Unravelling Mosquito Species Complex Through DNA Barcodes: Complementing Morphological Identification for Accurate Discrimination

2020 ◽  
pp. 1-21
Author(s):  
Deepika Panda ◽  
Tapan Kumar Barik
Keyword(s):  
Species Complex ◽  
Mosquito Species ◽  
Morphological Identification ◽  
Dna Barcodes

DNA Barcoding of Simulium asakoae (Diptera: Simuliidae) From Northern Thailand

2020 ◽  
Vol 57 (5) ◽  
pp. 1675-1678 ◽  
Author(s):  
Van Lun Low ◽  
Wichai Srisuka ◽  
Atiporn Saeung ◽  
Tiong Kai Tan ◽  
Zubaidah Ya’cob ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Barcoding ◽  
Genetic Divergence ◽  
Species Complex ◽  
Morphological Variability ◽  
Single Species ◽  
Genetic Distances ◽  
Morphological Identification ◽  
Dna Barcodes ◽  
Barcode Gap

Abstract Previous studies suggested the presence of species complex in the so-called Simulium asakoae Takaoka & Davies (Diptera: Simuliidae) in Thailand due to its high morphological variability and genetic divergence. To investigate whether the true S. asakoae is present in Thailand, we performed a detailed morphological identification of S. asakoae and compared its DNA barcodes with the morphospecies S. asakoae from Myanmar and the typical S. asakoae from Malaysia. Phylogenetic analysis revealed the Thai materials analyzed in this study were indeed genetically similar with those from Myanmar and Malaysia, though genetic distances 0–2.27% were observed. We tentatively regard this divergence as intraspecific variation, and the automatic barcode gap discovery analysis further supports them as a single species.

scholarly journals DNA barcode identification of commercial fish sold in Mexican markets

Genome ◽  
2018 ◽  
Vol 61 (6) ◽  
pp. 457-466 ◽  
Author(s):  
Stephanie Sarmiento-Camacho ◽  
Martha Valdez-Moreno
Keyword(s):  
Conservation Status ◽  
Dna Barcode ◽  
Common Species ◽  
Morphological Identification ◽  
Dna Barcodes ◽  
Commercial Fish ◽  
Sphyrna Lewini ◽  
Pangasianodon Hypophthalmus ◽  
Makaira Nigricans ◽  
The Status

The substitution of high-value fish species for those of lower value is common practice. Although numerous studies have addressed this issue, few have been conducted in Mexico. In this study, we sought to identify fresh fillets of fish, sharks, and rays using DNA barcodes. We analyzed material from “La Viga” in Mexico City, and other markets located on the Gulf and Caribbean coasts of Mexico. From 134 samples, we obtained sequences from 129, identified to 9 orders, 28 families, 38 genera, and 44 species. The most common species were Seriola dumerili, Pangasianodon hypophthalmus, Carcharhinus falciformis, Carcharhinus brevipinna, and Hypanus americanus. Pangasianodon hypophthalmus was most commonly used as a substitute for higher-value species. The substitution rate was 18% of the total. A review of the conservation status of the specimens identified against the IUNC list enabled us to establish that some species marketed in Mexico are threatened: Makaira nigricans, Lachnolaimus maximus, Hyporthodus flavolimbatus, and Isurus oxyrinchus are classified as vulnerable; Lopholatilus chamaeleonticeps and Sphyrna lewini are endangered; and the status of Hyporthodus nigritus is critical. These results will demonstrate to the Mexican authorities that DNA barcoding is a reliable tool for species identification, even when morphological identification is difficult or impossible.

DNA Barcodes indicate members of the Anopheles fluviatilis (Diptera: Culicidae) species complex to be conspecific in India

2013 ◽  
Vol 13 (3) ◽  
pp. 354-361 ◽  
Author(s):  
N. Pradeep kumar ◽  
N. Krishnamoorthy ◽  
S. S. Sahu ◽  
A. R. Rajavel ◽  
S. Sabesan ◽  
...  
Keyword(s):  
Species Complex ◽  
Dna Barcodes ◽  
Anopheles Fluviatilis

DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae)

2014 ◽  
Vol 104 (4) ◽  
pp. 486-493 ◽  
Author(s):  
Y.J. Wang ◽  
Z.H. Li ◽  
S.F. Zhang ◽  
Z. Varadínová ◽  
F. Jiang ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Dna Sequences ◽  
Morphological Identification ◽  
Pest Species ◽  
Dna Barcodes ◽  
Molecular Approaches ◽  
Worldwide Distribution ◽  
Haplotype Networks ◽  
Stored Product Pest ◽  
The Usa

AbstractSeveral species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schönherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel & Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658 bp fragment from the mitochondrial DNA cytochrome c oxidase subunit I (COI) was adopted to overcome some problems of morphological identification of Cryptolestes species. The utility of COI sequences as DNA barcodes in discriminating the five Cryptolestes species was evaluated on adults and larvae by analysing Kimura 2-parameter distances, phylogenetic tree and haplotype networks. The results showed that molecular approaches based on DNA barcodes were able to accurately identify these species. This is the first study using DNA barcoding to identify Cryptolestes species and the gathered DNA sequences will complement the biological barcode database.

