Bacteriological Study of Pseudomonas Aeruginosa Isolated from Different Infections and Study Antimicrobial Activities of Plant Extract Solanum Nigrum Against It

2017 ◽  
Vol 58 (4C) ◽  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Plant Extract ◽  
Antimicrobial Activities ◽  
Solanum Nigrum ◽  
Bacteriological Study

scholarly journals Antimicrobial Activities of Pseudomonads against Plant Pathogenic Organisms and Efficacy of Pseudomonas aeruginosa ATCC7700 against Bacterial Wilt of Tomato.

1997 ◽  
Vol 63 (6) ◽  
pp. 417-424 ◽  
Author(s):  
Naruto FURUYA ◽  
Shuichi YAMASAKI ◽  
Masanori NISHIOKA ◽  
Ikuo SHIRAISHI ◽  
Kazuhiro IIYAMA ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Wilt ◽  
Antimicrobial Activities ◽  
Pathogenic Organisms

scholarly journals The Impact of an Efflux Pump Inhibitor on the Activity of Free and Liposomal Antibiotics against Pseudomonas aeruginosa

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 577
Author(s):  
Douweh Leyla Gbian ◽  
Abdelwahab Omri
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Efflux Pump ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Efflux Pump Inhibitor ◽  
Signal Production ◽  
Lung Infections ◽  
The Impact ◽  
Microbroth Dilution

The eradication of Pseudomonas aeruginosa in cystic fibrosis patients has become continuously difficult due to its increased resistance to treatments. This study assessed the efficacy of free and liposomal gentamicin and erythromycin, combined with Phenylalanine arginine beta-naphthylamide (PABN), a broad-spectrum efflux pump inhibitor, against P. aeruginosa isolates. Liposomes were prepared and characterized for their sizes and encapsulation efficiencies. The antimicrobial activities of formulations were determined by the microbroth dilution method. Their activity on P. aeruginosa biofilms was assessed, and the effect of sub-inhibitory concentrations on bacterial virulence factors, quorum sensing (QS) signals and bacterial motility was also evaluated. The average diameters of liposomes were 562.67 ± 33.74 nm for gentamicin and 3086.35 ± 553.95 nm for erythromycin, with encapsulation efficiencies of 13.89 ± 1.54% and 51.58 ± 2.84%, respectively. Liposomes and PABN combinations potentiated antibiotics by reducing minimum inhibitory and bactericidal concentrations by 4–32 fold overall. The formulations significantly inhibited biofilm formation and differentially attenuated virulence factor production as well as motility. Unexpectedly, QS signal production was not affected by treatments. Taken together, the results indicate that PABN shows potential as an adjuvant of liposomal macrolides and aminoglycosides in the management of lung infections in cystic fibrosis patients.

Ginseng aqueous extract attenuates the production of virulence factors, stimulates twitching and adhesion, and eradicates biofilms of Pseudomonas aeruginosa

2011 ◽  
Vol 89 (6) ◽  
pp. 419-427 ◽  
Author(s):  
Misagh Alipour ◽  
Abdelwahab Omri ◽  
Zacharias E. Suntres
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
Aqueous Extract ◽  
North American ◽  
American Ginseng ◽  
Antimicrobial Activities ◽  
Live Cells ◽  
Dilution Method ◽  
Agar Dilution Method

This study was carried out to examine the antimicrobial activity of the aqueous extract of Panax quinquefolius from North American ginseng (NAGE) root against Pseudomonas aeruginosa . The minimum inhibitory concentrations of reference and clinical isolates of Pseudomonas aeruginosa were measured by a standard agar-dilution method. At subinhibitory NAGE concentrations, the secretion of virulence factors, motility on agar, and adhesion to 96-well microplates were studied on the nonmucoid Pseudomonas aeruginosa O1 strain. At suprainhibitory concentrations, the activity of NAGE against mature biofilm complexes formed in the Calgary Biofilm Device and the Stovall flow cell were assessed. NAGE possessed an antibacterial activity against all the Pseudomonas aeruginosa strains at 1.25%–2.5% w/v. NAGE also significantly attenuated pyocyanin, pyoverdine, and lipase concentrations, stimulated twitching, and attenuated swarming and swimming motility. At 1.25% w/v, NAGE augmented adhesion, and at 5% w/v detached 1-day-old biofilms in microplates. The extract also eradicated 6-day-old mature biofilms (5% w/v), and fluorescence microscopy displayed a reduction of live cells and biofilm complexes compared with nontreated biofilms. These data suggest that the aqueous extract from North American ginseng possesses antimicrobial activities in vitro.

