Effect of dietary fats on some membrane-bound enzyme activities, membrane lipid composition and fatty acid profiles of rat heart sarcolemma

Lipids ◽  
1992 ◽  
Vol 27 (5) ◽  
pp. 339-343 ◽  
Author(s):  
A. Vajreswari ◽  
K. Narayanareddy
Keyword(s):  
Fatty Acid ◽  
Lipid Composition ◽  
Enzyme Activities ◽  
Rat Heart ◽  
Membrane Lipid ◽  
Dietary Fats ◽  
Fatty Acid Profiles ◽  
Membrane Lipid Composition ◽  
Heart Sarcolemma ◽  
Membrane Bound

scholarly journals Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases

2014 ◽  
Vol 46 (4) ◽  
pp. 273-282 ◽  
Author(s):  
M. S. Jaureguiberry ◽  
M. A. Tricerri ◽  
S. A. Sanchez ◽  
G. S. Finarelli ◽  
M. A. Montanaro ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Fatty Acid ◽  
Cell Line ◽  
Lipid Composition ◽  
Membrane Lipid ◽  
Fatty Acid Desaturases ◽  
Cellular Homeostasis ◽  
Membrane Lipid Composition ◽  
Plasma Membrane Lipid

Effects of temperature on the structure and metabolism of cell membranes in fish

1984 ◽  
Vol 246 (4) ◽  
pp. R460-R470 ◽  
Author(s):  
J. R. Hazel
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Cold Exposure ◽  
Lipid Composition ◽  
Membrane Lipid ◽  
Effect Of Temperature ◽  
Salmo Gairdneri ◽  
Homeoviscous Adaptation ◽  
Cold Temperatures ◽  
Membrane Lipid Composition

The metabolic adjustments responsible for the “homeoviscous adaptation” of membrane lipid composition in fish are examined with special reference to the rainbow trout, Salmo gairdneri. The percentage of fatty acid lipogenesis attributable to unsaturates was elevated after an acute drop in temperature but declined with continued cold exposure (i.e., cold acclimation). In contrast, selected desaturation reactions [particularly those involved in the production of polyunsaturated fatty acids (PUFA) of the n-3 and/or n-6 families] proceeded more rapidly in cold-than in warm-acclimated trout. Different time courses for the change in monoene and PUFA levels of hepatic microsomal membranes during thermal acclimation suggest that the various desaturase enzymes contribute to the acclimatory response at different times. Certain fatty acids, particularly the delta 5-desaturation products of the n-3 (20:5 delta 5,8,11,14,17) and n-6 (20:4 delta 5,8,11,14) series, were preferentially incorporated into phospholipids at cold temperatures and by cold-acclimated trout, due in part to the direct effect of temperature on the substrate preferences of the phospho- and acyltransferase enzymes of de novo phospholipid biosynthesis; however, chain length rather than degree of unsaturation per se may determine the temperature-dependent pattern of fatty acid incorporation. Both acute and chronic cold exposure elevated the incorporation of PUFA into phosphatidylserine (PS), suggesting that the conversion of PS to phosphatidylethanolamine (PE) may be activated at cold temperatures. The rate of homeoviscous adaptation appears to be limited by the rate of membrane lipid turnover, which although generally positively correlated with acclimation temperature, did vary depending on the phospholipid moiety and tissue considered. Finally the direct acylation of lysophospholipids formed during the process of membrane turnover may contribute to both rapid and acclimatory adjustments in membrane lipid composition.

