scholarly journals STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells

2017 ◽  
pp. 65-78 ◽  
Author(s):  
Roman S. Erdmann ◽  
Derek Toomre ◽  
Alanna Schepartz
Keyword(s):  
High Density ◽  
Live Cells ◽  
Two Component ◽  
High Density Lipid ◽  
Lipid Probe

scholarly journals A protein–dye hybrid system as a narrow range tunable intracellular pH sensor

2016 ◽  
Vol 7 (11) ◽  
pp. 6808-6814 ◽  
Author(s):  
Palapuravan Anees ◽  
Karivachery V. Sudheesh ◽  
Purushothaman Jayamurthy ◽  
Arunkumar R. Chandrika ◽  
Ramakrishnapillai V. Omkumar ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Hybrid System ◽  
Narrow Range ◽  
Ph Sensor ◽  
Organic Dye ◽  
Live Cells ◽  
Two Component ◽  
Ph Variations

A two-component hybrid system consisting of organic dye nanoparticles (ash) and a protein (blue) at different ratios allows for the design of narrow range tunable nanoprobes for sensing and imaging of pH variations in live cells through ratiometric signalling.

scholarly journals Controlling the Behavior of Single Live Cells with High Density Arrays of Microscopic OLEDs

2015 ◽  
Vol 27 (46) ◽  
pp. 7657-7661 ◽  
Author(s):  
Anja Steude ◽  
Matthias Jahnel ◽  
Michael Thomschke ◽  
Matthias Schober ◽  
Malte C. Gather
Keyword(s):  
High Density ◽  
Live Cells

scholarly journals Bistability of a coupled Aurora B kinase-phosphatase system in cell division

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Anatoly V Zaytsev ◽  
Dario Segura-Peña ◽  
Maxim Godzi ◽  
Abram Calderon ◽  
Edward R Ballister ◽  
...  
Keyword(s):  
Cell Division ◽  
Regulatory Mechanisms ◽  
Live Cells ◽  
Aurora B ◽  
Aurora B Kinase ◽  
Bistable Behavior ◽  
Spatial Regulation ◽  
Two Component ◽  
Phosphatase System ◽  
Kinase A

Aurora B kinase, a key regulator of cell division, localizes to specific cellular locations, but the regulatory mechanisms responsible for phosphorylation of substrates located remotely from kinase enrichment sites are unclear. Here, we provide evidence that this activity at a distance depends on both sites of high kinase concentration and the bistability of a coupled kinase-phosphatase system. We reconstitute this bistable behavior and hysteresis using purified components to reveal co-existence of distinct high and low Aurora B activity states, sustained by a two-component kinase autoactivation mechanism. Furthermore, we demonstrate these non-linear regimes in live cells using a FRET-based phosphorylation sensor, and provide a mechanistic theoretical model for spatial regulation of Aurora B phosphorylation. We propose that bistability of an Aurora B-phosphatase system underlies formation of spatial phosphorylation patterns, which are generated and spread from sites of kinase autoactivation, thereby regulating cell division.

scholarly journals Two-Component Sensor Required for Normal Symbiotic Colonization of Euprymna scolopes by Vibrio fischeri

2001 ◽  
Vol 183 (3) ◽  
pp. 835-842 ◽  
Author(s):  
Karen L. Visick ◽  
Line M. Skoufos
Keyword(s):  
Sequence Analysis ◽  
Vibrio Fischeri ◽  
Response Regulator ◽  
High Density ◽  
Membrane Topology ◽  
Symbiotic Association ◽  
Light Organ ◽  
Euprymna Scolopes ◽  
Putative Promoter Region ◽  
Two Component

ABSTRACT The light organ of the squid Euprymna scolopes is specifically colonized to a high density by the marine bacteriumVibrio fischeri. To date, only a few factors contributing to the specificity of this symbiosis have been identified. Using a genetic screen for random transposon mutants defective in initiating the symbiotic association or in colonizing the light organ to high density, we identified a mutant of V. fischeri that exhibited an apparent defect in symbiosis initiation. This mutant was not defective in motility, luminescence, or growth in minimal medium, suggesting that it lacks an essential, previously unidentified symbiotic function. By sequence analysis, we showed that the locus inactivated in this mutant encodes a predicted 927-amino-acid protein with a high degree of similarity to the sensor component of hybrid two-component regulatory systems. We have therefore designated this locus rscS, for regulator of symbiotic colonization—sensor. Sequence analysis revealed two hydrophobic regions which may result in the formation of a periplasmic loop involved in signal recognition; PhoA fusion data supported this proposed membrane topology. We have investigated the start site ofrscS transcription by primer extension and identified a putative promoter region. We hypothesize that RscS recognizes a signal associated with the light organ environment and responds by stimulating a putative response regulator that controls protein function or gene expression to coordinate early colonization events. Further studies on RscS, its cognate response regulator, and the signaling conditions will provide important insight into the interaction between V. fischeri and E. scolopes.