scholarly journals Green synthesis of Ag, Zn and Cu nanoparticles from aqueous extract of Spondias mombin leaves and evaluation of their antibacterial activity

2020 ◽  
Vol 21 (2) ◽  
pp. 106-113
Author(s):  
D.K. Adeyemi ◽  
A.O. Adeluola ◽  
M.J. Akinbile ◽  
O.O. Johnson ◽  
G.A. Ayoola
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Green Synthesis ◽  
Antimicrobial Activities ◽  
Cu Nanoparticles ◽  
E Coli ◽  
Spondias Mombin ◽  
Ft Ir ◽  
Uv Visible

Background: Nanotechnology offers an advantage as a green route for synthesis of metal nanoparticles (NPs) with plant extracts as capping agent. Spondias mombin is a fruit-bearing tree and its leaf extracts have been reported to possess anxiolytic, hypoglycaemic, antiepileptic, antipsychotic, sedative, antioxidant, and antimicrobial properties. The objective of the study is to determine the antibacterial potential of a simple non-toxic product of green synthesis of metallic (Ag, Zn and Cu) nanoparticles using the leaf of Spondias mombin aqueous extracts (SMAE) as a reducing and capping agents of the metal ions.Methodology: Nanoparticles were characterized by UV visible spectrophotometeric analysis, Fourier Transform Infra-Red (FT-IR) spectrophotometer and scanning electron microscope (SEM). Antimicrobial activities of synthesized NPs against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were determined by agar well diffusion technique.Results: The synthesized NPs varied in colour from dark brown to green and appears dominantly spherical, occasionally rod or triangular shaped with size ranging from 65-90 nm. UV spectroscopy absorption spectra of Ag, Zn and Cu NPs had absorbance peak at 267, 262 and 765 nm respectively. FT-IR spectrometry of Zn NP, Cu NP, and SMAE gave wave number ranging from 895.71-3320.67, 747.02-3225.45 and 658.25-3674.49 respectively. FT-IR analysis showed that SMAE acted as reducing and stabilizing agent while the NPs exhibited lower energy absorption band when compared to the plant extract. The NPs demonstrated higher antimicrobial activities against S. aureus than Ps. aeruginosa and E. coli. The antimicrobial activity was higher with copper NP than Ag and Zn NPs, and also higher than SMAE.Conclusion: The result from this study presents an indication for an alternative means for development of novel antimicrobial agents for clinical and biotechnological applications.Keywords: synthesis, nanoparticles, FT-IR, UV-visible spectrophotometry, antibacterial activitiesFrench Title: Synthèse verte de nanoparticules d'Ag, de Zn et de Cu à partir d'extrait aqueux de feuilles de Spondias mombin et évaluation de leur activité antibactérienneContexte: La nanotechnologie offre un avantage en tant que voie verte pour la synthèse de nanoparticules métalliques (NP) avec des extraits de plantes comme agent de coiffage. Spondias mombin est un arbre fruitier et ses extraits de feuilles possèdent des propriétés anxiolytiques,  hypoglycémiques, antiépileptiques, antipsychotiques, sédatives,  antioxydantes et antimicrobiennes. L’objectif de l’étude est de déterminer le potentiel antibactérien d’un simple produit non toxique de synthèse verte de nanoparticules métalliques (Ag, Zn et Cu) à l’aide de la feuille d’extraits aqueux de Spondias mombin (SMAE) en tant qu’agent réducteur et coiffant de la ions métalliques.Méthodologie: Les nanoparticules ont été caractérisées par analyse  spectrophotométrique UV visible, spectrophotomètre à transformée de Fourier infrarouge (FT-IR) et microscope électronique à balayage (MEB). Lesactivités antimicrobiennes de NP synthétisées contre Escherichia coli, Staphylococcus aureus et Pseudomonas aeruginosa ont été déterminées par une technique de diffusion sur puits d’agar.Résultats: Les NP synthétisés ont une couleur allant du brun foncé au vert et apparaissent principalement sphériques, parfois en bâtonnets ou en triangles, avec des tailles allant de 65 à 90 nm. Les spectres  d'absorption par spectroscopie UV des NP Ag, Zn et Cu présentaient des pics  d'absorbance à 267, 262 et 765 nm respectivement. La spectrométrie FT-IR de Zn NP, Cu NP et SMAE a donné un nombre d'onde allant de 895,71 à 3320,67, 747,02 à 3225,45 et 658,25 à 3674,49 respectivement. L'analyse FT-IR a montré que le SMAE agissait en tant qu'agent réducteur et  stabilisant, alors que les NP présentaient une bande d'absorption d'énergie inférieure à celle de l'extrait de plante. Les NP ont démontré des activités antimicrobiennes plus élevées contre S. aureus que Ps. aeruginosa et E. coli. L'activité antimicrobienne était plus élevée avec les NP en cuivre que dans les NP Ag et Zn, et également supérieure à celle du SMAE.Conclusion: le résultat de cette étude présente une indication d'un autre moyen de développement de nouveaux agents antimicrobiens pour des applications cliniques et biotechnologiques.Mots-clés: synthèse, nanoparticules, FT-IR, spectrophotométrie UV-visible, activités antibactériennes 

