Possibility of passage of epidemic hepatitis virus from the blood serum of patients to leukocyte cultures from healthy donors

1969 ◽  
Vol 68 (3) ◽  
pp. 1010-1013 ◽  
Author(s):  
A. K. Shubladze ◽  
I. F. Barinskii ◽  
A. A. Shatkin ◽  
I. V. Dement'ev ◽  
F. P. Filatov
Keyword(s):  
Blood Serum ◽  
Hepatitis Virus ◽  
Healthy Donors ◽  
Epidemic Hepatitis

scholarly journals Analysis of infrared spectra of blood serum of patients with multiple myeloma

2021 ◽  
Vol 2103 (1) ◽  
pp. 012052
Author(s):  
D A Chernyshev ◽  
E S Mikhailets ◽  
E A Telnaya ◽  
L V Plotnikova ◽  
A D Garifullin ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Principal Component Analysis ◽  
Infrared Spectroscopy ◽  
Blood Serum ◽  
Infrared Spectra ◽  
Early Stage ◽  
Principal Component ◽  
Healthy Donors ◽  
Components Analysis ◽  
Serious Disease

Abstract Multiple myeloma (MM) is a serious disease that is difficult to diagnose especially at early stage. Infrared spectroscopy is a promising approach for diagnosing MM. The principal component analysis (PCA) allows us to reduce the dimension of the data and keep only the important variables. In this study, we apply principal components analysis to infrared (IR) spectra of blood serum from healthy donors and multiple myeloma patients. As a result of the analysis by PCA, it was possible to visualize the separation of patient’s and donor’s samples into two clusters. The result indicates that this method is potentially applicable for diagnosis of multiple myeloma.

scholarly journals Matrix metalloproteinase system in blood serum in patients with squamous cell head and neck carcinomas

2011 ◽  
Vol 10 (1) ◽  
pp. 55-58
Author(s):  
Ye. V. Klisho ◽  
I. V. Kondakova ◽  
Ye. L. Choynzonov ◽  
O. V. Cheremisina ◽  
D. A. Shishkin
Keyword(s):  
Head And Neck Cancer ◽  
Oral Cavity ◽  
Blood Serum ◽  
Head And Neck ◽  
Squamous Cell ◽  
Healthy Subjects ◽  
Head And Neck Carcinoma ◽  
Healthy Donors ◽  
Neck Carcinoma ◽  
Immunoenzymatic Assay

The concentrations of matrix metalloproteinases (MMP-2, -3, -9) and their tissue inhibitors (TIMP-1, -2) were studied in blood serum of 193 patients with squamous cell head and neck carcinoma (SCHNC) and in 38 healthy donors using immunoenzymatic assay. The decreased MMP-3 and TIMP-1, -2 levels were found in patients with oral cavity tumors as compared to patients with laryngeal cancer. The serum MMP-9 and TIMP-1 levels were significantly higher in head and neck cancer patients than in healthy subjects. High TIMP-1 and MMP-9 levels in blood serum of patients with SCHNC should be greatly attended for detection of their predictive and prognostic significances.

scholarly journals The Frequency of Hepatitis Virus Infection Markers Among Highly Qualified Sportsmen

2020 ◽  
Vol 10 (4) ◽  
pp. 305-313
Author(s):  
L. Yu. Ilchenko ◽  
I. A. Morozov ◽  
T. V. Kozhanova ◽  
N. V. Soboleva ◽  
L. I. Melnikova ◽  
...  
Keyword(s):  
Virus Infection ◽  
Blood Serum ◽  
Viral Hepatitis ◽  
Hepatitis A ◽  
Acute Viral Hepatitis ◽  
Hepatitis Virus ◽  
Highly Qualified ◽  
Serum Samples ◽  
Study Results ◽  
Infection Markers

Study Objective is to evaluate prevalence of hepatitis A, B, C, E, and TT virus infection markers in highly qualified sportsmen. Study Design: multicenter open single-site clinical study.Materials and Methods: 100 blood serum samples of sportsmen (game, complex coordination, technical, etc.) were studied. Biological material (blood serum) was obtained from 54 men and 46 women aged 16 to 45 years during an in-depth medical examination. All sportsmen filled out a questionnaire, including demographic data, description of the sport, information about infection risk factors, information about the presence of acute viral hepatitis and vaccination. Anti-HAV IgG, HBsAg, anti-HBcore, anti-HCV, anti-HEV IgG, anti-HEV IgM were determined in the blood serum by enzyme immunoassay; using polymerase chain reaction — DNA Anelloviridae (TTV, TTMDV, TTMV). Study Results: Anti-HAV IgG was detected in 57/66 (86,4%) sportsmen, women (91,2%) predominated, among them a third were engaged in synchronized swimming. 7/57 (12,3%) of the examined had indications of vaccination against hepatitis A. The frequency of anti-HEV IgG did not exceed 3% (2/66). anti-HEV IgM were not detected in any case. Also, none of the examined sportsmen in the blood serum was not determined HBsAg. However, anti-HBcore (marker of latent HBV infection) was detected in 13% (13/100) of the samples. The detection rate of anti-HCV was low, combined with the presence of anti-HBcore was 2% (2/100). In addition, DNA TTV, TTMDV and TTMV, respectively, were found in serum samples from 66/100 (86%), 79/100 (79%), 71/100 of sportsmen. Conclusion: The high frequency of hepatitis virus markers was found (HAV — 74,1%, TTV/TTMDV /TTMV — 71-86%), HBV — 13%, HEV — 3%, HCV — 2%). All patients denied a history of acute viral hepatitis. Vaccination against hepatitis A and B is a modern strategy that prevents infection and the development of acute viral hepatitis. Its mandatory holding should become part of the targeted preparation of sportsmen to achieve the highest sports results. 

