◽  
S Khan ◽  
RM Abd slam ◽  
MH Hetta ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Resveratrol Analogs
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