Effects of an olive-oil-rich diet on erythrocyte membrane lipid composition and cation transport systems

1989 ◽  
Vol 76 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Antonio Pagnan ◽  
Roberto Corrocher ◽  
Giovanni B. Ambrosio ◽  
Silvana Ferrari ◽  
Patrizia Guarini ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Olive Oil ◽  
Saturated Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Lipid Composition ◽  
Cation Transport ◽  
Membrane Lipid ◽  
Membrane Lipid Composition ◽  
Acid Ratio ◽  
Saturated Fatty Acid Ratio

1. In this study we sought to test the possibility that an olive-oil-rich diet might influence the membrane lipid composition and cation transport mechanisms in erythrocytes. 2. To this end, 11 normotensive normolipidaemic healthy volunteers were given a standard relatively low fat diet (28% of total energy) for 3 weeks, followed by a high fat (38% of total energy), olive-oil (100 g/day)-enriched diet for the 3 subsequent weeks, in a metabolic ward. The two diets contained the same amount of cholesterol (400 mg), and the polyunsaturated fatty acid/saturated fatty acid ratio was similar and comparatively low (0.35 on the standard vs 0.36 on the olive oil diet). 3. At the end of the study the lipid composition of the erythrocyte membranes showed the following changes: the oleic acid content increased by 15.7% and the amount of saturated fatty acid decreased by 3.2%. The polyunsaturated fatty acid content tended to decrease, while the polyunsaturated fatty acid/saturated fatty acid ratio did not change significantly. The cholesterol/phospholipid molar ratio increased significantly by 7.8%, still remaining within the reference range. 4. At the same time, the mean values of the maximum rates of the cation fluxes mediated by the Na+-K+ pump, and by Na+-K+ co-transport rose significantly, while the maximal rate of Na+-Li+ countertransport showed a non-significant trend towards lower values. 5. Urinary excretion of Na+ and K+, body weight and blood pressure did not change significantly.

Regulation of allosteric membrane-bound enzymes through changes in membrane lipid composition

1975 ◽  
Vol 415 (2) ◽  
pp. 231-251 ◽  
Author(s):  
Ricardo N. Farías ◽  
Bernabé Bloj ◽  
Roberto D. Morero ◽  
Faustino Siñeriz ◽  
Raúl E. Trucco
Keyword(s):  
Lipid Composition ◽  
Membrane Lipid ◽  
Membrane Lipid Composition ◽  
Membrane Bound ◽  
Membrane Bound Enzymes

scholarly journals Dietary α-linolenic acid supplementation alters skeletal muscle plasma membrane lipid composition, sarcolemmal FAT/CD36 abundance, and palmitate transport rates

2016 ◽  
Vol 311 (6) ◽  
pp. R1234-R1242 ◽  
Author(s):  
Zane Chorner ◽  
Pierre-Andre Barbeau ◽  
Laura Castellani ◽  
David C. Wright ◽  
Adrian Chabowski ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acids ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Linolenic Acid ◽  
Lipid Composition ◽  
Membrane Lipid ◽  
Membrane Lipid Composition ◽  
Plasma Membrane Lipid

The cellular processes influenced by consuming polyunsaturated fatty acids remains poorly defined. Within skeletal muscle, a rate-limiting step in fatty acid oxidation is the movement of lipids across the sarcolemmal membrane, and therefore, we aimed to determine the effects of consuming flaxseed oil high in α-linolenic acid (ALA), on plasma membrane lipid composition and the capacity to transport palmitate. Rats fed a diet supplemented with ALA (10%) displayed marked increases in omega-3 polyunsaturated fatty acids (PUFAs) within whole muscle and sarcolemmal membranes (approximately five-fold), at the apparent expense of arachidonic acid (−50%). These changes coincided with increased sarcolemmal palmitate transport rates (+20%), plasma membrane fatty acid translocase (FAT/CD36; +20%) abundance, skeletal muscle triacylglycerol content (approximately twofold), and rates of whole body fat oxidation (~50%). The redistribution of FAT/CD36 to the plasma membrane could not be explained by increased phosphorylation of signaling pathways implicated in regulating FAT/CD36 trafficking events (i.e., phosphorylation of ERK1/2, CaMKII, AMPK, and Akt), suggesting the increased n-3 PUFA composition of the plasma membrane influenced FAT/CD36 accumulation. Altogether, the present data provide evidence that a diet supplemented with ALA increases the transport of lipids into resting skeletal muscle in conjunction with increased sarcolemmal n-3 PUFA and FAT/CD36 contents.

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