Cloning and sequencing of the rabbit gene encoding T-cell costimulatory molecules

1995 ◽  
Vol 42 (3) ◽  
Author(s):  
Takahiro Isono ◽  
Akira Seto
Keyword(s):  
T Cell ◽  
Costimulatory Molecules ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Rabbit Gene

Partial cloning and sequencing of the gene encoding the porcine T-cell receptor δ-chain constant region

Gene ◽  
1994 ◽  
Vol 144 (2) ◽  
pp. 271-275 ◽  
Author(s):  
D.R. Grimm ◽  
M.L. Misfeldt
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Constant Region ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Δ Chain

scholarly journals Molecular cloning and sequencing of the psaD gene encoding subunit II of photosystem I from the cyanobacterium, Synechocystis sp. PCC 6803.

1988 ◽  
Vol 263 (33) ◽  
pp. 17658-17662
Author(s):  
P Reilly ◽  
J D Hulmes ◽  
Y C Pan ◽  
N Nelson
Keyword(s):  
Molecular Cloning ◽  
Photosystem I ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA).

1986 ◽  
Vol 261 (32) ◽  
pp. 14929-14935
Author(s):  
J W Chase ◽  
B A Rabin ◽  
J B Murphy ◽  
K L Stone ◽  
K R Williams
Keyword(s):  
Escherichia Coli ◽  
Large Subunit ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Exonuclease Vii

scholarly journals The Synechococcus Strain PCC 7942glnN Product (Glutamine Synthetase III) Helps Recovery from Prolonged Nitrogen Chlorosis

2000 ◽  
Vol 182 (19) ◽  
pp. 5615-5619 ◽  
Author(s):  
Jörg Sauer ◽  
Ulrike Dirmeier ◽  
Karl Forchhammer
Keyword(s):  
Glutamine Synthetase ◽  
Transcriptional Start Site ◽  
Binding Motif ◽  
Class Iii ◽  
Wild Type ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Low Concentrations ◽  
Synechococcus Strain ◽  
Pcc 7942

ABSTRACT We report the cloning and sequencing of the glnN gene encoding a class III glutamine synthetase from the cyanobacteriumSynechococcus strain PCC 7942. Mapping of the transcriptional start site revealed a DNA sequence in the promoter region that resembles an imperfect NtcA binding motif. Expression ofglnN is impaired in NtcA- and PII-deficient mutants. The only parameter which was negatively affected in theglnN mutant compared to the wild type was the recovery rate of prolonged nitrogen-starved cells with low concentrations of combined nitrogen.

scholarly journals Identification of potential hot spots in the carboxy-terminal part of the Epstein-Barr virus (EBV) BNLF-1 gene in both malignant and benign EBV-associated diseases: high frequency of a 30-bp deletion in Malaysian and Danish peripheral T-cell lymphomas

Blood ◽  
1994 ◽  
Vol 84 (12) ◽  
pp. 4053-4060 ◽  
Author(s):  
K Sandvej ◽  
SC Peh ◽  
BS Andresen ◽  
G Pallesen
Keyword(s):  
T Cell ◽  
Hot Spots ◽  
Epstein Barr Virus ◽  
Terminal Part ◽  
Gene Encoding ◽  
Single Base ◽  
Barr Virus ◽  
T Cell Lymphomas ◽  
Epstein Barr ◽  
Peripheral T Cell Lymphomas

In this study, we have sequenced the C-terminal part of the Epstein- Barr virus (EBV)-BNLF-1 gene encoding for the latent membrane protein-1 from tissues of EBV-positive Danish Hodgkin's disease (HD) and of Danish and Malaysian peripheral T-cell lymphomas (PTLs) and from tonsils of Danish infectious mononucleosis (IM). Our study showed that some of the 7 single-base mutations and the 30-bp deletion previously detected between codons of amino acid 322 and 366 in the BNLF-1 gene of the nasopharyngeal carcinoma cell line CAO were present in all Malaysian PTLs and in 60% of the Danish PTLs. In HD and the IM cases, the mutations were present in about 30%. The 30-bp deletion and the single base mutations occurred independently, and mutations were detectable in the majority of EBV type B-positive cases. These findings suggest that the 30-bp deletion and the 7 single-base mutations in the C-terminal part of the CAO-BNLF-1 gene do not characterize a new EBV type A substrain. Rather, some of the positions of single base mutations and the 30-bp deletion are hot spots that may have mutated independently through the evolution of EBV strains.

scholarly journals A Novel Gene Encoding Xanthan Lyase of Paenibacillus alginolyticus Strain XL-1

2000 ◽  
Vol 66 (9) ◽  
pp. 3945-3950 ◽  
Author(s):  
Harald J. Ruijssenaars ◽  
Sybe Hartmans ◽  
Jan C. Verdoes
Keyword(s):  
Signal Sequence ◽  
Fold Increase ◽  
Side Chain ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
E Coli ◽  
Mature Enzyme ◽  
Locust Bean ◽  
Encoding Gene ◽  
Amino Acid Signal

ABSTRACT Xanthan-modifying enzymes are powerful tools in studying structure-function relationships of this polysaccharide. One of these modifying enzymes is xanthan lyase, which removes the terminal side chain residue of xanthan. In this paper, the cloning and sequencing of the first xanthan lyase-encoding gene is described, i.e., thexalA gene, encoding pyruvated mannose-specific xanthan lyase of Paenibacillus alginolyticus XL-1. ThexalA gene encoded a 100,823-Da protein, including a 36-amino-acid signal sequence. The 96,887-Da mature enzyme could be expressed functionally in Escherichia coli. Like the native enzyme, the recombinant enzyme showed no activity on depyruvated xanthan. Compared to production by P. alginolyticus, a 30-fold increase in volumetric productivity of soluble xanthan lyase was achieved by heterologous production in E. coli. The recombinant xanthan lyase was used to produce modified xanthan, which showed a dramatic loss of the capacity to form gels with locust bean gum.

Cloning and sequencing of the gene encoding the cell surface glycoprotein of Haloarcula japonica strain TR-1

Extremophiles ◽  
1997 ◽  
Vol 1 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Hiromichi Wakai ◽  
Satoshi Nakamura ◽  
Hiroshi Kawasaki ◽  
Kei Takada ◽  
Shin Mizutani ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Glycoprotein ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Cell Surface Glycoprotein ◽  
Haloarcula Japonica

Cloning and sequencing of a gene from the archaeon Pyrococcus furiosus with high homology to a gene encoding phosphoenolpyruvate synthetase from Escherichia coli

Gene ◽  
1995 ◽  
Vol 160 (1) ◽  
pp. 101-103 ◽  
Author(s):  
Carol E. Jones ◽  
Toni M. Fleming ◽  
Peter W. Piper ◽  
Jennifer A. Littlechild ◽  
Don A. Cowan
Keyword(s):  
Escherichia Coli ◽  
Pyrococcus Furiosus ◽  
High Homology ◽  
Cloning And Sequencing ◽  
Gene Encoding
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