Culicoides Latreille biting midges (Diptera: Ceratopogonidae) of the Dongzhaigang mangrove forest, Hainan Province, China

Zootaxa ◽  
2017 ◽  
Vol 4227 (1) ◽  
pp. 49
Author(s):  
JIA-HUI LI ◽  
DAVID GOPURENKO ◽  
DU-CHENG CAI ◽  
YE-MENG YANG ◽  
RONG HU ◽  
...  
Keyword(s):  
Mangrove Forest ◽  
New Records ◽  
Light Trap ◽  
Morphological Identification ◽  
Hainan Province ◽  
Dna Barcodes ◽  
Biting Midges ◽  
Biting Midge ◽  
Human Landing ◽  
Sweep Net

The biting midge fauna of Dongzhaigang Mangrove Forest, Hainan Province, China was sampled on 14 October 2015 using three methods: a pan light trap operated from dusk until dawn the following morning and sweep net and human landing collections performed between 16:15–17:15 hr. Eight species, including two new records for China, Culicoides palawanensis and C. niphanae, and one new record for Hainan, C. circumbasalis, were collected. A key to assist with identification of specimens of these species is provided. DNA barcodes supported the morphological identification of some of these species and identified the potential presence of cryptic species and/or deep population structure in others. The newly recorded species were morphologically similar to species previously reported from Hainan, highlighting the need for further investigation into the taxonomy of biting midges in this region. Species composition and abundance varied considerably between the three collection techniques suggesting that multiple techniques likely provide a more comprehensive sample of biting midge fauna. 

scholarly journals Contribution to the knowledge of the beetle fauna (Insecta, Coleoptera) of Malta: new records of seven species with supporting DNA barcodes

Check List ◽  
2021 ◽  
Vol 17 (5) ◽  
pp. 1443-1449
Author(s):  
Clare Marie Mifsud ◽  
Noel Vella ◽  
Adriana Vella
Keyword(s):  
Dna Barcoding ◽  
New Records ◽  
Morphological Identification ◽  
Dna Barcodes ◽  
Central Mediterranean ◽  
Maltese Islands

We report the presence of seven species of Coleoptera newly found on the Maltese Islands in the central Mediterranean. The first species records from the Maltese Islands include: Ancylopus melanocephalus (Olivier, 1808) (Endomychidae), Aplidia transversa (Fabricius, 1801) (Scarabaeidae), Cercyon quisquilius (Linnaeus, 1761) (Hydrophilidae), Hyperaspis duvergeri Fürsch, 1985 (Coccinellidae), Lebia cruxminor (Linnaeus, 1758) (Carabidae), Smicronyx pauperculus Wollaston, 1864 (Curculionidae), and Oxytelus sculptus Gravenhorst, 1806 (Staphylinidae). The morphological identification of each newly reported species was also confirmed through DNA barcoding.

Biting Midges in Jamari National Forest, in the Brazilian Amazon, With 12 New Records of Culicoides Species (Diptera: Ceratopogonidae) for the State of Rondônia

2020 ◽  
Author(s):  
Luis Paulo Costa Carvalho ◽  
Antônio Marques Pereira Júnior ◽  
Felipe Arley Costa Pessoa ◽  
Jansen Fernandes Medeiros
Keyword(s):  
Amazon Basin ◽  
Light Trap ◽  
Brazilian Amazon ◽  
Abundant Species ◽  
National Forest ◽  
Culicoides Species ◽  
Conservation Area ◽  
Biting Midges ◽  
Biting Midge ◽  
Bg Sentinel

