In vitro aging of red blood cells and lipid peroxidation

1987 ◽  
Vol 60 (1-3) ◽  
pp. 163-166 ◽  
Author(s):  
Hermann Einsele ◽  
Michael R. Clemens ◽  
Herbert Remmer
Keyword(s):  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Aging

scholarly journals Detection of short-chain carbonyl products of lipid peroxidation from malaria-parasite (Plasmodium vinckei)-infected red blood cells exposed to oxidative stress

1988 ◽  
Vol 249 (1) ◽  
pp. 63-68 ◽  
Author(s):  
G D Buffinton ◽  
N H Hunt ◽  
W B Cowden ◽  
I A Clark
Keyword(s):  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Malaria Parasite ◽  
Radical Scavenger ◽  
Parasite Plasmodium ◽  
Infected Rbcs ◽  
Carbonyl Products ◽  
Plasmodium Vinckei

Reversed-phase h.p.l.c. was used to detect 2,4-dinitrophenylhydrazine-reactive carbonyl products, which excludes malonaldehyde, in malaria-parasite (Plasmodium vinckei)-infected murine red blood cells (RBCs). A number of alkanals, 4-hydroxyalk-2-enals and alka-2,4-dienals were tentatively identified by comparison with authentic standards. The formation of 4-hydroxynon-2-enal, deca-2,4-dienal and hexanal was greater in P. vinckei-infected RBCs than in their uninfected counterparts and was increased by the presence of t-butyl hydroperoxide. Several of these aldehydes have previously been shown to be toxic to various types of cells, including P. falciparum, in vitro. The iron chelator desferrioxamine and the free-radical scavenger butylated hydroxyanisole inhibited the formation of these aldehydes. These experiments demonstrate that products of lipid peroxidation other than malonaldehyde are formed during the exposure of malaria-infected RBCs in vitro to drugs that generate reactive oxygen species and have anti-parasitic activity. The formation of products of this type during the natural course of malaria infection may have implications for the mechanisms underlying intra-RBC parasite death and the tissue damage associated with the disease.

Oxidative damage to human red blood cells treated with chlorfenvinphos, an organophosphate insecticide (in vitro)

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Bożena Sosnowska ◽  
Bogumiła Huras ◽  
Hanna Nowacka-Krukowska ◽  
Bożena Bukowska
Keyword(s):  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Changes ◽  
Insect Pest ◽  
Human Erythrocytes ◽  
Human Organism ◽  
Organophosphate Insecticide ◽  
High Concentration

AbstractChlorfenvinphos (CFVF) is an organophosphorus insecticide, which was used to control insect pest on livestock and household pests such as flies, fleas, and mites. The molecular basis of toxic properties of CFVF in animals has been insufficiently studied. Blood can transport oxygen and nutrients as well as toxic compounds. Xenobiotics can enter to red blood cells and cause damage. Therefore, investigation of the toxicity of different compounds to erythrocytes is very important. The purpose of the present experiment was to evaluate the effect of this compound on human erythrocytes. We have evaluated the hemolysis, hemoglobin oxidation (met-Hb formation) and lipid peroxidation in human erythrocytes. Moreover, the changes in the level of reactive oxygen species (ROS) were assessed using flow cytometry as well as those in morphological changes of erythrocytes using phase contrast microscopy. This study describes the interaction of low concentrations of CFVF with human erythrocytes as well as the concentrations, which may enter human organism as a result of acute poisoning (0.5–250 μM). It was shown that CFVF only at high concentration induced changes in human erythrocytes. We have observed hemolysis (at 250 μM), changes in morphological parameters including echinocytes formation (at 250 μM), as well as increase in lipid peroxidation in erythrocytes (at 250 μM), ROS formation (at 100 μM) in red blood cells treated 1 hour with CFVF. Additionally, CFVF after 4 h of incubation oxidized hemoglobin, however, to a lower degree.

Interaction of Nitrobenzoates with Haemoglobin in Red Blood Cells and a Haemolysate

1994 ◽  
Vol 13 (5) ◽  
pp. 345-351 ◽  
Author(s):  
E. Norambuena ◽  
L.A. Videla ◽  
E.A. LisSi
Keyword(s):  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Kinetic Data ◽  
Reduction Potential ◽  
Red Cell ◽  
Total Oxidation ◽  
Reactive Material ◽  
Substrate Concentrations

