Hydrogen oxidation in soil following rhizobial H2 production due to N2 fixation by a Vicia faba-Rhizobium leguminosarum symbiosis

1991 ◽  
Vol 11 (3) ◽  
pp. 190-195 ◽  
Author(s):  
S. Schuler ◽  
R. Conrad
Keyword(s):  
Vicia Faba ◽  
N2 Fixation ◽  
Rhizobium Leguminosarum ◽  
Hydrogen Oxidation ◽  
H2 Production

scholarly journals MgtE From Rhizobium leguminosarum Is a Mg2+ Channel Essential for Growth at Low pH and N2 Fixation on Specific Plants

2015 ◽  
Vol 28 (12) ◽  
pp. 1281-1287 ◽  
Author(s):  
Graham Hood ◽  
Ramakrishnan Karunakaran ◽  
J. Allan Downie ◽  
Philip Poole
Keyword(s):  
Pisum Sativum ◽  
Vicia Faba ◽  
N2 Fixation ◽  
Rhizobium Leguminosarum ◽  
Low Ph ◽  
Wild Type ◽  
Vicia Hirsuta ◽  
Native Legume

MgtE is predicted to be a Rhizobium leguminosarum channel and is essential for growth when both Mg2+ is limiting and the pH is low. N2 was only fixed at 8% of the rate of wild type when the crop legume Pisum sativum was inoculated with an mgtE mutant of R. leguminosarum and, although bacteroids were present, they were few in number and not fully developed. R. leguminosarum MgtE was also essential for N2 fixation on the native legume Vicia hirsuta but not when in symbiosis with Vicia faba. The importance of MgtE and the relevance of the contrasting phenotypes is discussed.

Effects of Rhizobium Strain on the Growth, Nodulation, N2 Fixation and Ions Accumulation in Vicia Faba Plant Under Salt Stress

2019 ◽  
Author(s):  
Boulbaba L’taief ◽  
Neila Abdi ◽  
Sihem Smari ◽  
Amel Ayari-Akkari ◽  
Mouna Jeridi ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Vicia Faba ◽  
N2 Fixation ◽  
Rhizobium Leguminosarum ◽  
Solution Culture ◽  
Phosphorus Concentration ◽  
Rhizobium Strain ◽  
Faba Beans ◽  
Stable Growth

Vicia faba L.-rhizobia symbiosis is utilized in different biological ways to improve the productivity of faba beans. This research aims to analyze the effects of the Rhizobium strain on nodulation, N2 fixation, growth, and ion accumulation under salt stress in Viciafaba. The commercial cultivar of faba beans (Viciafaba L. var. minor) was inoculated with the Rhizobium leguminosarumbiovar, by considering viciae strains S10 and S16, after 15 days of growth. This inoculation was carried out in the solution culture consisting of two salt concentrations; 0 mmole l-1NaCl and 50 mmole l-1NaCl. The results revealed that under saline and non-saline conditions, S10 and S16 strains of Rhizobium leguminosarum resulted in the formation of ineffective and effective symbiosis with faba beans. However, the presence of salt stress resulted in increasing the biomass of nodule and nitrogen content. The concentrations of sodium and chloride, in shoot and root, were increased in the presence of salinity. However, potassium concentration was only increased in the shoot. With and without salinity, phosphorus concentration in the shoots was not modified. The results revealed that the salt tolerance of faba beans, inoculated with two strains of Rhizobium were found to possess association with their stable growth. Moreover, the salt tolerance of faba beans inoculated with two salts tolerant rhizobia was also associated with an increment in the capacity of faba beans to increase nodulation and the concentration of shoot N2, Na and Cl-content. In addition, salt tolerance of this variety, inoculated with Rhizobium strains was associated with a decrement in the concentration of K+ in shoot under the salt constraints.

Improved growth and yield of Faba beans (Vicia faba cv. Fiord) by inoculation with strains of Rhizobium leguminosarum biovar. viciae in acid soils in south west Victoria

1994 ◽  
Vol 45 (3) ◽  
pp. 613 ◽  
Author(s):  
JM Carter ◽  
WK Gardner ◽  
AH Gibson
Keyword(s):  
Vicia Faba ◽  
Rhizobium Leguminosarum ◽  
Field Experiments ◽  
Natural Populations ◽  
Acid Soils ◽  
Growth And Yield ◽  
Nitrogen Accumulation ◽  
Commercial Strain ◽  
South West ◽  
Faba Beans

The response of faba beans (Vicia faba L. cv. Fiord) to seed inoculation with eight strains of Rhizobium leguminosarum biovar. viciae was examined in field experiments at six sites on acid soils in south-west Victoria. At two of the sites, two additional strains were examined, and in 1988, 14 strains were examined at one site. Very low natural populations of R. leguminosarum bv. viciae were found at the experimental sites. Most strains resulted in improved early nodulation and increased grain yield at all sites, when compared to inoculation with the commercial strain of rhizobia (SU391). Plant dry matter production and nitrogen accumulation in the plant shoot tissue was also increased at one site during the flowering period by some strains. Large visual differences between plots inoculated with SU391 and other strains were evident at most sites. Most uninoculated treatments were not nodulated and yielded very poorly. Treatments inoculated with the strain SU391 performed similarly to the uninoculated treatments.

