Stress Protein Gene Expression in Amphibians

1997 ◽  
pp. 137-164
Author(s):  
John J. Heikkila ◽  
Adnan Ali ◽  
Nick Ohan ◽  
Ying Tam
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Stress Protein

Influence of bioenergetic stress on heat shock protein gene expression in nucleated red blood cells of fish

1999 ◽  
Vol 276 (4) ◽  
pp. R990-R996 ◽  
Author(s):  
Suzanne Currie ◽  
Bruce L. Tufts ◽  
Christopher D. Moyes
Keyword(s):  
Gene Expression ◽  
Red Blood Cells ◽  
Gene Transcription ◽  
Blood Cells ◽  
Protein Gene ◽  
Stress Protein ◽  
Lactate Production ◽  
Induce Stress ◽  
Energy Status ◽  
Nucleated Red Blood Cells

The physiological and biochemical signals that induce stress protein (HSP) synthesis remain conjectural. In this study, we used the nucleated red blood cells from rainbow trout, Oncorhynchus mykiss, to address the interaction between energy status and HSP gene expression. Heat shock (25°C) did not significantly affect ATP levels but resulted in an increase in HSP70 mRNA. Hypoxia alone did not induce HSPtranscription in these cells despite a significant depression in ATP. Inhibition of oxidative phosphorylation with azide, in the absence of thermal stress, decreased ATP by 56% and increased lactate production by 62% but did not induce HSP gene transcription. Inhibition of oxidative phosphorylation and glycolysis with azide and iodoacetic acid respectively, decreased ATP by 79% and prevented lactate production, but did not induce either HSP70 or HSP30 gene transcription in these cells. This study demonstrates that a reduction in the cellular energy status will not induce stress protein gene transcription in rainbow trout red blood cells and may, in fact, limit induction during extreme metabolic inhibition.

Enhanced accumulation of mRNA for 78-kilodalton glucose-regulated protein (GRP78) in tissues of nonobese diabetic mice

1990 ◽  
Vol 68 (12) ◽  
pp. 1428-1432 ◽  
Author(s):  
Craig L. J. Parfett ◽  
Katarzyna Brudzynski ◽  
Calvin Stiller
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Stress Protein ◽  
Diabetic Mice ◽  
Specific Expression ◽  
Nonobese Diabetic ◽  
Elevated Expression ◽  
Relevant Regulation ◽  
Nonobese Diabetic Mice ◽  
Glucose Regulated Protein

Using 78-kilodalton glucose-regulated protein cDNA as a probe of Northern blots, we have examined the distribution and inducibility of mRNA encoding the 78-kilodalton glucose-regulated protein in three tissues of nonobese diabetic mice. The gene was constitutively expressed in normal, unstressed cells of liver, brain, and spleen. Developing diabetes correlated with elevated expression in only liver and brain of diabetic mice. This induction of gene expression was associated with the transition from the prediabetic stage to the onset of hyperglycemia and coincided with falling levels of plasma insulin and rising hyperglycemia. The activation of 78-kilodalton glucose-regulated protein gene expression appeared to be transient. We suggest that the temporally differential, tissue-specific expression of this gene in adult nonobese diabetic mice offers an opportunity to study a physiologically relevant regulation of this stress-induced gene.Key words: stress protein, gene expression, diabetes, liver, brain.

Induction of heat-shock (stress) protein gene expression by selected natural and anthropogenic disturbances in the octocoral Dendronephthya klunzingeri

2000 ◽  
Vol 245 (2) ◽  
pp. 265-276 ◽  
Author(s):  
Matthias Wiens ◽  
Mohammed S.A Ammar ◽  
Ahmed H Nawar ◽  
Claudia Koziol ◽  
Hamdy M.A Hassanein ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Protein Gene ◽  
Stress Protein ◽  
Anthropogenic Disturbances ◽  
Heat Shock Stress ◽  
Shock Stress

Early Stress Protein Gene Expression in a Human Model of Ischemic Preconditioning

