Development of a double-monitoring method for the determination of total antioxidant capacity as ascorbic acid equivalent using CUPRAC assay with RP-HPLC and digital image-based colorimetric detection

2021 ◽  
Author(s):  
Tülay Borahan ◽  
Ayça Girgin ◽  
Nihan Atsever ◽  
Buse Tuğba Zaman ◽  
Dotse Selali Chormey ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Digital Image ◽  
Total Antioxidant Capacity ◽  
Colorimetric Detection ◽  
Monitoring Method ◽  
Rp Hplc ◽  
Total Antioxidant

scholarly journals Total Antioxidant Capacity and Lipid Peroxidation Status in Cervical Cancer Patients Compared with Women Without Cervical Cancer in Bangladesh

2021 ◽  
Vol 19 (4) ◽  
Author(s):  
Taslima Nigar ◽  
Annekathryn Goodman ◽  
Shahana Pervin
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Cancer Patients ◽  
Total Antioxidant Capacity ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Total Antioxidant

Abstract Purpose Over the past several decades, research has suggested reactive oxygen species act as cofactors for cervical cancer development. The aim of this study is to evaluate the antioxidant and lipid peroxidation status in cervical cancer patients in Bangladesh. Methods From December 2017 to 2018, a cross-sectional observational study was conducted on 50 cervical cancer patients and 50 controls. Plasma levels of lipid peroxidation and total antioxidant capacity were measured. The Student’s t test was used for statistical analysis. P values less than 0.05 were taken as a level of significance. Results There was a significant reduction in total antioxidant levels in patients with cervical cancer, 972.77 ± 244.22 SD µmol equivalent to ascorbic acid/L, compared to normal controls, 1720.13 ± 150.81 SD µmol equivalent to ascorbic acid/L (P < 0.001). Levels of lipid peroxidation were found to be significantly higher in cervical cancer, 7.49 ± 2.13 SD µmol/L, than in women without cervical cancer, 3.28 ± 0.58 SD µmol/L (P < 0.001). The cervical cancer patients had significantly higher levels of oxidative stress index (0.83 ± 0.31) in comparison to controls (0.19 ± 0.04) (P < 0.001). Conclusion There was an increased oxidative stress index due to imbalance between lipid peroxidation generation and total antioxidant capacity in cervical cancer patients. Further studies are needed to explore the role of oxidative stress as a cofactor for cervical carcinogenesis.

Determination of Satiety, Glycaemic Profiles, Total Antioxidant Capacity and Postprandial Glycemic Responses of Minimally Refined Sugar and Others in Malaysian Healthy Adults

Nutrition ◽  
2021 ◽  
pp. 111551
Author(s):  
Azrina Azlan ◽  
Samarghand Ebadi ◽  
Barakatun Nisak Mohd Yusof ◽  
Nur Maziah Hanum Othman ◽  
David Kannar ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Healthy Adults ◽  
Total Antioxidant

scholarly journals Antioxidant and cytotoxic activities of Ageratum conyzoides stems

2013 ◽  
Vol 2 (2) ◽  
pp. 33-37 ◽  
Author(s):  
Fatema Nasrin
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reducing Power ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ageratum Conyzoides ◽  
Reducing Ability ◽  
Ic50 Value ◽  
Total Antioxidant

Modern civilization is facing more than hundreds of disorders associated with free radicals and natural antioxidants from non-edible plants are gaining importance to fight these disorders. The intention of this report is to evaluate a well known medicinal weed Ageratum conyzoides stems for its antioxidant and cytotoxic Effects. Antioxidant potentiality of the crude methanolic extract of the Ageratum conyzoides (AC) stems was investigated on DPPH scavenging activity, reducing ability, total antioxidant capacity as well as total phenolic contents. Cytotoxic study was done by brine shrimp lethality bioassay and vincristin sulphate was used as standard. The total phenols and total antioxidant capacity of AC was found to be 38.125 ± 2.01mg/g equivalent of gallic acid and 333.37 ± 4.22mg/gm equivalent of ascorbic acid, respectively. The percentage (%) scavenging of DPPH free radical of the extract was found to be concentration dependent with IC50 value 46.01 ± 2.23µg/ml while IC50 value of standard ascorbic acid was found to be 29.56 ± 0.11?g/ml. The reducing power of AC was found to be concentration dependent. The cytotoxicity exhibited by AC was found promising with LC50 value 1.32?g/ml, comparing with the LC50 (0.689?g/ml) values of vincristin sulphate. The present investigation suggests that Ageratum conyzoides possesses remarkable antioxidant and cytotoxic property.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13195 International Current Pharmaceutical Journal 2013, 2(2): 33-37

scholarly journals A novel colorimetric paper sensor based on the layer-by-layer assembled multilayers of surfactants for the sensitive and selective determination of total antioxidant capacity

RSC Advances ◽  
2019 ◽  
Vol 9 (49) ◽  
pp. 28598-28608 ◽  
Author(s):  
Siriboon Mukdasai ◽  
Pikaned Uppachai ◽  
Supalax Srijaranai
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Food Samples ◽  
Layer By Layer ◽  
Selective Determination ◽  
Total Antioxidant ◽  
Paper Sensor

A new colorimetric paper sensor, (TBABr/SDS)3/PAD, was successfully fabricated to detect TAC. Good selectivity and sensitivity toward TAC were achieved. The (TBABr/SDS)3/PAD sensor has been applied for TAC detection in food samples.

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