Three-dimensional cell cycle model with distributed transcription and translation

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

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Three-dimensional cell-adhesive matrix of silk cocoon derived carbon fiber assembled with iron-porphyrin for monitoring cell released signal molecules

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2021.129594 ◽

2021 ◽

Vol 334 ◽

pp. 129594

Author(s):

Fang Xin Hu ◽

Xiaoli Xie ◽

Dongping Wang ◽

Hong Bin Yang ◽

Yu Gu ◽

...

Keyword(s):

Carbon Fiber ◽

Three Dimensional ◽

Iron Porphyrin ◽

Signal Molecules ◽

Silk Cocoon ◽

Adhesive Matrix ◽

Cell Adhesive ◽

Dimensional Cell

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Propagation‐based Phase Contrast Computed Tomography as a Suitable Tool for the Characterization of Spatial Three‐Dimensional Cell Distribution in Biomaterials

Advanced Engineering Materials ◽

10.1002/adem.202001188 ◽

2021 ◽

Author(s):

D.C. Florian Wieland ◽

Simone Krueger ◽

Julian Moosmann ◽

Thomas Distler ◽

Alina Weizel ◽

...

Keyword(s):

Computed Tomography ◽

Phase Contrast ◽

Three Dimensional ◽

Cell Distribution ◽

Suitable Tool ◽

Dimensional Cell

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Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

In Vitro Cellular & Developmental Biology - Animal ◽

10.1007/s11626-020-00537-3 ◽

2021 ◽

Author(s):

Terry Riss ◽

O. Joseph Trask

Keyword(s):

Cell Culture ◽

Three Dimensional ◽

3D Culture ◽

Fluorescent Imaging ◽

Culture Models ◽

Assay Methods ◽

Diffusion Rates ◽

Cell Culture Models ◽

Dimensional Cell ◽

Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

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