Cardioprotective response to chronic administration of vitamin E in isoproterenol induced myocardial necrosis: Hemodynamic, biochemical and ultrastructural studies

The immunosuppressant drug cyclosporin A (CyA) has been used in diseases with immunological basis and in transplant patients. Nephrotoxicity and hepatotoxicity are the main adverse effects of this drug. To find a protective drug against those effects we assayed the cardioprotector Trimetazidine (TMZ) and vitamin E, used as nutritional supplements to alleviate oxidative stress. Six groups of eight male Wistar rats each were prepared (groups A–F): A, control; B, vitamin E (10 mg/Kg/day); C, TMZ (20 mg/Kg/day); D, 25 mg/Kg/day CyA; E, CyA and vitamin E (25 mg/Kg/day CyA + 10 mg/Kg/day Vit E); F, TMZ for 20 days (20 mg/kg/day); and then CyA (25 mg/kg/day) and TMZ (20 mg/Kg/day). The experiment lasted 120 days. The exposure of rats to CyA promoted nephrotoxicity and hepatotoxicity with an increase in serum urea, creatinine, and glutamate dehydrogenase (GLDH). Structural and ultrastructural studies of liver and kidney were performed. Group D showed adverse effects induced by CyA since statistically significant differences were found with respect to the control group (A). Vitamin E (E) showed no protective effect. Pretreatment with TMZ (F) attenuated the adverse effects of CyA. We conclude that CyA-induced nephrotoxicity and hepatotoxicity are attenuated by the cytoprotective effect of TMZ. TMZ inhibits the reabsorption and, consequently, the accumulation of CyA in the cell. The antioxidant capacity of vitamin E did not improve the effect of CyA.

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Cardioprotective effect of lemon grass as evidenced by biochemical and histopathological changes in experimentally induced cardiotoxicity

Human & Experimental Toxicology ◽

10.1177/0960327110386391 ◽

2010 ◽

Vol 30 (8) ◽

pp. 1073-1082 ◽

Cited By ~ 9

Author(s):

K. Gayathri ◽

KS Jayachandran ◽

Hannah R Vasanthi ◽

G. Victor Rajamanickam

Keyword(s):

Vitamin E ◽

Myocardial Necrosis ◽

Heart Tissue ◽

Cardioprotective Effect ◽

Albino Rats ◽

Enzymatic Antioxidants ◽

Group V ◽

Lemon Grass ◽

Heart Homogenate ◽

Wistar Albino Rats

Isoproterenol is a synthetic catecholamine found to cause toxicity leading to severe stress in the myocardium of experimental animals. The aim of the present study is to evaluate the cardioprotective effect of Cymbopogon citratus, which is used as a culinary item and commonly known as lemon grass (LG), in isoproterenol-induced cardiotoxicity. Male Wistar albino rats were segregated into five different groups as follows. Groups I and II rats were treated with vehicle. Groups III and IV rats were treated with 100 and 200 mg/kg b.wt. of LG. Group V with 100 mg/kg b.wt. of vitamin E. Myocardial necrosis was induced in Groups II, III, IV and V on 58th and 59th day using isoproterenol at a dose of 85 mg/kg twice at 24-hour interval. Animals were sacrificed on the 60 th day. LG pretreatment exhibited cardioprotective activity as evidenced by decreased activity of cardiac markers in serum and increased the same in heart homogenate (p < 0.05). LG administration decreased the toxic events of lipid peroxidation (TBARS) in both serum and heart tissue, by increasing the level of enzymatic antioxidants and non-enzymatic antioxidants significantly in both heart homogenate and serum sample (p < 0.05). The histopathological observations also revealed that the cardioprotective effect of LG extract was observed at a dose of 200 mg/kg b.wt. The results of the present study reveal that LG is cardioprotective and antilipid peroxidative by increasing various antioxidants at a dose of 200 mg/kg b.wt., which is comparable with that of vitamin E.

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Chronic administration of vitamin E elevates brain derived neurotrophic factor and nerve growth factor in chronic stress induced wistar rats.