Estimating Nitrogenase Activity of Faba Bean (Vicia faba) by Acetylene Reduction (Ar) Assay

1990 ◽  
Vol 17 (5) ◽  
pp. 489 ◽  
Author(s):  
Herdina ◽  
JH Silsbury
Keyword(s):  
Diurnal Variation ◽  
Vicia Faba ◽  
Faba Bean ◽  
Closed System ◽  
N2 Fixation ◽  
Acetylene Reduction ◽  
Nitrogenase Activity ◽  
Electron Flow ◽  
Specific Rate ◽  
Rooting Medium

Methods of conducting acetylene reduction (AR) assay were appraised for estimating the nitrogenase activity of nodules of faba bean (Vicia faba L.). Factors considered were: (i) disturbance of plants when removing the rooting medium; (ii) assay temperature; (iii) the use of whole plants rather than detached, nodulated roots; (iv) diurnal variation in nodule activity; and (v) a decline in C2H4 production after exposure to C2H2. Plants growing in jars of 'oil dry' (calcined clay) had the same AR activity when assayed in situ in a closed system as when assayed after removal of the rooting medium. Assay temperatures of 12.5, 17.5 and 22.5°C influenced the specific rate of AR with the optimum at 17.5°C. Removal of the shoot resulted in a rapid decrease in AR activity in both vegetative and reproductive plants but the effect was much larger in the latter. AR and respiration by nodulated roots were closely linked and both varied markedly over a diurnal 12 h/12 h cycle. Since no fluctuation was found after nodules were detached, diurnal variation in the respiration of nodulated roots is attributed to change in nodule activity. Half of the dark respiration of nodulated roots was associated with respiration of the nodules and thus largely with N2 fixation. Since the AR assay provides no information on how electron flow in vivo is partitioned between reduction of N2 and reduction of protons, diurnal variation in hydrogen evolution (HE) in air and Ar/O2 in an open system was used to estimate this partitioning. Diurnal variation in apparent N2 fixation estimated in this manner was examined at a 'low' PPFD (300 μmol m-2 s-1) and at 'high' (1300 μmol m-2 s-1) to explore whether variation could be attributed to change in carbohydrate supply. Although HE in air and in Ar/O2 were both closely linked with the respiration of the nodulated root, apparent N2 fixation showed only a slight diurnal variation at 'low' light and almost none at 'high'. Vegetative plants showed no C2H2-induced decline in activity with exposure to C2H2 but reproductive plants did. This difference appears to be an age effect rather than attributable to flowering per se, since a decline occurred even when plants were kept vegetative by disbudding. A closed system for AR assay appears satisfactory for vegetative faba bean but such an assay over a 40-min period during the reproductive stage would underestimate nitrogenase activity by about 20%.

​Effects of Microbial Fertilizer and Vermicompost Applications on the Yield and Yield Related Parameters of Broad Bean (Vicia faba L.) under Eastern Mediterranean Highland Agroclimatic Conditions

2021 ◽  
Author(s):  
Özge Uçar
Keyword(s):  
Grain Yield ◽  
Vicia Faba ◽  
Mediterranean Climate ◽  
Rhizobium Leguminosarum ◽  
Broad Bean ◽  
Eastern Mediterranean ◽  
Einkorn Wheat ◽  
Fertile Crescent ◽  
Vicia Faba L ◽  
Location Methods

Background: This study was conducted to determine the effects of microbial fertilizer and vermicompost applications on the yield and yield related parameters of broad beans (Vicia faba L.) under zero chemical fertilizer applied conditions. Trial area was a highland remote to sea under Mediterranean climate in South Eastern Anatolia of Turkey. It is located in fertile crescent and 180 km away to Karacadag Mountains (Diyarbakir) where Einkorn wheat (Aegilops monococcum L.) was first cultivated in history approximately ten thousand years ago. The broad bean was also among the founder crops of the Near East including the trial location. Methods: Rhizobium leguminosarum inoculant were used as microbial fertilizer in the experiments. Applied vermicompost doses were 0, 400, 800 and 1200 kg ha-1. The trials was conducted for two years and replicated thrice in a randomized block deign. Conclusion: Application of vermicompost was found significantly effective on the plant height, first pod height, number of pods per plant, number of seeds per pod, 100 grain weight and grain yield. Aapplication of 800 kg ha-1 and 120 kg ha-1 vermicompost along with Rhizobium leguminosarum inoculation was found superior for grain yield.

Root colonization of faba bean (Vicia faba L.) and pea (Pisum sativum L.) by Rhizobium leguminosarum bv. viciae in the presence of nitrate-nitrogen

2001 ◽  
Vol 47 (12) ◽  
pp. 1068-1074 ◽  
Author(s):  
Chantal J. Beauchamp ◽  
Joseph W. Kloepper ◽  
Joseph J. Shaw ◽  
François-P. Chalifour
Keyword(s):  
Pisum Sativum ◽  
Vicia Faba ◽  
Faba Bean ◽  
Rhizobium Leguminosarum ◽  
Root Colonization ◽  
Nitrate Nitrogen

Growth of Vicia faba as affected by inoculation with vesicular-arbuscular mycorrhizae and Rhizobium leguminosarum bv. viceae in two soils

1994 ◽  
Vol 17 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Y. Z. Ishac ◽  
J. S. Angle ◽  
M. E. El-Borollosy ◽  
M. E. El-Demerdash ◽  
M. I. Mostafa ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Arbuscular Mycorrhizae ◽  
Rhizobium Leguminosarum ◽  
Vesicular Arbuscular Mycorrhizae ◽  
Vesicular Arbuscular

scholarly journals Response of Faba bean (Vicia faba L.) to combined inoculation with Azotobacter and Rhizobium leguminosarum bv. viceae

1999 ◽  
Vol 12 (1) ◽  
pp. 51-59 ◽  
Author(s):  
B Rodelas ◽  
J González-López ◽  
C Pozo ◽  
V Salmerón ◽  
M.V Martı́nez-Toledo
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Rhizobium Leguminosarum ◽  
Vicia Faba L
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