Electrical resistance of biological objects to the defibrillator discharge current

1971 ◽  
Vol 71 (6) ◽  
pp. 646-648
Author(s):  
B. M. Tsukerman ◽  
N. A. Len'kova
Keyword(s):  
Discharge Current ◽  
Electrical Resistance ◽  
Biological Objects

scholarly journals DISCREPANCY PARAMETERS OF APPROXIMATIONS OF DISCRETELY SPECIFIED DEPENDENCIES BY ANALYTICAL FUNCTIONS AND SEARCH CRITERIA FOR OPTIMAL VALUES OF THEIR COEFFICIENTS

2021 ◽  
Vol 2021 (59) ◽  
pp. 11-19
Author(s):  
S.M. Zakharchenko ◽  
◽  
N.A. Shydlovska ◽  
I.L. Mazurenko ◽  
◽  
...  
Keyword(s):  
Power Function ◽  
Mathematical Expectation ◽  
Discharge Current ◽  
Electrical Resistance ◽  
Approximation Error ◽  
Analytical Functions ◽  
Spark Erosion ◽  
Instantaneous Values ◽  
Optimal Values

Universal discrepancy parameters of approximations of discretely specified dependencies by analytical functions and search criteria for optimal values of their coefficients, as well as analysis of features of their application are described. Discrepancy parameters of approximations, which do not depend on the ranges of variation of the values of functions and the number of points of a discretely specified dependence, are proposed. They can be effective for objectively comparing the quality of approximations of any dependencies by any functions. Approximations of a discretely specified dependence of the mathematical expectation of the equivalent electrical resistance of a layer of aluminum granules during spark-erosion dispersion in water on the instantaneous values of the discharge current are carried out. As approximating functions, we chose a power function with an exponent factor –1 and a function based on exponential. Using the criteria of the least approximation error, the optimal values of the coefficients of both approximating functions are founded. It is shown in which cases it is advisable to use the combined search criteria for the optimal values of the coefficients of the approximating functions, and in which are enough simple one-component ones. Ref. 27, fig. 2, tables 2.

scholarly journals DISCRETE NONLINEAR-PROBABILISTIC MODEL OF THE EQUIVALENT ELECTRICAL RESISTANCE OF A LAYER OF METAL GRANULES

2021 ◽  
Vol 2021 (2) ◽  
pp. 3-12
Author(s):  
N.A. Shidlovska ◽  
◽  
S.M. Zakharchenko ◽  
Keyword(s):  
Method Of Moments ◽  
Discharge Current ◽  
Probabilistic Model ◽  
Electrical Resistance ◽  
Probabilistic Models ◽  
Tap Water ◽  
Plasma Erosion ◽  
Spark Erosion ◽  
Trailing Edges ◽  
Normal Law

Two strategies for constructing nonlinear-probabilistic models of the equivalent electrical resistance of a layer of metal granules at their spark-erosion and plasma-erosion treatment and algorithms for their implementation are presented. A method for taking into account the parametric properties of such loads in their nonlinear and nonlinear-probabilistic models is described. Based on the data of direct experiments, the distributions of the electrical resistance of a layer of aluminum granules in tap water were obtained for eleven fixed values of the discharge current in it for both the leading and trailing edges of its pulses. The features of these distributions are described for different edges of the discharge current pulses. It has been proved that the obtained distributions can be adequately described by the normal law. The parameters of the normal law for each distribution obtained as a result of direct experiments are founded by the method of moments. A nonlinear-probabilistic model of the equivalent electrical resistance of a layer of aluminum granules in tap water is created, taking into account the main hysteresis of the dependence of resistance on current. It is shown that at large values of discharge currents, the nonlinear-probabilistic model of the equivalent electrical resistance of the layers of metal granules tends to degenerate into a nonlinear model. References 27, figures 4

scholarly journals ANALYTICAL NONLINEAR-PROBABILISTIC MODEL OF THE EQUIVALENT ELECTRICAL RESISTANCE OF A LAYER OF METAL GRANULES

