scholarly journals The Soil Habitat and Considerations for Synthetic Biology

2021 ◽  
pp. 169-175
Author(s):  
Robyn A. Barbato
Keyword(s):  
Synthetic Biology ◽  
Natural Environment ◽  
Regulatory Approval ◽  
Ecological Consequences ◽  
Soil System ◽  
Insight Into ◽  
Soil Habitat

AbstractMicroorganisms serve as agents for synthetic biology. It certain instances, the technology is reliant on survival of the genetically-altered microorganism in the natural environment. This chapter offers insight into the soil system to improve the performance of genetically-altered microorganisms in the natural environment. This chapter covers the soil system, the fitness of genetically altered organisms, and considerations for their survival and proliferation in nature. This chapter does not discuss the ecological consequences of survival in the natural environment or the regulatory considerations to distribute a genetically-altered organism in the environment. In addition to regulatory approval, both considerations should be critically reviewed before a genetically-altered organism is distributed in the environment.

Susceptibility of the Heat-, Acid-, and Bile-Adapted Vibrio vulnificus to Lethal Low-Salinity Stress

2006 ◽  
Vol 69 (12) ◽  
pp. 2924-2928 ◽  
Author(s):  
HIN-CHUNG WONG ◽  
SHU-HUI LIU
Keyword(s):  
Stationary Phase ◽  
Salinity Stress ◽  
Natural Environment ◽  
Food Processing ◽  
Vibrio Vulnificus ◽  
Pathogenic Bacterium ◽  
Protective Response ◽  
Low Salinity ◽  
Salinity Challenge ◽  
Insight Into

As a marine pathogenic bacterium that inhabits seawater or seafood, Vibrio vulnificus encounters low salinity and other stresses in the natural environment and during food processing. This investigation explores the cross-protective response of sublethal heat-, acid-, or bile-adapted V. vulnificus YJ03 against lethal low-salinity stress. Experimental results reveal that the acid (pH 4.4)– and heat (41°C)–adapted V. vulnificus were not cross-protected against the lethal low-salinity challenge (0.04% NaCl). The bile (0.05%)–adapted exponential- and stationary-phase cells were cross-protected against low salinity, whereas low-salinity (0.12% NaCl)–adapted stationary cells were sensitized against 12% bile stress. Results of this study provide further insight into the interaction between low salinity and other common stresses in V. vulnificus.

To the Editor

2021 ◽  
pp. 74-92
Author(s):  
Talia Dan-Cohen
Keyword(s):  
Synthetic Biology ◽  
Scientific Work ◽  
Anthropological Perspective ◽  
Unsuccessful Attempt ◽  
Peer Reviews ◽  
Knowledge Practices ◽  
Grant Applications ◽  
General Scientific ◽  
Modern Knowledge ◽  
Insight Into

This chapter unpacks an unsuccessful attempt to publish a key paper from a highly respected peer-reviewed journal devoted to noteworthy and general scientific work. It analyzes the rebuttal from an anthropological perspective, which constitutes a rare documentary artifact of modern knowledge practices. It also explains how the rebuttal provides some insight into the ways authors read and interpret peer reviews at face value, an insight that must be filtered through some general communicative constraints. The chapter refers to the Academics in many fields, such as chemistry and synthetic biology, who write for different readers and adopt different styles and genres. It highlights fairly practiced affairs of papers, review articles, grant applications, peer reviews, tenure letters, and recommendation letters.

