LEAVES OF ANDROGRAPHIS PANICULATA IS AN ANTIOXIDANT AND ANTICANCER AGENT

2020 ◽  
pp. 213-217
Author(s):  
S. ANNAI THERASA ◽  
G. SOBIYA ◽  
S. MABEL PARIMALA
Keyword(s):  
Anticancer Activity ◽  
Dna Fragmentation ◽  
Ethanol Extract ◽  
Andrographis Paniculata ◽  
Phytochemical Analysis ◽  
Leaf Sample ◽  
Antioxidant Power ◽  
Traditional System ◽  
Promising Source

Objective: Andrographis paniculata (Family: Acanthaceae) is a well-known medicinal plant used in the Indian traditional system of medicine for the treatment of many chronic diseases. The present study was aimed to quantify secondary metabolites, determine antioxidant, and anticancer activity of ethanol extract of A. paniculata leaves. Methods: Leaf sample was macerated with ethanol solvent. Alkaloids, terpenoids, saponins, phenols, and flavonoids were quantified with standard calibrations. The antioxidant potential was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. In vitro anticancer activity was evaluated using human epithelial type 2 (HEp-2) cell line. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to estimate the cytotoxicity of the extracts. Apoptotic and necrotic effects were characterized by DNA fragmentation assay and fluorescence microscopy using the dual acridine orange/ethidium bromide (AO/EB) staining method. Results: The phytochemical analysis reveals the presence of alkaloids, saponins, phenols, flavonoids, terpenoids, and steroids. Alkaloids, terpenoids, saponins, phenol, and flavonoid content were recorded as follows: 9.84%, 8.42%, 13.94%, 44.37 mg gallic acid equivalent/100 g, and 904 mg quercetin equivalent/100 g, respectively. The antioxidant activity from DPPH, ABTS, and FRAP assays showed dose-dependent inhibition of free radicals. In cell viability tests, cell death with increasing extract concentration was observed. DNA fragmentation and AO/EB stain confirmed apoptosis and necrosis in extract-treated cells. Conclusion: The results indicate that A. paniculata is a promising source for the development of antioxidant and anticancer drugs.

scholarly journals A Study on Phytochemical and Anticancer Activities of Epiphytic Orchid Aerides odorata Lindl.

2019 ◽  
pp. 1-21 ◽  
Author(s):  
Jhansi Katta ◽  
Venkatesh Rampilla ◽  
S. M. Khasim
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Cancer Cell ◽  
Eastern Ghats ◽  
Phytochemical Analysis ◽  
Anticancer Activities ◽  
Cancer Cell Growth ◽  
Ms Analysis ◽  
Promising Source

Aim: The present study was carried out to evaluate the phytochemical composition and anticancer activities of leaf extract of Aerides odorata Lour., a widely distributed epiphytic herb found in the Eastern Ghats of Vizianagram district. Methodology: The solvents like n-hexane, ethyl acetate and methanol were used to extract dried leaf material of A. odorata. These extracts were analysed for phytochemical constituents by GC-MS analysis and in vitro anticancer activity was done against two cancer cell lines (MCF-7 and HeLa cell line) by using MTT assay. Results: Preliminary phytochemical analysis revealed the presence of alkaloids, coumarins, flavonoids, glycosides, phenols, and terpenoids. GC-MS analysis determines presence of 15 compounds in ethyl acetate and 14 compounds in methanol extracts respectively. Among                    two extracts a total 13 compounds have anticancer activity. Both the solvent extracts exhibit significant cancer cell growth inhibition with IC50 value ranging between 26.211 µg/mL to 59.061 µg/mL. Conclusion: Methanol about the best solvent and its activity. Our result showed A. odorata is a promising source of anticancer drugs.

scholarly journals In vitro antioxidant and anticancer activity of crude ethanol extract of Mahkota Dewa (Phaleria macrocarpa) leaves

2021 ◽  
Author(s):  
Yuyun Ika Christina ◽  
Wirdatun Nafisah ◽  
Widodo ◽  
Muhaimin Rifa’i ◽  
Muhammad Sasmito Djati
Keyword(s):  
Anticancer Activity ◽  
Ethanol Extract ◽  
Phaleria Macrocarpa

Phytochemical, In vitro Anti-inflammatory and Antimicrobial Potential of Hugonia mystax L.

