Bioseparation of Recombinant Proteins from Plant Extract with Hydrophobin Fusion Technology

2011 ◽  
pp. 527-534 ◽  
Author(s):  
Jussi J. Joensuu ◽  
Andrew J. Conley ◽  
Markus B. Linder ◽  
Rima Menassa
Keyword(s):  
Recombinant Proteins ◽  
Plant Extract ◽  
Fusion Technology

scholarly journals Study on the transient expression of hemagglutinin antigen of influenza A/H7N9 virus in Nicotiana benthamiana using agro-infiltration

2018 ◽  
Vol 15 (1) ◽  
pp. 133-140
Author(s):  
Lê Thị Thủy ◽  
Lê Thu Ngọc ◽  
Hồ Thị Thương ◽  
Phạm Bích Ngọc ◽  
Lâm Đại Nhân ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Transient Expression ◽  
Recombinant Proteins ◽  
Plant Extract ◽  
Blood Cells ◽  
Nicotiana Benthamiana ◽  
Influenza A ◽  
Total Soluble Protein ◽  
Hemagglutinin Protein ◽  
Ha Protein

Avian influenza A/H7N9 is a subtype of influenza viruses that has been detected in birds in the past. This subtype has not been previously seen in either animals or human until it was found in March 2013 in China. However, since then, infections of modified H7N9 in both human and birds have been observed such as H7N2, H7N3 and H7N5 subtypes. Hemagglutinin (HA) is the most important antigen for induction of immunity, and is a target to develop influenza vaccines. In this study, the gene encoding HA protein was sucessfully inserted into pRTRA vectors to generate 35S-HA-histag-cmyc-ELP-KDEL and 35S-HApII-histag-cmyc-ELP-KDEL cassettes in the absence and presence of trimer motif GCN4, respectively in order to enhance accumulation of recombinant proteins in plants. These cassettes were inserted into pCB301 binary vectors and transformed into Agrobacterium tumefaciens in order to carry out the transient expression in Nicotiana benthamiana leaves. Five days after infiltration, the total soluble protein was extracted and analysed in SDS-PAGE. The HA-ELP and (HApII-ELP)3 recombinant proteins were detected by Western blot using anti-cmyc monoclonal antibody. These results showed that HA protein fused ELP was observed in monomeric and trimeric forms. The plant extract containing hemagglutinin protein in monomeric and trimeric forms were characterized bio-function using hemagglutination assay. The plant extract containing hemagglutinin protein in trimeric form agglutinated chicken red blood cells at concentration of 94 µg protein/ml whereas the plant extract containing hemagglutinin protein in monomeric form did not agglutinate chicken red blood cells at the same of protein concentration.

High throughput screening to investigate Brazilian Cerrado biome plant extract bank chemical diversity

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
LS Espindola ◽  
RG Dusi ◽  
KR Gustafson ◽  
J McMahon ◽  
JA Beutler
Keyword(s):  
High Throughput ◽  
Plant Extract ◽  
High Throughput Screening ◽  
Chemical Diversity ◽  
Brazilian Cerrado ◽  
Cerrado Biome

Acute renal failure caused by plant extract

2007 ◽  
Vol 148 (9) ◽  
pp. 421-424 ◽  
Author(s):  
Katalin Vágási ◽  
Péter Degrell ◽  
István Késői ◽  
Tibor Kovács ◽  
István Pintér ◽  
...  
Keyword(s):  
Renal Failure ◽  
Acute Renal Failure ◽  
Plant Extract

A szerzők egy 48 órája fennálló anuria miatt vizsgált 30 éves nőbeteg esetét ismertetik, aki tartósan szedett Guarana növényőrleményt tartalmazó készítményt, emellett alkalmanként parenteralis nemszteroid fájdalomcsillapítót kapott. A klinikai kép és a laborvizsgálatok akut máj- és veseelégtelenséget mutattak. Az elvégzett vesebiopszia szövettani képe akut tubularis necrosist igazolt. Átmeneti dialíziskezelést követően kompenzáló polyuria mellett vesefunkciós értékei fokozatosan rendeződtek. A szerzők az esetismertetés kapcsán szeretnék felhívni a figyelmet arra, hogy a gyógyszertárakban és egyéb, gyógyhatású készítményeket, étrendkiegészítőket forgalmazó boltokban kapható szabadforgalmú készítmények némelyikének alkalmazása, illetve a gyógyszerészeti hivatal által törzskönyvezett készítményekkel való együttes alkalmazása súlyos veszélyeket rejthet.

BUBBLE NUCLEAR FUSION TECHNOLOGY-STATUS AND CHALLENGES

2005 ◽  
Vol 17 (3) ◽  
pp. 191-224 ◽  
Author(s):  
R. P. Taleyarkhan ◽  
Richard T. Lahey, Jr. ◽  
Academician R. I. Nigmatulin
Keyword(s):  
Nuclear Fusion ◽  
Fusion Technology

Phytosome Drug Delivery of Natural Products: A Promising Technique for Enhancing Bioavailability

2017 ◽  
Vol 7 (03) ◽  
Author(s):  
Rani Anjana ◽  
Kumar Sunil ◽  
Sharma Hitender ◽  
Khar R. K.
Keyword(s):  
Drug Delivery ◽  
Natural Products ◽  
Plant Extract ◽  
Herbal Extract ◽  
Present Review ◽  
Absorption Profile ◽  
Promising Technique ◽  
Characterization Techniques ◽  
Advanced Form

The phytosome technology was developed by Indena markedly enhancing the bioavailability of selected phytomedicines, by incorporating phospholipids into standardized plant extract, which improve their absorption and utilization. Phytosome are advanced form of herbal extract that shows better absorption profile than conventional herbal extract. The present review focus on the preparation and characterization techniques of phytosomes, merits and various landmarks in the field of phytosomes.

The Protective Efficacy of Ethanolic Leaves Extract of Citronella Gras (Cymbopogon nardus) on Glucose Metabolism Alteration Induced By Mercury in Rats

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Fransisca Diana Alexandra ◽  
Dian Mutiasari ◽  
Trilianty Lestarisa ◽  
Eko Suhartono
Keyword(s):  
Glucose Metabolism ◽  
Protective Effect ◽  
Plant Extract ◽  
Protective Efficacy ◽  
Liver Weight ◽  
Liver Glycogen ◽  
Liver Cells ◽  
Glycogen Level ◽  
Group I ◽  
Citronella Grass

The present study was undertaken to investigate the protective effect of ethanolic citronella grass (C. nardus) leaves extract against mercury (Hg) induced glucose metabolism alteration in rats. Four groups of rats were selected, with 6 rats for each group. Animals of group I was received a 1 ppm of Hg only. Animals of groups II, III, and IV received a combination of 1 ppm Hg and plant extract in different dose (1650, 2520, and 3360 mg/ml). The experiment lasted for 4 weeks. The various parameters studied included liver weight, liver glucose, glycogen, and malondialdehyde (MDA) level in all groups after treatment. The results of this present studies showed that the Hg-induced glucose metabolism alteration in rats which can be seen from the increase of liver glucose and the decreasing of liver glycogen levels. The results also showed that the Hginduced glucose metabolism alteration through its activities in the trigger the liver cells damage which can be seen from the decreasing of liver weight and the increase of liver MDA level. The ethanolic of C. nardus leaves extract shows a protective effect to maintain all parameters into a better a condition which can be seen from the significant increase in liver weight and liver glycogen level, and the significant decrease in liver glucose and MDA levels. The present study indicated that the ethanolic C. nardus leaves extract showed a potential protective effect on glucose metabolism alteration induced by Hg

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