Peripheral blood serum from healthy donors contains antibodies against the fragment of transcribed region of ribosomal repeat

2007 ◽  
Vol 144 (3) ◽  
pp. 304-308 ◽  
Author(s):  
N. N. Veiko ◽  
S. V. Kostyuk ◽  
A. V. Ermakov ◽  
E. A. Kalashnikova ◽  
O. A. Kupavtseva ◽  
...  
Keyword(s):  
Blood Serum ◽  
Peripheral Blood ◽  
Healthy Donors ◽  
Ribosomal Repeat

Clinical and prognostic significance of the soluble form of the VISTA immunity control point in patients with primary bone tumors

2021 ◽  
Vol 66 (9) ◽  
pp. 533-538
Author(s):  
N. E. Kushlinskii ◽  
Olga Vladimirovna Kovaleva ◽  
Yu. B. Kuzmin ◽  
E. A. Korotkova ◽  
E. S. Gershtein ◽  
...  
Keyword(s):  
Blood Serum ◽  
Bone Tumors ◽  
Control Point ◽  
Prognostic Significance ◽  
Bone Neoplasms ◽  
Soluble Form ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Malignant Neoplasms ◽  
Healthy Donors

The data of a comparative enzyme-linked immunosorbent assay of the content of the soluble form of the immunity checkpoint VISTA in the blood serum of 30 healthy donors (control group), 79 patients with primary malignant (osteosarcoma - 30, chondrosarcoma - 31, chordoma - 14) and 14 borderline (giant cell tumor) bone neoplasms are presented. In the general group of patients with malignant neoplasms of bones, the median sVISTA content in blood serum is statistically significant lower than in the control (p = 0.040). In patients with bone tumors and healthy donors over 18 years of age, there was a decrease with age in serum sVISTA levels. There were no significant differences in sVISTA concentration between patients with osteosarcoma, chondrosarcoma and healthy donors. Only in patients with chordoma were sVISTA levels statistically significant lower than in controls (p = 0.013). In the groups of patients with chondrosarcoma and osteosarcoma of the bone, there were no significant associations between the serum sVISTA content and the main clinical and morphological characteristics of the disease. In patients with osteosarcoma, no relationship was found between sVISTA levels and overall survival rates, while in patients with bone chondrosarcoma, there was a tendency towards a favorable prognosis with a high content of the marker in the blood serum.

Development of test kit for detection of specific IgM to SARS-CoV-2 by immune blotting in the «Line blot» format

2021 ◽  
Vol 66 (8) ◽  
pp. 472-479
Author(s):  
S. G. Mardanly ◽  
A. S. Avdonina
Keyword(s):  
Blood Serum ◽  
Sensitivity And Specificity ◽  
High Sensitivity ◽  
Confirmatory Test ◽  
Clinical Samples ◽  
Etiological Diagnosis ◽  
Healthy Donors ◽  
Test Kit ◽  
Preliminary Study

Test kit for detection of specific IgM to SARS-CoV-2 by immune blotting in the «Line blot» format has been developed. A preliminary study of diagnostic effectivity on clinical samples of blood serum from patients with COVID-19 and healthy donors showed its high sensitivity and specificity. The new test kit allows to detect IgM to all four structural antigens of SARS-CoV-2 and can be used as a confirmatory test to verify indeterminant screening results in laboratory etiological diagnosis of COVID-19.

scholarly journals Molecular Aggregation in Immune System Activation Studied by Dynamic Light Scattering

Biology ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 123
Author(s):  
Elena Velichko ◽  
Sergey Makarov ◽  
Elina Nepomnyashchaya ◽  
Ge Dong
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Blood Serum ◽  
Immune Complexes ◽  
Circulating Immune Complexes ◽  
Scattering Method ◽  
Dynamic Light Scattering Method ◽  
Immune System Activation ◽  
Healthy Donors ◽  
Biomedical Diagnostics

Determination of the concentration and size of the circulating immune complexes in the blood is an essential part of diagnostics of immune diseases. In this work, we suggest using the dynamic light scattering method to determine the sizes of circulating immune complexes in blood serum. By the dynamic light scattering spectrometer, we found that for healthy and sick donors, the size and concentration of circulating immune complexes differed significantly. The dynamics of formation of these complexes were also examined in this work. It was shown that the formation of immune complexes in the blood of healthy donors is faster than the same reactions in the blood serum of donors with diseases. The results can be used in the diagnostics of the immune status and detection of chronic inflammation. We can recommend the dynamic light scattering method for implementation in biomedical diagnostics.