2021 ◽  
pp. 169-173
Author(s):  
K.P. Jaiganesh ◽  
T.J. Jasna ◽  
A.C. Tangavelou
Keyword(s):  
Tamil Nadu ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Membrane Stabilization ◽  
Traditional System ◽  
Antimicrobial Potential ◽  
Biochemical Compounds ◽  
Anti Inflammatory

Hugonia mystax L., (Linaceae), is commonly distributed in the thorny scrubs and tropical dry evergreen forests of Tamil Nadu, which has been valued for centuries in traditional system of medicine for the treatment of various ailments. In the present study was an attempt to investigate the phytochemical nature and anti-inflammatory, antimicrobial potential by adopting suitable methods. Phytochemical analysis of Hugonia mystax L., plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, triterpenoids and saponins etc. Since triterpenoids and flavonoids have remarkable anti-inflammatory activity, so our present work aims at evaluating in vitro anti inflammatory activity of Hugonia mystax L., by HRBC membrane stabilization method. The inhibition of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization for ethanolic extracts and Diclofenac sodium were done at different concentrations. The maximum membrane stabilization of Hugonia mystax L., extracts was found to be 94.97 % at a dose of 2000 μg/ml. Therefore, our studies support the isolation and the use of active constituents from Hugonia mystax L., in treating inflammations.

scholarly journals Evaluation of In Vitro Antioxidant and Antidiabetic Activities of Aristolochia longa Extracts

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Nasreddine El Omari ◽  
Karima Sayah ◽  
Saad Fettach ◽  
Omar El Blidi ◽  
Abdelhakim Bouyahya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Antioxidants ◽  
Ethyl Acetate Fraction ◽  
Inhibitory Effect ◽  
Phytochemical Analysis ◽  
Aqueous Fraction ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Therapeutic Tools

Oxidative stress plays a major role in diabetic physiopathology; hence, the interest of using natural antioxidants as therapeutic tools exists. The aim of this study was the evaluation of in vitro antioxidant activity and inhibitory potential of organic extracts from Aristolochia longa roots against key enzymes linked to hyperglycemia. Antioxidant activity was performed using 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals and ferric reducing/antioxidant power (FRAP) methods. The α-Glucosidase and β-Galactosidase inhibitory activities were investigated using an in vitro model. Moreover, phytochemical analysis of tested extracts was carried out. The aqueous fraction of this herb exhibited the highest antioxidant activity for both DPPH and ABTS methods, IC50=125.40±2.40 μg/mL and IC50=65.23±2.49 μg/mL, respectively. However, the ethyl acetate fraction possessed the strongest inhibitory effect towards α-Glucosidase (IC50=1.112±0.026 mg/mL). Furthermore, the result showed high levels of phenolic content. The results showed that this plant could be a significant source of medically important natural compounds.

scholarly journals IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITY OF ULVA LACTUCA L.USING MOLT-3 CELL LINE

2019 ◽  
pp. 75-78 ◽  
Author(s):  
CHIDAMBARARAJAN P ◽  
KEERTHANA V ◽  
PRIYADHARSHINI K, ◽  
SAKTHIVEL B
Keyword(s):  
Anticancer Activity ◽  
Cell Line ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Ethanol Extract ◽  
Maximum Growth ◽  
Ulva Lactuca ◽  
Cell Proliferation Inhibition ◽  
Free Radical Scavenging Assay

Objective: The aim of the present investigation was to determine the in vitro antioxidant and anticancer activity of the ethanol extract of Ulva lactuca L. Methods: The present study was to investigate the antioxidant and anticancer activity of U. lactuca L. The extract of U. lactuca L. was extracted by ethanol and subject to analysis. An in vitro antioxidant activity of the ethanol extract of U. lactuca L. was performed by 1, 1-diphenyl-2-picrylhydrazyl free radical scavenging assay. Simultaneously anticancer activity was also performed using blood cancer (MOLT-3) cell line, and the species showed a strong selective cell proliferation inhibition of the cancer cell line. Results: The scavenging activity was measured and determined to be 78.5%. This might be due to high polyphenolic compounds and flavonoid contents of the extract, which showed maximum growth inhibition of 74.4%. Conclusion: Thus, the study concludes that the constituents of seaweeds can act as potent in treating various diseases and can be used as an alternative for therapeutic treatment.