Abstract Culicoides biting midges are small hematophagous flies, some species of which transmit pathogens to livestock and humans. In the Brazilian Amazon Basin, Culicoides diversity has remained largely unexamined. Jamari National Forest (JNF) is a conservation area in Rondônia State where research and environmental education are conducted in conjunction with the protection of natural resources, and the recovery of degraded areas. The present study augments our knowledge of Culicoides diversity in JNF. The survey collected 640 individuals from 41 species; 632 individuals were collected by HP light trap and 8 were collected by the BG Sentinel trap. Twelve species are first-time records for Rondônia: Culicoides acotylus Lutz, Culicoides albuquerquei Wirth & Blanton, Culicoides baniwa Felippe-Bauer, Culicoides bricenoi Ortiz, Culicoides brownei Spinelli, Culicoides carsiomelas Wirth & Blanton, Culicoides debilipalpis Lutz, Culicoides forattinii Ortiz, Culicoides ginesi Ortiz, Culicoides profundus Santarém, Felippe-Bauer & Trindade, Culicoides pseudoreticulatus Santarém, Felippe-Bauer & Castellón, and Culicoides spurius Santarém, Felippe-Bauer & Trindade. The most abundant species were Culicoides glabrior Macfie, Culicoides pseudodiabolicus Fox, Culicoides debilipalpis, Culicoides quasiparaensis Clastrier, and Culicoides brownei Spinelli. The present study updates the Culicoides species count in Rondônia to 55, and highlights the importance of JNF as a hotbed for biting midge diversity.

scholarly journals Phylogenetic Complexity of Morphologically Identified Anopheles squamosus in Southern Zambia

Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 146
Author(s):  
Jordan Hoffman ◽  
Ilinca Ciubotariu ◽  
Limonty Simubali ◽  
Twig Mudenda ◽  
William Moss ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Catchment Area ◽  
Control Strategies ◽  
Light Trap ◽  
Current Control ◽  
Control Measures ◽  
Morphological Identification ◽  
The Past ◽  
Blood Meal Source ◽  
Residual Malaria Transmission

Despite dramatic reductions in malaria cases in the catchment area of Macha Hospital, Choma District, Southern Province in Zambia, prevalence has remained near 1–2% by RDT for the past several years. To investigate residual malaria transmission in the area, this study focuses on the relative abundance, foraging behavior, and phylogenetic relationships of Anopheles squamosus specimens. In 2011, higher than expected rates of anthropophily were observed among “zoophilic” An. squamosus, a species that had sporadically been found to contain Plasmodium falciparum sporozoites. The importance of An. squamosus in the region was reaffirmed in 2016 when P. falciparum sporozoites were detected in numerous An. squamosus specimens. This study analyzed Centers for Disease Control (CDC) light trap collections of adult mosquitoes from two collection schemes: one performed as part of a reactive-test-and-treat program and the second performed along a geographical transect. Morphological identification, molecular verification of anopheline species, and blood meal source were determined on individual samples. Data from these collections supported earlier studies demonstrating An. squamosus to be primarily exophagic and zoophilic, allowing them to evade current control measures. The phylogenetic relationships generated from the specimens in this study illustrate the existence of well supported clade structure among An. squamosus specimens, which further emphasizes the importance of molecular identification of vectors. The primarily exophagic behavior of An. squamosus in these collections also highlights that indoor vector control strategies will not be sufficient for elimination of malaria in southern Zambia.

scholarly journals New Records of Anopheles benarrochi B (Diptera: Culicidae) in Malaria Hotspots in the Amazon Regions of Ecuador and Peru

2021 ◽  
Author(s):  
Diego Morales Viteri ◽  
Manuela Herrera-Varela ◽  
Maribel Albuja ◽  
Cristina Quiroga ◽  
Gloria Diaz ◽  
...  
Keyword(s):  
Vector Control ◽  
Malaria Transmission ◽  
New Records ◽  
Final Analysis ◽  
Human Landing Catch ◽  
Light Traps ◽  
Control Units ◽  
Pcr Rflp ◽  
Human Landing ◽  
First Time