International Journal of Pharma and Bio Sciences ◽

10.22376/ijpbs.2017.8.3.b1007-1016 ◽

2017 ◽

Vol 8 (3) ◽

Author(s):

SANGEETHA A ◽

KUMARESAN M ◽

VIJAYARAGHAVAN RAJAGOPALAN ◽

SENTHILKUMAR SIVANESAN ◽

SURAPANENI KRISHNA MOHAN

Keyword(s):

Vitamin E ◽

Nerve Growth Factor ◽

Growth Factor ◽

Chronic Stress ◽

Neurotrophic Factor ◽

Wistar Rats ◽

Chronic Administration ◽

Brain Derived Neurotrophic Factor ◽

Nerve Growth

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Paraoxon and parathion: effects of daily administration on vitamin E levels in male rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y70-056 ◽

1970 ◽

Vol 48 (6) ◽

pp. 342-349 ◽

Cited By ~ 4

Author(s):

G. R. Goyer ◽

E. A. Martin ◽

P. Paganuzzi ◽

J. Brodeur

Keyword(s):

Vitamin E ◽

Plasma Concentrations ◽

Chronic Administration ◽

Liver Weight ◽

Acetylcholinesterase Activity ◽

The Body ◽

Daily Administration ◽

Male Rats ◽

Male Rat ◽

Cholinesterase Inhibition

The effect of daily administration of parathion and paraoxon on hepatic stores and plasma concentrations of vitamin E was studied in the immature male rat. Parathion or paraoxon (30 p.p.m.) was added to the daily diet of various groups of eight immature animals (70 to 90 g). Control groups received a basic rat diet. At intervals of 2, 4, and 8 weeks after the beginning of treatment, control and treated animals were killed. A significant decrease in the body weight of the treated animals was noticed during the first 2 weeks of treatment. Food intake, liver weight, and plasma and liver tocopherol concentrations were not modified throughout the experiments in treated animals by comparison with controls. A marked inhibition of brain acetylcholinesterase activity (81%) was observed after 1 week of treatment with either parathion or paraoxon. During the following weeks, a slight recovery of acetylcholinesterase activity was observed in the paraoxon-treated groups: indeed, the cholinesterase inhibition decreased from 81% to 67% after 8 weeks. With parathion-treated animals, the recovery was more pronounced as the cholinesterase inhibition decreased from 81% to 33% after 8 weeks. Additional experiments revealed that the metabolism of paraoxon or parathion by liver homogenates was not stimulated in chronically treated rats. Thus, chronic administration of paraoxon or parathion does not seem to interfere with liver and plasma levels of vitamin E.

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Chronic administration of vitamin E in type 2 diabetic Nigerians

Abia State University Medical Students Association Journal ◽

10.4314/asumsaj.v4i1.42558 ◽

2009 ◽

Vol 4 (1) ◽

Author(s):

IN Nweke ◽

S Chinenye ◽

AC Ojule

Keyword(s):

Vitamin E ◽

Chronic Administration ◽

Type 2 Diabetic

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Vitamin E improved redox homeostasis in heart and aorta of hypothyroid rats

Endocrine Regulations ◽

10.1515/enr-2017-0021 ◽

2017 ◽

Vol 51 (4) ◽

pp. 205-212 ◽

Cited By ~ 1

Author(s):

M. Hedayati ◽

S. Niazmand ◽

M. Hosseini ◽

Y. Baghcheghi ◽

F. Beheshti ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Tap Water ◽

Redox Homeostasis ◽

Chronic Administration ◽

Control Group ◽

Cardiac Tissues ◽

Water Group ◽

Total Thiol ◽

Antioxidant Defense Enzymes

Abstract Objectives. The objective of this study was to evaluate the effect of vitamin E on the oxidative stress parameters and antioxidant defense enzymes in the heart and aorta of 6-n-propylthiouracil (PTU)-induced hypothyroid rats. Methods. The animals were divided into 4 experimental groups: Group 1 (Euthyroid) received tap water, Group 2 (Hypothyroid) received 0.05 % of PTU in dissolved in their drinking water, Group 3 (PTU+Vit E) hypothyroid rats treated with vitamin E, and Group 4 (Euthyroid+Vit E). Vitamin E was injected daily (20 mg/kg) to groups 3 and 4 via daily gavage for 6 weeks. Malondialdehyde (MDA) levels, total thiol levels, and the activities of Cu, Zn-superoxide dismutase (SOD) and catalase (CAT) were evaluated in the aortic and cardiac tissues. Results. A significant decrease of thyroxine (T4) serum levels confirmed hypothyroidism in rats, which received PTU. The MDA level increased and total thiol level decreased in the hypothyroid group compared to control group (p<0.001). Th e activities of SOD and CAT significantly decreased in the hypothyroid rats in comparison to the control. Vitamin E treatment resulted in increased levels of total thiol, SOD, and CAT within aortic and cardiac tissues and decreased levels of MDA in comparison with the hypothyroid group (p<0.01−p<0.001). Conclusions. PTU-induced hypothyroidism resulted in oxidative stress. Chronic administration of vitamin E to hypothyroid rats decreased the oxidative stress markers in the aortic and cardiac tissues.

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