Initial Hypoalbuminemia and Hyperlipidemia Persist during Chronic Peritoneal Dialysis in Children

1993 ◽  
Vol 13 (2) ◽  
pp. 136-139 ◽  
Author(s):  
Dennis Scolnik ◽  
J. Williamson Balfe
Keyword(s):  
Peritoneal Dialysis ◽  
Serum Lipids ◽  
High Density ◽  
Therapeutic Interventions ◽  
Cholesterol Levels ◽  
High Incidence ◽  
Continuous Cycling ◽  
Low Levels ◽  
Average Serum ◽  
High Density Lipid

Objective To document serum lipid values in pediatric peritoneal dialysis (PD) patients, particularly the very young, and investigate relationships with serum albumin levels. Design Retrospective review of all PD patients seen in the first 11 years of the PD program at our institution. Patients and Methods Any pediatric PD patient was eligible for inclusion if at least four simultaneous measurements of serum lipids and albumin were recorded over a minimum of 90 days of PD. Results Thirty-nine continuous ambulatory peritoneal dialysis (CAPD) patients (9, aged <5 years) and 14 continuous cycling peritoneal dialysis (CCPD) patients (7, aged <5 years) were followed for 90–1200 days. Hypoalbuminemia, present In 43% of initial recordings, did not alter significantly during PD in any group; it was most marked in the CAPD <5 year (mean 30.7 g/L) and CCPD >5 year groups (mean 31.4 g/L). Average serum cholesterol levels were 27% higher, and triglyceride 122% higher, than the maximum accepted 18-year-old level and did not change significantly during PD. Similarly, 33% of high-density lipid recordings were below normal and remained unaltered during PD. Conclusions Our results confirm the high incidence of hypoalbuminemia, hypertriglyceridemia, and hypercholesterolemia and associated low levels of high density lipids, even in young PD patients, and demonstrate that these remain unchanged during PD. Such results have not previously been reported in PD patients as young as ours. Abnormal lipid profiles are a neglected cardiovascular risk factor in children with renal failure; therapeutic interventions should therefore be seriously considered.

scholarly journals Development of a Prognostic Model for Stump Healing in Major Lower Limb Amputation among the Diabetic Population

Diabetology ◽  
2021 ◽  
Vol 2 (3) ◽  
pp. 130-140
Author(s):  
Suhel Ashraff ◽  
Muhammad Siddiqui ◽  
Thomas Carline ◽  
Robert Rush ◽  
Derek Santos ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Serum Sodium ◽  
Lower Limb ◽  
Prognostic Model ◽  
Normal Serum ◽  
High Density ◽  
Lower Limb Amputation ◽  
Limb Amputation ◽  
Goodness Of Fit Test ◽  
High Density Lipid

Background: This study aimed to explore the effect of haematological markers as well as patient characteristics on stump healing in patients who underwent a lower limb amputation procedure. In addition, a practical model regarding factors that affected stump healing was developed. Methods: Patients who underwent a major lower limb amputation (above knee and below knee) at the Royal Infirmary of Edinburgh from the period of 2007 to 2010 were included in this study. A prognostic model utilizing backward stepwise logistical regression was developed to measure the probability of lower limb stump healing. The relationship between the dependent and independent variables was identified using univariate and multivariate logistic regression. Results: Three variables, namely serum sodium, serum creatinine and serum high density lipid cholesterol were identified which influenced stump healing. Patients with normal serum sodium were 75% more likely to have lower limb stump healing compared to that of patients with abnormal serum sodium (odds ratio [OR] 1.756; 95% confidence interval [CI] 1.048–2.942). Patients with normal serum creatinine were 66% more likely to have their stump healed (OR 1.664; 95% CI 0.94 to 2.946). The healing rate of patients with a normal level of serum high density lipid cholesterol was 75%, in contrast to patients with an aberrant level of serum high density lipids cholesterol (OR 1.753; 95% CI 1.061 to 2.895). The effectiveness of the retrospective stump-healing model was demonstrated by the area under the ROC curve (0.612), which was supported by the Hosmer and Lemeshow goodness-of-fit test (p = 0.879). Conclusions: Serum sodium, serum high density lipid cholesterol and serum creatinine have a strong correlation with lower limb stump healing. However, serum sodium and serum high density lipid cholesterol secondary to multiple co-morbidities in this cohort group could be altered secondary to disease pathology itself.

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