The Role of Ethylene and Other Signals in the Regulation of Fe Deficiency Responses by Dicot Plants

2016 ◽  
pp. 277-300 ◽  
Author(s):  
Francisco Javier Romera ◽  
Carlos Lucena ◽  
María José García ◽  
Esteban Alcántara ◽  
Rafael Pérez-Vicente
Keyword(s):  
Fe Deficiency

scholarly journals Physiological and interactomic analysis reveals versatile functions of Arabidopsis 14-3-3 quadruple mutants in response to Fe deficiency

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jing Gao ◽  
Paula J. M. van Kleeff ◽  
Ka Wan Li ◽  
Albertus H. de Boer
Keyword(s):  
Iron Acquisition ◽  
Tca Cycle ◽  
Utilization Efficiency ◽  
Fe Deficiency ◽  
Transcriptional Level ◽  
Functional Aspects ◽  
Clear Explanation ◽  
Physiological Analysis ◽  
Proper Operation

AbstractTo date, few phenotypes have been described for Arabidopsis 14-3-3 mutants or the phenotypes showing the role of 14-3-3 in plant responding to abiotic stress. Although one member of the 14-3-3 protein family (14-3-3 omicron) was shown to be involved in the proper operation of Fe acquisition mechanisms at physiological and gene expression levels in Arabidopsis thaliana, it remains to be explored whether other members play a role in regulating iron acquisition. To more directly and effectively observe whether members of 14-3-3 non-epsilon group have a function in Fe-deficiency adaptation, three higher order quadruple KOs, kappa/lambda/phi/chi (klpc), kappa/lambda/upsilon/nu(klun), and upsilon/nu/phi/chi (unpc) were generated and studied for physiological analysis in this study. The analysis of iron-utilization efficiency, root phenotyping, and transcriptional level of Fe-responsive genes suggested that the mutant with kl background showed different phenotypes from Wt when plants suffered Fe starved, while these phenotypes were absent in the unpc mutant. Moreover, the absence of the four 14-3-3 isoforms in the klun mutant has a clear impact on the 14-3-3 interactome upon Fe deficiency. Dynamics of 14-3-3-client interactions analysis showed that 27 and 17 proteins differentially interacted with 14-3-3 in Wt and klun roots caused by Fe deficiency, respectively. Many of these Fe responsive proteins have a role in glycolysis, oxidative phosphorylation and TCA cycle, the FoF1-synthase and in the cysteine/methionine synthesis. A clear explanation for the observed phenotypes awaits a more detailed analysis of the functional aspects of 14-3-3 binding to the target proteins identified in this study.

Role of sulphur conferring differential tolerance to iron deficiency in Pisum sativum

Biologia ◽  
2015 ◽  
Vol 70 (7) ◽  
Author(s):  
Ahmad H. Kabir ◽  
Nicholas G. Paltridge ◽  
James Stangoulis
Keyword(s):  
Foliar Application ◽  
Reductase Activity ◽  
Foliar Spray ◽  
Fe Deficiency ◽  
Proton Extrusion ◽  
Severe Stunting ◽  
Leaf Chlorophyll ◽  
S Deficiency ◽  
Growth Features

AbstractThis study investigated the effects of sulphur foliar spray and S deprivation on Fe deficiency responses in two contrasting pea genotypes, Santi (tolerant) and Parafield (sensitive). Foliar application of sulphur enhanced morphological growth features, leaf chlorophyll score and root Fe chelate reductase activity predominantly in Santi and to a lesser extent in Parafield. These capacities eventually contribute to the higher Fe deficiency tolerance in Santi. These results are also important in terms of ameliorating Fe deficiency effects in peas through S foliar spray. Further, targeted investigation was performed on S deprivation in Santi and Parafield. S deprivation caused severe stunting, chlorosis and wrinkling in leaves and caused decrease in leaf Fe concentrations both in Santi and Parafield under Fe deficiency. S deprivation also led to a significant decrease in Fe chelate reductase and proton extrusion activities in both genotypes in Fe shortage. We conclude that S deficiency exacerbates Fe deficiency in peas by preventing the induction of the Fe chelate reductase activity and proton extrusion in roots. Taken together, these data confirm that Fe deficiency symptom expression and the Fe deficiency responses in peas are largely determined by S nutritional status.