Conversation Analysis

2020 ◽  
pp. 764-779
Author(s):  
Maren Rüsch
Keyword(s):  
Conversation Analysis ◽  
Natural Environment ◽  
Natural Speech ◽  
Mutual Understanding ◽  
African Languages ◽  
Sequence Organization ◽  
Basic Concepts ◽  
The 1960S ◽  
Linguistic Approaches ◽  
Insight Into

Conversation analysis, which began to evolve in the 1960s, studies the structure of talk, and how speakers organize a mostly fluent talk without many gaps or overlaps in order to guarantee maximal mutual understanding. It is based on the analysis of natural speech in a culturally natural environment. In this chapter, basic concepts of conversation analysis as well as the methods used by scholars are explained. A collection of examples from several African languages illustrates terminologies such as turn-taking strategies, sequence organization, and repair of trouble-sources in talk and provides an insight into new linguistic approaches to conversation analysis, especially in African settings.

scholarly journals A Synthetic Microbial Operational Amplifier

2017 ◽  
Author(s):  
Ji Zeng ◽  
Jaewook Kim ◽  
Areen Banerjee ◽  
Rahul Sarpeshkar
Keyword(s):  
Transcription Factor ◽  
Synthetic Biology ◽  
Operational Amplifier ◽  
Biological Systems ◽  
Logic Gates ◽  
Living Cells ◽  
Output Stage ◽  
Cellular Circuits ◽  
Differential Transcription ◽  
Insight Into

AbstractSynthetic biology has created oscillators, latches, logic gates, logarithmically linear circuits, and load drivers that have electronic analogs in living cells. The ubiquitous operational amplifier, which allows circuits to operate robustly and precisely has not been built with bio-molecular parts. As in electronics, a biological operational-amplifier could greatly improve the predictability of circuits despite noise and variability, a problem that all cellular circuits face. Here, we show how to create a synthetic 3-stage inducer-input operational amplifier with a differential transcription-factor stage, a CRISPR-based push-pull stage, and an enzymatic output stage with just 5 proteins including dCas9. Our ‘Bio-OpAmp’ expands the toolkit of fundamental circuits available to bioengineers or biologists, and may shed insight into biological systems that require robust and precise molecular homeostasis and regulation.One Sentence SummaryA synthetic bio-molecular operational amplifier that can enable robust, precise, and programmable homeostasis and regulation in living cells with just 5 protein parts is described.

scholarly journals Emplaced writing figuring as a manifestation of an ecocentric mindset in the narrative art of Petra Müller

Literator ◽  
2014 ◽  
Vol 35 (2) ◽  
Author(s):  
Susan Meyer
Keyword(s):  
Natural Environment ◽  
Implied Author ◽  
Narrative Art ◽  
The Core ◽  
The Earth ◽  
Definite Sense ◽  
Earth Connection ◽  
Intellectual Level ◽  
Insight Into ◽  
The Way

The human-earth connection is a sustained theme in Petra Müller’s oeuvre. The article focuses on this connection as reflected in her narrative art, specifically in accounts that have an autobiographical proclivity. The aim of this article is to outline the nature-centred disposition of Müller’s narrative art in a more definite sense. This is achieved by paying attention to the manner in which the author (the ‘I’ in accounts where the narrator can be identified as the author herself) becomes part of the natural environment – whether on a sensory, an emotive, or an intellectual level – where she finds herself and the way she responds to it. At the core of the investigation are the ways in which this reactive engagement is manifested in Müller’s prose work by the implied author and the technique ofemplaced writing. Emplaced writing, a concept created by Linda Russo, was integrated by Susan Smith with Lawrence Buell’s concept of emplacement. This term refers to the technique allowing an active awareness of self and the place physically occupied by the author, as well as how that body fits into this place, to find expression. A broader perspective and greater appreciation of Müller’s work are drawn from the insight into how her close coexistence with the earth is reflected in her narrative art by means of the technique of emplaced writing which is explored in this article as it gives voice to a strong ecocentric disposition.

Introductory Concepts

2014 ◽  
Author(s):  
Domitilla Del Vecchio ◽  
Richard M. Murray
Keyword(s):  
Systems Biology ◽  
Synthetic Biology ◽  
Short Review ◽  
Feedback Systems ◽  
Analysis And Design ◽  
Modeling Analysis ◽  
Key Concepts ◽  
And Control ◽  
Insight Into

This chapter provides a brief introduction to concepts from systems biology; tools from differential equations and control theory; and approaches to the modeling, analysis, and design of biomolecular feedback systems. It begins with a discussion of the role of modeling, analysis, and feedback in biological systems. This is followed by a short review of key concepts and tools from control and dynamical systems theory, which is intended to provide insight into the main methodology described in this volume. Finally, this chapter gives another brief introduction—this time to the field of synthetic biology, which is the primary topic of the latter portion of this book.

