QTL Analysis and Map-Based Cloning of Salt Tolerance Gene in Rice

2012 ◽  
pp. 69-82 ◽  
Author(s):  
Ji-Ping Gao ◽  
Hong-Xuan Lin
Keyword(s):  
Salt Tolerance ◽  
Qtl Analysis ◽  
Tolerance Gene ◽  
Salt Tolerance Gene

scholarly journals Identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Xinpeng Qi ◽  
Man-Wah Li ◽  
Min Xie ◽  
Xin Liu ◽  
Meng Ni ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Wild Soybean ◽  
Whole Genome ◽  
Tolerance Gene ◽  
Salt Tolerance Gene

scholarly journals Enhanced salt tolerance of transgenic tobacco expressing a wheat salt tolerance gene

2016 ◽  
Vol 40 ◽  
pp. 727-735 ◽  
Author(s):  
Musa KAVAS ◽  
Mehmet Cengiz BALOĞLU ◽  
Ayşe Meral YÜCEL ◽  
Hüseyin Avni ÖKTEM
Keyword(s):  
Salt Tolerance ◽  
Transgenic Tobacco ◽  
Tolerance Gene ◽  
Salt Tolerance Gene

scholarly journals In Silico Design of PCR Primers to Amplify the Salt Tolerance Gene in Soybean

2018 ◽  
Vol 6 (6) ◽  
Author(s):  
Florentina Kusmiyati ◽  
Sutarno Sutarno ◽  
Bagus Herwibawa
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
In Silico ◽  
Pcr Primers ◽  
Significant Similarity ◽  
Web Based ◽  
Conserved Region ◽  
Tolerance Gene ◽  
Salt Tolerance Gene ◽  
Primer Sequence

Soybean yield is decreased because of many stresses such as salt stress. Ionic and osmotic stresses are the effects of salt stress. An effective way of maintaining sustainable production in salt-affected soil is through breeding high salt tolerance soybean, which can be detected by PCR. The optimal PCR plays an important role in gene expression analysis. The success of a PCR-based method largely depends on the optimal primer sequence analysis in silico prior to a wet-bench experiment. Here we described designing of primer using web-based tools. Many types of online primer design software are available, which can be used free of charge to design desirable primers. The objective was to design of PCR primers to amplify the salt tolerance gene in soybean. A highly conserved region of 411 bases was detected by Clustal Omega. Primers were predicted using Primer3 based on conserved region, considering ideal conditions for primer length, hairpin, dimer, Tm, and GC%. The predicted forward and reverse primers were validated using NetPrimer. Both forward and reverse primers have shown significant similarity with salt tolerance gene and recommended to be used to amplify the salt tolerance gene in soybean.

scholarly journals The optimization of gene transfer to tomato and the study of expression possibility of salt-tolerance gene (SOS3)

2020 ◽  
Vol 7 (1) ◽  
pp. 76-84
Author(s):  
Elham Mohajel Kazemi ◽  
Maghsoud Pazhohandeh ◽  
Parisa Jonoubi ◽  
Mina Kazemian ◽  
◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Gene Transfer ◽  
Tolerance Gene ◽  
Salt Tolerance Gene

scholarly journals Improvement of water use efficiency in rice by expression of HARDY, an Arabidopsis drought and salt tolerance gene

2007 ◽  
Vol 104 (39) ◽  
pp. 15270-15275 ◽  
Author(s):  
A. Karaba ◽  
S. Dixit ◽  
R. Greco ◽  
A. Aharoni ◽  
K. R. Trijatmiko ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Water Use Efficiency ◽  
Water Use ◽  
Drought And Salt Tolerance ◽  
Use Efficiency ◽  
Tolerance Gene ◽  
Salt Tolerance Gene

Assessment of salt tolerance and analysis of the salt tolerance gene Ncl in Indonesian soybean germplasm

2019 ◽  
Vol 17 (03) ◽  
pp. 265-271
Author(s):  
D. Cao ◽  
Y. L. Yan ◽  
D. H. Xu
Keyword(s):  
Salt Tolerance ◽  
Dry Weight ◽  
Salt Tolerant ◽  
Soil Medium ◽  
Spad Value ◽  
Shoot Dry Weight ◽  
Leaf Chlorophyll ◽  
Tolerance Gene ◽  
Salt Tolerance Gene ◽  
Hydroponic Conditions

AbstractSoybean [Glycine max (L.) Merr.] is one of the most important legume crops in the world. However, soybean grain yield is extensively affected by environmental stresses such as soil salinity. In this study, we evaluated the germplasm of 51 Indonesian soybean accessions for salt tolerance to identify salt-tolerant germplasms for possible use in breeding for soybean salt tolerance. Based on experiments under hydroponic conditions, adding 100 mM of NaCl to a 1/2 concentration of Hoagland and Arnon solution, several Indonesian soybean germplasms, such as Java 7, Seputih Raman, Tambora, Ringgit (JP 30217), Sinyonya (early) and Sinyonya (late) were identified as salt-tolerant in terms of salt tolerance rate (STR) and leaf chlorophyll content (SPAD value) taken with the Konica Minolta SPAD-502 chlorophyll meter. The selected salt-tolerant germplasms were further evaluated under soil medium cultivation in pots irrigated with 100 mM NaCl for around 5 weeks. The six selected soybean germplasms again showed higher salt tolerance in terms of SPAD, STR and shoot dry weight. Expression analysis of the salt tolerance gene Ncl revealed a significant positive correlation between Ncl expression and salt tolerance, suggesting that Ncl is essential for salt tolerance in the Indonesian soybean germplasms we tested. The salt-tolerant Indonesian soybean germplasms identified in this study could be used in local soybean breeding practices for the improvement of salt tolerance.

scholarly journals Rice breeding on salt tolerance

2019 ◽  
pp. 22-27
Author(s):  
P. I. Kostylev ◽  
E. B. Kudashkina ◽  
E. V. Krasnova ◽  
N. N. Vozhzhova
Keyword(s):  
Salt Tolerance ◽  
Agricultural Production ◽  
Distilled Water ◽  
Rice Breeding ◽  
Standard Variety ◽  
Nacl Solution ◽  
Salt Tolerant ◽  
Homozygous State ◽  
Tolerance Gene ◽  
Salt Tolerance Gene

Salt tolerance of rice is of great importance in agricultural production, since productivity of grain grown on saline lands largely depends on it. The paper deals with determining the presence of salt tolerance gene “Saltol” in rice hybrids obtained from crossing contrasting samples. To analyze salt tolerance, there were used seeds germinated in cups with distilled water and 1.5% NaCl solution, after which 10-day-old seedlings were weighed and their ratio to the control was determined. As a result of studies, there have been identified salt tolerant lines carrying the gene “Saltol” in a homozygous state. In the control nursery there were studied the best samples on productivity and elements of its structure. Two lines “7328” and “7322” were selected from the hybrid IR 52713-2B-8-2B-1-2 x Novator which significantly exceeded the standard variety “Yuzhanin” on 0.57-1.28 t/ha. On average for 2 years, they produced 6.82-7.53 t/ha (6.25 t/ha of the standard variety).

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