Culicoides Latreille biting midges (Diptera: Ceratopogonidae) of the Dongzhaigang mangrove forest, Hainan Province, China

Zootaxa ◽  
2017 ◽  
Vol 4227 (1) ◽  
pp. 49
Author(s):  
JIA-HUI LI ◽  
DAVID GOPURENKO ◽  
DU-CHENG CAI ◽  
YE-MENG YANG ◽  
RONG HU ◽  
...  
Keyword(s):  
Mangrove Forest ◽  
New Records ◽  
Light Trap ◽  
Morphological Identification ◽  
Hainan Province ◽  
Dna Barcodes ◽  
Biting Midges ◽  
Biting Midge ◽  
Human Landing ◽  
Sweep Net

The biting midge fauna of Dongzhaigang Mangrove Forest, Hainan Province, China was sampled on 14 October 2015 using three methods: a pan light trap operated from dusk until dawn the following morning and sweep net and human landing collections performed between 16:15–17:15 hr. Eight species, including two new records for China, Culicoides palawanensis and C. niphanae, and one new record for Hainan, C. circumbasalis, were collected. A key to assist with identification of specimens of these species is provided. DNA barcodes supported the morphological identification of some of these species and identified the potential presence of cryptic species and/or deep population structure in others. The newly recorded species were morphologically similar to species previously reported from Hainan, highlighting the need for further investigation into the taxonomy of biting midges in this region. Species composition and abundance varied considerably between the three collection techniques suggesting that multiple techniques likely provide a more comprehensive sample of biting midge fauna. 

scholarly journals DNA barcoding: complementing morphological identification of mosquito species in Singapore

2014 ◽  
Vol 7 (1) ◽  
Author(s):  
Abigail Chan ◽  
Lee-Pei Chiang ◽  
Hapuarachchige C Hapuarachchi ◽  
Cheong-Huat Tan ◽  
Sook-Cheng Pang ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Mosquito Species ◽  
Morphological Identification

scholarly journals Human biting Culex pipiens bioform molestus is spread in several areas in south Sweden

2019 ◽  
Author(s):  
Luande Verah Nafula ◽  
Disa Eklöf ◽  
Anders Lindström ◽  
Steven Ger Nyanjom ◽  
Magnus Evander ◽  
...  
Keyword(s):  
Rural Areas ◽  
Urban Areas ◽  
Culex Pipiens ◽  
Mosquito Species ◽  
Urban Environments ◽  
Coi Gene ◽  
Morphological Identification ◽  
Potential Vector ◽  
South Sweden ◽  
Citizen Science Project

AbstractThe mosquito species Culex pipiens is a potential vector of several pathogens infecting humans and occurs in two distinct bioforms, pipiens and molestus. Traditional morphological identification fails to separate the bioforms of Cx. pipiens despite their behavioural differences since they are morphologically indistinguishable. However, molecular methods can identify the two bioforms. The bioform molestus thrives in urban environments and bite all kinds of vertebrates, whereas bioform pipiens is more rural and mainly feed on birds.Mosquito samples submitted in a citizen science project from people experiencing mosquito problems in South Sweden were analyzed to determine the geographical distribution of the molestus bioform of Cx. pipiens. Mosquito specimens were identified to species by DNA barcoding of the cytochrome C oxidase subunit I (COI) gene and the bioforms were determined with the CQ11 microsatellite marker. To establish other differences between the bioforms, part of the CPIJ001674 gene was sequenced. Culex pipiens f molestus, was present both within and outside of urban areas in several sites in southern Sweden. In one site, hybrids between the two bioforms were found. Culex pipiens f molestus has previously been found in urban areas in Sweden, but the detection of the bioform in several rural areas was surprising, indicating that it may be more widely spread than previously thought.

scholarly journals Contribution to the knowledge of the beetle fauna (Insecta, Coleoptera) of Malta: new records of seven species with supporting DNA barcodes

Check List ◽  
2021 ◽  
Vol 17 (5) ◽  
pp. 1443-1449
Author(s):  
Clare Marie Mifsud ◽  
Noel Vella ◽  
Adriana Vella
Keyword(s):  
Dna Barcoding ◽  
New Records ◽  
Morphological Identification ◽  
Dna Barcodes ◽  
Central Mediterranean ◽  
Maltese Islands

