scholarly journals Bioactivites of Penicillium citrinum isolated from a medicinal plant Swertia chirayita

2021 ◽  
Author(s):  
Hemant Sharma ◽  
Arun Kumar Rai ◽  
Rajen Chettri ◽  
Poonam Singh Nigam
Keyword(s):  
Medicinal Plant ◽  
Fusarium Solani ◽  
Colletotrichum Gloeosporioides ◽  
Indoleacetic Acid ◽  
Fungal Endophytes ◽  
Penicillium Citrinum ◽  
Project Work ◽  
Swertia Chirayita ◽  
Industrial Sectors ◽  
Molecular Studies

AbstractEndophytes associated with plants have the property to produce active biomolecules with their possible applications in agro-industrial sectors. This study provides a project work on analyzing various activities of fungal endophytes isolated from Swertia chirayita of Sikkim Himalayan region. Among several fungal endophytes screened, isolate UTCRF6 was found most active with the secretion of enzymes protease, cellulase, amylase and chitinase, as well as other metabolites Indoleacetic acid and siderophores. This endophyte was found active in restricting the growth of phyto-pathogens, including strains of Fusarium solani, Colletotrichum gloeosporioides, Alternaria alternata, Pestalotiopsis theae and Sclerotinia sclerotiorum. Morphological and molecular studies of this endophytic fungus showed similarity with Penicillium citrinum. Graphic abstract

scholarly journals Correction to: Bioactivities of Penicillium citrinum isolated from a medicinal plant Swertia chirayita

2021 ◽  
Vol 203 (9) ◽  
pp. 5843-5843
Author(s):  
Hemant Sharma ◽  
Arun Kumar Rai ◽  
Rajen Chettri ◽  
Poonam Singh Nigam
Keyword(s):  
Medicinal Plant ◽  
Penicillium Citrinum ◽  
Swertia Chirayita

scholarly journals QUANTIFICATION OF MAJOR PHYTOCHEMICALS OF SWERTIA CHIRAYITA, A MEDICINAL PLANT FROM NEPAL

1970 ◽  
Vol 17 ◽  
pp. 59-68 ◽  
Author(s):  
Susanna Phoboo ◽  
Marcia Da Silva Pinto ◽  
Prasanta C. Bhowmik ◽  
Pramod Kumar Jha ◽  
Kalidas Shetty
Keyword(s):  
Medicinal Plant ◽  
High Performance ◽  
Cultivated Plants ◽  
Swertia Chirayita ◽  
Plant Parts ◽  
Central Nepal ◽  
Significant Difference ◽  
Cultivated Plant ◽  
Array Detection ◽  
Important Medicinal Plant

Swertia chirayita is an important medicinal plant from Nepal with anti-diabetic, anti-pyretic, anti-malarial and anti-inflammatory potential and used in therapeutic herbal preparations in parts of South Asia. The main phytochemicals in crude aqueous and ethanolic extracts of different plant parts of Swertia chirayita collected from nine different districts of Nepal representing West, East and Central Nepal were quantified using HPLC/DAD (High Performance Liquid Chromatography-Diode Array Detection). The quantities of these phytochemicals were also compared between wild and cultivated plant parts of Swertia chirayita. Amarogentin, mangiferin, swertiamarin were the main phytochemicals in all extracts. The highest quantity of all the three phytochemicals was found in IL (inflorescence and leaf mixture) of all the collected plants samples. There was no significant difference in the amounts of these three phytochemicals between extracts from wild and cultivated plants. The result from this study substantiates the validity of cultivated Swertia chirayita for medicinal purposes and trade.Key words:  Swertia chirayita; HPLC/DAD; Wild; Cultivated; Phytochemicals; Quantification; Mangiferin; Swertiamarin; AmarogentinDOI: 10.3126/eco.v17i0.4118EcoprintAn International Journal of EcologyVol. 17, 2010Page: 59-68Uploaded date: 3 January, 2011

scholarly journals Trade and Sustainable Conservation of Swertia chirayita (Roxb. ex Fleming) h. Karst in Nepal

1970 ◽  
Vol 11 ◽  
pp. 125-132 ◽  
Author(s):  
Susanna Phoboo ◽  
Pramod Kumar Jha
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Sustainable Use ◽  
Current Status ◽  
Fiscal Year ◽  
Sustainable Harvesting ◽  
Swertia Chirayita ◽  
Extensive Collection ◽  
Sustainable Conservation ◽  
Important Medicinal Plant