scholarly journals Glutathione regulates subcellular iron homeostasis via transcriptional activation of iron responsive genes in Arabidopsis

2021 ◽  
Author(s):  
Ranjana Shee ◽  
Soumi Ghosh ◽  
Pinki Khan ◽  
Salman Sahid ◽  
Chandan Roy ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Transcriptional Activation ◽  
Iron Homeostasis ◽  
Fe Deficiency ◽  
Fe Content ◽  
Increased Sensitivity ◽  
Fe Homeostasis ◽  
Bhlh Transcription Factors ◽  
Dependent Pathway

Glutathione (GSH) is a ubiquitous molecule known to regulate various physiological and developmental phenomena in plants. Recently, its involvement in regulating iron (Fe) deficiency response was established in Arabidopsis. However, the role of GSH in modulating subcellular Fe homeostasis remained elusive. In this study, we dissected the role of GSH in regulating Fe homeostasis in Arabidopsis shoots under Fe limited conditions. The two GSH depleted mutants, cad2-1 and pad2-1 displayed increased sensitivity to Fe deficiency with smaller rosette diameter and higher chlorosis level compared with the Col-0 plants. Interestingly, the expression of the vacuolar Fe exporters, AtNRAMP3 and AtNRAMP4, chloroplast Fe importer, AtPIC1, along with AtFer1 and AtIRT1 were significantly down-regulated in these mutants. The expression of these genes were up-regulated in response to exogenous GSH treatment while treatment with BSO, a GSH inhibitor, down-regulated their expression. Moreover, the mutants accumulated higher Fe content in the vacuole and lower in the chloroplast compared with Col-0 under Fe limited condition suggesting a role of GSH in modulating subcellular Fe homeostasis. This regulation was, further, found to involve a GSNO-dependent pathway. Promoter analysis revealed that GSH induced the transcription of these genes presumably via S-nitrosylation of different Fe responsive bHLH transcription factors.

Flour fortification with iron has no impact on anaemia in urban Brazilian children

2012 ◽  
Vol 15 (10) ◽  
pp. 1796-1801 ◽  
Author(s):  
Maria CF Assunção ◽  
Iná S Santos ◽  
Aluísio JD Barros ◽  
Denise P Gigante ◽  
Cesar G Victora
Keyword(s):  
Venous Blood ◽  
Transferrin Saturation ◽  
Nationwide Survey ◽  
Population Based ◽  
Poor Quality ◽  
Fe Deficiency ◽  
Local Study ◽  
The Impact ◽  
Brazilian Children

AbstractObjectiveTo verify the impact of flour fortification on anaemia in Brazilian children. The survey also investigated the role of Fe deficiency as a cause of anaemia and estimated the bioavailability of the Fe in the children's diet. This local study was complemented by a nationwide survey of the types of Fe compounds added to flour.DesignSeries of population-based surveys conducted in 2004 (baseline study), 2005, 2006 and 2008.SettingPelotas, Rio Grande do Sul, Brazil.SubjectsChildren under 6 years of age residing in the urban area of the city of Pelotas, Southern Brazil (n 507 in 2004; n 960 in 2005; n 893 in 2006; n 799 in 2008). In 2008, a sub-sample of children (n 114) provided venous blood samples to measure body Fe reserve parameters (ferritin and transferrin saturation).ResultsWe found no impact of fortification, with an increase in anaemia prevalence among children under 24 months of age. Hb levels decreased by 0·9 g/dl in this age group between 2004 and 2008 (10·9 g/dl to 10·0 g/dl; P < 0·001). Roughly 50 % of cases of anaemia were estimated to be due to Fe deficiency. Half of the mills surveyed used reduced Fe to fortify wheat flour. Total Fe intake from all foodstuffs was adequate for 88·6 % of the children, but its bioavailability was only 5 %.ConclusionsThe low bioavailability of the Fe compounds added to flours, combined with the poor quality of children's diets, account for the lack of impact of mandatory fortification.

scholarly journals The influence of vitamin A status on iron-deficiency anaemia in anaemic adolescent schoolgirls in Myanmar

