Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation

2016 ◽  
pp. 161-177 ◽  
Author(s):  
Kasper Engholm-Keller ◽  
Martin R. Larsen
Keyword(s):  
Phosphopeptide Enrichment ◽  
Limited Sample

The Oxford Handbook of the Operatic Canon

2020 ◽  
Keyword(s):  
Musical Theater ◽  
Geographical Location ◽  
The United States ◽  
Late Nineteenth Century ◽  
Limited Sample ◽  
The United Kingdom ◽  
Production And Consumption ◽  
Formation History ◽  
Artistic Directors ◽  
Selection Of

This collection examines the phenomenon of the operatic canon: its formation, history, current ontology and practical influence, and future. It does so by taking an international and interdisciplinary view: the workshops from which it was derived included the participation of critics, producers, artistic directors, stage directors, opera company CEOs, and even economists, from the United Kingdom, the United States, France, Italy, Ireland, Germany, the Netherlands, Denmark, and Canada. The volume is structured as a series of dialogues: each subtopic is addressed by two essays, introduced jointly by the authors, and followed by a jointly compiled list of further reading. These paired essays complement each other in different ways, for example by treating the same geographical location in different periods, by providing different national or regional perspectives on the same period, or by thinking through similar conceptual issues in contrasting milieus. Part I consists of a selection of surveys of operatic production and consumption contexts in France, Italy, Germany, England, Russia, and the Americas, arranged in rough order from the late seventeenth century to the late nineteenth century. Part II is a (necessarily) limited sample of subjects that illuminate the operatic canon from different—sometimes intentionally oblique—angles, ranging from the influence of singers to the contiguous genres of operetta and musical theater, and the effects of recording and broadcast over almost 150 years. The volume concludes with two essays written by prominent figures from the opera industry who give their sense of the operatic canon’s evolution and prospects.

scholarly journals Bayesian graphical models for modern biological applications

2021 ◽  
Author(s):  
Yang Ni ◽  
Veerabhadran Baladandayuthapani ◽  
Marina Vannucci ◽  
Francesco C. Stingo
Keyword(s):  
Graphical Models ◽  
Cancer Genomics ◽  
Graph Structure ◽  
Biological Processes ◽  
Limited Sample ◽  
Large Networks ◽  
Bayesian Approaches ◽  
Complex Sampling ◽  
Complex Dependence ◽  
Bayesian Graphical Models

AbstractGraphical models are powerful tools that are regularly used to investigate complex dependence structures in high-throughput biomedical datasets. They allow for holistic, systems-level view of the various biological processes, for intuitive and rigorous understanding and interpretations. In the context of large networks, Bayesian approaches are particularly suitable because it encourages sparsity of the graphs, incorporate prior information, and most importantly account for uncertainty in the graph structure. These features are particularly important in applications with limited sample size, including genomics and imaging studies. In this paper, we review several recently developed techniques for the analysis of large networks under non-standard settings, including but not limited to, multiple graphs for data observed from multiple related subgroups, graphical regression approaches used for the analysis of networks that change with covariates, and other complex sampling and structural settings. We also illustrate the practical utility of some of these methods using examples in cancer genomics and neuroimaging.

scholarly journals P092 NICE biologics pathways for inflammatory arthritis exhibit regional variability due to modification by CCG’s: results from a national survey of pathways in England

Rheumatology ◽  
2021 ◽  
Vol 60 (Supplement_1) ◽  
Author(s):  
Jatin Mistry ◽  
Diane Hill ◽  
Ailsa Bosworth ◽  
Arvind Kaul
Keyword(s):  
Inflammatory Arthritis ◽  
Good Practice ◽  
National Study ◽  
Freedom Of Information ◽  
Inadequate Response ◽  
Limited Sample ◽  
Regional Variability ◽  
Nice Guidance ◽  
Information Requests ◽  
Individual Funding