Swertia chirayita (Roxb. ex Fleming) H. Karst is one of the most important high-value medicinal plants of Nepal prized for its anti-diabetic, antimalarial, hepato-protective and anti-inflammatory properties. It is widely used as an important ingredient in medicinal preparations in Ayurveda, Unnani, Siddha, Tibetan and Chinese traditional medicine. Nepal is one of the main exporters of this important plant and the most significant importers are India and China. The large scale export of this plant has made it vulnerable in Nepal and there is an immediate need for its conservation. Detailed information on the current status of trade of this plant in Nepal is necessary to understand and make assumptions on its current market standing or future predictions of demand. S. chirayita is traded in 61 of the 75 districts of Nepal and constituted about 3% in the total medicinal plant traded in the fiscal year 2008-2009. Due to extensive collection of S. chirayita from the wild, there is a need for exploring alternative conservation options in order to preserve the remaining wild population. Sustainable harvesting and cultivation can help in its conservation. This paper briefly reviews the state of trade and sustainable conservation of this important medicinal plant in Nepal. Key words: Swertia chirayita; trade; cultivation; sustainable use; sustainable harvesting DOI: 10.3126/njst.v11i0.4134Nepal Journal of Science and Technology 11 (2010) 125-132

scholarly journals Two Polyketides Produced by Endophytic Penicillium citrinum DBR-9 From Medicinal Plant Stephania kwangsiensis and Their Antifungal Activity Against Plant Pathogenic Fungi

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984679 ◽  
Author(s):  
Haiyu Luo ◽  
Zhen Qing ◽  
Yecheng Deng ◽  
Zhiyong Deng ◽  
Xia’an Tang ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Membrane Integrity ◽  
Pathogenic Fungi ◽  
Significant Inhibition ◽  
Penicillium Citrinum ◽  
Plant Pathogenic Fungi ◽  
Fungal Cell ◽  
Cell Membrane Integrity ◽  
Chinese Medicinal Plant

Endophytic fungi, especially those found in medicinal plants, are widely studied as producers of secondary metabolites of biotechnological interest. In this study, on the basis of an activity-directed isolation method and spectroscopic analysis, two active polyketides, citrinin (1) and emodin (2), were isolated and identified from the fermentation of the endophytic fungus Penicillium citrinum DBR-9. This fungus was isolated from the root tubers of the traditional Chinese medicinal plant Stephania kwangsiensis. In vitro antifungal assay showed that the two polyketides displayed significant inhibition on hypha growth of tested plant pathogenic fungi with IC50 values ranging from 3.1 to 123.1 μg/mL and 3.0 to 141.0 μg/mL, respectively. In addition, the mechanism of the effects of emodin (2) on the pathogen revealed it could affect the colony morphology, destroy cell membrane integrity, and influence the protein synthesis of the tested fungal cell. This work is the first report of two polyketides-producing endophytic P. citrinum DBR-9 from the medicinal plant S. kwangsiensis. Our results present new opportunities to deeply understand the potential of these two polyketides as natural antifungal agents to control phytopathogens in agriculture.

scholarly journals PHÂN LẬP VÀ ĐÁNH GIÁ KHẢ NĂNG ĐỐI KHÁNG CỦA CÁC CHỦNG NẤM TỪ ĐẤT NÔNG NGHIỆP

2021 ◽  
Vol 130 (1A) ◽  
Author(s):  
Lê Thanh Toàn ◽  
Phạm Văn Hướng
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Solani ◽  
Penicillium Citrinum

Tác giả đã phân lập được 39 chủng nấm từ đất vườn trồng cây có múi, trong đó 7 chủng nấm rễ và vùng rễ có khả năng đối kháng tốt với Fusarium solani và 8 chủng nấm rễ và vùng rễ đối kháng tốt với Rhizoctonia solani và đều đạt hiệu quả đối kháng trên 60% ở 7 ngày sau đặt khoanh nấm. Trong đó, chủng nấm Penicillium citrinum – một loại nấm rễ nội sinh trong rễ cây trồng – cho hiệu quả đối kháng 60,63% với F. solani và 73,13% với R. solani.