2013 ◽  
Vol 17 (10) ◽  
pp. 2325-2332 ◽  
Author(s):  
Min Kyaw Htet ◽  
Umi Fahmida ◽  
Drupadi Dillon ◽  
Arwin Akib ◽  
Budi Utomo ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Vitamin A ◽  
Iron Deficiency Anaemia ◽  
Fe Deficiency ◽  
Subclinical Inflammation ◽  
Serum Retinol ◽  
Deficiency Anaemia ◽  
Vitamin A Status ◽  
High Prevalence

AbstractObjectiveThe present study was conducted to investigate reasons for the high prevalence of anaemia among adolescent schoolgirls and to elucidate the role of vitamin A in contributing to Fe-deficiency anaemia (IDA).DesignAmong 1269 schoolgirls who were previously screened for anaemia (Hb < 120 g/l), 391 anaemic girls were further assessed for Fe, vitamin A and subclinical inflammation status. Fe and vitamin A indicators were corrected for inflammation and were compared in the Fe-deficient and non-deficient groups as well as between those with and without inflammation. Logistic regression was done to determine whether vitamin A status and subclinical inflammation were risk factors for Fe deficiency. The differences in Fe status among tertiles of vitamin A concentrations were assessed using ANOVA.SettingMyanmar.SubjectsAdolescent schoolgirls (n 391).ResultsOne-third of the anaemia (30·4 %) was IDA. Prevalence of low vitamin A status (serum retinol <1·05 μmol/l) was 31·5 %. Fe and vitamin A status were significantly different between the IDA and non-IDA groups and also based on their inflammation status. Logistic regression showed that low vitamin A status was a significant predictor for being Fe deficient (OR = 1·81; 95 % CI 1·03, 3·19 and OR = 2·31; 1·31, 4·07 in the middle (1·056–1·298 μmol/l) and low (≤1·056 μmol/l) vitamin A tertiles, respectively). ANOVA showed that better Fe status was associated with a higher concentration of serum retinol but only in IDA.ConclusionsFe deficiency was not the main cause of anaemia in the present population. The role of vitamin A as well as other micronutrients should be taken into account in addressing the problem of anaemia.

scholarly journals Transcriptomic Profiling of Fe-Responsive lncRNAs and Their Regulatory Mechanism in Rice

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 567
Author(s):  
Shoudong Wang ◽  
Shuo Sun ◽  
Runze Guo ◽  
Wenying Liao ◽  
Huixia Shou
Keyword(s):  
Regulatory Mechanism ◽  
Transcriptional Response ◽  
Fe Deficiency ◽  
Rna Seq ◽  
Systematic Screening ◽  
Yield And Quality ◽  
Non Coding Rnas ◽  
Fe Homeostasis ◽  
Insight Into

Iron (Fe) deficiency directly affects crop growth and development, ultimately resulting in reduced crop yield and quality. Recently, long non-coding RNAs (lncRNAs) have been demonstrated to play critical regulatory roles in a multitude of pathways across numerous species. However, systematic screening of lncRNAs responding to Fe deficiency and their regulatory mechanism in plants has not been reported. In this work, 171 differently expressed lncRNAs (DE-lncRNAs) were identified based on analysis of strand-specific RNA-seq data from rice shoots and roots under Fe-deficient conditions. We also found several lncRNAs, which could generate miRNAs or act as endogenous target mimics to regulate expression of Fe-related genes. Analysis of interaction networks and gene ontology enrichment revealed that a number of DE-lncRNAs were associated with iron transport and photosynthesis, indicating a possible role of lncRNAs in regulation of Fe homeostasis. Moreover, we identified 76 potential lncRNA targets of OsbHLH156, a key regulator for transcriptional response to Fe deficiency. This study provides insight into the potential functions and regulatory mechanism of Fe-responsive lncRNAs and would be an initial and reference for any further studies regarding lncRNAs involved in Fe deficiency in plants.

scholarly journals Levels of serum transferrin receptor and its response to Fe-supplement in Fe-deficient children

