Plant Virus Diagnostics: Traditional to Recent and Emerging Advances

2020 ◽  
pp. 97-111
Author(s):  
V. K. Baranwal ◽  
Sajad Un Nabi ◽  
Manoj K. Yadav
Keyword(s):  
Plant Virus ◽  
Virus Diagnostics

scholarly journals Application of Oxford Nanopore Technology to Plant Virus Detection

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1424
Author(s):  
Lia W. Liefting ◽  
David W. Waite ◽  
Jeremy R. Thompson
Keyword(s):  
Plant Virus ◽  
High Throughput Sequencing ◽  
Virus Detection ◽  
Diagnostic Methods ◽  
Plant Virus Detection ◽  
Sequencing Technologies ◽  
Oxford Nanopore ◽  
Virus Diagnostics ◽  
Post Entry ◽  
Read Accuracy

The adoption of Oxford Nanopore Technologies (ONT) sequencing as a tool in plant virology has been relatively slow despite its promise in more recent years to yield large quantities of long nucleotide sequences in real time without the need for prior amplification. The portability of the MinION and Flongle platforms combined with lowering costs and continued improvements in read accuracy make ONT an attractive method for both low- and high-scale virus diagnostics. Here, we provide a detailed step-by-step protocol using the ONT Flongle platform that we have developed for the routine application on a range of symptomatic post-entry quarantine and domestic surveillance plant samples. The aim of this methods paper is to highlight ONT’s feasibility as a valuable component to the diagnostician’s toolkit and to hopefully stimulate other laboratories towards the eventual goal of integrating high-throughput sequencing technologies as validated plant virus diagnostic methods in their own right.

scholarly journals Current impact and future directions of high throughput sequencing in plant virus diagnostics

2014 ◽  
Vol 188 ◽  
pp. 90-96 ◽  
Author(s):  
Sebastien Massart ◽  
Antonio Olmos ◽  
Haissam Jijakli ◽  
Thierry Candresse
Keyword(s):  
High Throughput ◽  
Plant Virus ◽  
High Throughput Sequencing ◽  
Future Directions ◽  
Virus Diagnostics ◽  
Current Impact

scholarly journals Evolution of Plant Virus Diagnostics Used in Australian Post Entry Quarantine

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1430
Author(s):  
Mark Whattam ◽  
Adrian Dinsdale ◽  
Candace E. Elliott
Keyword(s):  
Plant Virus ◽  
Diagnostic Techniques ◽  
Molecular Techniques ◽  
Special Edition ◽  
Australian Government ◽  
Virus Diagnostics ◽  
Diagnostic Approaches ◽  
Post Entry ◽  
High Level ◽  
Safe Access

As part of a special edition for MDPI on plant virology in Australia, this review provides a brief high-level overview on the evolution of diagnostic techniques used in Australian government Post-Entry Quarantine (PEQ) facilities for testing imported plants for viruses. A comprehensive range of traditional and modern diagnostic approaches have historically been employed in PEQ facilities using bioassays, serological, and molecular techniques. Whilst these techniques have been effective, they are time consuming, resource intensive and expensive. The review highlights the importance of ensuring the best available science and diagnostic developments are constantly tested, evaluated, and implemented by regulators to ensure primary producers have rapid and safe access to new genetics to remain productive, sustainable and competitive.

scholarly journals CRISPR Applications in Plant Virology: Virus Resistance and Beyond

2020 ◽  
Vol 110 (1) ◽  
pp. 18-28 ◽  
Author(s):  
Natalia O. Kalinina ◽  
Andrey Khromov ◽  
Andrew J. Love ◽  
Michael E. Taliansky
Keyword(s):  
Virus Resistance ◽  
Plant Virus ◽  
Plant Protection ◽  
Adaptive Immune System ◽  
Host Susceptibility ◽  
Virus Infections ◽  
Basic Principles ◽  
Plant Virology ◽  
Efficient Gene ◽  
Virus Diagnostics

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated genes (Cas) is a prokaryotic adaptive immune system which has been reprogrammed into a precise, simple, and efficient gene targeting technology. This emerging technology is revolutionizing various areas of life sciences, medicine, and biotechnology and has raised significant interest among plant biologists, both in basic science and in plant protection and breeding. In this review, we describe the basic principles of CRISPR/Cas systems, and how they can be deployed to model plants and crops for the control, monitoring, and study of the mechanistic aspects of plant virus infections. We discuss how Cas endonucleases can be used to engineer plant virus resistance by directly targeting viral DNA or RNA, as well as how they can inactivate host susceptibility genes. Additionally, other applications of CRISPR/Cas in plant virology such as virus diagnostics and imaging are reviewed. The review also provides a systemic comparison between CRISPR/Cas technology and RNA interference approaches, the latter of which has also been used for development of virus-resistant plants. Finally, we outline challenges to be solved before CRISPR/Cas can produce virus-resistant crop plants which can be marketed.

scholarly journals Application of HTS for Routine Plant Virus Diagnostics: State of the Art and Challenges

2018 ◽  
Vol 9 ◽  
Author(s):  
Hans J. Maree ◽  
Adrian Fox ◽  
Maher Al Rwahnih ◽  
Neil Boonham ◽  
Thierry Candresse
Keyword(s):  
Plant Virus ◽  
State Of The Art ◽  
Virus Diagnostics

Search mode for rapid detection of virus particles

1991 ◽  
Vol 49 ◽  
pp. 286-287
Author(s):  
O. E. Bradfute
Keyword(s):  
Electron Microscopy ◽  
Rapid Detection ◽  
Plant Virus ◽  
Low Dose ◽  
Search Mode ◽  
Virus Diseases ◽  
Virus Particles ◽  
Focus Image ◽  
Time Required ◽  
Rapid Switching

Electron microscopy is frequently used in preliminary diagnosis of plant virus diseases by surveying negatively stained preparations of crude extracts of leaf samples. A major limitation of this method is the time required to survey grids when the concentration of virus particles (VPs) is low. A rapid survey of grids for VPs is reported here; the method employs a low magnification, out-of-focus Search Mode similar to that used for low dose electron microscopy of radiation sensitive specimens. A higher magnification, in-focus Confirm Mode is used to photograph or confirm the detection of VPs. Setting up the Search Mode by obtaining an out-of-focus image of the specimen in diffraction (K. H. Downing and W. Chiu, private communications) and pre-aligning the image in Search Mode with the image in Confirm Mode facilitates rapid switching between Modes.

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