scholarly journals Intracellular Siderophore Detection in an Egyptian, Cobalt-Treated F. solani Isolate Using SEM-EDX with Reference to its Tolerance

2017 ◽  
Vol 66 (2) ◽  
pp. 235-243 ◽  
Author(s):  
Farrag M. Rasha
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Fusarium Solani ◽  
X Ray ◽  
Cobalt Uptake ◽  
Molecular Studies ◽  
Control Regime ◽  
Co Concentrations ◽  
Scanning Electron

An Egyptian, plant pathogenic Fusarium solani isolate was grown on cobalt concentrations of 0, 50, 200, 500, 800, and 1000 ppm. The isolate survived concentrations up to 800 ppm, however failed to grow at 1000 ppm. Morphology and elemental analysis of the isolate under the investigated Co concentrations were studied using Scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). The isolate reserved its morphology up to a concentration of 200 ppm. Morphological distortions were dramatic at 500 and 800 ppm. EDX detected Co uptake through the hyphae, microconidia, macroconidia, and chlamydospores. Iron, calcium, and potassium were also detected. EDX results showed a linear relationship between Co% and Fe% up to a concentration of 500 ppm reflecting the possible ability of the isolate to synthesize intracellular siderophores storing iron and their release out of the vacuoles. The participation of such siderophores in conferring tolerance against cobalt is discussed. At 800 ppm, the % of Fe was greatly reduced with an accompanying increase in morphological distortions and absence of microconidia. Increasing the implicated cobalt concentrations resulted in increasing the percentages of the chelated cobalt reflecting the possible implication of the cell wall as well as extracellular siderophores in the uptake of cobalt. The current results recommend the absence of cobalt in any control regime taken to combat the investigated F. solani isolate and highlights the accomplishment of biochemical, ultrastructural, and molecular studies on such isolate to approve the production of siderophores and the role of cell wall in cobalt uptake.

scholarly journals ISOLASI DAN IDENTIFIKASI KAPANG PADA PINDANG BANDENG (Chanos chanos) PRESTO

2019 ◽  
Vol 2 (1) ◽  
pp. 15
Author(s):  
Resmi Rumenta Siregar
Keyword(s):  
Yeast Extract ◽  
Rose Bengal ◽  
Fusarium Solani ◽  
Penicillium Citrinum ◽  
Malt Extract Agar ◽  
Malt Extract ◽  
Chanos Chanos ◽  
Extract Agar ◽  
Fusarium Sp ◽  
Yeast Extract Agar

Ikan pindang adalah salah satu olahan yang sangat disukai oleh masyarakat Indonesia. Hal ini dapat dilihat dari produksi ikan pindang yang setiap tahunnya mengalami peningkatan. Sebagai contoh di Kabupaten Bogor, produksi ikan pindang pada tahun 2013 sebesar 3.643,56 ton, meningkat menjadi 10.334,44ton pada tahun 2015. Ikan pindang disisi lain, sangat mudah mengalami kemunduran mutu disebabkan masih tingginya kadar air, pengemasan yang tidak memenuhi standar serta proses pengolahan yang pada umumnya kurang menerapkan prinsip sanitasi yang baik. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi kapang yang tumbuh pada bandeng presto. Sampel Bandeng presto diambil dari CV. Cindy Group. Kapang diisolasi dengan metode pengenceran bertingkat menggunakan media DRBC (Dichloran Rose Bengal Chloramphenicol Agar), kemudian diidentifikasi secara morfologi menggunakan media Malt Extract Agar dan Czapek’s Yeast Extract Agar. Nilai Aktivitas air (aw) bandeng presto memiliki kisaran rata-rata 0,96 – 0,97. Secara makroskopis terlihat adanya pertubuhan kapang pada permukaan ikan bandeng presto setelah penyimpanan selama 3 hari pada suhu ruang (20-250C). Sebanyak 5 isolat kapang diisolasi dari ikan pindang sampel bandeng presto. Hasil identifikasi secara mikroskopis diketahui bahwa kapang yang tumbuh ada ikan pindang tersebut adalah spesies Penicillium citrinum, Eurotium chevalieri, Fusarium solani, Fusarium sp, dan Cladosporium sp. Kadar aw ikan pindang resto yang masih tinggi (0,96-0,97) menyebabkan ikan pindang mengalami pembusukan yang diakibatkan oleh bakteri.  

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