2004 ◽  
Vol 78 (10) ◽  
pp. 1479-1487 ◽  
Author(s):  
Anisha Patel ◽  
Marcel C. G. van de Poll ◽  
Jan W. M. Greve ◽  
Wim A. Buurman ◽  
Kenneth C. H. Fearon ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ischemic Preconditioning ◽  
Protein Gene ◽  
Stress Protein ◽  
Human Model ◽  
Early Stress

Stress protein gene expression in ischemic-reperfused livers

1990 ◽  
Vol 14 ◽  
pp. 185
Author(s):  
L SCHIAFFONATI ◽  
L TACCHINI ◽  
E RAPPOCCIOLO ◽  
G CAIRO ◽  
A BERNELLIZAZZERA
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Stress Protein

Interplay between Pur α and Egr-1 in the transcriptional regulation of amyloid precursor protein gene expression

2010 ◽  
Vol 34 (8) ◽  
pp. S27-S27
Author(s):  
Jianqi Cui ◽  
Xiuying Pei ◽  
Qian Zhang ◽  
Bassel E. Sawaya ◽  
Xiaohong Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Amyloid Precursor Protein ◽  
Protein Gene ◽  
Precursor Protein ◽  
Amyloid Precursor Protein Gene

scholarly journals Serine-rich ultra high sulfur protein gene expression in murine hair and skin during the hair cycle.

1990 ◽  
Vol 265 (34) ◽  
pp. 21375-21380
Author(s):  
L Wood ◽  
M Mills ◽  
N Hatzenbuhler ◽  
G Vogeli
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Hair Cycle ◽  
Sulfur Protein

scholarly journals CdSe/ZnS Core-Shell-Type Quantum Dot Nanoparticles Disrupt the Cellular Homeostasis in Cellular Blood–Brain Barrier Models

2021 ◽  
Vol 22 (3) ◽  
pp. 1068
Author(s):  
Katarzyna Dominika Kania ◽  
Waldemar Wagner ◽  
Łukasz Pułaski
Keyword(s):  
Gene Expression ◽  
Quantum Dot ◽  
Blood Brain Barrier ◽  
Protein Gene ◽  
Brain Barrier ◽  
Core Shell ◽  
Cellular Homeostasis ◽  
Blood Brain ◽  
Shell Type ◽  
The Impact

Two immortalized brain microvascular endothelial cell lines (hCMEC/D3 and RBE4, of human and rat origin, respectively) were applied as an in vitro model of cellular elements of the blood–brain barrier in a nanotoxicological study. We evaluated the impact of CdSe/ZnS core-shell-type quantum dot nanoparticles on cellular homeostasis, using gold nanoparticles as a largely bioorthogonal control. While the investigated nanoparticles had surprisingly negligible acute cytotoxicity in the evaluated models, a multi-faceted study of barrier-related phenotypes and cell condition revealed a complex pattern of homeostasis disruption. Interestingly, some features of the paracellular barrier phenotype (transendothelial electrical resistance, tight junction protein gene expression) were improved by exposure to nanoparticles in a potential hormetic mechanism. However, mitochondrial potential and antioxidant defences largely collapsed under these conditions, paralleled by a strong pro-apoptotic shift in a significant proportion of cells (evidenced by apoptotic protein gene expression, chromosomal DNA fragmentation, and membrane phosphatidylserine exposure). Taken together, our results suggest a reactive oxygen species-mediated cellular mechanism of blood–brain barrier damage by quantum dots, which may be toxicologically significant in the face of increasing human exposure to this type of nanoparticles, both intended (in medical applications) and more often unintended (from consumer goods-derived environmental pollution).

scholarly journals Neuropeptide and calcium-binding protein gene expression profiles predict neuronal anatomical type in the juvenile rat

2005 ◽  
Vol 567 (2) ◽  
pp. 401-413 ◽  
Author(s):  
Maria Toledo-Rodriguez ◽  
Philip Goodman ◽  
Milena Illic ◽  
Caizhi Wu ◽  
Henry Markram
Keyword(s):  
Gene Expression ◽  
Calcium Binding ◽  
Protein Gene ◽  
Binding Protein ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Calcium Binding Protein ◽  
Binding Protein Gene
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