Characteristics of Alcaligenes sp. LS2T Heterotrophic and Aerobic Ammonium Removal for Potential Livestock’s Wastewater Treatment

Author(s):  
Aldyon Restu Azkarahman ◽  
Yuny Erwanto ◽  
Widodo Hadisaputro ◽  
Lies Mira Yusiati ◽  
Nanung Agus Fitriyanto
2012 ◽  
Vol 65 (11) ◽  
pp. 2084-2090 ◽  
Author(s):  
YuXiang Liu ◽  
YaQing Li ◽  
YongKang Lv

This study investigated some factors affecting ammonium removal and nitrite accumulation by Alcaligenes faecalis C16, which was isolated from the activated sludge of a coking wastewater treatment plant. Nitrite was produced from ammonium only in the presence of citrate, acetate, meat extract, peptone or ethanol. The highest amount of nitrite was found with citrate as carbon source. A. faecalis C16 could not use glucose, fructose, sucrose and methanol. Under the optimum conditions of initial pH 6.0, C/N 14, 30 °C and 120 rpm, a maximum nitrite accumulation of 28.29 mg/L NO2−-N was achieved when the organism grew with citrate in four days. Nitrite accumulation increased with the increase of NH4+-N. Furthermore, A. faecalis C16 was shown to have phenol-degrading capacity during ammonium removal. Metabolism of phenol resulted in acidification of the media, which is not favorable for nitrification, whereas many other carbon sources made the medium more alkaline. However, no inhibitory effect by phenol was observed when phenol and acetate were used as mixed carbon source at different phenol/sodium acetate (P/S) ratios and their pH values were all controlled above 9.2 or P/S ratios below 5:5. These results suggested that A. faecalis C16 has some potential application in industrial wastewater treatment systems.


2011 ◽  
Vol 108 ◽  
pp. 183-188 ◽  
Author(s):  
Yan Li Lǚ ◽  
Yan Qiu Wang ◽  
Ming Jun Shan ◽  
Da Wei Pan ◽  
Tie Feng Li ◽  
...  

The paper discusses the change regulation, characteristics and designation function of the aerobic biological phase in the biological treatment system of single reactor system for high ammonium removal over nitrite-anaerobic ammonium oxidation (SHARON-ANAMMOX) of Coal Tar Processing wastewater treatment. And soma advice is given for the adjustment and supervision of the similar biological disposing system of industrial dirty water.


2021 ◽  
Vol 13 (2) ◽  
pp. 956
Author(s):  
Giovanna Salbitani ◽  
Simona Carfagna

In plant cells, ammonium is considered the most convenient nitrogen source for cell metabolism. However, despite ammonium being the preferred N form for microalgae, at higher concentrations, it can be toxic, and can cause growth inhibition. Microalgae’s tolerance to ammonium depends on the species, with various taxa showing different thresholds of tolerability and symptoms of toxicity. In the environment, ammonium at high concentrations represents a dangerous pollutant. It can affect water quality, causing numerous environmental problems, including eutrophication of downstream waters. For this reason, it is important to treat wastewater and remove nutrients before discharging it into rivers, lakes, or seas. A valid and sustainable alternative to conventional treatments could be provided by microalgae, coupling the nutrient removal from wastewater with the production of valuable biomass. This review is focused on ammonium and its importance in algal nutrition, but also on its problematic presence in aquatic systems such as wastewaters. The aim of this work is to provide recent information on the exploitation of microalgae in ammonium removal and the role of ammonium in microalgae metabolism.


2019 ◽  
Author(s):  
Judit Canellas ◽  
Ana Soares ◽  
Bruce Jefferson

One of the biggest challenges to implementing ion exchange processes in full scale at wastewater treatment works is regeneration of the media. In this paper, regeneration efficiency as a function of brine type, brine reuse, molarity and pH was investigated for the zeolite MesoLite treating synthetic solutions of ammonium. When pretreating the zeolite with KCl rather than NaCl, a 30% improvement in regeneration efficiency was found in the first cycle, which dropped to a 10% improvement in the fifth cycle. For both systems, the observed uptake capacity during the load cycle remained constant, indicating that both were effective and that the brine could be reused five times without deterioration in the performance of the zeolite. The use of KCl was more effective at lower molarities than NaCl such that equivalent regeneration efficiencies were observed at 1.0M and 0.1M for NaCl and KCl respectively. Alteration of the pH between 9 and 12 had no impact on the regeneration efficiency. However, operation at pH 12 was possible without brine. Taken together, these findings indicate that the choice of regenerant can have significant implications on regeneration efficiency and that potassium chloride might be a potentially viable alternative choice.


2018 ◽  
Vol 73 ◽  
pp. 05005
Author(s):  
Syakira Hana ◽  
Novelendah Lidiya ◽  
Pertiwi Kartika ◽  
Syahidah Rusyda ◽  
Purwono ◽  
...  

Ammonium, a form of nitrogen, causes eutrophication and also can be toxic for fish. The existence of ammonium in surface water arises from human and animal waste, as well as from organic materials oxidation derived from industrial or residential wastewater. Consequently, wastewater with high ammonium content must be processed properly prior to disposal. One of the commonly used treatments for wastewater is attached biological wastewater treatment, which can be applied with a fixed bed reactor. Fixed bed reactor was being inoculated with ecotru bacteria which were fed with artificial wastewater containing ammonium of 50 mg NH4+-N/L. In this study, it was found that the microorganisms contained in ecotru are Geobacillus toebii bacteria. The ammonium removal efficiency was 99,63% and the way Geobacillus toebii contribute on ammonium removal was experimentally studied in this study.


