scholarly journals Transgenic expression of DwMYB2 impairs iron transport from root to shoot in Arabidopsis thaliana

Cell Research ◽  
2006 ◽  
Vol 16 (10) ◽  
pp. 830-840 ◽  
Author(s):  
Yan-Hong Chen ◽  
Xue-Min Wu ◽  
Hong-Qing Ling ◽  
Wei-Cai Yang
Keyword(s):  
Arabidopsis Thaliana ◽  
Iron Transport ◽  
Transgenic Expression

scholarly journals Transgenic expression of fungal accessory hemicellulases in Arabidopsis thaliana triggers transcriptional patterns related to biotic stress and defense response

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173094 ◽  
Author(s):  
Alex Yi-Lin Tsai ◽  
Kin Chan ◽  
Chi-Yip Ho ◽  
Thomas Canam ◽  
Resmi Capron ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Biotic Stress ◽  
Defense Response ◽  
Transgenic Expression

Improved drought and salt tolerance of Arabidopsis thaliana by transgenic expression of a novel DREB gene from Leymus chinensis

2011 ◽  
Vol 30 (8) ◽  
pp. 1493-1502 ◽  
Author(s):  
Peng Xianjun ◽  
Ma Xingyong ◽  
Fan Weihong ◽  
Su Man ◽  
Cheng Liqin ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Tolerance ◽  
Leymus Chinensis ◽  
Transgenic Expression ◽  
Drought And Salt Tolerance ◽  
Dreb Gene

scholarly journals Involvement of NRAMP1 from Arabidopsis thaliana in iron transport

2000 ◽  
Vol 347 (3) ◽  
pp. 749-755 ◽  
Author(s):  
Catherine CURIE ◽  
Jose M. ALONSO ◽  
Marie LE JEAN ◽  
Joseph R. ECKER ◽  
Jean-François BRIAT
Keyword(s):  
Arabidopsis Thaliana ◽  
Sequence Comparison ◽  
Mammalian Cells ◽  
Iron Transport ◽  
Iron Concentration ◽  
The Other ◽  
Yeast Mutant ◽  
High Affinity ◽  
Manganese Uptake ◽  
Do So

Nramp genes code for a widely distributed class of proteins involved in a variety of processes, ranging from the control of susceptibility to bacterial infection in mammalian cells and taste behaviour in Drosophila to manganese uptake in yeast. Some of the NRAMP proteins in mammals and in yeast are capable of transporting metal ions, including iron. In plants, iron transport was shown to require a reduction/Fe(II) transport system. In Arabidopsis thaliana this process involves the IRT1 and Fro2 genes. Here we report the sequence of five NRAMP proteins from A. thaliana. Sequence comparison suggests that there are two classes of NRAMP proteins in plants: A. thaliana (At) NRAMP1 and Oriza sativa (Os) NRAMP1 and 3 (two rice isologues) represent one class, and AtNRAMP2-5 and OsNRAMP2 the other. AtNramp1 and OsNramp1 are able to complement the fet3fet4 yeast mutant defective both in low- and high-affinity iron transports, whereas AtNramp2 and OsNramp2 fail to do so. In addition, AtNramp1 transcript, but not AtNramp2 transcript, accumulates in response to iron deficiency in roots but not in leaves. Finally, overexpression of AtNramp1 in transgenic A. thaliana plants leads to an increase in plant resistance to toxic iron concentration. Taken together, these results demonstrate that AtNramp1 participates in the control of iron homoeostasis in plants.

Combined transgenic expression of Punica granatum conjugase (FADX) and FAD2 desaturase in high linoleic acid Arabidopsis thaliana mutant leads to increased accumulation of punicic acid

Planta ◽  
2014 ◽  
Vol 240 (3) ◽  
pp. 575-583 ◽  
Author(s):  
Elzbieta Mietkiewska ◽  
Robin Miles ◽  
Aruna Wickramarathna ◽  
Ariff Firman Sahibollah ◽  
Michael S. Greer ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Linoleic Acid ◽  
Punica Granatum ◽  
Punicic Acid ◽  
High Linoleic Acid ◽  
Transgenic Expression

scholarly journals WRR4 Encodes a TIR-NB-LRR Protein That Confers Broad-Spectrum White Rust Resistance in Arabidopsis thaliana to Four Physiological Races of Albugo candida

2008 ◽  
Vol 21 (6) ◽  
pp. 757-768 ◽  
Author(s):  
M. Hossein Borhan ◽  
Nick Gunn ◽  
Abigail Cooper ◽  
Sigrun Gulden ◽  
Mahmut Tör ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Broad Spectrum ◽  
Rust Resistance ◽  
Functional Expression ◽  
Oilseed Crop ◽  
Transgenic Expression ◽  
Albugo Candida ◽  
Physiological Races ◽  
White Rust ◽  
Race 4

