Construction of a Gene Bank of Azorhizobium IRBG-46: Isolation of hup Genes

1993 ◽  
Vol 2 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Anjali Mehta ◽  
S. P. S. Khanuja ◽  
M. L. Lodha
Keyword(s):  

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 513
Author(s):  
Antonín Dreiseitl

Gene bank accessions are necessary for implementing many research and breeding projects. However, a great number of accessions are contaminated or confused. If such accessions are used, the results obtained from these projects are inaccurate and non-reproducible. There are methods that allow almost perfect genotype identification; nevertheless, they are relatively recent and results cannot be compared with the characteristics of the original accessions. Growing resistant cultivars is an environmentally safe and cheap way of disease management and knowledge of diverse resistance genes and their combinations can be used to identify varieties and verify their authenticity and homogeneity. For this purpose, all 172 accessions of the core collection (CC) of the Czech winter barley (Hordeum vulgare) gene bank, originating from 35 countries, were studied. For resistance tests, 51 reference isolates of Blumeria graminis f. sp. Hordei, collected in all nonpolar continents over a period of 63 years and representing the global virulence/avirulence diversity of the pathogen, were used. Only 25 barley accessions were homogeneous (genetically uniform), whereas 147 accessions were heterogeneous due to presence of different genotypes. In total, 17 resistance genes were found singly or in combinations; 76.3% of accessions with identified resistance genes carried alleles at the Mla locus. To purify the CC, progenies of individual plants must be multiplied and authenticity and homogeneity of the seed should be confirmed with resistance tests, and subsequently can be studied with more advanced methods.



Agricultura ◽  
2016 ◽  
Vol 13 (1-2) ◽  
pp. 57-64 ◽  
Author(s):  
Metka Šiško

Abstract The main goal of the Slovene Plant Gene Bank is preservation, maintenance and evaluation of traditional cultivars and other useful genotypes. The Faculty of Agriculture and Life Sciences houses among other plant materials also numerous accessions of plums (Prunus domestica L.). Duplicates among 15 accessions were studied using six microsatellite primer pairs. These microsatellite markers revealed an average of 7.67 alleles per locus, and a range of 4 to 10 different alleles per locus. The genetic distances between studied accessions were calculated using the Dice coefficient to form a dendrogram. The six SSRs were found to be adequate for differentiating among genotypes within the collection. Among the analysed accessions no duplicates were found.



1989 ◽  
Vol 35 (4) ◽  
pp. 487-491 ◽  
Author(s):  
Paul H. Goodwin

Xylella fastidiosa DNA, partially digested with Sau3A, was ligated into the cosmid vector, pUCD615. Approximately 4500 ampicillin-resistant Escherichia coli colonies were obtained. The frequency of complementation of leucine auxotrophy in transfected E. coli indicated that the cosmid gene bank was representative of X. fastidiosa genomic DNA. Colonies were lysed directly onto nitrocellulose membranes using a thermo-inducible λ lysogen and screened for expression of X. fastidiosa antigens. Approximately 16.5% of a random sample of clones were found to express X. fastidiosa antigens as determined by Western blots. These proteins comigrated with proteins of X. fastidiosa and ranged in molecular weight from 10 000 to 160 000. Conjugation of several of the plasmids into Erwinia stewartii resulted in expression of the similar molecular weight cloned proteins with similar levels of expression as in E. coli.Key words: Xylella fastidiosa, Pierce's disease, immunological clone screening, thermo-inducible lysogeny.





Author(s):  
P. Benedek ◽  
T. Szabó ◽  
M. Soltész ◽  
Z. Szabó ◽  
C. Konrád-Németh

We evaluated 285 pear genotypes (commercial cultivars, ancient local varieties, unnamed local strains, seedlings, wild seedlings) in the largest gene bank of pear in Hungary from the point of view of psylla resistance to explore their possible exploitation in organic farming. We have found some 10 new resistant types (Bókoló körte, Bôtermô Kálmán, Füge alakú körte, Nagyasszony körte, Nyári Kálmán, Rozs nyári körte, Viki körte, Pb-242, Pb-299, 0-632) and 7 highly tolerant ones (Cure-6, Kései Kálmán, Kieffer, Kieffer Éd, Steiner, Téli Kálmán, II. B-3- 6/4, 96-16/5) (Table 1). These made up 3.5 + 2.8 per cent of the investigated genotypes, while 93.7 per cent of them were susceptible to pear psylla damage. Taking earlier and present results into account we can list more than 30 European pear cultivars being resistant or highly tolerant to pear psylla infestation and damage. In fact, the list of resistant and highly tolerant cultivars may serve as a basis selecting pear cultivars fitting to the specific requirements of the organic farming. By the end we can conclude that there is some real hope to exploit some resistant or highly tolerant ancient or local cultivars in organic farming but further investigations are needed to estimate their yield capacity and fruit quality.



Genetika ◽  
2010 ◽  
Vol 42 (1) ◽  
pp. 9-21 ◽  
Author(s):  
Jelena Vancetovic ◽  
Snezana Mladenovic-Drinic ◽  
Milosav Babic ◽  
Dragana Ignjatovic-Micic ◽  
Violeta Andjelkovic

Characterization and evaluation of the genetic resources provide breeders with valuable information on an effective utilization of the genetic resources in breeding programs. In this paper we present the results of different research programs aimed at identification of superior genotypes among MRI gene bank accessions, regarding stress tolerance (drought and herbicides), better nutritional quality (phosphorus) and specific traits (cytoplasmic male sterility - CMS). Fifty-two genotypes were identified as a potential source for drought tolerance. Considering herbicide tolerance only genotypes with resistance to the Pivot were found. Within 100 sources of CMS in the collection S cytoplasm was identified as the predominant type. Phytate analysis of 60 maize populations identified three groups of populations - with low (8), intermediate (25) and high (27) phytate content. The results of these researches, which are a part of pre-breeding activities, will be included in MRI breeding programs, with the aim of developing new genotypes with improved traits important in commercial maize breeding and seed production.



Genetika ◽  
2013 ◽  
Vol 45 (1) ◽  
pp. 145-151
Author(s):  
Jelena Vancetovic ◽  
Dragana Ignjatovic-Micic ◽  
Ana Nikolic ◽  
Sofija Bozinovic ◽  
Ksenija Markovic ◽  
...  

In gene-bank maize collection of Maize Research Institute Zemun Polje (MRI) two samples with untypical mtDNA profile for cytoplasmic male sterility (cms) were identified. These two samples showed typical multiplex polymerase chain reaction (PCR) band for cms-S, but also an additional band of unknown nature. It is assumed that the additional band is the result of a rearrangement of the two mitochondrial episomes characteristic for the cms-S in maize or a duplication of the part of cms-S mitochondrial genome. Additional field and laboratory experiments are necessary in the further lightening of this phenomenon.



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