TRACE ELEMENTS IN BLOOD SERUM OF SÃO PAULO YOUTHS MEASURED BY PIXE

2008 ◽  
Vol 18 (03n04) ◽  
pp. 139-145 ◽  
Author(s):  
S. BERNARDES ◽  
M. H. TABACNIKS ◽  
N. ADDED ◽  
M. A. RIZZUTTO ◽  
M. D. L. BARBOSA ◽  
...  
Keyword(s):  
Trace Elements ◽  
Blood Serum ◽  
Serum Samples ◽  
Concentration Data ◽  
Data Set ◽  
Pixe Analysis ◽  
Elemental Concentrations ◽  
Healthy Donors ◽  
Reference Serum ◽  
Log Normal

The level and change in concentration of trace elements in the fluids of a body may be the result and an evidence of alterations in life functions. In the search for trace element alterations in the human body it is necessary to know referenced values for as many elements as possible. In this work, Proton Induced X-ray Emission (PIXE) was used to study elemental concentrations in human blood serum of 30 healthy donors. The serum samples were obtained by centrifugation and were micro-pipetted on 10µm thick Nuclepore film for PIXE analysis. The elemental concentrations were calculated relative to an internal yttrium standard added during sample preparation. A total of 9 elements were measured (P, S, Cl, K, Ca, Fe, Cu, Zn and Br) in good agreement with literature data. The accuracy of the method was verified analysing reference serum samples from the NIPH-Québec (ICP04S-06 and ICP02S-05). A preliminary statistical analysis indicated a log-normal distribution only for Fe and Cu, while concentration data for the other elements followed the normal distributions. This result indicates the need for stronger statistical data set since the distribution of the elemental concentrations may be a criterion to access their role in biological functions.

scholarly journals Clinical and immunological studies of humoral factors of inflammation and regeneration in patients with injuries of the frontal and basal area

2021 ◽  
pp. 68-71
Author(s):  
Oleksandr D. Bondarchuk ◽  
Oleg F. Melnikov ◽  
Мarina D. Timchenko ◽  
Natalia D. Didyk
Keyword(s):  
Blood Serum ◽  
Transforming Growth Factor ◽  
Basal Area ◽  
Frontal Bone ◽  
Humoral Factors ◽  
Bone Trauma ◽  
Healthy Donors ◽  
Immunological Research ◽  
Γ Interferon ◽  
Anti Inflammation

The authors conducted clinical and immunological research on the identification of сytokine factors of inflammation and regeneration in patients with frontal bone trauma (29) and practically healthy donors (11). Factors of inflammation (interleukin-1β, γ-interferon), anti-inflammation and regeneration (interleukin-34 and transforming growth factor – TGF-1β) were determined in the blood serum by ELISA. It was found that the content of the factor of regeneration decreases and the levels of anti-inflammatory cytokines increase in the blood serum of the patients with frontal traumas with frontal sinus damage.

scholarly journals Peculiarities of inflammatory response during collagenosis

2004 ◽  
Vol 3 (4) ◽  
pp. 21-25
Author(s):  
N. A. Trofimenko ◽  
V. N. Zorina ◽  
S. V. Arkhipova ◽  
Ya. A. Gorbatovsky ◽  
R. M. Zorina
Keyword(s):  
Uric Acid ◽  
Blood Serum ◽  
Lupus Erythematosus ◽  
Albumin Level ◽  
Differential Diagnostics ◽  
Control Group ◽  
Healthy Donors ◽  
Tnf Α ◽  
High Average Level ◽  
Severe Degree

Concentrations of cytokines (IL-1β, IL-6, TNF-α) of α2-macroglobulin (MG), α1-antitrypsin (AT) plasminogen (PL), whole protein, albumin and uric acid in blood serum of patients with collagenosis have been investigated aiming the study of their complex interaction and the possibility of their use during differential diagnostics. The blood serum of 60 healthy donors, 53 patients with rheumatoid arthritis (RA), 15 patients with reactive arthritis (REA) and 16 patients with systemic lupus erythematosus (SLE) has been studied. IL-1β, IL-6 and TNF-α concentrations have been defined by ELISA, MG, AT and PL-rocket immunoelectrophoresis, the whole protein, albumin and uric acid — by biochemical methods. The albumin level decreased in all groups of patients. The whole protein concentration decreased at the first RA activity degree. MG, AT and PL levels had no difference at all diseases as compared to the control group. IL-6 concentration increased significantly at all patients groups. TNF-α increased with the RA severity but differed statistically significantly from REA and SLE only at the most severe degree. Analogous trends in IL-1β concentration have been found in cases of RA and SLE but at REA great individual fluctuations with the high average level have been found. Synchronous change of the studied cytokine concentrations without associated MG level change is evidence of the damage of traffic and regulatory functions of this protein. The uric acid can be used for SLE diagnostics and the dynamic supervision of IL-1β and TNF-α can be a prognostic criterion at RA.

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