2021 ◽  
Vol 2021 (5) ◽  
pp. 3-10
Author(s):  
S.M. Zakharchenko ◽  
◽  
N.A. Shydlovska ◽  
Keyword(s):  
Mathematical Expectation ◽  
Discharge Current ◽  
Probabilistic Model ◽  
Electrical Resistance ◽  
Nonlinear Models ◽  
Correction Coefficient ◽  
Analytical Functions ◽  
Instantaneous Values ◽  
Optimal Values ◽  
Working Liquid

As a result of processing the experimental data, an analytical continuous nonlinear-probabilistic model of the equivalent electrical resistance of a layer of metal granules in the working liquid was created. It is described by four equations: the modified Gaussian distribution and the dependences on the instantaneous values of the discharge current in the layer of metal granules of the mathematical expectation, dispersion and correction coefficient of the range of its equivalent electrical resistance. Based on the form of the dependences obtained during the experiments and the physics of the processes that occur in this case, two main groups of analytical functions are considered that approximate the obtained dependences. Criteria and methods for finding the optimal values of their coefficients are described. The adequacy of the approximation of each of the three obtained dependences by several analytical functions was investigated, the optimal values of the coefficients of which were found by the described method. Analytical functions was compared, which approximate the dependence of the mathematical expectation of the equivalent electrical resistance of a layer of metal granules on the instantaneous values of the discharge current in it with the known nonlinear models of the resistance of such a medium. References 33, figures 3, tables 3.

High Resolution Microscopy of Multi-Layered Biological Crystals

1982 ◽  
Vol 40 ◽  
pp. 80-83
Author(s):  
H.A. Cohen ◽  
T.W. Jeng ◽  
W. Chiu
Keyword(s):  
High Resolution ◽  
Low Dose ◽  
Three Dimensional ◽  
Data Interpretation ◽  
Two Dimensional ◽  
Biological Objects ◽  
Density Maps ◽  
Density Map ◽  
Complex Crystal ◽  
High Resolution Microscopy

This tutorial will discuss the methodology of low dose electron diffraction and imaging of crystalline biological objects, the problems of data interpretation for two-dimensional projected density maps of glucose embedded protein crystals, the factors to be considered in combining tilt data from three-dimensional crystals, and finally, the prospects of achieving a high resolution three-dimensional density map of a biological crystal. This methodology will be illustrated using two proteins under investigation in our laboratory, the T4 DNA helix destabilizing protein gp32*I and the crotoxin complex crystal.

Studies on Water in the Hydration Chamber of a Modified Electron Microscope

1970 ◽  
Vol 28 ◽  
pp. 544-545
Author(s):  
R. C. Moretz ◽  
G. G. Hausner ◽  
D. F. Parsons
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Electron Microscope ◽  
Steady State ◽  
Room Temperature ◽  
Small Mass ◽  
Equilibrium Pressure ◽  
Biological Objects ◽  
Mechanical Pump ◽  
Differential Pumping

Use of the electron microscope to examine wet objects is possible due to the small mass thickness of the equilibrium pressure of water vapor at room temperature. Previous attempts to examine hydrated biological objects and water itself used a chamber consisting of two small apertures sealed by two thin films. Extensive work in our laboratory showed that such films have an 80% failure rate when wet. Using the principle of differential pumping of the microscope column, we can use open apertures in place of thin film windows.Fig. 1 shows the modified Siemens la specimen chamber with the connections to the water supply and the auxiliary pumping station. A mechanical pump is connected to the vapor supply via a 100μ aperture to maintain steady-state conditions.