Abstract Background/Aims  NICE publishes guidance underpinned by act of Parliament and legally enforceable, on the use of biological therapies in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PsA) and ankylosing spondylitis (AS) which should allow harmonisation of access independent of region. However, sufficient guidance is not provided on the use of sequential biologics nor is a numerical cap placed on the number of biologics a patient can attempt if they have had an inadequate response. We have previously reported that in a limited sample, Clinical Commissioning Groups (CCGs) interpret NICE guidance variably and restrict access to NICE approved treatments depending on geography, the so-called “postcode lottery”. We determined the variability of biologics pathways in all CCG’s in England to examine whether a potentially unfair postcode lottery exists for sequential biologics use. Methods  All 135 England CCGs covering over 55 million people, were sent Freedom of Information requests, for their biologic pathways for RA, PsA and AS. Where CCGs did not have this information, the relevant acute trusts were contacted, with responses recorded under that CCG. For every CCG the local biologics pathways were examined for detail on the number and type of biologics commissioned before an Individual Funding Request was needed. “No Cap” was recorded if CCG’s responded with no restriction on the number of biologics. Results  Responses were obtained from 124/135 CCG’s for RA, 122/135 for PsA and AS, all covering an estimated population in excess of 45 million people. For RA, 55% CCG’s had no cap on the number of commissioned RA biologics. 45% had a variable cap from 3 to 6 commissioned biologics. For PsA, the figures were 54% with no cap and 46% with variable capping between 2-5 biologics allowed, for AS the figures were 51% and 49% respectively. In total this represented 41 different local pathways for RA, 29 different pathways for PsA and in AS where fewer biologics choices exist, 25 different pathways depending on CCG and location. Conclusion  There is wide regional variation in the interpretation of NICE guidance by CCG’s resulting in many different local pathways depending on geography. Approximately 50% of pathways restricted biologics prescribing by mandating the type and sequence of biologics used, potentially compromising patient care and delaying treatment by requiring an IFR for a NICE approved biologic. Moreover, pathways varied as to which biologics could be used at any point of management by region as well. As exemplars of good practice, approximately 50% of CCG’s had no cap, allowing clinical freedom to prescribe the most appropriate biologic. The results of this national study demonstrate the variability of biologics pathways in many areas of England ensuring a postcode lottery still exists in many regions. Disclosure  J. Mistry: None. D. Hill: None. A. Bosworth: None. A. Kaul: None.

Phytic acid functionalized magnetic bimetallic metal–organic frameworks for phosphopeptide enrichment

2021 ◽  
Vol 9 (7) ◽  
pp. 1811-1820
Author(s):  
Shuang Yan ◽  
Bin Luo ◽  
Jia He ◽  
Fang Lan ◽  
Yao Wu
Keyword(s):  
Phytic Acid ◽  
Efficient Method ◽  
High Selectivity ◽  
Metal Organic Framework ◽  
Metal Organic Frameworks ◽  
High Sensitivity ◽  
Phosphopeptide Enrichment ◽  
Metal Organic ◽  
Organic Framework

Novel bimetallic metal–organic framework nanocomposites were fabricated by a facile yet efficient method. The as-prepared nanomaterial exhibited high sensitivity and high selectivity toward phosphopeptides and good reusability of five cycles for enriching phosphopeptides.

scholarly journals Association between triglycerides, known risk SNVs and conserved rare variation in SLC25A40 in a multi-ancestry cohort

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Elisabeth A. Rosenthal ◽  
David R. Crosslin ◽  
Adam S. Gordon ◽  
David S. Carrell ◽  
Ian B. Stanaway ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Large Family ◽  
Missense Variant ◽  
Mixed Ancestry ◽  
Limited Sample ◽  
Health Records ◽  
Pathogenic Variants ◽  
Phenotype Data ◽  
Emerge Network ◽  
Using Data

Abstract Background Elevated triglycerides (TG) are associated with, and may be causal for, cardiovascular disease (CVD), and co-morbidities such as type II diabetes and metabolic syndrome. Pathogenic variants in APOA5 and APOC3 as well as risk SNVs in other genes [APOE (rs429358, rs7412), APOA1/C3/A4/A5 gene cluster (rs964184), INSR (rs7248104), CETP (rs7205804), GCKR (rs1260326)] have been shown to affect TG levels. Knowledge of genetic causes for elevated TG may lead to early intervention and targeted treatment for CVD. We previously identified linkage and association of a rare, highly conserved missense variant in SLC25A40, rs762174003, with hypertriglyceridemia (HTG) in a single large family, and replicated this association with rare, highly conserved missense variants in a European American and African American sample. Methods Here, we analyzed a longitudinal mixed-ancestry cohort (European, African and Asian ancestry, N = 8966) from the Electronic Medical Record and Genomics (eMERGE) Network. We tested associations between median TG and the genes of interest, using linear regression, adjusting for sex, median age, median BMI, and the first two principal components of ancestry. Results We replicated the association between TG and APOC3, APOA5, and risk variation at APOE, APOA1/C3/A4/A5 gene cluster, and GCKR. We failed to replicate the association between rare, highly conserved variation at SLC25A40 and TG, as well as for risk variation at INSR and CETP. Conclusions Analysis using data from electronic health records presents challenges that need to be overcome. Although large amounts of genotype data is becoming increasingly accessible, usable phenotype data can be challenging to obtain. We were able to replicate known, strong associations, but were unable to replicate moderate associations due to the limited sample size and missing drug information.