White blister rust in the Brassicaceae is emerging as a superb model for exploring how plant biodiversity has channeled speciation of biotrophic parasites. The causal agents of white rust across a wide breadth of cruciferous hosts currently are named as variants of a single oomycete species, Albugo candida. The most notable examples include a major group of physiological races that each are economically destructive in a different vegetable or oilseed crop of Brassica juncea (A. candida race 2), B. rapa (race 7), or B. oleracea (race 9); or parasitic on wild crucifers such as Capsella bursa-pastoris (race 4). Arabidopsis thaliana is innately immune to these races of A. candida under natural conditions; however, it commonly hosts its own molecularly distinct subspecies of A. candida (A. candida subsp. arabidopsis). In the laboratory, we have identified several accessions of Arabidopsis thaliana (e.g.,. Ws-3) that can permit varying degrees of rust development following inoculation with A. candida races 2, 4, and 7, whereas race 9 is universally incompatible in Arabidopsis thaliana and nonrusting resistance is the most prevalent outcome of interactions with the other races. Subtle variation in resistance phenotypes is evident, observed initially with an isolate of A. candida race 4, indicating additional genetic variation. Therefore, we used the race 4 isolate for map-based cloning of the first of many expected white rust resistance (WRR) genes. This gene was designated WRR4 and encodes a cytoplasmic toll-interleukin receptor-like nucleotide-binding leucine-rich repeat receptor-like protein that confers a dominant, broad-spectrum white rust resistance in the Arabidopsis thaliana accession Columbia to representative isolates of A. candida races 2, 4, 7, and 9, as verified by transgenic expression of the Columbia allele in Ws-3. The WRR4 protein requires functional expression of the lipase-like protein EDS1 but not the paralogous protein PAD4, and confers full immunity that masks an underlying nonhypersensitive incompatibility in Columbia to A. candida race 4. This residual incompatibility is independent of functional EDS1.

scholarly journals Cadmium Adsorption in Leaf Cell Walls Prevents Redistribution to Silique in Arabidopsis Thaliana Ecotypes Jm-1 and Kyo-0

2021 ◽  
Author(s):  
Yan Xiao ◽  
Dong Liu ◽  
Li Li ◽  
Zhenhua Zhang ◽  
Jin-Song Luo
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Walls ◽  
Iron Transport ◽  
Agricultural Practices ◽  
Pectin Methylesterase ◽  
Future Research ◽  
Tetraacetic Acid ◽  
Leaf Cell ◽  
Rapid Progress ◽  
Soil Cd

Abstract Background: Along with the rapid progress of industrialization and urbanization in the world, soil Cd pollution has become an increasingly serious problem. Phytoremediation has been widely used to mitigate heavy metal pollution in soils; however, it is difficult to reduce the Cd content in the grains of food crops using Cd pollution remediation techniques. Results: Here, we found that the Cd concentrations in the leaves, stems, and siliques of Arabidopsis thaliana (A. thaliana) ecotype Jm-1 were higher than in the ecotype Kyo-0. The Cd concentrations in the cell walls (CW) of the leaves were lower in Jm-1 than in Kyo-0, while the concentrations in the CW of the stem and silique were significantly higher in Jm-1 than in Kyo-0. The Cyclohexane Diamine Tetraacetic Acid (CDTA)-pectin and hemicellulose in Kyo-0 had higher Cd concentrations than those of Jm-1. The pectin methylesterase (PME) activity was higher in Kyo-0 than in Jm-1, and the expression levels of PME1, PME2, PME12, and PME25 were upregulated in Kyo-0 after Cd treatment. In addition, no significant differences in the Cd concentrations were found in the xylem of the two ecotypes, while the Cd concentration in the phloem was significantly higher in Jm-1 than in Kyo-0. The expression of iron transport-related genes showed that only YSL3 and ZIP11 had significant differences between the two ecotypes after Cd treatment, and the expression of the vacuolar Cd compartment-related genes that are responsible for transferring Cd from the cytoplasm to the vacuole showed that only CAX1 expression was significantly higher in Kyo-0 than in Jm-1. Conclusions: Kyo-0 accumulated less Cd than Jm-1 in the silique, which may be because (1) the activity of PME that is mainly regulated by PME1, PME2, PME12, and PME25 was higher in Kyo-0 leaves, leading to more Cd chelation in the pectin of the CWs, and (2) the expression of YSL3 was induced to regulate the transport of Cd in the phloem, thus reducing the transport of Cd to the silique. This study would benefit future research and agricultural practices.

scholarly journals Subcellular Localization of 14-3-3 Regulatory Proteins in Arabidopsis thaliana

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 614e-614
Author(s):  
Elizabeth A. Bihn ◽  
Robert J. Ferl
Keyword(s):  
Signal Transduction ◽  
Arabidopsis Thaliana ◽  
Environmental Factors ◽  
Subcellular Localization ◽  
Fungal Pathogen ◽  
Regulatory Proteins ◽  
Signal Transduction Pathways ◽  
Transgenic Expression ◽  
Subsequent Research ◽  
Wide Range

The 14-3-3 proteins were originally characterized in mammalian brains and were thought to be specifically involved in neurotransmitter production. Subsequent research has revealed that this family of proteins is ubiquitous in eucaryotic cells and is involved in a wide range of regulatory and signal transduction pathways. For instance, some 14-3-3 proteins have been associated with the signal transduction in response to fungal pathogen attack and to other environmental factors that affect transcription. In Arabidopsis, 10 isoforms of 14-3-3 have been isolated, raising the possibility that diversity of function may be governed by cellular and subcellular specificities of expression and localization. We have investigated the localization of certain 14-3-3 isoforms through transgenic expression of epitope-tagged 14-3-3s.

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