Association between Calcium Phosphate and Calcium Oxalate Crystals in the Development of Urinary Stones

1995 ◽  
pp. 261-270 ◽  
Author(s):  
S. R. Khan
Keyword(s):  
Calcium Phosphate ◽  
Calcium Oxalate ◽  
Urinary Stones ◽  
Calcium Oxalate Crystals ◽  
Oxalate Crystals

scholarly journals STUDY OF IN VITRO ANTI-LITHIATIC EFFECT OF IPOMOEA BATATAS (L) LEAVES AND TUBEROUS ROOTS

2018 ◽  
Vol 11 (2) ◽  
pp. 427 ◽  
Author(s):  
Sathish R ◽  
Jeyabalan G
Keyword(s):  
Calcium Phosphate ◽  
Calcium Oxalate ◽  
Ipomoea Batatas ◽  
Colorimetric Method ◽  
Dependent Manner ◽  
Calcium Oxalate Crystals ◽  
Tuberous Roots ◽  
Oxalate Crystals ◽  
Leaves And Roots

 Objective: The present study was to demonstrate the in vitro anti-lithiatic effect of Ipomoea batatas (Convolvulaceae) leaves and tuberous roots.Methods: The obtained ethanolic extract of I. batatas leaves and tuberous roots (EIBL and EIBR) and aqueous extract of I. batatas leaves and roots (AIBL and AIBR) were used for this in vitro study. The dissolution method of calcium oxalate by titrimetry method and calcium phosphate by colorimetric method was studied. Nucleation and aggregation of calcium oxalate crystals were determined by a spectrophotometric assay.Results: In the estimation of calcium oxalate by titrimetry method, the I. batatas leaves and roots have very significant (p<0.01) capability to dissolve calcium oxalate. Percentage dissolution of calcium oxalate crystals was found to be 37.53%, 22.74%, 39.74%, and 24.28% for EIBL, AIBL, EIBR, and AIBR, respectively. In the estimation of calcium phosphate by colorimetric method, the percentage dissolution of calcium phosphate crystals by EIBL, AIBL, EIBR, and AIBR was found to be 67.15%, 43.17%, 76.74%, and 47.96%, respectively. The I. batatas leaves and roots were significantly (p<0.01) dissolved calcium phosphate also. The results were clearly shown that I. batatas extracts significantly (p<0.01) inhibited both nucleation and aggregation of calcium oxalate crystals by concentration-dependent manner. The maximum percent inhibition of calcium oxalate nucleation by EIBL, AIBL, EIBR, and AIBR was found to be 59.09%, 50.0%, 84.09%, and 47.73%, respectively, at 1000 μg/ml. The EIBL, AIBL, EIBR, and AIBR were inhibited calcium oxalate aggregation by 63.46%, 36.54%, 84.61%, and 42.3%, respectively, at 1000 μg/ml.Conclusions: The results clearly indicate that I. batatas leaves and tuberous roots were found to express in vitro anti-lithiatic potential.

scholarly journals In-Vitro antiurolithiatic activity of aqueous extract Pavonia lasiopetala

2019 ◽  
Vol 9 (2) ◽  
pp. 102-104
Author(s):  
R. Ramprasad ◽  
Anil Nilsha ◽  
S. Shahul Hameed ◽  
Junuha M R Shifama ◽  
N. Venkateshan
Keyword(s):  
Calcium Phosphate ◽  
Calcium Oxalate ◽  
Aqueous Extract ◽  
Inhibitory Action ◽  
In Vivo Studies ◽  
Precipitation Method ◽  
Calcium Oxalate Crystals ◽  
Permeable Membrane ◽  
Oxalate Crystals