Haemoglobin, either in the intact red blood cells or in their haemolysate, readily reacts with mono- and di-nitrobenzoates. For all the nitroaromatics considered, the rate of the process is faster in the haemolysate than in the whole red blood cell. At low (< 8 mM) concentrations, almost quantitative production of methaemoglobin is observed and the process follows second order kinetics. At higher concentrations, the kinetics become complex and other haemoglobin derivatives are produced. The bimolecular rate constants obtained at low substrate concentrations show little relationship to the nitroaromatic reduction potential. The data indicate that mono-nitrobenzoate derivatives are very active in oxidizing haemoglobin in in vitro erythrocyte suspensions, the activities being similar to that of 3,5-dinitrobenzoate. The measured reactivity follows the order m-nitrobenzoate > 3,5-dinitrobenzoate > p-nitrobenzoate > o-nitrobenzoate and the reactivity of all the compounds is considerably larger than that of nitrobenzene. The present results constitute the first kinetic data bearing on the reactivity of nitroaromatics with haemoglobin, both free and incorporated in the intact red cell. Furthermore, they indicate that the interaction of the nitroaromatics with haemoglobin, leading to total oxidation and transformation, in spite of the total disruption of the membrane, does not produce significant lipid-peroxidation, as measured by chemiluminescence emission, production of TBA reactive material and oxygen consumption.

Melatonin Ameliorates BPA Induced Oxidative Stress in Human Red Blood Cells: An In vitro Study

2020 ◽  
Vol 20 (8) ◽  
pp. 1321-1327
Author(s):  
Saleh M. Abdullah ◽  
Hina Rashid
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Study ◽  
Limited Data ◽  
Human Red Blood Cells ◽  
Living Organisms ◽  
Human Rbcs

Background: Bisphenol A (BPA) is a xenobiotic that causes oxidative stress in various organs in living organisms. Blood cells are also an endpoint where BPA is known to cause oxidative stress. Blood cells, especially red blood cells (RBCs), are crucial for maintaining homeostasis and overall wellbeing of the organism. They are highly susceptible to oxidative stress induced by xenobiotics. However, there is limited data about the oxidative stress induced by BPA in blood, especially in red blood cells. This study was carried out to evaluate BPA induced oxidative stress in human RBCs in vitro and its amelioration by melatonin. Objective: To find if melatonin exerts a protective effect on the oxidative stress induced by the BPA in human red blood cells in vitro. Methods: The erythrocyte suspensions (2 ml) were divided into six groups and treated with 0, 50, 100, 150, 200, and 250 μg/ml of BPA. Another set of erythrocyte suspension with similar BPA treatment and 50 μM Melatonin per group was also set. Incubations lasted for 12 hrs in the dark. Lipid peroxidation, glutathione, glutathione reductase, catalase, and superoxide dismutase were measured as indicators of oxidative stress. Results: BPA caused a significant increase in lipid peroxidation. A decrease in GSH levels was also observed. The activities of all the studied antioxidants also decreased with BPA treatment. Melatonin was seen to mitigate the oxidative stress induced by BPA. Conclusion: Treatment of red blood cells with BPA caused an increase in oxidative stress, while melatonin decreased the induced oxidative stress.

scholarly journals Biochemical Evaluation of the Effects of Hydroxyurea in Vitro on Red Blood Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1599
Author(s):  
Cristiane Oliveira Renó ◽  
Grazielle Aparecida Silva Maia ◽  
Leilismara Sousa Nogueira ◽  
Melina de Barros Pinheiro ◽  
Danyelle Romana Alves Rios ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Low Cost ◽  
Healthy Individuals ◽  
In Vitro Effects ◽  
Almost All

Hydroxyurea (HU) is a low-cost, low-toxicity drug that is often used in diseases, such as sickle cell anemia and different types of cancer. Its effects on the red blood cells (RBC) are still not fully understood. The in vitro effects of HU were evaluated on the biochemical parameters of the RBC from healthy individuals that were treated with 0.6 mM or 0.8 mM HU for 30 min and 1 h. After 30 min, there was a significant increase in almost all of the parameters analyzed in the two concentrations of HU, except for the pyruvate kinase (PK) activity. A treatment with 0.8 mM HU for 1 h resulted in a reduction of the levels of lipid peroxidation, Fe3+, and in the activities of some of the enzymes, such as glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD), and PK. After the incubation for 1 h, the levels of H2O2, lipid peroxidation, reduced glutathione (GSH), enzymatic activity (hexokinase, G6PD, and superoxide dismutase (SOD) were reduced with the treatment of 0.8 mM HU when compared with 0.6 mM. The results have suggested that a treatment with HU at a concentration of 0.8 mM seemed to be more efficient in protecting against the free radicals, as well as in treating diseases, such as sickle cell anemia. HU appears to preferentially stimulate the pentose pathway over the glycolytic pathway. Although this study was carried out with the RBC from healthy individuals, the changes described in this study may help to elucidate the mechanisms of action of HU when administered for therapeutic purposes.

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

Effect of liposomal curcumin on red blood cells in vitro

2013 ◽  
Vol 1 (Suppl. 1) ◽  
pp. A4.1
Author(s):  
Angela Storka
Keyword(s):  
Red Blood Cells ◽  
Blood Cells
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