Facile synthesis of Ti4+-immobilized magnetic covalent organic frameworks for enhanced phosphopeptide enrichment

Talanta ◽  
2021 ◽  
pp. 122789
Author(s):  
Yanting He ◽  
Shasha Zhang ◽  
Chao Zhong ◽  
Yixin Yang ◽  
Guorong Li ◽  
...  
Keyword(s):  
Covalent Organic Frameworks ◽  
Phosphopeptide Enrichment ◽  
Facile Synthesis

scholarly journals The Metallicity Distribution of Late Type Dwarfs

1998 ◽  
Vol 11 (1) ◽  
pp. 571-571
Author(s):  
M. Haywood ◽  
J. Palasi ◽  
A. Gómez ◽  
L. Meillon Dasgal
Keyword(s):  
Star Formation Rate ◽  
Formation Rate ◽  
Angular Separation ◽  
Stellar Populations ◽  
Late Type ◽  
Limited Sample ◽  
Hipparcos Catalogue ◽  
Galactic Stellar Populations ◽  
Metallicity Distribution ◽  
Hr Diagram

The Hipparcos catalogue provides an accurate and extensive sampling of the solar neighbourhood HR diagram. The morphology of this diagram depends on selection criteria of the catalogue such as the limiting magnitude, angular separation and on the characteristics of the stellar populations near the sun (space density, metallicity, star formation rate, etc). Since the Hipparcos data are so accurate, one needs to model precisely the different selection bias and, at the same time, parametrize models of the galactic stellar populations with sufficient flexibility that as much information as possible can be grasped from the catalogue. Comparisons between our model and the Hipparcos catalogue will be presented elsewhere. Since the quantity of information contained in the Hipparcoscatalogue is so important, models ought to be complex, and external contraints, obtained prior to any general comparison with the model, are welcome. A major factor that influences the distribution of the stars in the HR diagram is the metallicity. For the late type stars, the metallicity distribution can be best studied by re-analysing a volume-limited sample of stars from the catalogue.

scholarly journals Data Augmentation and Spectral Structure Features for Limited Samples Hyperspectral Classification

2021 ◽  
Vol 13 (4) ◽  
pp. 547
Author(s):  
Wenning Wang ◽  
Xuebin Liu ◽  
Xuanqin Mou
Keyword(s):  
Classification Accuracy ◽  
Data Augmentation ◽  
Classification Problem ◽  
Classification Performance ◽  
Spectral Structure ◽  
Limited Sample ◽  
Sample Classification ◽  
Training Samples ◽  
Traditional Classification ◽  
Hyperspectral Classification

For both traditional classification and current popular deep learning methods, the limited sample classification problem is very challenging, and the lack of samples is an important factor affecting the classification performance. Our work includes two aspects. First, the unsupervised data augmentation for all hyperspectral samples not only improves the classification accuracy greatly with the newly added training samples, but also further improves the classification accuracy of the classifier by optimizing the augmented test samples. Second, an effective spectral structure extraction method is designed, and the effective spectral structure features have a better classification accuracy than the true spectral features.

scholarly journals Limited Bilateral Advancement of the Sternocostal Head of Pectoralis Major for Sternal Reconstruction: Preserving the Axillary Fold

2017 ◽  
Vol 102 (3-4) ◽  
pp. 189-195
Author(s):  
Warren M. Rozen ◽  
Ken G. W. Teo ◽  
Gausihi Sivarajah ◽  
Rafael Acosta
Keyword(s):  
Pectoralis Major ◽  
High Rate ◽  
Pectoralis Major Flap ◽  
Limited Sample ◽  
Wound Coverage ◽  
Caudal Portion ◽  
Sternal Reconstruction ◽  
The Right ◽  
Good Cosmesis ◽  
Rate Of Success

The introduction of well-vascularized flaps for infected sternotomy wound reconstruction has improved mortality rates dramatically. Multiple variations of the pectoralis major flap have been described in this context. However, unresolved limitations of this flap include poor cosmesis and problematic coverage of the inferior third of the sternotomy wound. We describe an approach to address these issues. The humeral attachments are preserved and bilateral muscles are advanced in a limited fashion. The left sternocostal head is advanced medially and rotated anticlockwise, using this portion to fill the upper half of the sternum while the caudal portion of the right pectoralis muscle is used as a turnover flap at the lower half of the wound. In all 25 patients, the anterior axillary fold was preserved bilaterally and the infection completely resolved. Complications included 3 cases of hematoma, 2 cases of coagulopathy, and 1 late bone sequestrum (aseptic). Although the study had a limited sample size, we had a high rate of success and few complications. With the preservation of bilateral axillary folds, good cosmesis, and adequate wound coverage, we recommend this modification of the pectoralis major flap in even complicated cases of mediastinitis.

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