We report the presence of seven species of Coleoptera newly found on the Maltese Islands in the central Mediterranean. The first species records from the Maltese Islands include: Ancylopus melanocephalus (Olivier, 1808) (Endomychidae), Aplidia transversa (Fabricius, 1801) (Scarabaeidae), Cercyon quisquilius (Linnaeus, 1761) (Hydrophilidae), Hyperaspis duvergeri Fürsch, 1985 (Coccinellidae), Lebia cruxminor (Linnaeus, 1758) (Carabidae), Smicronyx pauperculus Wollaston, 1864 (Curculionidae), and Oxytelus sculptus Gravenhorst, 1806 (Staphylinidae). The morphological identification of each newly reported species was also confirmed through DNA barcoding.

scholarly journals Update on the mosquito fauna (Diptera: Culicidae) distribution in Cabo Verde: occurrence of the species complexes Anopheles gambiae and Culex pipiens (pipiens, quinquefasciatus and their hybrids)

2021 ◽  
Author(s):  
Silvânia da Veiga Leal ◽  
Isaias Baptista Fernandes Varela ◽  
Davidson Daniel Sousa Monteiro ◽  
Celivianne Marisia Ramos de Sousa ◽  
Maria da Luz Lima Mendonça ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Culex Pipiens ◽  
Mosquito Species ◽  
Morphological Identification ◽  
Widespread Species ◽  
Breeding Sites ◽  
Vector Borne Diseases ◽  
Entomological Survey ◽  
Cabo Verde ◽  
Species Complexes

In this study, we aimed to update the mosquito species composition and distribution based on a national entomological survey in all municipalities of Cabo Verde. This includes the sibling species of the Culex pipiens complex, namely Cx. pipiens , Cx. quinquefasciatus and their hybrids, in locations where information is not available. The entomological survey took place from October 2017 to September 2018, in all municipalities of Cabo Verde. Mosquito larvae and pupae were collected in breeding sites and samples were sent to the Laboratory of Medical Entomology of the National Institute of Public Health for the morphological identification of the species. The mosquitoes morphologically identified in Anopheles gambiae and Culex pipiens complexes of species were further molecular analysed to species confirmation. A total of 814 breeding sites were surveyed and 10 mosquito species of five genera were identified. The greatest number of mosquito species was reported in the island of Santiago. The most widespread species in the country were Aedes aegypti and Culex quinquefasciatus. Anopheles arabiensis was the only species identified in the Gambiae complex of species. The results of this study will assist decision makers in important health policies to control mosquitoes and vector-borne diseases towards a strategic approach by timely detection of changes in species diversity.

scholarly journals A multiplex PCR assay for six Aedini species, including Aedes albopictus

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Woo Jun Bang ◽  
Min Hyeok Won ◽  
Seong Tae Cho ◽  
Jihun Ryu ◽  
Kwang Shik Choi
Keyword(s):  
Multiplex Pcr ◽  
Aedes Albopictus ◽  
Disease Transmission ◽  
Mosquito Species ◽  
Pairwise Distance ◽  
Molecular Diagnostic ◽  
Morphological Identification ◽  
Human Pathogens ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Abstract Background Mosquitoes, as vectors of various human pathogens, are significant drivers of serious human illness. In particular, those species in the Aedini tribe, which typically transmit dengue virus, Chikungunya fever virus, and Zika virus, are increasing their range because of climate change and international commerce. In order to evaluate the risk of disease transmission, accurate mosquito species identification and monitoring are needed. The goal of this work was to develop a rapid and simple molecular diagnostic method for six morphologically similar Aedini species (Aedes flavopictus, Aedes albopictus, Ochlerotatus koreicus, Ochlerotatus japonicus, Ochlerotatus togoi and Ochlerotatus hatorii) in Korea. Methods A total of 109 samples were assayed in this study. The internal transcribed spacer 2 (ITS2) regions from all six species were amplified, sequenced and analyzed using Mega 6. Following the identification of regions that were consistently different in terms of sequence between all six species, multiplex primers were designed to amplify these regions to generate species-specific fragments distinguishable by their size. Results Uniquely sized fragments were generated in Ae. flavopictus (495 bp), Ae. albopictus (438 bp), Oc. koreicus (361 bp), Oc. togoi (283 bp), Oc. hatorii (220 bp) and Oc. japonicus (160 bp). Pairwise distance analysis showed that the difference was 35.0 ± 1.5% between Aedes spp. and Ochlerotatus spp., 17.4 ± 0.2% between Ae. albopictus and Ae. flavopictus and 11.1 ± 0.3% between Oc. koreicus and Oc. japonicus. Conclusions In this study, a multiplex PCR assay for six species of the Aedini tribe was developed. This assay is more accurate than morphological identification and will be useful for monitoring and controlling these vector mosquitoes. Graphical Abstract

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