Single-Step Antigen Loading and Maturation of Dendritic Cells Through mRNA Electroporation of a Tumor-Associated Antigen and a TriMix of Costimulatory Molecules

2014 ◽  
pp. 3-15 ◽  
Author(s):  
Daphné Benteyn ◽  
An M. T. Van Nuffel ◽  
Sofie Wilgenhof ◽  
Aude Bonehill
Keyword(s):  
Dendritic Cells ◽  
Costimulatory Molecules ◽  
Single Step ◽  
Tumor Associated Antigen ◽  
Mrna Electroporation

Single-Step Antigen Loading and Activation of Dendritic Cells by mRNA Electroporation for the Purpose of Therapeutic Vaccination in Melanoma Patients

2009 ◽  
Vol 15 (10) ◽  
pp. 3366-3375 ◽  
Author(s):  
Aude Bonehill ◽  
An M.T. Van Nuffel ◽  
Jurgen Corthals ◽  
Sandra Tuyaerts ◽  
Carlo Heirman ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Single Step ◽  
Therapeutic Vaccination ◽  
Melanoma Patients ◽  
Mrna Electroporation

Monocyte-derived dendritic cells of patients with coronary artery disease show an increased expression of costimulatory molecules CD40, CD80 and CD86 in vitro

2007 ◽  
Vol 18 (7) ◽  
pp. 523-531 ◽  
Author(s):  
Jörn F. Dopheide ◽  
Urban Sester ◽  
Axel Schlitt ◽  
Georg Horstick ◽  
Hans J. Rupprecht ◽  
...  
Keyword(s):  
Coronary Artery Disease ◽  
Dendritic Cells ◽  
Coronary Artery ◽  
Costimulatory Molecules ◽  
Artery Disease

scholarly journals Expression of a Broad Array of Negative Costimulatory Molecules and Blimp-1 in T Cells following Priming by HIV-1 Pulsed Dendritic Cells

2010 ◽  
Vol 17 (3-4) ◽  
pp. 229-240 ◽  
Author(s):  
Esaki Muthu Shankar ◽  
Karlhans Fru Che ◽  
Davorka Messmer ◽  
Jeffrey D. Lifson ◽  
Marie Larsson
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Costimulatory Molecules ◽  
Broad Array ◽  
Hiv 1

Efficient Expression of the Tumor-Associated Antigen MAGE-3 in Human Dendritic Cells, Using an Avian Influenza Virus Vector

2000 ◽  
Vol 11 (16) ◽  
pp. 2207-2218 ◽  
Author(s):  
Isolde Strobel ◽  
Markus Krumbholz ◽  
Annette Menke ◽  
Erich Hoffmann ◽  
P. Rod Dunbar ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Virus Vector ◽  
Tumor Associated Antigen ◽  
Efficient Expression ◽  
Human Dendritic Cells

scholarly journals The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Jiemin Ding ◽  
Min Liu ◽  
Zihua Xuan ◽  
Meng li Liu ◽  
Ning Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Dendritic Cells ◽  
Ethyl Acetate ◽  
B Cell ◽  
Adjuvant Arthritis ◽  
Ethyl Acetate Fraction ◽  
Costimulatory Molecules ◽  
Inflammatory Factor ◽  
Protective Effects

Aims. The aim of this study was to evaluate the protective effects of Er Miao San (EMS) and the regulative function of bone marrow-derived dendritic cells (BMDCs) on adjuvant arthritis (AA) in rats. Methods. The ethyl acetate part of EMS (3 g/kg, 1.5 g/kg, and 0.75 g/kg) was orally administered from day 15 after immunization to day 29. The polyarthritis index and paw swelling were measured, the ankle joint pathological changes were observed using hematoxylin-eosin (HE) staining, and the spleen and thymus index were determined. Moreover, T and B cell proliferation were determined using the CCK-8 assay. The expression of BMDC surface costimulatory molecules and inflammatory factors were determined using flow cytometry and ELISA kits, respectively. Results. Compared with the AA model rats, the ethyl acetate fraction of EMS obviously reduced paw swelling (from 1.0 to 0.7) and the polyarthritis index (from 12 to 9) P < 0.01 and improved the severity of histopathology P < 0.01 . The treatment using ethyl acetate fraction of EMS significantly reduced the spleen and thymus index P < 0.01 and inhibited T and B cell proliferation P < 0.01 . Moreover, EMS significantly modulated the expression of surface costimulatory molecules in BMDCs, including CD40, CD80, CD86, and major histocompatibility complex class II (MHC-II) P < 0.01 . The results also showed that the ethyl acetate part of EMS significant inhibited the levels of proinflammatory cytokines interleukin- (IL-) 23 tumor necrosis factor- (TNF-) α and inflammatory factor prostaglandin (PG) E2 in the supernatant of BMDCs. However, the level of anti-inflammatory cytokine IL-10 was significantly increased P < 0.01 . Conclusion. These results suggest that the ethyl acetate part of EMS has better protective effects on AA rats, probably by regulating the function of BMDCs and modulating the balance of cytokines.

