Transgenic Expression of Therapeutic Proteins in Arabidopsis thaliana Seed

Author(s):  
Cory L. Nykiforuk ◽  
Joseph G. Boothe
Cell Research ◽  
2006 ◽  
Vol 16 (10) ◽  
pp. 830-840 ◽  
Author(s):  
Yan-Hong Chen ◽  
Xue-Min Wu ◽  
Hong-Qing Ling ◽  
Wei-Cai Yang

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173094 ◽  
Author(s):  
Alex Yi-Lin Tsai ◽  
Kin Chan ◽  
Chi-Yip Ho ◽  
Thomas Canam ◽  
Resmi Capron ◽  
...  

2011 ◽  
Vol 30 (8) ◽  
pp. 1493-1502 ◽  
Author(s):  
Peng Xianjun ◽  
Ma Xingyong ◽  
Fan Weihong ◽  
Su Man ◽  
Cheng Liqin ◽  
...  

Planta ◽  
2014 ◽  
Vol 240 (3) ◽  
pp. 575-583 ◽  
Author(s):  
Elzbieta Mietkiewska ◽  
Robin Miles ◽  
Aruna Wickramarathna ◽  
Ariff Firman Sahibollah ◽  
Michael S. Greer ◽  
...  

2008 ◽  
Vol 21 (6) ◽  
pp. 757-768 ◽  
Author(s):  
M. Hossein Borhan ◽  
Nick Gunn ◽  
Abigail Cooper ◽  
Sigrun Gulden ◽  
Mahmut Tör ◽  
...  

White blister rust in the Brassicaceae is emerging as a superb model for exploring how plant biodiversity has channeled speciation of biotrophic parasites. The causal agents of white rust across a wide breadth of cruciferous hosts currently are named as variants of a single oomycete species, Albugo candida. The most notable examples include a major group of physiological races that each are economically destructive in a different vegetable or oilseed crop of Brassica juncea (A. candida race 2), B. rapa (race 7), or B. oleracea (race 9); or parasitic on wild crucifers such as Capsella bursa-pastoris (race 4). Arabidopsis thaliana is innately immune to these races of A. candida under natural conditions; however, it commonly hosts its own molecularly distinct subspecies of A. candida (A. candida subsp. arabidopsis). In the laboratory, we have identified several accessions of Arabidopsis thaliana (e.g.,. Ws-3) that can permit varying degrees of rust development following inoculation with A. candida races 2, 4, and 7, whereas race 9 is universally incompatible in Arabidopsis thaliana and nonrusting resistance is the most prevalent outcome of interactions with the other races. Subtle variation in resistance phenotypes is evident, observed initially with an isolate of A. candida race 4, indicating additional genetic variation. Therefore, we used the race 4 isolate for map-based cloning of the first of many expected white rust resistance (WRR) genes. This gene was designated WRR4 and encodes a cytoplasmic toll-interleukin receptor-like nucleotide-binding leucine-rich repeat receptor-like protein that confers a dominant, broad-spectrum white rust resistance in the Arabidopsis thaliana accession Columbia to representative isolates of A. candida races 2, 4, 7, and 9, as verified by transgenic expression of the Columbia allele in Ws-3. The WRR4 protein requires functional expression of the lipase-like protein EDS1 but not the paralogous protein PAD4, and confers full immunity that masks an underlying nonhypersensitive incompatibility in Columbia to A. candida race 4. This residual incompatibility is independent of functional EDS1.


HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 614e-614
Author(s):  
Elizabeth A. Bihn ◽  
Robert J. Ferl

The 14-3-3 proteins were originally characterized in mammalian brains and were thought to be specifically involved in neurotransmitter production. Subsequent research has revealed that this family of proteins is ubiquitous in eucaryotic cells and is involved in a wide range of regulatory and signal transduction pathways. For instance, some 14-3-3 proteins have been associated with the signal transduction in response to fungal pathogen attack and to other environmental factors that affect transcription. In Arabidopsis, 10 isoforms of 14-3-3 have been isolated, raising the possibility that diversity of function may be governed by cellular and subcellular specificities of expression and localization. We have investigated the localization of certain 14-3-3 isoforms through transgenic expression of epitope-tagged 14-3-3s.


2018 ◽  
Vol 149 ◽  
pp. 1-11 ◽  
Author(s):  
María Eugenia Frey ◽  
Sebastián D'Ippolito ◽  
Alfonso Pepe ◽  
Gustavo Raúl Daleo ◽  
María Gabriela Guevara

Sign in / Sign up

Export Citation Format

Share Document