Abstract The increase in malaria transmission in the Amazon region motivated vector control units of the Ministry of Health of Ecuador and Peru to investigate Anopheles (Diptera: Culicidae) species present in transmission hotspots. Mosquitoes were collected using prokopack aspirators and CDC light traps (Ecuador) and human landing catch in Peru. In Ecuador, 84 Anopheles were captured from Pastaza, Morona Santiago, and Orellana provinces and identified morphologically [An. (An.) apicimacula Dyar and Knab, An. (Nys.) near benarrochi, An. (Nys.) near oswaldoi, An. (Nys.) near strodei, An. (An.) nimbus (Theobald, 1902), and An. (Nyssorhynchus) sp.]. In Peru, 1,150 Anopheles were collected in Andoas District. A subsample of 166 specimens was stored under silica and identified as An. near oswaldoi, An. darlingi, and An. (An.) mattogrossensis Lutz and Neiva. COI barcode region sequences were obtained for 137 adults (107 from Peru, 30 from Ecuador) identified by ITS2 PCR-RFLP as An. benarrochi Gabaldon, Cova Garcia, and Lopez and retained in the final analysis. Haplotypes from the present study plus An. benarrochi B GenBank sequences grouped separately from Brazilian An. benarrochi GenBank sequences by 44 mutation steps, indicating that the present study specimens were An. benarrochi B. Our findings confirm the presence of An. benarrochi B in Ecuador and reported here for the first time from the Amazonian provinces of Orellana and Morona Santiago. Furthermore, we confirm that the species collected in Andoas District in the Datem del Maranon Province, Peru, is An. benarrochi B, and we observed that it is highly anthropophilic. Overall, the known distribution of An. benarrochi B has been extended and includes southern Colombia, much of Peru and eastern Ecuador.

New records of the occurrence of biting midges (Diptera: Ceratopogonidae) species in rural area of Santa Maria, Rio Grande do Sul, in the South of Brazil

2022 ◽  
Author(s):  
Julia Somavilla Lignon ◽  
Emanuelle de Souza Farias ◽  
Felipe Arley Costa Pessoa ◽  
Eduarda Maria Trentin Santi ◽  
Lucas Alexandre Farias de Souza ◽  
...  
Keyword(s):  
Rural Area ◽  
New Records ◽  
Rio Grande ◽  
Rio Grande Do Sul ◽  
The South ◽  
Biting Midges ◽  
South Of Brazil ◽  
Santa Maria

scholarly journals Two New Records of Upeneus Spottocaudalis and U. Sundaicus (Perciformes: Mullidae) from the Northern South China Sea Based on the Morphology and DNA Barcoding

2021 ◽  
Author(s):  
Zhisen Luo ◽  
Murong Yi ◽  
Kangwen Qiu ◽  
Sibiao Liu ◽  
Sui Gu ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
New Records ◽  
Northern South China Sea ◽  
Genetic Distances ◽  
Dna Barcodes ◽  
China Sea ◽  
Homologous Sequences ◽  
Nj Tree ◽  
Barcode Gap

Abstract In this study, two new records of goatfishes Upeneus spottocaudalis and U. sundaicus from the South China Sea, combing evidence from morphology and DNA barcodes for species identification. ML tree and NJ tree result showed that the sequences of U. spottocaudalis and U. sundaicus were clustered with the homologous sequences form GenBank, respectively, and the intraspecific genetic distances of U. spottocaudalis (0.2%) and U. sundaicus (0.3%) were less than 2%. Automatic Barcode Gap Discovery (ABGD) analysis also supported this result of classification.

scholarly journals A new genus and a new species in the subfamily Polyzosteriinae (Blattodea, Blattidae) from China

ZooKeys ◽  
2019 ◽  
Vol 852 ◽  
pp. 85-100 ◽  
Author(s):  
Shuran Liao ◽  
Zongqing Wang ◽  
Yanli Che
Keyword(s):  
New Species ◽  
Sexual Dimorphism ◽  
New Genus ◽  
Morphological Data ◽  
Hainan Province ◽  
Dna Barcodes ◽  
Guangxi Province ◽  
A New Species

Laevifaciesquadrialatagen. et sp. nov. is described from Hainan Province, China based on morphological data. COI data (DNA barcodes) is utilized to confirm the sexual dimorphism occurring in Laevifaciesquadrialatagen. et sp. nov.Melanozosterianitida Brunner von Wattenwyl, 1865, is reported from Guangxi Province, China. A key to the Chinese Polyzosteriinae is provided.