Background: Upto date, the scientific documentation regarding in-vitro antiurolithiatic activity of has been reported, although it has not reported for in-vitro antiurolithiatic activity up till now. Objective: To explore anti-urolithiatic activities of Pavonia lasiopetala leaves extract by utilizing different in-vitro models. Material and Methods: Aqueous extract of Pavonia lasiopetala fresh leaves was prepared and arranged in the different concentrations. Homogenous precipitation method was used to prepare artificial stones such as calcium oxalate and calcium phosphate and semi-permeable membrane of eggs was used as dissolution bags. Dissolution models were incubated in 72 hrs and after that, the entire content in dissolution bags was estimated spectrophotometrically. The inhibitory activity of Pavonia lasiopetala leaves extract on the nucleation of calcium oxalate crystals and the rate of aggregation in calcium oxalate crystals was determined by spectrophotometric assay. Results: In dissolution models, the extract of Pavonia lasiopetala has greater capability to dissolve calcium oxalate while Cystone standard has shown better demineralization for calcium phosphate rather than extract of Pavonia lasiopetala. Cystone exhibited strongly inhibitory action in the nucleation assay rather than aggregation assay. The extract of Pavonia lasiopetala exhibited inhibitory action in both of nucleation and aggregation assays to significant level.  Discussion: Correlation between in-vitro and in-vivo studies may be helpful to understand the molecular mechanism of litholysis process and to reveal phytochemicals of the extract responsible for dissolving or disintegrating renal calculi. Conclusion: Pavonia lasiopetala extract exhibited significant in-vitro anti-urolithiatic activity. Keywords: Pavonia lasiopetala, spectrophotometrically, in-vitro antiurolithiatic.

An SEM study of raphide crystal initials in the leaves of vitis (grape)

1995 ◽  
Vol 53 ◽  
pp. 984-985
Author(s):  
H. J. Arnott ◽  
M. A. Webb ◽  
L. E. Lopez
Keyword(s):  
Calcium Oxalate ◽  
Water Soluble ◽  
Calcium Oxalate Crystals ◽  
Calcium Oxalate Crystal ◽  
Oxalate Crystals ◽  
Large Numbers ◽  
Sem Study ◽  
The Matrix ◽  
Crystal Cells ◽  
Crystal Idioblast

Many papers have been published on the structure of calcium oxalate crystals in plants, however, few deal with the early development of crystals. Large numbers of idioblastic calcium oxalate crystal cells are found in the leaves of Vitis mustangensis, V. labrusca and V. vulpina. A crystal idioblast, or raphide cell, will produce 150-300 needle-like calcium oxalate crystals within a central vacuole. Each raphide crystal is autonomous, having been produced in a separate membrane-defined crystal chamber; the idioblast''s crystal complement is collectively embedded in a water soluble glycoprotein matrix which fills the vacuole. The crystals are twins, each having a pointed and a bidentate end (Fig 1); when mature they are about 0.5-1.2 μn in diameter and 30-70 μm in length. Crystal bundles, i.e., crystals and their matrix, can be isolated from leaves using 100% ETOH. If the bundles are treated with H2O the matrix surrounding the crystals rapidly disperses.

Calcium oxalate crystals in Dioscorea (Dioscoreaceae)

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
V Raman ◽  
IA Khan
Keyword(s):  
Calcium Oxalate ◽  
Calcium Oxalate Crystals ◽  
Oxalate Crystals

Oxalic acid and calcium oxalate produced by Leucostoma cincta and L. persoonii in culture and in peach bark tissues

1987 ◽  
Vol 65 (9) ◽  
pp. 1952-1956 ◽  
Author(s):  
J. A. Traquair
Keyword(s):  
Oxalic Acid ◽  
Calcium Oxalate ◽  
Crystal Morphology ◽  
Culture Media ◽  
Positive Staining ◽  
Acid Solutions ◽  
Calcium Oxalate Crystals ◽  
Oxalate Crystals ◽  
Electron Microscopes ◽  
Scanning Electron Microscopes

Oxalic acid and crystals of calcium oxalate were produced during growth of Leucostoma cincta and L. persoonii on potato dextrose agar and in peach bark tissues. The identification of calcium oxalate was based on solubility characteristics, the results of KMnO4 titration, positive staining with silver nitrate – dithiooxamide, and crystal morphology as observed with light and scanning electron microscopes. Oxalic acid was detected by gas chromatography. This is the first report of oxalic acid production by both Leucostoma species causing peach canker. Calcium oxalate crystals observed on or near hyphae in culture were similar to crystals in artificially inoculated peach bark tissues. Addition of oxalic acid solutions alone to inner bark tissues caused maceration and necrosis. These results indicate a role for oxalic acid in the early stages of pathogenesis by Leucostoma spp. Tetragonal (bipyramidal) and prismatic calcium oxalate crystals formed on bark wounds treated with oxalic acid solutions were similar to those observed in infected tissues and in culture media amended with oxalic acid.

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