2020 ◽  
Author(s):  
◽  
Yrielle Roets ◽  

South Africa’s freshwater resources, including rivers, man-made lakes and groundwater are under severe threat due to an ever-expanding population and economy, which is depleting these resources. The increase in population has a direct correlation with the increase in wastewater generated. The remaining fresh water resources need to be preserved therefore recycling of wastewater, to replenish our water supplies and preserve the environment, is a solution to the problem. For a developing country, it is important to use treatment methods that are cost effective and do not exert a negative impact on the environment, such as biological wastewater treatment options. One of the systems commonly used in biological wastewater treatment is the fluidized-bed bioreactor (FBBR) due to its advantages such as higher biomass concentration and a higher mass transfer thus resulting in a higher rate of biodegradation. This study focused on evaluating the efficacy of augmenting with Bacillus spp. to enhance the bioremediation of wastewater using a FBBR. Bacillus spp. used in this study were isolated from a municipal wastewater treatment plant (10 isolates) and the remaining three isolates were selected from the CSIR Bacillus database. The isolates (13 in total) were screened for 1) their ability grow in wastewater, 2) ability to reduce high concentrations of COD, ammonium, nitrates and phosphates in flask studies containing synthetic wastewater (SWW) and 3) ability to produce common enzymes such as amylase, cellulase, lipase and protease. Isolates showed varying bioremediation potential for different compounds analysed. Isolate B006 showed the highest phosphate removal rate (3.290 mg.L-1.h-1) where as D005 showed the highest growth rate (0.955 h-1), COD reduction rate (55 mg.L-1.h-1) and cellulase activity (5.485 mm) among all the isolates. Isolate D014 presented the highest ammonium removal rate (12.43 mg.L-1.h-1), amylase (5.00 mm) and protease (10.00 mm) activity whilst B001 displayed the highest nitrate removal rate (9.4 mg.L-1.h-1). The results for the individual assays were assessed and weighted in a matrix and the isolates that scored above 50% were selected for consortium studies. Four Bacillus spp. that scored above 50% in the scoring matrix were then evaluated for their ability to co-exist as a consortium. The consortium studies were then compared with results obtained for individual isolates. The selected Bacillus isolates were identified and assessed for their safety to the environment and to the end user. Identification was conducted using 16s rDNA sequencing and results showed that B006 identified as B. cereus, D005 as B. cereus and D014 as B. subtilis. Isolates, B006 and D005 were further assessed for enterotoxin production and the presence of anthrax virulent plasmids pX01 and pX02. After conducting the biosafety assays, the isolates were rendered safe for use. The isolates were then cryopreserved as spores in 25% glycerol and stored at -80 °C. The impact of the cryopreservation method and the storage conditions on the viability of the isolates was assessed after six months of storage and it was established that the isolates were still viable and that the method was adequate. The bioremediation potential of the consortium was further evaluated using a 17 L Pilot scale fluidised-bed bioreactor. The reactors were fed at three different flow rates of 1.5 L.h-1, 2 L.h-1 and 3 L.h-1 over steady state conditions (~3months). The results showed that the FBBR augmented with the selected Bacillus isolates, resulted in improved nutrient (COD, ammonium and phosphates) removal efficiencies compared to the non-bioaugmented control. The highest ammonium removal (62.8%) was observed at a flow rate of 1.5 L.h-1 (11.30 h retention time), whereby there was an overall 29.8% improvement in ammonia removal in comparison to the non-augmented control. Similarly, an overall improvement in phosphate (14.73%) was observed at a flow rate of 2 L.h-1 (8.48 h retention time) with 50% removal efficiency. The highest COD removal was observed at a flow rate of 1.5 L.h-1 (11.30 h retention time) whereby 74.5% COD was reduced with a 32.6% improvement when compared to the non-bioaugmented control. Our work has demonstrated the potential application of Bacillus as bioaugmentation agents to enhance wastewater treatment efficiency as a potential solution to water challenges in developing countries. This technology could also be utilized for addressing the challenges of a wider range of different effluents.


2019 ◽  
Author(s):  
Judit Canellas ◽  
Ana Soares ◽  
Bruce Jefferson

One of the biggest challenges to implementing ion exchange processes in full scale at wastewater treatment works is regeneration of the media. In this paper, regeneration efficiency as a function of brine type, brine reuse, molarity and pH was investigated for the zeolite MesoLite treating synthetic solutions of ammonium. When pretreating the zeolite with KCl rather than NaCl, a 30% improvement in regeneration efficiency was found in the first cycle, which dropped to a 10% improvement in the fifth cycle. For both systems, the observed uptake capacity during the load cycle remained constant, indicating that both were effective and that the brine could be reused five times without deterioration in the performance of the zeolite. The use of KCl was more effective at lower molarities than NaCl such that equivalent regeneration efficiencies were observed at 1.0M and 0.1M for NaCl and KCl respectively. Alteration of the pH between 9 and 12 had no impact on the regeneration efficiency. However, operation at pH 12 was possible without brine. Taken together, these findings indicate that the choice of regenerant can have significant implications on regeneration efficiency and that potassium chloride might be a potentially viable alternative choice.


Author(s):  
Nolwenn Prado ◽  
Juan Ochoa ◽  
Jean-Luc Audic ◽  
Abdeltif Amrane ◽  
Jens Meinhold

The performances of a semi-industrial-scale membrane bioreactor to treat dilute swine wastewater were investigated on a 3 month running period. The treated water was directly reused in the piggery for flushing purposes. The pilot was proved to have good performances in terms of ammonium removal (99%), and COD removal (86%), despite an accumulation of heavy metals in the biological tanks (up to 17 mg/L for zinc and up to 115 mg/L for copper). 79% of the total phosphorus was eliminated through the process, 61% via centrifugation and 18% by biological uptake. The pilot showed a good adaptability to the variations of the loading rates of dilute swine wastewater's major components (COD, TN, TP, heavy metals).


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