A High Performance Biomarker Detection Method for Exhaled Breath Mass Spectrometry Data

2014 ◽  
pp. 207-216
Author(s):  
Ariadni Papana Dagiasis ◽  
Yuping Wu ◽  
Raed A. Dweik ◽  
David van Duin
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Detection Method ◽  
Mass Spectrometry Data ◽  
Exhaled Breath ◽  
Biomarker Detection

scholarly journals A High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) Qualitative Detection Method Developed for In Vivo Analyses of Toxin Orellanine from the Cortinarius orellanus Fr.—Part II

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Ilia Brondz ◽  
Anton Brondz
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Detection Method ◽  
Stomach Contents ◽  
Laboratory Animals ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Cortinarius Orellanus

The high-performance liquid chromatography—mass spectrometry (HPLC-MS) qualitative analysis method in negative mode of detection was used to record the presence of orellanine in the stomach contents of rats after ingestion of Cortinarius orellanus. Intoxication with orellanine causes acute renal failure (ARF), which is characterized by sudden loss of the ability of the kidneys to excrete wastes. The detection method presented here can be used as a platform for future development of analytical procedures for detecting orellanine in cases of human intoxication by toadstools. The presence of orellanine in animal stomach contents was analytically recorded and compared with standard orellanine, which was donated by the R&D Department of Jupiter Ltd, Norway. The MS fragmentation described in the literature and the MS fragmentation of standard samples of isolated orellanine verified the presence of orellanine in the stomach contents of laboratory animals. The presence of a diglucoside of orellanine, which was described as part of the toxic orellanine complex by Spiteller et al., in the stomach contents of animals, was also verified using HPLC-MS.

scholarly journals Screening Mold Colonies by Using Two Toxicity Assays Revealed Indoor Strains of Aspergillus calidoustus Producing Ophiobolins G and K

Toxins ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 683 ◽  
Author(s):  
Marja Johanna Salo ◽  
Tamás Marik ◽  
Ottó Bencsik ◽  
Raimo Mikkola ◽  
László Kredics ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Proliferation ◽  
Sperm Motility ◽  
High Performance ◽  
Biological Activities ◽  
Membrane Integrity ◽  
Opportunistic Pathogen ◽  
Mass Spectrometry Data ◽  
Toxic Substances ◽  
Boar Sperm

The occurrence and toxin production of the opportunistic pathogen Aspergillus calidoustus in Finnish buildings is not well documented in the literature. We tracked and identified four A. calidoustus colonies cultivated from indoor settled dusts and revealed the biological activities of crude biomass extracts. The toxic substances were identified as 6-epi-ophiobolin K, ophiobolin K, and ophiobolin G by high-performance liquid chromatography–mass spectrometry (HPLC-MS) based on chromatographic and mass spectrometry data (MS and MS/MS) on the crude extract of A. calidoustus strain MH34. A total of 29 fungal colonies collected from settled dust in an office room reported for indoor-air-related illnesses were screened for toxins that inhibited boar sperm motility in the BSMI (boar sperm motility inhibiting) assay and cell proliferation in the ICP (inhibition of cell proliferation) assays with PK-15 cells. Out of the 27 colonies tested as toxic, 12 colonies exhibiting conidiophores representative of the genera Chaetomium, Penicillium, and Paecilomyces were excluded from the study, while 13 colonies exhibited Aspergillus-like conidiophores. Biomass suspensions of these colonies were divided into two categories: Category 1 colonies (n = 4), toxic in the BSMI assay and the ICP assays, emitted blue fluorescence and grew at 37 °C; Category 2 colonies (n = 9), only toxic in the ICP assay, emitted orange fluorescence and exhibited limited or no growth at 37 °C. Colonies in Category 1 were pure-cultured, and the strains were named as MH4, MH21, MH34, MH36. Strain MH34 was identified as A. calidoustus by the internal transcribed spacer (ITS) sequences. Ethanol-soluble dry substances extracted from the biomass of the pure cultures exhibited a toxicological profile in the BSMI assay, SMID (sperm membrane integrity damage) assay, and ICP assay similar to that exhibited by pure ophiobolin A. Overall, the viable conidia of A. calidoustus in indoor settled dusts deserve attention when potentially hazardous mold species are monitored.

scholarly journals Principal Component Analysis from Mass Spectrometry Data Combined to a Sensory Evaluation as a Suitable Method for Assessing Bitterness of Enzymatic Hydrolysates Produced from Micellar Casein Proteins

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1354
Author(s):  
Dahlia Daher ◽  
Barbara Deracinois ◽  
Alain Baniel ◽  
Elodie Wattez ◽  
Justine Dantin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Principal Component Analysis ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Liquid Fraction ◽  
Principal Component ◽  
Component Analysis ◽  
Mass Spectrometry Data ◽  
Sensory Panel ◽  
Enzymatic Hydrolysates

Enzymatic hydrolysis of food proteins generally changes the techno-functional, nutritional, and organoleptic properties of hydrolyzed proteins. As a result, protein hydrolysates have an important interest in the food industries. However, they tend to be characterized by a bitter taste and some off-flavors, which limit their use in the food industry. These tastes and aromas come from peptides, amino acids, and volatile compounds generated during hydrolysis. In this article, sixteen more or less bitter enzymatic hydrolysates produced from a milk protein liquid fraction enriched in micellar caseins using commercially available, food-grade proteases were subjected to a sensory analysis using a trained and validated sensory panel combined to a peptidomics approach based on the peptide characterization by reverse-phase high-performance liquid chromatography, high-resolution mass spectrometry, and bioinformatics software. The comparison between the sensory characteristics and the principal components of the principal component analysis (PCA) of mass spectrometry data reveals that peptidomics constitutes a convenient, valuable, fast, and economic intermediate method to evaluating the bitterness of enzymatic hydrolysates, as a trained sensory panel can do it.

scholarly journals UHPLC Analysis of Saffron (Crocus sativus L.): Optimization of Separation Using Chemometrics and Detection of Minor Crocetin Esters

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1851 ◽  
Author(s):  
Angelo D’Archivio ◽  
Francesca Di Donato ◽  
Martina Foschi ◽  
Maria Maggi ◽  
Fabrizio Ruggieri
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Mass Spectrometry Data ◽  
Crocus Sativus ◽  
Column Temperature ◽  
Optimal Separation ◽  
Crocus Sativus L ◽  
Separation Conditions

Ultra-high performance liquid chromatography (UHPLC) coupled with diode array detection (DAD) was applied to improve separation and detection of mono- and bis-glucosyl esters of crocetin (crocins), the main red-colored constituents of saffron (Crocus sativus L.), and other polar components. Response surface methodology (RSM) was used to optimise the chromatographic resolution on the Kinetex C18 (Phenomenex) column taking into account of the combined effect of the column temperature, the eluent flow rate and the slope of a linear eluent concentration gradient. A three-level full-factorial design of experiments was adopted to identify suitable combinations of the above factors. The influence of the separation conditions on the resolutions of 22 adjacent peaks was simultaneously modelled by a multi-layer artificial neural network (ANN) in which a bit string representation was used to identify the target analytes. The chromatogram collected under the optimal separation conditions revealed a higher number of crocetin esters than those already characterised by means of mass-spectrometry data and usually detected by HPLC. Ultra-high performance liquid chromatography analyses carried out on the novel Luna Omega Polar C18 (Phenomenex) column confirmed the large number of crocetin derivatives. Further work is in progress to acquire mass-spectrometry data and to clarify the chemical structure to the newly found saffron components.

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