scholarly journals Antimicrobial effect of essential oils of some Fijian medicinal plant leaves on pathogenic bacteria

2016 ◽  
Vol 34 (2) ◽  
pp. 35
Author(s):  
Prayna P. P. Maharaj ◽  
Riteshma Devi ◽  
Surendra Prasad
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agent ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative

Fiji is highly populated with plants containing essential oils (EO). The essential oils extracted from the leaves of the selected Fijian leafy plants were screened against two Gram-negative bacteria (Salmonella typhimurium, Pseudomonas aeruginosa) and three Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis and Bacillus subtilis). The agar diffusion method was used to examine the antimicrobial activities of the extracted EO. All the EO tested showed antibacterial properties against one or more strains while none of the EO was active against Pseudomonas aeruginosa. Viburnum lantana (Wayfaring tree), Annona muricata (Soursop), Coleus amboinicus (Spanish thyme) and Cinnamomum zeylancium (Cinnamon) showed good inhibition against both Gram-positive and Gram-negative bacteria and proved as worthy source of antimicrobial agent. These findings will help the Pacific population to use the studied plants leaves as antimicrobial agent.

scholarly journals Antimicrobial activities of Saudi honey against Pseudomonas aeruginosa

2015 ◽  
Vol 22 (5) ◽  
pp. 521-525 ◽  
Author(s):  
Alaa A.M. Al-Nahari ◽  
Saad B. Almasaudi ◽  
El Sayed M. Abd El-Ghany ◽  
Elie Barbour ◽  
Soad K. Al Jaouni ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activities

scholarly journals WCK 5107 (Zidebactam) and WCK 5153 Are Novel Inhibitors of PBP2 Showing Potent “β-Lactam Enhancer” Activity against Pseudomonas aeruginosa, Including Multidrug-Resistant Metallo-β-Lactamase-Producing High-Risk Clones

2017 ◽  
Vol 61 (6) ◽  
Author(s):  
Bartolome Moya ◽  
Isabel M. Barcelo ◽  
Sachin Bhagwat ◽  
Mahesh Patel ◽  
German Bou ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
High Risk ◽  
Bactericidal Activity ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Enhancer Activity ◽  
Content Type ◽  
Steady State Kinetics ◽  
Time Kill ◽  
Derivatives Of

