Aeration strategy: a need for very high ethanol performance in Saccharomyces cerevisiae fed-batch process

2004 ◽  
Vol 63 (5) ◽  
pp. 537-542 ◽  
Author(s):  
S. Alfenore ◽  
X. Cameleyre ◽  
L. Benbadis ◽  
C. Bideaux ◽  
J.-L. Uribelarrea ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Process ◽  
Fed Batch ◽  
High Ethanol ◽  
Very High

scholarly journals Quantitative evaluation of yeast's requirement for glycerol formation in very high ethanol performance fed-batch process

2010 ◽  
Vol 9 (1) ◽  
pp. 36 ◽  
Author(s):  
Julien Pagliardini ◽  
Georg Hubmann ◽  
Carine Bideaux ◽  
Sandrine Alfenore ◽  
Elke Nevoigt ◽  
...  
Keyword(s):  
Quantitative Evaluation ◽  
Batch Process ◽  
Fed Batch ◽  
Glycerol Formation ◽  
High Ethanol ◽  
Very High

Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process

2002 ◽  
Vol 60 (1-2) ◽  
pp. 67-72 ◽  
Author(s):  
Alfenore S. ◽  
Molina-Jouve C. ◽  
Guillouet S. ◽  
Uribelarrea J.-L. ◽  
Goma G. ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Feeding Strategy ◽  
Batch Process ◽  
Fed Batch

scholarly journals Auxotrophic Mutations Reduce Tolerance of Saccharomyces cerevisiae to Very High Levels of Ethanol Stress

2015 ◽  
Vol 14 (9) ◽  
pp. 884-897 ◽  
Author(s):  
Steve Swinnen ◽  
Annelies Goovaerts ◽  
Kristien Schaerlaekens ◽  
Françoise Dumortier ◽  
Pieter Verdyck ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Tolerance ◽  
Stress Factors ◽  
Causative Mutation ◽  
Ethanol Stress ◽  
Limiting Factor ◽  
Ethanol Sensitivity ◽  
Content Type ◽  
High Ethanol ◽  
Very High

ABSTRACTVery high ethanol tolerance is a distinctive trait of the yeastSaccharomyces cerevisiaewith notable ecological and industrial importance. Although many genes have been shown to be required for moderate ethanol tolerance (i.e., 6 to 12%) in laboratory strains, little is known of the much higher ethanol tolerance (i.e., 16 to 20%) in natural and industrial strains. We have analyzed the genetic basis of very high ethanol tolerance in a Brazilian bioethanol production strain by genetic mapping with laboratory strains containing artificially inserted oligonucleotide markers. The first locus contained theura3Δ0mutation of the laboratory strain as the causative mutation. Analysis of other auxotrophies also revealed significant linkage forLYS2,LEU2,HIS3, andMET15. Tolerance to only very high ethanol concentrations was reduced by auxotrophies, while the effect was reversed at lower concentrations. Evaluation of other stress conditions showed that the link with auxotrophy is dependent on the type of stress and the type of auxotrophy. When the concentration of the auxotrophic nutrient is close to that limiting growth, more stress factors can inhibit growth of an auxotrophic strain. We show that very high ethanol concentrations inhibit the uptake of leucine more than that of uracil, but the 500-fold-lower uracil uptake activity may explain the strong linkage between uracil auxotrophy and ethanol sensitivity compared to leucine auxotrophy. Since very high concentrations of ethanol inhibit the uptake of auxotrophic nutrients, the active uptake of scarce nutrients may be a major limiting factor for growth under conditions of ethanol stress.

scholarly journals Co-Production of Isobutanol and Ethanol from Prairie Grain Starch Using Engineered Saccharomyces cerevisiae

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 150
Author(s):  
Xiaodong Liu ◽  
Ebele Unaegbunam ◽  
David T. Stuart
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Simultaneous Saccharification And Fermentation ◽  
Batch Process ◽  
Energy Crop ◽  
Combustion Engines ◽  
Fed Batch ◽  
Fuel Distribution ◽  
Grain Starch ◽  
Engineered Saccharomyces Cerevisiae ◽  
Simultaneous Saccharification