scholarly journals In vitro antiplasmodial, cytotoxic and antioxidant effects, and phytochemical constituents of eleven plants used in the traditional treatment of malaria in Akwa Ibom State, Nigeria

2021 ◽  
Vol 20 (1) ◽  
pp. 105-111
Author(s):  
Olorunfemi Abraham Eseyin ◽  
Iweh Emmanuel Etim ◽  
Emmanuel E. Attih ◽  
Ekarika Johnson ◽  
Aniefiok Sunday Udobre ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Antiplasmodial Activity ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Cytotoxicity Test ◽  
Chromolaena Odorata ◽  
Ocimum Gratissimum ◽  
Saponin Content ◽  
Milicia Excelsa

Purpose: To evaluate the antiplasmodial effects of eleven plants (Bombax buonopozense, Carica papaya, Anthocleista djalonensis, Milicia excelsa, Heterotis rotundifolia, Homalim letestui, Starchystarpheta cayennnensis, Ocimum gratissimum, Cleistopholis patens, Chromolaena odorata and Hippocratea africana) reportedly used in the treatment of malaria in Akwa Ibom State of Nigeria. Methods: Phytochemical analysis was done by standard methods, while in vitro antiplasmodial evaluation was carried out using Plasmodium falciparum chloroquine-sensitive and chloroquineresistant strains using lactate dehydrogenase (pLDH) assay.  Cytotoxicity test was undertaken by MTT assay on LLC-MK2 cells and the concentration killing 50 % of the cells (CC50) was calculated.Antioxidant activity of the ethanol extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Results: Milicia excelsa, Heterotis rotundifolia and Chromolaena odorata had moderate antiplasmodial activity. Ocimum gratissimum and Hippocratea africana were weakly active. Milicia excelsa showed a considerable level of cytotoxicity, while Bombax buonopozense exhibited moderate cytotoxicity. Bombax buonopozense (95.3 %) and Ocimum gratissimum (92.0 %) exhibited high DPPH scavengingeffect comparable to Vitamin C (98.7 %). There was a significant correlation (p < 0.05) between DPPH inhibition and the total phenolic contents of the eleven plants studied (r2 = 0.6616), between DPPH inhibition and flavonoids (r2 = 0.3553), between antiplasmodial activity and saponin content (r2= 0.3992), and between the two antiplasmodial evaluation assay methods (r2 = 0.614).Conclusion: The results of this work provide some justification for the use of Milicia excelsa, Heterotis rotundifolia, Chromolaena odorata, Ocimum gratissimum and Hippocratea africana in the treatment of malaria. Keywords: Antiplasmodial, Antioxidant, Cytotoxicity, Phytochemicals, Antimalaria

scholarly journals Comparative extraction, phytochemical screening and in vitro biological activities of Eclipta prostrata extract

2020 ◽  
Vol 10 (4-s) ◽  
pp. 148-152
Author(s):  
Zafar Alam Khan ◽  
Salaj Khare ◽  
B.K. Dubey
Keyword(s):  
Therapeutic Potential ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Antidiabetic Activity ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Eclipta Prostrata ◽  
Promising Source ◽  
Dose Dependent

Medicinal plants possess therapeutic potential and are used to treat various diseases around the world. Eclipta prostrate (L.) is a medicinal herb that has extensive application in the native medicinal system. In any therapeutic activity chemical constituents play an important role. Eclipta prostrata has been investigated in this study for its antioxidant, antimicrobial and antidiabetic activity in vitro. The well-known research protocol available in the literature established qualitative analysis of the different phytochemical constituents and quantitative analysis of total phenol and flavonoids. The hydroalcoholic extracts of the leaves and seeds of Eclipta prostrata exhibited significant and dose-dependent antioxidant activity including ability to donate electron. To analyze the antimicrobial activity, Leaves hydroalcoholic extracts and Eclipta prostrate seeds were tested against two selected strains using a well-diffusion method and showing significant inhibitory action against all the strain tested. In addition, the dose-dependent α-amylase inhibitory activity with an IC50 value of acarbose, leaves, and seed extract was found to be 364.89μg/ml and 438.43μg/ml, respectively, indicating that Eclipta prostrate is a promising source as an herbal medicine. Keywords: Eclipta prostrate, Phytochemical Analysis, Antioxidant, Antimicrobial, Antidiabetic Activity.

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