pH/Acetonitrile-Gradient Reversed-Phase Fractionation of Enriched Hyper-Citrullinated Library in Combination with LC–MS/MS Analysis for Confident Identification of Citrullinated Peptides

2021 ◽  
pp. 107-126
Author(s):  
Aneta Stachowicz ◽  
Niveda Sundararaman ◽  
Vidya Venkatraman ◽  
Jennifer Van Eyk ◽  
Justyna Fert-Bober
Keyword(s):  
Reversed Phase ◽  
Ms Analysis ◽  
Acetonitrile Gradient

Amino Acid Composition of Cockroach Hypertrehalosaemic Hormones

1985 ◽  
Vol 40 (1-2) ◽  
pp. 42-46 ◽  
Author(s):  
Gerd Gäde
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
High Performance ◽  
Periplaneta Americana ◽  
Methanesulfonic Acid ◽  
Reversed Phase ◽  
American Cockroach ◽  
Amino Acid Residues ◽  
Corpus Cardiacum ◽  
Acetonitrile Gradient

Hypertrehalosaemic hormones I and II from the corpus cardiacum of the American cockroach (Periplaneta americana) were separated by reversed-phase high-performance liquid chromato­graphy using a Nucleosil C18 column with a trifluoroacetic acid/acetonitrile gradient. The eluent was monitored at 206 nm and the hypertrehalosaemic activity detected by bioassay. The amino acid compositions of hypertrehalosaemic hormone I and II were determined after acid hydrolysis with HCl or methanesulfonic acid. Both neurohormones are octapeptides. Hypertrehalosaemic hormone I contained the amino acids Asp(2), Ser, Glu, Pro, Val, Phe and Trp, whereas hyper­trehalosaemic hormone II contained the amino acid residues Asp, Thr(2), Glu, Pro, Leu, Phe and Trp.

scholarly journals Reversed-phase HPLC/MS analysis of fatty acid 9-anthrylmethyl ester derivatives

2004 ◽  
Vol 53 (6) ◽  
pp. 533-539 ◽  
Author(s):  
Kazuhiko NISHIMURA ◽  
Toshiyuki SUZUKI ◽  
Eiji KATSURA ◽  
Yutaka ITABASHI
Keyword(s):  
Fatty Acid ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Ms Analysis

High Perform ance Liquid Chrom atographie Analysis o f Steroidal Saponins from Avena sativa L

1981 ◽  
Vol 36 (11-12) ◽  
pp. 1072-1074 ◽  
Author(s):  
J. Kesselmeier ◽  
D. Strack
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Avena Sativa ◽  
High Performance ◽  
Reversed Phase ◽  
Detector Response ◽  
Gradient System ◽  
Steroidal Saponins ◽  
Uv Detector ◽  
Acetonitrile Gradient

Abstract Reversed phase high performance liquid chromatography offers an efficient and rapid method for analysis of steroidal saponins. Crude extracts from primary leaves of Avena sativa and isolated etioplasts therefrom have been resolved into four saponins (avenacosides) using a water-acetonitrile gradient system on RP-8 and monitoring the column effluent at 200 nm with an UV-detector. Detectability was found to be in the range of 50 ng avenacoside B and the detector response was linear up to 8 μg tested. The described method is applicable to studies on localization and physiology of Avena saponins during development of the primary leaf.

scholarly journals Proteomics Analyses of the Opportunistic PathogenBurkholderia vietnamiensisUsing Protein Fractionations and Mass Spectrometry

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Samanthi Wickramasekara ◽  
Julie Neilson ◽  
Naren Patel ◽  
Linda Breci ◽  
Amy Hilderbrand ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Virulence Factors ◽  
Protein Identification ◽  
Reversed Phase ◽  
Tandem Mass ◽  
Burkholderia Vietnamiensis ◽  
Ms Analysis ◽  
Liquid Chromatography Tandem Mass ◽  
Identification Technique

The main objectives of this work were to obtain a more extensive coverage of theBurkholderia vietnamiensisproteome than previously reported and to identify virulence factors using tandem mass spectrometry. The proteome ofB. vietnamiensiswas precipitated into four fractions to as extracellular, intracellular, cell surface and cell wall proteins. Two different approaches were used to analyze the proteins. The first was a gel-based method where 1D SDS-PAGE was used for separation of the proteins prior to reverse phase liquid chromatography tandem mass spectrometry (LC-MS/MS). The second method used MudPIT analysis (Multi dimensional Protein Identification Technique), where proteins are digested and separated using cation exchange and reversed phase separations before the MS/MS analysis (LC/LC-MS/MS). Overall, gel-based LC-MS/MS analysis resulted in more protein identifications than the MudPIT analysis. Combination of the results lead to identification of more than 1200 proteins, approximately 16% of the proteins coded from the annotated genome ofBurkholderiaspecies. Several virulence factors were detected including flagellin, porin, peroxiredoxin and zinc proteases.

Reversed-phase HPLC-ESI/MS analysis of birch leaf proanthocyanidins after their acidic degradation in the presence of nucleophiles

2007 ◽  
Vol 18 (5) ◽  
pp. 378-386 ◽  
Author(s):  
Maarit Karonen ◽  
Anne Leikas ◽  
Jyrki Loponen ◽  
Jari Sinkkonen ◽  
Vladimir Ossipov ◽  
...  
Keyword(s):  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Esi Ms ◽  
Ms Analysis ◽  
Acidic Degradation
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