Isobutanol is an important and valuable platform chemical and an appealing biofuel that is compatible with contemporary combustion engines and existing fuel distribution infrastructure. The present study aimed to compare the potential of triticale, wheat and barley starch as feedstock for isobutanol production using an engineered strain of Saccharomyces cerevisiae. A simultaneous saccharification and fermentation (SSF) approach showed that all three starches were viable feedstock for co-production of isobutanol and ethanol and could produce titres similar to that produced using purified sugar as feedstock. A fed-batch process using triticale starch yielded 0.006 g isobutanol and 0.28 g ethanol/g starch. Additionally, it is demonstrated that Fusarium graminearum infected grain starch contaminated with mycotoxin can be used as an effective feedstock for isobutanol and ethanol co-production. These findings demonstrate the potential for triticale as a purpose grown energy crop and show that mycotoxin-contaminated grain starch can be used as feedstock for isobutanol biosynthesis, thus adding value to a grain that would otherwise be of limited use.

scholarly journals Effect of Myclobutanil Pesticide on the Physiological Behavior of Two Newly Isolated Saccharomyces cerevisiae Strains during Very-High-Gravity Alcoholic Fermentation

2019 ◽  
Vol 7 (12) ◽  
pp. 666 ◽  
Author(s):  
Antonia Terpou ◽  
Maria Dimopoulou ◽  
Aikaterini Belka ◽  
Stamatina Kallithraka ◽  
George-John E. Nychas ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Substrate Uptake ◽  
High Gravity ◽  
Protective Agent ◽  
Grape Must ◽  
Very High Gravity ◽  
High Ethanol ◽  
Very High ◽  
Grape Musts

Yeasts are able to act as biosorbents, as their cell wall includes several components capable of binding organic xenobiotic compounds that can potentially be removed during various fermentation processes. In the present investigation, two novel Saccharomyces cerevisiae strains (LMBF-Y 16 and LMBF-Y-18), previously isolated from grapes, were studied regarding their physiological behavior (dry cell weight—DCW production, substrate uptake, and ethanol and glycerol biosynthesis) during fermentations of grape must, in some cases enriched with commercial glucose and fructose (initial total sugar concentration approximately 150 and 250 g/L, respectively). Myclobutanil (a chiral triazole fungicide broadly used as a protective agent of vine) was also added to the culture media at various concentrations in order to assess the ability of the yeasts to simultaneously perform alcoholic fermentations and detoxify the medium (i.e., to remove the fungicide). In the first set of experiments and for both tested strains, trials were carried out in either 250 mL or 2.0 L agitated shake flasks in either synthetic glucose-based experiments or grape musts. Since the results obtained in the trials where the cultures were placed in 2.0 L flasks with grape musts as substrates were superior in terms of both DCW and ethanol production, these experimental conditions were selected for the subsequent studies. Both strains showed high fermentative efficiency, producing high amounts of DCW (9.5–10.5 g/L) in parallel with high ethanol production, which in some cases achieved values very close to the maximum theoretical ethanol production yield (≈0.49 g of ethanol per g of sugar). When using grape must with initial total sugars at approximately 250 g/L (very high gravity fermentation media, close to winemaking conditions), significantly high ethanol quantities (i.e., ranging between 105 and 123 g/L) were produced. Myclobutanil addition slightly negatively affected sugar conversion into ethanol; however, in all cases, ethanol production was very satisfactory. A non-negligible myclobutanil removal during fermentation, which ranged between 5%–27%, as a result of the adsorptive or degradative capacity of the yeast was also reported. The presence of myclobutanil had no effect on DCW production and resulted in no significant differences in the biosynthesis of glycerol. Therefore, these newly isolated yeast strains could be excellent candidates for simultaneous high ethanol production and parallel pesticide removal in a general biorefinery concept demonstrating many environmental benefits.

Alcoholic Fermentation with Flocculant Saccharomyces cerevisiae in Fed-Batch Process

2013 ◽  
Vol 172 (3) ◽  
pp. 1623-1638 ◽  
Author(s):  
Carla Zanella Guidini ◽  
Líbia Diniz Santos Marquez ◽  
Helisângela de Almeida Silva ◽  
Miriam Maria de Resende ◽  
Vicelma Luiz Cardoso ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alcoholic Fermentation ◽  
Batch Process ◽  
Fed Batch
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