Evaluation of the Effect of Betapropiolactone/Ultraviolet Irradiation (BPL/UV) Treatment of Source Plasma on Hepatitis Transmission by Factor IX Complex in Chimpanzees

1982 ◽  
pp. 49-60
Author(s):  
A. M. Prince ◽  
W. Stephan ◽  
B. Brotman ◽  
M. C. Van den Ende
Keyword(s):  
Ultraviolet Irradiation ◽  
Factor Ix ◽  
Uv Treatment

Evaluation of the Effect of Betapropiolactone/Ultraviolet Irradiation (BPL/UV) Treatment of Source Plasma on Hepatitis Transmission by Factor IX Complex in Chimpanzees

1980 ◽  
Vol 44 (03) ◽  
pp. 138-142 ◽  
Author(s):  
Alfred M Prince ◽  
W Stephan ◽  
B Brotman ◽  
M C van den Ende
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Incubation Period ◽  
Factor Ix ◽  
Virus Infectivity ◽  
Uv Treatment ◽  
Plasma Pool ◽  
B Virus ◽  
Complex Preparation ◽  
Liver Biopsies

SummaryTo evaluate the safety of a β-Propiolactone/Ultraviolet (BPL/UV), irradiated Factor IX complex preparation we inoculated 8 chimpanzees with 25 units Factor IX/Kilo from a pool of 5 production lots which had been treated in this manner. These lots were derived from approximately 1,000 donors. Animals were followed with weekly tests for hepatitis B serologic markers and transaminases, and biweekly liver biopsies, for 6 months. No evidence of transmission of hepatitis B, or non-A, non-B viruses was observed.To further evaluate the BPL/UV procedure a plasma pool was intentionally contaminated with hepatitis B virus and one half of the pool treated with BPL/UV. Factor IX complex was isolated from the treated and untreated pools and each was inoculated into 4 chimpanzees. The Factor IX derived from untreated plasma infected all four animals with an average incubation period of 10.5 weeks, whereas that prepared from BPL/UV treated plasma infected only one of four animals with an incubation period of 21 weeks. These results were interpreted as suggesting that BPL/UV can inactivate approximately 99.9% of hepatitis B virus infectivity.

scholarly journals UV treatment of agricultural plants in territories subject to salination of soil

2021 ◽  
Vol 939 (1) ◽  
pp. 012087
Author(s):  
A Anarbaev ◽  
O Tursunov ◽  
D Kodirov ◽  
U Tasheva ◽  
Q Davronov ◽  
...  
Keyword(s):  
Ultraviolet Irradiation ◽  
Relative Yield ◽  
Moisture Capacity ◽  
Uv Treatment ◽  
Technological Basis ◽  
Field Crops ◽  
Agricultural Plants ◽  
And Control ◽  
Experimental Researches ◽  
Irrigated Soils

Abstract An assessment of the influence of electrotechnology of ultraviolet irradiation of seeds and pre-sowing soil treatment and the creation, on their technological basis, of management and control of salt and nutritional regimes of irrigated soils has been carried out. Relative yield of field crops versus the concentration of toxic salts in the soil solution at full moisture capacity are shown. By results of experimental researches acting of UV irradiation on the amount of ammonifying bacteria in the soil under cotton for increasing the accumulation of the most mobile nitrate forms of nitrogen are defined.

Long-Term Tolerance and Recovery of β-Propiolactone/Ultraviolet (βPL/UV) Treated PPSB in Chimpanzees

1981 ◽  
Vol 46 (02) ◽  
pp. 511-514 ◽  
Author(s):  
W Stephan ◽  
R Kotitschke ◽  
A M Prince ◽  
B Brotman
Keyword(s):  
Blood Pressure ◽  
Hepatitis B ◽  
Skin Testing ◽  
Coagulation Factor ◽  
Factor Ix ◽  
Factor Activity ◽  
Uv Treatment

SummaryRecent experiments have shown that a preparation of PPSB (factor IX concentrate) derived from βPL/UV treated plasma was not infectious in chimpanzees with respect to hepatitis B and non-A, non-B. To answer the question whether the βPL/UV treatment influences the tolerance and efficacy of the PPSB concentrate, long-term application of PPSB-Biotest was carried out in chimpanzees. After 10 applications of 25 U factor IX/kg at weekly intervals, no signs of intolerance were observed by measurement of blood pressure during i. v. application and by means of skin-testing. Determination of coagulation factor activity during the application period revealed the same factor IX recovery at the beginning and at the end of the study.