scholarly journals Resiquimod-Mediated Activation of Plasmacytoid Dendritic Cells Is Amplified in Multiple Sclerosis

2019 ◽  
Vol 20 (11) ◽  
pp. 2811 ◽  
Author(s):  
Marta Corsetti ◽  
Gabriella Ruocco ◽  
Serena Ruggieri ◽  
Claudio Gasperini ◽  
Luca Battistini ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Dendritic Cells ◽  
Viral Infection ◽  
Adaptive Immune Response ◽  
Plasmacytoid Dendritic Cells ◽  
Costimulatory Molecules ◽  
Strong Impact ◽  
Myeloid Dendritic Cells ◽  
Healthy Donors ◽  
Environmental Causes

Background: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease of the central nervous system. The cause of multiple sclerosis is unknown but there are several evidences that associate the genetic basis of the disease with environmental causes. An important association between viral infection and development of MS is clearly demonstrated. Viruses have a strong impact on innate immune cells. In particular, myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs), are able to respond to viruses and to activate the adaptive immune response. Methods: In this study we mimic viral infection using synthetic single-strand RNA, Resiquimod, and we compared the response of both DC subsets derived from healthy donors and MS patients by characterizing the expression of costimulatory molecules on the DC surface. Results: We found that pDCs from MS patients express higher levels of OX40-L, HLA-DR, and CD86 than healthy donors. Moreover, we found that blood cells from MS patients and healthy donors upon Resiquimod-stimulation are enriched in a subpopulation of pDCs, characterized by a high amount of costimulatory molecules. Conclusion: Overall, these results indicate that activation of pDCs is enhanced in MS, likely due to a latent viral infection, and that costimulatory molecules expressed on pDCs could mediate a protective response against the viral trigger of autoimmunity.

scholarly journals Role of the p38 MAPK/C/EBPβ Pathway in the Regulation of Phenotype and IL-10 and IL-12 Production by Tolerogenic Bone Marrow-Derived Dendritic Cells

Cells ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 256 ◽  
Author(s):  
Chantal Guindi ◽  
Alexandre Cloutier ◽  
Simon Gaudreau ◽  
Echarki Zerif ◽  
Patrick P. McDonald ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Dna Binding ◽  
P38 Mapk ◽  
Costimulatory Molecules ◽  
Binding Activity ◽  
Gm Csf ◽  
Dna Binding Activity ◽  
Factor C

Dendritic cells (DCs) play a major role in innate and adaptive immunity and self-immune tolerance. Immunogenic versus tolerogenic DC functions are dictated by their levels of costimulatory molecules and their cytokine expression profile. The transcription factor C/EBPβ regulates the expression of several inflammatory genes in many cell types including macrophages. However, little is known regarding the role of C/EBPβ in tolerogenic versus immunogenic DCs functions. We have previously reported that bone marrow-derived DCs generated with GM-CSF (GM/DCs) acquire the signature of semi-mature tolerogenic IL-10-producing DCs as opposed to immunogenic DCs generated with GM-CSF and IL-4 (IL-4/DCs). Here, we show that tolerogenic GM/DCs exhibit higher levels of phosphorylation and enhanced DNA binding activity of C/EBPβ and CREB than immunogenic IL-4/DCs. We also show that the p38 MAPK/CREB axis and GSK3 play an important role in regulating C/EBPβ phosphorylation and DNA binding activity. Inhibition of p38 MAPK in GM/DCs resulted in a drastic decrease of C/EBPβ and CREB DNA binding activities, a reduction of their IL-10 production and an increase of their IL-12p70 production, a characteristic of immunogenic IL-4/DCs. We also present evidence that GSK3 inhibition in GM/DCs reduced C/EBPβ DNA binding activity and increased expression of costimulatory molecules in GM/DCs and their production of IL-10. Analysis of GM/DCs of C/EBPβ−/− mice showed that C/EBPβ was essential to maintain the semimature phenotype and the production of IL-10 as well as low CD4+ T cell proliferation. Our results highlight the importance of the p38MAPK-C/EBPβ pathway in regulating phenotype and function of tolerogenic GM/DCs.

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