ABSTRACT Zidebactam and WCK 5153 are novel β-lactam enhancers that are bicyclo-acyl hydrazides (BCH), derivatives of the diazabicyclooctane (DBO) scaffold, targeted for the treatment of serious infections caused by highly drug-resistant Gram-negative pathogens. In this study, we determined the penicillin-binding protein (PBP) inhibition profiles and the antimicrobial activities of zidebactam and WCK 5153 against Pseudomonas aeruginosa, including multidrug-resistant (MDR) metallo-β-lactamase (MBL)-producing high-risk clones. MIC determinations and time-kill assays were conducted for zidebactam, WCK 5153, and antipseudomonal β-lactams using wild-type PAO1, MexAB-OprM-hyperproducing (mexR), porin-deficient (oprD), and AmpC-hyperproducing (dacB) derivatives of PAO1, and MBL-expressing clinical strains ST175 (bla VIM-2) and ST111 (bla VIM-1). Furthermore, steady-state kinetics was used to assess the inhibitory potential of these compounds against the purified VIM-2 MBL. Zidebactam and WCK 5153 showed specific PBP2 inhibition and did not inhibit VIM-2 (apparent Ki [Ki app] > 100 μM). MICs for zidebactam and WCK 5153 ranged from 2 to 32 μg/ml (amdinocillin MICs > 32 μg/ml). Time-kill assays revealed bactericidal activity of zidebactam and WCK 5153. LIVE-DEAD staining further supported the bactericidal activity of both compounds, showing spheroplast formation. Fixed concentrations (4 or 8 μg/ml) of zidebactam and WCK 5153 restored susceptibility to all of the tested β-lactams for each of the P. aeruginosa mutant strains. Likewise, antipseudomonal β-lactams (CLSI breakpoints), in combination with 4 or 8 μg/ml of zidebactam or WCK 5153, resulted in enhanced killing. Certain combinations determined full bacterial eradication, even with MDR MBL-producing high-risk clones. β-Lactam–WCK enhancer combinations represent a promising β-lactam “enhancer-based” approach to treat MDR P. aeruginosa infections, bypassing the need for MBL inhibition.

scholarly journals Bacteriological Study of Otitis Media and Its Antibiotic Susceptibility Pattern

2017 ◽  
Vol 15 (2) ◽  
pp. 124-129 ◽  
Author(s):  
Rabina Basnet ◽  
Sudarshan Sharma ◽  
Jid Chani Rana ◽  
Pradeep Kumar Shah
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Developing Countries ◽  
Otitis Media ◽  
Antibiotic Susceptibility ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Coagulase Negative Staphylococci ◽  
Bacteriological Study ◽  
Gram Negative

Background: Otitis Media is a prevailing and notorious infection in developing countries causing serious local damage and threating complication. Mainly in developing countries like Nepal, Otitis Media results because of illiteracy, poverty and poor hygiene. The aim of this study was to determine the profile of Otitis Media, its causative agents and their antibiotic susceptibility pattern.Methods: The study included 263 pus samples from 240 patients attending ENT department of Bharatpur hospital from May 2015 to January 2016. Samples were processed in microbiology department for bacteria using standard operating protocol. Antibiotic susceptibility testing was performed for all bacterial isolates by Kirby Bauer disc diffusion method and the results were interpreted according to clinical and laboratory standard institute (CLSI) guideline.Results: Out of 240 patients, 121 were female and 119 were male. Highest incidence of Otitis Media was observed in 1-10 year age group. Out of 263 samples taken from 240 patients, 216 showed bacterial growth. Gram negative bacteria predominated and the most common bacteria isolated were Staphylococcus aureus 36.11% followed by Pseudomonas aeruginosa 33.33% and Coagulase Negative Staphylococci 8.08%. All bacterial isolates were sensitive to gentamycin. Staphylococcus aureus was sensitive to Amikacin and gentamycin. All gram negative bacterial isolates were sensitive to Imipenem and gentamycin. 100% of Pseudomonas aeruginosa was sensitive to Imipenem.Conclusions: Staphylococcus aureus was the most predominant organism isolated from the pus swab followed by Pseudomonas aeruginosa and all the isolated organisms were sensitive to Gentamycin.

Antimicrobial Activity of New 2,4-Disubstituted Thiazolidinone Derivatives

2009 ◽  
Vol 64 (11-12) ◽  
pp. 785-789 ◽  
Author(s):  
Wael A. El-Sayed ◽  
Yasser K. Abdel-Monem ◽  
Nabil M. Yousif ◽  
Nashwa Tawfek ◽  
Mohamed T. Shaaban ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Antimicrobial Activities ◽  
Gram Positive ◽  
Streptomyces Species ◽  
Gram Negative

A number of new disubstituted 2,5-thiazolidinone derivatives were synthesized and tested for their antimicrobial activity against Bacillus subtilis (Gram-positive), Pseudomonas aeruginosa (Gram-negative), and Streptomyces species (Actinomycetes). They displayed different degrees of antimicrobial activities or inhibitory actions

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