Synthetic biology approaches in cancer immunotherapy, genetic network engineering, and genome editing

2016 ◽  
Vol 8 (4) ◽  
pp. 504-517 ◽  
Author(s):  
Deboki Chakravarti ◽  
Jang Hwan Cho ◽  
Benjamin H. Weinberg ◽  
Nicole M. Wong ◽  
Wilson W. Wong
Keyword(s):  
Synthetic Biology ◽  
Cancer Immunotherapy ◽  
Genome Editing ◽  
Genetic Network ◽  
Network Engineering ◽  
Insight Into

Investigations into cells and their contents have provided evolving insight into the emergence of complex biological behaviors.

scholarly journals Reflections and Implications of Philately in the Promotion of Mining and Mineral Samples, Fossils and Gems in Romania (I): 1945-1960

2019 ◽  
pp. 1-20
Author(s):  
Bogdan Cioruța ◽  
Alexandru Leonard Pop ◽  
Mirela Coman
Keyword(s):  
Natural Environment ◽  
Mining Activity ◽  
Future Generations ◽  
Post Office ◽  
Natural Heritage ◽  
Ecological Economic ◽  
History Of ◽  
Historic Significance ◽  
Insight Into ◽  
Over Time

The natural heritage is defined and accepted as the set of physico-geographic and biocenotic (floristic, faunistic) components and structures of the natural environment, whose ecological, economic, scientific, biogenic, sanogenic, landscape, recreational and cultural-historic significance and value has a relevant significance for present and future generations. Constantly promoting philatelic themes that address the natural heritage, different philatelic associations and post office entities performs a series of postage elements in whose pictures we also find the Romanian industrial preoccupations over time. In this paper we propose an insight into the history of mining-philately and bring into discussion the first significant concerns in promoting mining activity and mineral samples, fossils and gems in Romania. In this context, the main objective of this paper is to identify, index and describe the main philatelic pieces issued in Romania (postal stamps, FDCs, occasional envelopes, postcards and maxicards etc), from the beginning of issuing in this field, namely the period 1945-1960.

scholarly journals Životopis i bibliografija prof. dr. sc. Pavuše Vežića

Ars Adriatica ◽  
2017 ◽  
Vol 7 (1) ◽  
pp. 9
Author(s):  
Emil Hilje
Keyword(s):  
Early Modern ◽  
Faculty Member ◽  
Natural Environment ◽  
Art History ◽  
Direct Contact ◽  
Early Christian ◽  
Academic Achievements ◽  
History Department ◽  
Artistic Heritage ◽  
Insight Into

Pavuša Vežić, eminent Zadar art historian and heritage conservationist was born in 1947 in Makarska. After graduating art history at former Faculty of Philosophy in Zadar, he worked on a number of important conservationist campaigns in Zadar, such as the rotund of St. Donatus, basilica of St. Thomas', Nassis, house, Citadel fort, St. Stephen's basilica, Zadar cathedral as well as on a number of important mediaeval and early modern buildings in Zadar region. These conservation and protection campaigns were accompanied by a number of academic achievements since direct contact with heritage had become the natural environment for research and insight into number of problems and questions that resulted with new comprehension and interpretations. Along with considerable conservationist career, Vežić received his PhD in 1994 at the Faculty of Philosophy in Zadar with thesis entitled The Episcopal complex in Zadar. In 1999, he joined Zadar art history Department as faculty member, where he finally received tenure in 2012. Professor Vežić's academic focus is in the early Christian and early mediaeval architecture as well as on questions of conservation and protection of heritage, in which fields he had published a number of widely recognized monographs, scientific and professional articles. Vežić's lectures are also affectionately remembered by generations of his students as well as citizens of Zadar who attended his public discourses on a variety of topics related to Zadar architectural and artistic heritage.

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