2006 ◽  
Vol 96 (6) ◽  
pp. 1134-1139 ◽  
Author(s):  
Xiao-Ming Lin ◽  
Cheng-Ye Ji ◽  
Wen-Jing Liu ◽  
Zhu Long ◽  
Xiao-Yi Shen
Keyword(s):  
Transferrin Receptor ◽  
Operating Characteristic ◽  
Characteristic Curve ◽  
Fe Deficiency ◽  
Serum Transferrin ◽  
Diagnostic Efficacy ◽  
Republic Of China ◽  
Serum Transferrin Receptor ◽  
Before And After

The object of the present study was to investigate the levels of serum transferrin receptor (sTfR) and its response to Fe supplementation in Fe-deficient children and the role of sTfR in detecting Fe deficiency and assessing the efficacy of Fe supplementation. According to the diagnostic standard, 1006 children, aged 6–14 years in Fangshan district, Beijing, Peoples Republic of China, were divided into four groups: normal; Fe store depletion (IDs); Fe deficiency erythropoiesis (IDE); Fe deficiency anaemia (IDA). sTfR was determined and transferrin receptor-ferritin (TfR-F) index was calculated in 238 children, sixty-four normal and 174 Fe deficient. Children were administered a NaFeEDTA capsule containing 60 mg Fe once per week for the IDs and IDE groups and three times per week for the IDA group for nine consecutive weeks. The parameters reflecting Fe status and sTfR were determined before and after Fe supplementation. The levels of sTfR and TfR-F index in Fe-deficient children were significantly higher than those in the normal group. The receiver operating characteristic curve showed that sTfR has proper diagnostic efficacy for functional Fe deficiency. After Fe supplementation, the level of sTfR was significantly decreased in children with IDs, but not in children with IDE and IDA, while TfR-F index was significantly decreased in Fe-deficient children. sTfR is a reliable indicator for detecting functional Fe deficiency, and TfR-F index is a sensitive parameter for assessing the efficacy of Fe supplementation.

The role of Fe deficiency in FeySe0.5Te0.5 samples prepared by a melting process

2013 ◽  
Vol 494 ◽  
pp. 69-73 ◽  
Author(s):  
A. Sala ◽  
A. Palenzona ◽  
C. Bernini ◽  
F. Caglieris ◽  
M.R. Cimberle ◽  
...  
Keyword(s):  
Melting Process ◽  
Fe Deficiency

scholarly journals Iron acquisition across outer membrane: The role of major outer membrane protein Slr1908 under iron supplemented conditions in Synechocystis 6803

2021 ◽  
Author(s):  
Rachna Agarwal ◽  
Sharmistha Dutta Choudhury ◽  
Akipeddi V S S Narayana Rao
Keyword(s):  
Outer Membrane ◽  
Insertional Mutagenesis ◽  
Iron Acquisition ◽  
Fe Deficiency ◽  
Active Iron ◽  
Radiotracer Uptake ◽  
Mn Content ◽  
High Oxidative Stress ◽  
Fe Acquisition

AbstractThe Ton-B dependent outer membrane (OM) transporters are responsible for active iron (Fe) import in Synechocystis sp. strain PCC 6803 (S. 6803 or WT) under Fe depletion. However, the mechanism of Fe acquisition under Fe supplemented conditions remains uncharacterised. In the present study, functional role of OMP Slr1908 in S. 6803 was addressed by insertional mutagenesis. The Δslr1908 cells exhibited slower growth in the first week in comparison to the WT and displayed an absorption and 77K fluorescence spectrum typical of Fe deficiency. Indeed, the mutant had ∼ 80% less Fe as confirmed by atomic absorption spectroscopy and 55Fe-radiotracer uptake. The iron deficiency was paralleled with low Mn content. The mutant had low SOD content as well as activity, less cytochromes, less chlorophyll content, less Fv/ Fm, lower ETRII and high oxidative stress in comparison to the WT at the end of first week. Interestingly, the mutant showed transcriptional upregulation of iron stress induced protein isiA and isiB signifying intracellular Fe deficiency. Upregulation of OMP Slr0042 was also observed at RNA and protein level. The results indicate that Slr1908 is a major Fe uptake OMP in S. 6803 the deletion of which leads to initial slow growth that gets partially offset by induction of other Fe importing OMPs.

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