Study On Long Term Tolerance And Recovery Of Cold Sterilized Ppsb In Chimpanzees

1981 ◽  
Author(s):  
W Stephan ◽  
A M Prince ◽  
R Kotitschke
Keyword(s):  
Blood Pressure ◽  
Hepatitis B ◽  
Coagulation Factor ◽  
Factor Ix ◽  
Factor Activity ◽  
Uv Treatment

Recent experiments have shown, that PPSB (factor IX-concentrate) derived from ß-propiolactone/ultraviolet (ß-PL/UV)-treated (cold sterilized)plasma is not infectious in chimpanzees in respect to hepatitis B and Non A-Non B. To answer the question whether the B-PL/UV treatment influences the tolerance and efficacy of the cold sterilized PPSB- concentrate, long term application of PPSB-Biotest was performed in chimpanzees.After 12 applications of 25 units factor IX/kg in weekly intervals no signs of intolerance were observed by means of skintesting and observation of blood pressure during i.v. application. Determination of coagulation factor activity during the application period shows the same factor IX- recovery at the beginning and at the end of the study.

scholarly journals Influence of ultraviolet irradiation of perennial grasses seeds on their sowing quality

2021 ◽  
pp. 97-100
Author(s):  
Zh. S. Nelyubina ◽  
N. I. Kasatkina
Keyword(s):  
Ultraviolet Light ◽  
Ultraviolet Irradiation ◽  
Root Length ◽  
Seedling Development ◽  
Perennial Grasses ◽  
Morphological Parameters ◽  
Seed Infection ◽  
Uv Treatment ◽  
Seed Hardness ◽  
Sowing Seed

A new direction in pre-sowing seed preparation is environmentally friendly and energyefficient ultraviolet irradiation, which increases germination, reduces seed infection, and improves growth force. In this regard, the experiment of studying the effect of UV irradiation on the sowing qualities and morphophysiological parameters of the seeds of the birds-foot trefoil and awnless brome was laid at the UdmFRC UB RAS in 2019. Use of UV rays of range A with a wavelength of 315–400 nm for 5 min reduced the infection of the birds-foot trefoil seeds by 3.2%, increased the length of the sprout by 0.81 cm and the strength of growth — by 5.4% in comparison with untreated seeds. There was also a tendency for a decrease in seed hardness by 2.5%, an increase in root length by 0.41 cm, and the degree of seedling development by 0.12 points. The most effective for awnless brome seeds was UV treatment for 35 minutes, which led to an increase in germination by 14.5%, a decrease in seed infection by 5.8%. There was also a tendency to improve the morphological parameters of seedlings: the length of the sprout increased by 0.9 cm, the length of the root — by 0.88 cm, the length of the coleoptile — by 0.30 cm. The strength of growth in this variant was 9.3% higher than in the control without treatment. In general, the treatment of perennial grasses seeds with ultraviolet light reduced infection, increased laboratory germination and strength of growth, and reduced seed hardness.

Very Low Activated Factor VII and Reduced Factor VII Antigen in Familial Abetalipoproteinaemia

1998 ◽  
Vol 80 (08) ◽  
pp. 233-238 ◽  
Author(s):  
K. A. Mitropoulos ◽  
M. N. Nanjee ◽  
D. J. Howarth ◽  
J. C. Martin ◽  
M. P. Esnouf ◽  
...  
Keyword(s):  
Plasma Concentrations ◽  
Positive Association ◽  
Factor Ix ◽  
Factor Vii ◽  
Unesterified Fatty Acid ◽  
Factor Xii ◽  
Factor X ◽  
Factor Xi ◽  
Activated Factor Vii ◽  
Normal Mean

SummaryAbetalipoproteinaemia is a rare disorder of apolipoprotein B metabolism associated with extremely low plasma concentrations of triglyce-ride. To discover whether the general positive association between factor VII and triglyceride levels extends to this condition, 5 patients were compared with 18 controls. All patients had a triglyceride below 100 μmol/l. Plasma unesterified fatty acid concentration was normal. Although factor IX activity was only slightly reduced (mean 88% standard) and factor IX antigen was normal, mean activated factor VII in patients was strikingly reduced to 34% of that in controls, a level similar to that found in haemophilia B. The patients’ mean factor VII activity and factor VII antigen were also significantly reduced to 54% and 63% of those in controls, respectively. Mean factor XI activity and tissue factor pathway inhibitor activity were reduced in patients to 70% and 75% of control values respectively, while factor XII, factor XI antigen, factor X, prothrombin and protein C were normal.

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