scholarly journals Morphological identification of phosphate solubilizing and cellulolytic fungi from mangrove soil under Rhizophora stylosa stands

2021 ◽  
Vol 912 (1) ◽  
pp. 012052
Author(s):  
D Elfiati ◽  
Delvian ◽  
A Susilowati ◽  
N W Y Rizki ◽  
A F M Harahap ◽  
...  
Keyword(s):  
Mangrove Forest ◽  
Methyl Cellulose ◽  
Economic Benefits ◽  
Soil Samples ◽  
Morphological Identification ◽  
Rhizophora Stylosa ◽  
Cellulolytic Fungi ◽  
Mangrove Soil ◽  
Phosphate Solubilizing ◽  
North Sumatra

Abstract Mangrove forest is one of the forest ecosystems that can be found in tidal areas. Mangrove forest have ecological, socio-economic benefits for organisms that live in the sea. One of the microorganism that live in mangrove forest is fungus. Therefore, this study aimed to isolate and identify phosphate solubilizing and cellulolytic fungi from mangrove soil. Soil samples were collected from a stands of Rhizophora stylosa in Lubuk Kertang Village, West Brandan District, Langkat Regency, North Sumatra. Soil samples were taken randomly with a dept of 0-20 cm. Isolation of phosphate solubilizing fungi was carried out using Pikovskaya medium and cellulolytic fungi using carboxyl methyl cellulose (CMC) medium. To determine the effectiveness of fungi qualitatively, the phosphate solubility and cellulolytic index were calculated. All isolates obtained were identified morphologically by observing colonies macroscopically and microscopically. This study obtained 12 isolates of phosphate solubilizing and 8 isolates of cellulolytic fungi. Phosphate solubility index ranging from 2.06-2.87 and cellulolytic index of 0.33-3.00. Morphological identifications showed that all isolates of phosphate solubilizing fungi belonged to the genus Aspergillus, while all isolates of cellulolytic fungi belonged to the genus Chaetomium.

scholarly journals DNA barcode identification of commercial fish sold in Mexican markets

Genome ◽  
2018 ◽  
Vol 61 (6) ◽  
pp. 457-466 ◽  
Author(s):  
Stephanie Sarmiento-Camacho ◽  
Martha Valdez-Moreno
Keyword(s):  
Conservation Status ◽  
Dna Barcode ◽  
Common Species ◽  
Morphological Identification ◽  
Dna Barcodes ◽  
Commercial Fish ◽  
Sphyrna Lewini ◽  
Pangasianodon Hypophthalmus ◽  
Makaira Nigricans ◽  
The Status

The substitution of high-value fish species for those of lower value is common practice. Although numerous studies have addressed this issue, few have been conducted in Mexico. In this study, we sought to identify fresh fillets of fish, sharks, and rays using DNA barcodes. We analyzed material from “La Viga” in Mexico City, and other markets located on the Gulf and Caribbean coasts of Mexico. From 134 samples, we obtained sequences from 129, identified to 9 orders, 28 families, 38 genera, and 44 species. The most common species were Seriola dumerili, Pangasianodon hypophthalmus, Carcharhinus falciformis, Carcharhinus brevipinna, and Hypanus americanus. Pangasianodon hypophthalmus was most commonly used as a substitute for higher-value species. The substitution rate was 18% of the total. A review of the conservation status of the specimens identified against the IUNC list enabled us to establish that some species marketed in Mexico are threatened: Makaira nigricans, Lachnolaimus maximus, Hyporthodus flavolimbatus, and Isurus oxyrinchus are classified as vulnerable; Lopholatilus chamaeleonticeps and Sphyrna lewini are endangered; and the status of Hyporthodus nigritus is critical. These results will demonstrate to the Mexican authorities that DNA barcoding is a reliable tool for species identification, even when morphological identification is difficult or impossible.

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