Improved 3-D reconstruction using stereo computer graphics and multiple tilt EM images

1995 ◽  
Vol 53 ◽  
pp. 630-631
Author(s):  
Lee D. Peachey ◽  
Lou Fodor ◽  
John C. Haselgrove ◽  
Stanley M. Dunn ◽  
Junqing Huang
Keyword(s):  
Computer Graphics ◽  
High Performance ◽  
Transverse Section ◽  
Three Dimensional ◽  
Stereo Pair ◽  
3D Reconstructions ◽  
Video Cameras ◽  
Biological Objects ◽  
Microscope Images ◽  
High Performance Computer

Stereo pairs of electron microscope images provide valuable visual impressions of the three-dimensional nature of specimens, including biological objects. Beyond this one seeks quantitatively accurate models and measurements of the three dimensional positions and sizes of structures in the specimen. In our laboratory, we have sought to combine high resolution video cameras with high performance computer graphics systems to improve both the ease of building 3D reconstructions and the accuracy of 3D measurements, by using multiple tilt images of the same specimen tilted over a wider range of angles than can be viewed stereoscopically. Ultimately we also wish to automate the reconstruction and measurement process, and have initiated work in that direction.Figure 1 is a stereo pair of 400 kV images from a 1 micrometer thick transverse section of frog skeletal muscle stained with the Golgi stain. This stain selectively increases the density of the transverse tubular network in these muscle cells, and it is this network that we reconstruct in this example.

X-ray Gabor holography

1995 ◽  
Vol 53 ◽  
pp. 612-613
Author(s):  
Steve Lindaas ◽  
Chris Jacobsen ◽  
Alex Kalinovsky ◽  
Malcolm Howells
Keyword(s):  
Surface Relief ◽  
Biological Imaging ◽  
Specimen Preparation ◽  
X Rays ◽  
X Ray ◽  
Water Window ◽  
Biological Objects ◽  
Transmission Imaging ◽  
Atomic Force ◽  
Electron Microscopes

Soft x-ray microscopy offers an approach to transmission imaging of wet, micron-thick biological objects at a resolution superior to that of optical microscopes and with less specimen preparation/manipulation than electron microscopes. Gabor holography has unique characteristics which make it particularly well suited for certain investigations: it requires no prefocussing, it is compatible with flash x-ray sources, and it is able to use the whole footprint of multimode sources. Our method serves to refine this technique in anticipation of the development of suitable flash sources (such as x-ray lasers) and to develop cryo capabilities with which to reduce specimen damage. Our primary emphasis has been on biological imaging so we use x-rays in the water window (between the Oxygen-K and Carbon-K absorption edges) with which we record holograms in vacuum or in air.The hologram is recorded on a high resolution recording medium; our work employs the photoresist poly(methylmethacrylate) (PMMA). Following resist “development” (solvent etching), a surface relief pattern is produced which an atomic force microscope is aptly suited to image.

Prospects for scanned-probe microscopy

1994 ◽  
Vol 52 ◽  
pp. 6-7
Author(s):  
Jean-Paul Revel
Keyword(s):  
High Vacuum ◽  
Physiological Saline ◽  
Scanning Probe ◽  
Aqueous Environment ◽  
Whole Cells ◽  
Material Scientist ◽  
Biological Objects ◽  
Spatially Resolved ◽  
Atomic Force ◽  
Scanned Probe Microscopy

In the last 50+ years the electron microscope and allied instruments have led the way as means to acquire spatially resolved information about very small objects. For the material scientist and the biologist both, imaging using the information derived from the interaction of electrons with the objects of their concern, has had limitations. Material scientists have been handicapped by the fact that their samples are often too thick for penetration without using million volt instruments. Biologists have been handicapped both by the problem of contrast since most biological objects are composed of elements of low Z, and also by the requirement that sample be placed in high vacuum. Cells consist of 90% water, so elaborate precautions have to be taken to remove the water without losing the structure altogether. We are now poised to make another leap forwards because of the development of scanned probe microscopies, particularly the Atomic Force Microscope (AFM). The scanning probe instruments permit resolutions that electron microscopists still work very hard to achieve, if they have reached it yet. Probably the most interesting feature of the AFM technology, for the biologist in any case, is that it has opened the dream of high resolution in an aqueous environment. There are few restrictions on where the instrument can be used. AFMs can be made to work in high vacuum, allowing the material scientist to avoid contamination. The biologist can be made happy as well. The tips used for detection are made of silicon nitride,(Si3N4), and are essentially unaffected by exposure to physiological saline (about which more below). So here is an instrument which can look at living whole cells and at atoms as well.

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